Optimization of enzymatic hydrolysis of cassava to obtain fermentable sugars

作     者：Renata M.COLLARES Luiza V.S.MIKLASEVICIUS Mariana M.BASSACO Nina P.G.SALAU Marcio A.MAZUTTI Dilson A.BISOGNIN Lisiane M.TERRA 

作者机构：Department of Chemical EngineeringFederal University of Santa MariaAv.Roraima 1000Santa Maria 97105-900Brazil Department of HorticultureFederal University of Santa MariaAv.Roraima 1000Santa Maria 97105-900Brazil 

出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)

年 卷 期：2012年第13卷第7期

页      面：579-586页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 081703[工学-生物化工] 1001[医学-基础医学(可授医学、理学学位)] 0905[农学-畜牧学] 08[工学] 0817[工学-化学工程与技术] 0906[农学-兽医学] 0836[工学-生物工程] 082203[工学-发酵工程] 0822[工学-轻工技术与工程] 

基　　金：Project supported by the National Council for Scientific and Technological Development(CNPq) Coordination for the Improvement of Higher Level Personnel(CAPES),Brazil 

主　　题：Cassava Enzymatic hydrolysis Enzyme reactor Starch 

摘      要：This work evaluates the enzymatic hydrolysis of starch from cassava using pectinase,α-amylase,and amyloglucosidase.A central composite rotational design(CCRD)was carried out to evaluate the effects of amyloglucosidase,pectinase,reaction time,and solid to liquid *** the experiments were carried out in a bioreactor with working volume of 2 *** 98%efficiency hydrolysis was obtained,resulting in a concentration of total reducing sugar released of 160 g/*** was concluded that pectinase improved the hydrolysis of starch from *** time was found to be significant until 7 h of reaction.A solid to liquid ratio of 1.0 was considered suitable for hydrolysis of starch from *** was a significant variable in the process:after its addition to the reaction media,a 30%-50%increase in the amount of total reducing sugar released was *** optimal conditions the maximum productivity obtained was 22.9 g/(L.h).