Inhibitory mechanism of cardanol triene as a novel tyrosinase inhibitor
作者单位：State Key Laboratory of Cellular Stress BiologySchool of Life SciencesXiamen University
会议名称：《全国农业生物化学与分子生物学第十五届学术研讨会》
会议日期：2016年
学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术]
基 金：supported by the Natural Science Foundation of China(Grant No.31570785 and No.J1310027)
关 键 词：cardanol triene tyrosinase inhibitor fluorescence quenching molecular docking
摘 要：Methods:The reaction media of enzyme activity assay contained 0.5 mM L-DOPA and 100μL inhibitors dissolved in 3.3%*** density at 475 nm(ε=3700 Mcm)was checked at each concentration of inhibitors using a Beckman DU800 *** were performed in 50 mM sodium phosphate buffer(pH 6.8).Assays in intrinsic fluorescence measurement contained 2.0 mL mixture with tyrosinase and *** fluorescence intensities were recorded using a Varian Cary