Expression Profiling of Cassava Storage Roots Reveals an Active Process of Glycolysis/Gluconeogenesis

作     者：Jun Yang Dong An Peng Zhang 

作者机构：Shanghai Center for Cassava Biotechnology National Laboratory of Plant Molecular Genetics Institute of Plant Physiology & Ecology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200032 China Key Laboratory of Synthetic Biology Institute of Plant Physiology & Ecology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200032 China Shanghai Chenshan Plant Science Research Center the Chinese Academy of Sciences Chenshan Botanical Garden Shanghai 201602 China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2011年第53卷第3期

页      面：193-211页

核心收录：

学科分类：081703[工学-生物化工] 08[工学] 0817[工学-化学工程与技术] 09[农学] 0901[农学-作物学] 0836[工学-生物工程] 082203[工学-发酵工程] 0822[工学-轻工技术与工程] 

基　　金：supported by grants from the National Basic Research Program (2010CB126605) the National High Technology Research and Development Program of China(2009AA10Z102) the Earmarked Fund for Modern Agroindustry Technology Research System (nycytx-17) the Chinese Academy of Sciences (KSCX2-EW-J-12) Shanghai Municipal Afforestation & City Appearance and Environmental Sanitation Administration (G102410) 

主　　题：CASSAVA ROOTS (Botany) -- Formation GLYCOLYSIS GLUCONEOGENESIS LACTATE dehydrogenase REVERSE transcriptase polymerase chain reaction PLANT gene expression OLIGONUCLEOTIDE arrays 

摘      要：Mechanisms related to the development of cassava storage roots and starch accumulation remain largely unknown. To evaluate genome-wide expression patterns during tuberization, a 60 mer oligonucleotide microarray representing 20 840 cassawl genes was designed to identify differentially expressed transcripts in fibrous roots, developing storage roots and mature storage roots. Using a random variance model and the traditional twofold change method for statistical analysis, 912 and 3 386 upregulated and downregulated genes related to the three developmental phases were identified. Among 25 significantly changed pathways identified, glycolysis/gluconeogenesis was the most evident one. Rate-limiting enzymes were identified from each individual pathway, for example, enolase, L-lactate dehydrogenase and aldehyde dehydrogenase for glycolysis/gluconeogenesis, and ADP-glucose pyrophosphorylase, starch branching enzyme and glucan phosphorylase for sucrose and starch metabolism. This study revealed that dynamic changes in at least 16% of the total transcripts, including transcription fac- tors, oxidoreductases/transferases/hydrolases, hormone-related genes, and effectors of homeostasis. The reliability of these differentially expressed genes was verified by quantitative real-time reverse transcription-polymerase chain reaction. Tlhese studies should facilitate our understanding of the storage root formation and cassava improvement.