检索结果-南通市图书馆

全国农业生物化学与分子生物学第十五届学术研讨会

作者： Xiang-Ping Yu Wei-Chao Su Qin Wang Jiang-Xing Zhuang Rui-Qi Tong Qiong-Hua Chenc Qing-Xi Chen State Key Laboratory of Cellular Stress Biology School of Life SciencesXiamen University

Methods:The reaction media of enzyme activity assay contained 0.5 mM L-DOPA and 100μL inhibitors dissolved in 3.3%*** density at 475 nm(ε=3700 Mcm)was checked at each concentration of inhibitors using a Beckman DU800 *** were performed in 50 mM sodium phosphate buffer(pH 6.8).Assays in intrinsic fluorescence measurement contained 2.0 mL mixture with tyrosinase and *** fluorescence intensities were recorded using a Varian Cary

关键词： cardanol triene tyrosinase inhibitor fluorescence quenching molecular docking

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Screening of tyrosinase inhibitors in Prunella vulgaris using the enzyme immobilized on magnetic beads as solid-phase extraction adsorbents

Screening of tyrosinase inhibitors in Prunella vulgaris usin...

引用

第十一届全国化学生物学学术会议

作者： Xiao-Lin Bai Li Yuan Gui-Fang Wu Xun Liao Chengdu Institute of Biology Chinese Academy of Sciences

tyrosinase is the key enzyme for synthesis of melanin,and has been recognized as the target for skin whitening [1].The tyrosinase inhibitors have been used to treat some dermatosis caused by pigmentation disorders such as freckle,age pigment and so *** Chinese medicinal plants are a rich pool of bioactive compounds,in which tyrosinase inhibitors definitely *** vulgaris appears in lots of Chinse patents for skin whitening,and it is reported that flavonoids in its extract exhibited tyrosinase inhibitory effect [2].To further investigate the constituents responsible for the activity of this plant,we immobilized tyrosinase onto a carboxyl modified magnetic beads and by using it as solid-phase extraction adsorbent,to extract the binders of the enzyme from the plant’s *** components,i.e.,rosmarinic acid and caffeic acid were extracted and identified with HPLC-MS/*** kinetic assay of TYR with the two binders revealed that rosmarinic acid is the inhibitor of tyrosinase while caffeic acid the agonist of the enzyme.

关键词： tyrosinase inhibitor magnetic beads SPE Prunella vulgaris

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Inhibition of tyrosinase activity and melanine pigmentation by 2-hydroxytyrosol

引用

Acta Pharmaceutica Sinica B 2014年第2期4卷 141-145页

作者： Ryuji Uchida Seiko Ishikawa Hiroshi Tomoda Graduate School of Pharmaceutical Sciences Kitasato UniversityTokyo 108-8641Japan School of Pharmacy Kitasato UniversityTokyo 108-8641Japan

2-Hydroxytyrosol(2-HT),originally reported as a synthetic compound,was isolated for the first time as a fungal metabolite.2-HT was found to inhibit mushroom tyrosinase with an IC_(50) value of 13.0 mmol/***,2-HT dose-dependently inhibited tyrosinase activity(IC_(50),32.5 mmol/L)in the cell-free extract of B16 melanoma cells andα-melanocyte stimulating hormone(α-MSH)-stimulated melanin formation in intact B16 melanoma cells.

关键词： 2-Hydroxytyrosol Metarhizium sp. tyrosinase inhibitor Melanine formation B16 melanoma cells

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Antimelanogenic effects of Inula britannica flower petal extract fermented by Lactobacillus plantarum KCCM 11613P

引用

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2017年第9期18卷 816-824页

作者： Eun-hye PARK Won-young BAE Jae-yeon KIM Kee-tae KIM Hyun-dong PAIK Department of Food Science and Biotechnology of Animal Resources Konkuk University Bio/Molecular Informatics Center Konkuk University

The inhibitory effects of Lactobacillus plantarum-fermented and non-fermented Inula britannica extracts on the tyrosinase activity were comparatively investigated to examine whether and how they improve the whitening activity, and the contents of total flavonoids and polyphenolics as bioactive compounds were determined. The skin whitening activity using in vitro or ex vivo tyrosinase and L-3,4-dihydroxyphenylalanine(L-DOPA) staining was examined. The total flavonoid content(TFC) was increased by 13.4% after 72h-fermentation. The viabilities of the B16F10 cells treated with the fermented and non-fermented control extracts were 100.26% and 92.15% at 500μg/ml, respectively. In addition, the inhibition of tyrosinase activity was increased by the fermented samples from 29.33% to 41.74% following fermentation for up to 72h. The tyrosinase activity of the untreated control group was increased to 145.69% in B16F10 cells. The results showed that I. britannica fermented by L. plantarum dose-dependently inhibited tyrosinase activity, which was stimulated by α-melanocyte stimulating hormone. These results suggest that lactic fermented I. britannica extracts can be used as effective skin-whitening materials.

关键词： Inula britannica Flavonoid Lactobacillus plantarum Antioxidant tyrosinase inhibitor

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

不同来源的芦荟对酪氨酸酶的抑制作用(英文)

引用

中国天然药物 2013年第6期11卷 616-620页

作者： S.Dutta Gupta S.K.Masakapalli Department of Agricultural and Food Engineering Indian Institute of Technology Kharagpur Department of Plant Sciences University of Oxford

In this study, lyophilized and methanolic extracts of aloe gel from different germplasms were evaluated for their potential to inhibit mushroom tyrosinase activity. The results showed potent inhibitory effect of Aloe vera gel extracts on L-dihydroxyphenylalanine(L-DOPA) oxidation catalyzed by tyrosinase in a dose-dependent manner. Significant differences in % inhibition of tyrosinase among the extraction methods and the germplasms were observed. The relative performance of the germplasms was evaluated with the help of posthoc multicomparison test. The methanolic extract was more effective than the lyophilized crude gel in all the germplasms. The inhibitory effect of the lyophilized gel and methanolic extract tested from five germplasms followed the order: RM > TN > S24 > OR > RJN. The germplasm RM showed the highest tyrosinase inhibition, and the maximum % inhibition noted was 26.04% and 41.18%, respectively for the lyophilized and methanolic extracts at 6 mg·mL–1concentration. Lineweaver-Burk plots of the different concentrations of L-DOPA in the absence and presence of lyophilized gel extract showed competitive inhibition of mushroom tyrosinase in all the germplasms. This study suggests that the germplasm RM could potentially be used for the isolation and identification of the effective tyrosinase inhibitory component, and ascertains the critical role of selecting the best source of germplasm for natural product isolation and characterization.

关键词： Aloe vera L.gel tyrosinase inhibitor L-DOPA Inhibition kinetics Germplasm Sourcing

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Ferulic Acid in Paper Mulberry and Its Wastewater from Pulps Production

引用

Journal of Chemistry and Chemical Engineering 2010年第7期4卷 58-62页

作者： Mayuree Kanlayavattanakul Nattaya Lourith School of Cosmetic Science Mae Fah Luang University Chiang Rai 57100 Thailand

Wastewater from paper mulberry （Broussonetia papyrifera） pulps production was subjected to biological activities evaluations and phenolics characterization. The EtOAc extract was found to be the best antioxidant （IC50 = 0.4408 mg/mL） and tyrosinase inhibitor （IC50 = 1.6340 mg/mL） comparing to vitamin C （1C50 = 0.0011 mg/mL） and kojic acid （IC50 = 0.0244 mg/mL）, respectively, and the highest ferulic acid content （4.450 ±0.0077 ppm）. This fraction was further column chromatographed and antioxidant activity was archived （IC50 = 0.075 mg/mL） as well as tyrosinase inhibitory effect （IC50 = 0.042 mg/mL） in addition to ferulic acid （45.310 ± 0.0115 ppm）. Ferulic acid in paper mulberry was compared. Reflux with H2O gave the highest ferulic acid content （37.848 ± 1.2470 ppm） followed by a mixture of MeOH and H2O （2.002 ± 0.0370 ppm） and MeOH （1.185 ± 0.0320 ppm）, respectively. The reflux H2O was partitioned with EtOAc that extracted most of ferulic acid （256.678 ± 0.3760 ppm） and left less in the aqueous layer （9.408 ± 0.2820 ppm）.

关键词： Paper mulberry broussonetia papyrifera wastewater tyrosinase inhibitor DPPH-radical scavenging activity.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Synthesis,structure and tyrosinase inhibition of natural phenols derivatives

引用

Journal of Chinese Pharmaceutical Sciences 2011年第3期20卷 235-244页

作者：王艳丽黄伟陈帅陈石泉王世范海南大学海洋学院制药工程系海南海口570228 海南大学海洋生物实验教学中心海南海口570228

Four series of derivatives of vanillin,anisaldehyde,genistein and aloe emodin were *** single crystal of （E）-1-（5-（4-methoxyphenyl）-3-（4-methoxystyryl）-4,5-dihydro-1H-pyrazol-1-yl）ethanone,which was synthesized from vanillin, was obtained by spontaneous evaporation *** the compounds were tested for the tyrosinase inhibitory *** results demonstrated that the IC_（50）values of vanillin,anisaldehyde,genistein and aloe emodin against mushroom tyrosinase activity are 68,49,343 and 160μM,*** the twelve derivatives,（E）-1-（5-（4-methoxyphenyl）-3-（4-methoxystyryl）-4,5- dihydro-1H-pyrazol-1-yl）ethanone from vanillin and 4,5-dimethoxy-9,10-dioxo-9,10-dihydroanthracene-2-carboxylic acid from aloe emodin showed good inhibition against the tyrosinase with IC_（50）values of 18μM and 21μM,respectively.

关键词： Natural phenols Derivatives Synthesis tyrosinase inhibitor

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Design,Synthesis,and Fungicidal Activity of Substituted Cinnamaldehyde Thiosemicarbazone inhibitors on tyrosinase

Design,Synthesis,and Fungicidal Activity of Substituted Cinn...

引用

第十届全国化学生物学学术会议

作者： Xuebo Zhang Xinling Yang Peng Lei Xiaohe Zhang Yun Ling Department of Applied Chemistry College of ScienceChina Agricultural University

Melanin has the significant physiological function not only in the growth of insect but also in fungi and bacteria. tyrosinase is the key enzyme in the formation of melanin, which distributed in animals, plants and microbes. More than ten tyrosinase crystal structures had been found since the crystal structure of tyrosinase from *** was first obtained by multiple homo-crystal substitutions in 2006. Therefore, inhibiting the activity of tyrosinase or controlling the biosythesis of melanin is a potent way to find new agrochemicals. In our previous work, PMDD-5 Y, one of thiosemicarbazone analogues, not only has tyrosinase inhibitory activity but also has antifungal activity. However, its low solubility in organic and inorganic solvent is not favorable for pesticide formulations and transmission. As a hydrophobic fragment, piperidine plays an important role in pesticide and medicine industry. Piperidine derivatives have been studies extensively by organic chemists due to their various biological properties. In the present work, in order to improve the water-solubility or fat-solubility of PMDD-5 Y, a series of thiosemicarbazone analogues containing piperidine moiety were designed and synthesized via linking activity sub-structures method based on the lead compound PMDD-5 Y（Figure 1）, their tyrosinase inhibiting activity and fungicidal activities were also studied.

关键词： Thiosemicarbazide tyrosinase inhibitor Piperidine derivatives Fungicidal activity

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：