The Strawberry Fruit Fra a Allergen Functions in Flavonoid Biosynthesis
The Strawberry Fruit Fra a Allergen Functions in Flavonoid Biosynthesis作者机构：Departamento de Biologia Molecular y Bioquimica Universidad de Malaga 29071 Malaga Spain Biomolecular Food Technology Technische Universit~t M0nchen Hochfeldweg 1 85354 Freising Germany
出 版 物：《Molecular Plant》 (分子植物（英文版）)
年 卷 期：2010年第3卷第1期
页 面：113-124页
核心收录：
学科分类：0710[理学-生物学] 071001[理学-植物学] 0832[工学-食品科学与工程（可授工学、农学学位）] 07[理学] 08[工学] 083203[工学-农产品加工及贮藏工程]
基 金：DAAD
主 题：Fragaria flavonoid anthocyanin phenylpropanoid allergen pathogenesis-related proteins.
摘 要：The strawberry Fra a I allergen is a homolog of the major birch pollen allergen Bet v 1. It is synthesized by red ripe fruits of Fragaria x ananassa while white fruits of a mutant genotype, which is known to be tolerated by individuals affected by allergy, are devoid of it. Proteomic analyses have shown that Fra a 1 is down-regulated in the tolerated white- fruited genotype along with enzymes of the anthocyanin pigment pathway. In this study, we report the spatial and tem- poral expression of three Fra a genes that encode different isoforms, and the transient RNAi-mediated silencing of the Fra a genes in strawberry fruits of the red-fruited cultivar Elsanta with an ihpRNA construct. As a consequence of reduced levels of Fra a mRNAs, fruits were obtained that produced significantly decreased levels of anthocyanins and upstream metabolites. This effect is consistent with the parallel down-regulation of the phenylalanine ammonia lyase （FaPAL） and to a lesser extent of the chalcone synthase （FaCHS） transcript levels also found in these fruits. In naturally occurring white- fruited genotypes of F. chiloensis and F. vesca, Fra a transcript levels are higher than those of the red-fruited varieties, likely to compensate for the low expression levels of FaPAL and FaCHS in these mutant genotypes. The results demonstrate that Fra a expression is directly linked to flavonoid biosynthesis and show that the Fra a allergen has an essential biological function in pigment formation in strawberry fruit.
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