检索结果-南通市图书馆

Journal of Integrative Plant Biology 2008年第5期50卷 581-588页

作者： Jie Zhang Liang-Sheng Wang Jin-Ming Gao Qing-Yan Shu Chong-Hui Li Juan Yao Qing Hao Jing-Jing Zhang College of Science Northwest A&F University Shaanxi 712100 China Beijing Botanical Garden Institute of Botany the Chinese Academy of Sciences Beijing 100093 China

Petal coloration and pigment components in 12 American crape myrtle cultivars （Lagerstroemia indica x Lagerstroemia fauriei） and five Chinese crape myrtle cuItivars （L. indica hybrids） were studied. Color was measured by CIEL^＊a^＊b^＊ scale and anthocyanin composition of crape myrtle was determined using high-performance liquid chromatography coupled to photodiode array detection and electrospray ionization mass spectrometry. The presence of the previously reported delphinidin 3-O-glucoside, petunidin 3-O-glucoside and malvidin 3-O-glucoside were confirmed. Cyanidin 3-O-glucoside was identified in crape myrtle for the first time. We explored the relationship between petal color and anthocyanin contents by multiple linear regression analyses. The results indicated that total fiavones and fiavonols were important variables and contributed to blue-enhancing in crape myrtle. Based on anthocyanins and co-pigments analysis, flower color breeding in crape myrtle towards true-red and blue were discussed.

关键词： anthocyanin breeding co-pigmentation crape myrtle flower color high-performance liquid chromatography and electrospray ionization mass spectrometry.

Transcriptome and Metabolic Profiling Provides Insigh into Betalain Biosynthesis and Evolution in Mirabilis jalapa

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2018年第1期11卷 189-204页

作者： Guy Polturak Uwe Heinig Noam Grossman Maor Battat Dena Leshkowitz Sergey Malitsky liana Rogachev~ and Asaph Aharoni Department of Plant and Environmental Sciences Weizmann Institute of Science 234 Herzl Street Rehovot 7610001 Israel Bioinformatics Unit Life Sciences Core Facilities Weizmann Institute of Science 234 Herzl Street Rehovot 7610001 Israel

Betalains are tyrosine-derived pigments that occur solely in one plant order, the Caryophyllales, where they largely replace the anthocyanins in a mutually exclusive manner. In this study, we conducted multi-species transcriptome and metabolic profiling in Mirabilisjalapa and additional betalain-producing species to identify candidate genes possibly involved in betalain biosynthesis. Among the candidates identified, betalainrelated ytochrome P450 and glucosyltransferase-type genes, which catalyze tyrosine hydroxylation or （hydroxy）cinnamoyl-glucose formation, respectively, were further functionally characterized. We detected the expression of genes in the flavonoid/anthocyanin biosynthetic pathways as well as their metabolite intermediates in betalain-accumulating M. jalapa flowers, and found that the anthocyanin-related gene ANTHOCYANIDIN SYNTHASE （MjANS） is highly expressed in the betalain-accumulating petals. However, it appears that MjANS contains a significant deletion in a region spanning the corresponding enzyme active site. These findings provide novel insights into betalain biosynthesis and a possible explanation for how anthocyanins have been lost in this plant species. Our study also implies a complex, non-uniform history for the loss of anthocyanin production across betalain producers, previously assumed to be strictly due to diminished expression of anthocyanin-related genes.

关键词： betalain anthocyanin caryophyllales plant pigment biosynthesis secondary metabolism

Phenylpropanoid Biosynthesis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2010年第1期3卷 2-20页

作者： Thomas Vogt Leibniz-lnstitute of Plant Biochemistry Department of Secondary Metabolism Weinberg 3 D-06120 Halle (Saale) Germany

The general phenylpropanoid metabolism generates an enormous array of secondary metabolites based on the few intermediates of the shikimate pathway as the core unit. The resulting hydroxycinnamic acids and esters are am- plified in several cascades by a combination of reductases, oxygenases, and transferases to result in an organ and devel- opmentally specific pattern of metabolites, characteristic for each plant species. During the last decade, methodology driven targeted and non-targeted approaches in several plant species have enabled the identification of the participating enzymes of this complex biosynthetic machinery, and revealed numerous genes, enzymes, and metabolites essential for regulation and compartmentation. Considerable success in structural and computational biology, combined with the an- alytical sensitivity to detect even trace compounds and smallest changes in the metabolite, transcript, or enzyme pattern, has facilitated progress towards a comprehensive view of the plant response to its biotic and abiotic environment. Trans- genic approaches have been used to reveal insights into an apparently redundant gene and enzyme pattern required for functional integrity and plasticity of the various phenylpropanoid biosynthetic pathways. Nevertheless, the function and impact of all members of a gene family remain to be completely established. This review aims to give an update on the various facets of the general phenylpropanoid pathway, which is not only restricted to common lignin or flavonoid biosynthesis, but feeds into a variety of other aromatic metabolites like coumarins, phenolic volatiles, or hydrolyzable tannins.

关键词： Phenylpropanoid biosynthetic pathway flavonoid anthocyanin tannin coumarin volatiles lignin.

Production of anthocyanins in metabolically engineered microorganisms:Current status and perspectives

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Synthetic and Systems Biotechnology 2017年第4期2卷 259-266页

作者： Jian Zha Mattheos A.G.Koffas Department of Chemical and Biological Engineering Center for Biotechnology and Interdisciplinary StudiesRensselaer Polytechnic InstituteTroyNYUSA Department of Biological Sciences Center for Biotechnology and Interdisciplinary StudiesRensselaer Polytechnic InstituteTroyNYUSA

Microbial production of plant-derived natural products by engineered microorganisms has achieved great success thanks to large extend to metabolic engineering and synthetic biology.anthocyanins,the water-soluble colored pigments found in terrestrial plants that are responsible for the red,blue and purple coloration of many flowers and fruits,are extensively used in food and cosmetics industry;however,their current supply heavily relies on complex extraction from plant-based materials.A promising alternative is their sustainable production in metabolically engineered microbes.Here,we review the recent progress on anthocyanin biosynthesis in engineered bacteria,with a special focus on the systematic engineering modifications such as selection and engineering of biosynthetic enzymes,engineering of transportation,regulation of UDP-glucose supply,as well as process optimization.These promising engineering strategies will facilitate successful microbial production of anthocyanins in industry in the near future.

关键词： anthocyanin Enzyme engineering Metabolic engineering Microbial production

Prefoldins Negatively Regulate Cold Acclimation in Arabidopsis thaliana by Promoting Nuclear Proteasome-Mediated HY5 Degradation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2017年第6期10卷 791-804页

作者： Carlos Perea-Resa Miguel A. Rodriguez-Milla Elisa Iniesto Vicente Rubio Julio Salinas Departamento de Biologia Medioambiental Centro de Investigaciones Biologicas CSIC 28040 Madrid Spain Departamento de Genetica Molecular de Plantas Centro Nacional de Biotecnologia CSIC 28049 Madrid Spain present address: Department of Molecular Biology Massachusetts General Hospital Boston MA 02214 USA Present address: Unidad de Biotecnologia Celular Instituto de Salud Garlos Ⅲ 28222 Madrid Spain

The process of cold acclimation is an important adaptive response whereby many plants from temperate regions increase their freezing tolerance after being exposed to low non-freezing temperatures. The correct development of this response relies on proper accumulation of a number of transcription factors that regulate expression patterns of cold-responsive genes. Multiple studies have revealed a variety of molecular mechanisms involved in promoting the accumulation of these transcription factors. Interestingly, however, the mechanisms implicated in controlling such accumulation to ensure their adequate levels remain largely unknown. In this work, we demonstrate that prefoldins （PFDs） control the levels of HY5, an Arabidopsis transcription factor with a key role in cold acclimation by activating anthocyanin biosynthesis, in response to low temperature. Our results show that, under cold conditions, PFDs accumulate into the nucleus through a DELLA-dependent mechanism, where they interact with HY5, triggering its ubiquitination and subsequent degradation. The degradation of HY5 would result, in turn, in anthocyanin biosynthesis attenuation, ensuring the accurate development of cold acclimation. These findings uncover an unanticipated nuclear function for PFDs in plant responses to abiotic stresses.

关键词： prefoldins cold acclimation Arabidopsis HY5 anthocyanin DELLAs

The Strawberry Fruit Fra a Allergen Functions in Flavonoid Biosynthesis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2010年第1期3卷 113-124页

作者： Cristina Munoz Thomas Hoffmann Nieves Medina Escobar Felix Ludemann Miguel A. Botella Victoriano Valpuesta Wilfried Schwab Departamento de Biologia Molecular y Bioquimica Universidad de Malaga 29071 Malaga Spain Biomolecular Food Technology Technische Universit~t M0nchen Hochfeldweg 1 85354 Freising Germany

The strawberry Fra a I allergen is a homolog of the major birch pollen allergen Bet v 1. It is synthesized by red ripe fruits of Fragaria x ananassa while white fruits of a mutant genotype, which is known to be tolerated by individuals affected by allergy, are devoid of it. Proteomic analyses have shown that Fra a 1 is down-regulated in the tolerated white- fruited genotype along with enzymes of the anthocyanin pigment pathway. In this study, we report the spatial and tem- poral expression of three Fra a genes that encode different isoforms, and the transient RNAi-mediated silencing of the Fra a genes in strawberry fruits of the red-fruited cultivar Elsanta with an ihpRNA construct. As a consequence of reduced levels of Fra a mRNAs, fruits were obtained that produced significantly decreased levels of anthocyanins and upstream metabolites. This effect is consistent with the parallel down-regulation of the phenylalanine ammonia lyase （FaPAL） and to a lesser extent of the chalcone synthase （FaCHS） transcript levels also found in these fruits. In naturally occurring white- fruited genotypes of F. chiloensis and F. vesca, Fra a transcript levels are higher than those of the red-fruited varieties, likely to compensate for the low expression levels of FaPAL and FaCHS in these mutant genotypes. The results demonstrate that Fra a expression is directly linked to flavonoid biosynthesis and show that the Fra a allergen has an essential biological function in pigment formation in strawberry fruit.

关键词： Fragaria flavonoid anthocyanin phenylpropanoid allergen pathogenesis-related proteins.

富含anthocyanin的水果提取物对血管内皮细胞产生PGE_2的影响研究

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

卫生研究 2005年第5期34卷 581-584页

作者：韩光亮李翠梅 Mazza G 杨晓光中国疾病预防控制中心营养与食品安全所北京100050 华北煤炭医学院药物研究所加拿大太平洋农业与食品研究中心

目的研究富含anthocyanin的水果提取物对血管内皮细胞产生PGE2的影响.方法将人类正常的血管内皮细胞CRL-2606在Kaighn's F12K培养基中(补加有10%FBS,0.1mg/ml肝素,0.03mg/ml的ECGS,50μg/ml链霉素和500U/ml青霉素)37℃、5%CO2的条件下培养至接近融合,然后在含有从水果中提取出的anthocyanin的F12K培养基中添加或不添加100ng/mlLPS,继续培养18小时,测定细胞生存率,并将培养液离心,收集上清液,用STAT-Prostaglandin E2 EIA Kit试剂盒测定培养基上清液中的PGE2浓度.结果当CRL-2606细胞暴露于300μg/ml或以上的Chokeberry提取物中时,细胞的生存率低于60%,且表现出一定的剂量效应关系.而50～400μg/ml的蓝莓提取物和100～700μg/ml的黑醋栗提取物未显示细胞毒性.LPS刺激可使细胞PEG2释放量增加一倍以上,蓝莓提取物可明显抑制由于LPS刺激引起的PGE2释放增加,100μg/ml的蓝莓提取物可完全抑制此作用而使其含量维持于正常水平.700μg/ml的黑醋栗提取物对处于LPS刺激下的CRL-2606细胞具有一定的抑制PGE2释放作用.而对于处于LPS刺激下的细胞,500μg/ml以上的Chokeberry提取物能抑制PGE2的释放.结论富含anthocyanin的黑醋栗、蓝莓以及Chokeberry提取物可以抑制由LPS刺激引起的血管内皮细胞释放PEG2增加的作用,具有一定的抗炎、抗氧化作用.

关键词：内皮细胞 PGE2 anthocyanin

MdGSTF6,activated by MdMYB1,plays an essential role in anthocyanin accumulation in apple

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Horticulture Research 2019年第1期6卷 1334-1347页

作者： Shenghui Jiang Min Chen Naibo He Xiaoliu Chen Nan Wang Qingguo Sun Tianliang Zhang Haifeng Xu Hongcheng Fang Yicheng Wang Zongying Zhang Shujing Wu Xuesen Chen College of Horticulture Science and Engineering State Key Laboratory of Crop BiologyCollaborative Innovation Center of Fruit&Vegetable Quality and Efficient Production in ShandongShandong Agricultural University61 Daizong RoadTai’an 271018China National Oceanographic Center 88 Xuzhou RoadQingdao 266071China

anthocyanins are biosynthesized on the cytosolic surface of the endoplasmic reticulum and then transported into the vacuole for storage.Glutathione S-transferases(GSTs)are considered to be responsible for the transport of anthocyanins into the vacuole.However,the regulatory mechanisms of GSTs in plants are still unclear.Here,we performed a genome-wide analysis and identified 69 GST genes in apple.The expression of MdGSTF6 was positively correlated with the anthocyanin content(r=0.949)during‘Yanfu 8’fruit development.The overexpression of MdGSTF6 in the Arabidopsis thaliana tt19 mutant resulted in seedlings of 35S::MdGSTF6-GFP/tt19 that could accumulate anthocyanin and rescue its phenotype,suggesting that MdGSTF6 was an anthocyanin transporter.The silencing of MdGSTF6 affected anthocyanin accumulation in apple fruit.Moreover,the knockdown of MdGSTF6 by RNA interference in cultured‘Gala’seedlings inhibited anthocyanin accumulation.The interaction experiments showed that MdMYB1 could bind directly to the MdGSTF6 promoter to transcriptionally activate its expression.Collectively,our results demonstrate that MdGSTF6 encodes an important GST transporter of anthocyanins in apple fruit and provide evidence for the associated regulatory mechanisms.Therefore,MdMYB1 can not only regulate anthocyanin synthesis,but also control the transport of anthocyanin in apples.This information may be useful for further clarifying the regulation of anthocyanin transport in apple.

关键词： expression anthocyanin transport

SlBBX20 interacts with the COP9 signalosome subunit SlCSN5-2 to regulate anthocyanin biosynthesis by activating SlDFR expression in tomato

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Horticulture Research 2021年第1期8卷 2121-2132页

作者： Dan Luo Cheng Xiong Aihua Lin Chunli Zhang Wenhui Sun Junhong Zhang Changxian Yang Yongen Lu Hanxia Li Zhibiao Ye Ping He Taotao Wang Key Laboratory of Horticulture Plant Biology Ministry of EducationHuazhong Agriculture University430070 WuhanChina Department of Biochemistry and Biophysics Texas A&M UniversityCollege StationTX 77843USA

anthocyanins play vital roles in plant stress tolerance and growth regulation.Previously,we reported that the photomorphogenesis-related transcription factor SlBBX20 regulates anthocyanin accumulation in tomato.However,the underlying mechanism remains unclear.Here,we showed that SlBBX20 promotes anthocyanin biosynthesis by binding the promoter of the anthocyanin biosynthesis gene SlDFR,suggesting that SlBBX20 directly activates anthocyanin biosynthesis genes.Furthermore,we found by yeast two-hybrid screening that SlBBX20 interacts with the COP9 signalosome subunit SlCSN5-2,and the interaction was confirmed by bimolecular fluorescence complementation and coimmunoprecipitation assays.SlCSN5 gene silencing led to anthocyanin hyperaccumulation in the transgenic tomato calli and shoots,and SlCSN5-2 overexpression decreased anthocyanin accumulation,suggesting thSlCSN5-2 enhanced the ubiquitination of SlBBX20 and promoted the degradation of SlBBX20 in vivo.Consistently,silencing the SlCSN5-2 homolog in tobacco significantly increased the accumulation of the SlBBX20 protein.Since SlBBX20 is a vital regulator of photomorphogenesis,the SlBBX20-SlCSN5-2 module may represent a novel regulatory pathway in light-induced anthocyanin biosynthesis.

关键词： some anthocyanin vital

PbGA2ox8 induces vascular-related anthocyanin accumulation and contributes to red stripe formation on pear fruit

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Horticulture Research 2019年第1期6卷 18-30页

作者： Rui Zhai Zhigang Wang Chengquan Yang Kui Lin-Wang Richard Espley Jianlong Liu Xieyu Li Zhongying Wu Pengmin Li Qingmei Guan Fengwang Ma Lingfei Xu College of Horticulture Northwest A&F UniversityTaicheng Road No.3YanglingShaanxi ProvinceChina The New Zealand Institute for Plant&Food Research Ltd.(Plant and Food Research)Mt.Albert Research CentrePrivate Bag92169 AucklandNew Zealand Horticultural Research Institute Henan Academy of Agricultural SciencesHuayuan Road No.116ZhengzhouHenan ProvinceChina State Key Laboratory of Crop Stress Biology for Arid Areas Northwest A&F UniversityTaicheng Road No.3YanglingShaanxi ProvinceChina

Fruit with stripes,which are generally longitudinal,can occur naturally,but the bioprocesses underlying this phenomenon are unclear.Previously,we observed an atypical anthocyanin distribution that caused red-striped fruit on the spontaneous pear bud sport“Red Zaosu”(Pyrus bretschneideri Rehd.).In this study,comparative transcriptome analysis of the sport and wild-type“Zaosu”revealed that this atypical anthocyanin accumulation was tightly correlated with abnormal overexpression of the gene-encoding gibberellin(GA)2-beta-dioxygenase 8,PbGA2ox8.Consistently,decreased methylation was also observed in the promoter region of PbGA2ox8 from“Red Zaosu”compared with“Zaosu”.Moreover,the GA levels in“Red Zaosu”seedlings were lower than those in“Zaosu”seedlings,and the application of exogenous GA4 reduced abnormal anthocyanin accumulation in“Red Zaosu”.Transient overexpression of PbGA2ox8 reduced the GA4 level and caused anthocyanin accumulation in pear fruit skin.Moreover,the presence of red stripes indicated anthocyanin accumulation in the hypanthial epidermal layer near vascular branches(VBs)in“Red Zaosu”.Transient overexpression of PbGA2ox8 resulting from vacuum infiltration induced anthocyanin accumulation preferentially in calcium-enriched areas near the vascular bundles in pear leaves.We propose a fruit-striping mechanism,in which the abnormal overexpression of PbGA2ox8 in“Red Zaosu”induces the formation of a longitudinal array of anthocyanin stripes near vascular bundles in fruit.

关键词： anthocyanin fruit abnormal