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Influence of Shenqing Recipe on Morphology and Quantity of Colonic Interstitial Cells of Cajal in trinitrobenzene sulfonic acid Induced Rat Colitis

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Chinese Medical Sciences Journal 2011年第1期26卷 43-48页

作者： Yan-cheng Dai Zhi-peng Tang Zhen-nan Wang Ya-li Zhang Xin-ying He Department of Gastroenterology Longhua HospitalInstitute of Digestive DiseaseShanghai University of Traditional Chinese MedicineShanghai 200032China

Objective To observe the influence of Shenqing Recipe (SQR), a kind of Traditional Chinese Medicine, on the morphology and quantity of colonic interstitial ceils of Cajal (ICC) in trinitrobenzene sulfonic acid (TNBS)-induced rat colitis, and to investigate the possible mechanism of SQR in regulating intestinal dynamics. Methods Sixty rats were randomly divided into normal control, model Ⅰ , model Ⅱ, mesalazine, and high-dose, and low-dose SQR groups with 10 rats in each group. TNBS (10 mg) dissolved in 50% ethanol was instilled into the lumen of the rat colon of the latter five groups to induce colitis. On the 4th day after administration of TNBS, each treatment group was administered one of the following formulations by enteroclysis gavage once a day for 7 days: 600 mg·kg^-1·d^-1 mesalazine, 2.4g^-1·d^-1 SQR, and 1.2g^-1·d^-1 SQR. Model Ⅱ rats received normal saline solution. After 7 days colonic samples were collected. While the colonic samples of model I group were collected on the 3rd day after TNBS administered. Ultrastructure of ICC in the damaged colonic tissues was observed with transmission electron microscope. Expression of c-kit protein in colonic tissue was determined by immunohistochemical staining and Western blot. Results The ultrastructure of colonic ICC in the rat model of TNBS-induced colitis showed a severe injury, and administration of SQR or mesalazine reduced the severity of injury. Similarly, the expression of c-kit protein of TNBS-induced colitis rat model was significantly decreased compared with the normal control group (P〈0.05). Treatment with SQR or mesalazine significantly increased the expression of c-kit protein compared with the administration of control formulations (P〈0.05), especially the high-dose SQR group. Conclusion SQR could alleviate and repair the injured ICC, and improve its quantity, which might be involved in regulating intestinal motility.

关键词： Shenqing Recipe trinitrobenzene sulfonic acid colitis c-kit interstitialcells of Cajal

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Key factors in developing the trinitrobenzene sulfonic acid-induced post-inflammatory irritable bowel syndrome model in rats

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World Journal of Gastroenterology 2012年第20期18卷 2481-2492页

作者： Hong-Yan Qin Hai-Tao Xiao Justin CY Wu Brian M Berman Joseph JY Sung Zhao-Xiang Bian School of Chinese Medicine Hong Kong Baptist University Hong Kong 999077 China Faculty of Medicine The Chinese University of Hong Kong.Hong Kong 999077 China Center for Integrative Medicine University of Maryland School of Medicine Baltimore MD 21201 United States

AIM:To investigate the key factors in developing the trinitrobenzene sulfonic acid(TNBS)-induced postinflammatory irritable bowel syndrome(PI-IBS)model in rats. METHODS:TNBS was administered to rats at the following conditions:(1)with different doses(20,10,5 mg/0.8 mL per rat);(2)with same dose in different concentrations(20 mg/rat,25,50 mg/mL);(3)in different ethanol percentage(25%,50%);and(4)at depth either 4 cm or 8 cm from *** 5 d and 4 wk after TNBS administration,inflammation severity and inflammation resolution were *** 4 and 8 wk after TNBS application,visceral hyperalgesia and enterochromaffin(EC)cell hyperplasia were assayed by abdominal withdrawal reflex test,silver staining and capillary electrophoresis. RESULTS:Our results showed that:(1)TNBS induced dose-dependent acute inflammation and inflammation *** 5 d post TNBS,the pathological score and myeloperoxidase(MPO)activity in all TNBS treated rats were significantly elevated compared to that of the control(9.48±1.86,8.18±0.67,5.78± 0.77 vs 0,and 3.55±1.11,1.80±0.82,0.97±0.08 unit/mg vs 0.14±0.01 unit/mg,P<0.05).At 4 wk post TNBS,the pathological score in high and median dose TNBS-treated rats were still significantly higher than that of the control(1.52±0.38 and 0.80±0.35 vs 0,P<0.05);(2)Intracolonic TNBS administration position affected the persistence of visceral *** 4 wk post TNBS,abdominal withdrawal reflex (AWR)threshold pressure in all TNBS-treated groups were decreased compared to that of the control(21.52 ±1.73 and 27.10±1.94 mmHg vs 34.44±1.89 mmHg,P<0.05).At 8 wk post TNBS,AWR threshold pressure in 8 cm administration group was still significantly decreased(23.33±1.33 mmHg vs 36.79±2.29 mmHg,P<0.05);(3)Ethanol percentage affected the TNBS-induced inflammation severity and visceral *** TNBS-25%ethanol-treated group,the pathological score and MPO activity were significantly lowered compared to that of the TNBS-50%ethanoltreated group,while AWR thres

关键词： Post-inflammatory Irritable bowel syndrome Rat model trinitrobenzene sulfonic acid Key factors

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The Modulatory Effect of Dietary Apostichopus japonicus on Mice with Ulcerative Colitis Induced by trinitrobenzene sulfonic acid

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Journal of Biosciences and Medicines 2016年第10期4卷 15-27页

作者： Hongjie Shi Hong Sun Rong Zheng Shangyun Lu Fang Liu Na Zhang Changhu Xue Qingjuan Tang College of Food Science and Engineering Ocean University of China Qingdao China Emergency Medicine Shandong Jining No. 2 Peoples Hospital Jining China

Sea cucumber is a food with nutritional benefits distributing mainly in Asia, and Apostichopus japonicus (A. japonicus) is a kind of sea cucumber whose quality is better than any others. However, different processing methods make various effect on its quality. In this study, we evaluated the protection effect of A. japonicus with different processing methods on mice with ulcerative colitis induced by trinitrobenzene sulfonic acid (TNBS), especially on the intestinal microflora. The expression of IFN-γ/IL-4 and IL-1β in gut, and intestinal microbiota were discussed. The results revealed that three different processing methods of A. japonicus could decrease the expression of inflammatory cytokines, except for the expression of IFN-γ/IL-4 treated with enzymatic, and dried A. japonicus was the most efficient. A. japonicus could change the microbiotic imblance relatively back to normal in terms of bacterial diversity and composition, meanwhile increase the abundance of Bifidobacteria, Lactobacillus and Clostridium leptum. The elements of protein, polysaccharide in dried, instant, enzymatic A. japonicus are 73.09%, 65.06%, 57.42% and 6.72%, 5.46%, 5.45% respectively. This study indicated that A. japonicus have a good improving effect on ulcerative colitis, especially on the microbiome, and processing methods had an effect on alleviation of ulcerative colitis, which might be associated with content of protein and polysaccharide.

关键词： Apostichopus japonicus Ulcerative Colitis trinitrobenzene sulfonic acid Intestinal Microbiota

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Anti-inflammatory efficiency of levobupivacaine in an experimental colitis model

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World Journal of Gastroenterology 2010年第20期16卷 2537-2541页

作者： Ugur Duman Aysun Yilmazlar Ersin Ozturk Sibel Aker Emre Sarandol Tuncay Yilmazlar Department of General Surgery Uludag University School of Medicine16069 BursaTurkey Department of Anesthesiology and Reanimation Uludag University School of Medicine16069 BursaTurkey Department of Pathology Uludag University School of Medicine16069 BursaTurkey Department of Biochemistry Uludag University School of Medicine16069 BursaTurkey

AIM:To investigate the efficiency of levobupivacaine in treating experimentally induced colitis in ***:Colitis was induced by trinitrobenzene sulfonic acid and ethanol in 30 rats under general anesthesia,and 10 rats were used as a sham *** to induction of colitis,rats were divided into three groups;budesonide group received 0.1 mg/kg budesonide,levobupivacaine group received 10 mg/kg levobupivacaine and saline group received 1 mL saline solution via rectal route for 7 *** the sham group,only routine rectal catheterization was performed without use of any *** the end of 7 d,laparotomy and total colectomy were performed for histopathological ex-amination in all rats and blood samples were drawn for measurement of tumor necrosis factor(TNF)-αand interleukin(IL)-6 following cardiac *** and microscopic evaluations of the specimens were performed by a pathologist blinded to group assignment of the ***:Weight loss(P=0.016)and macroscopic examination scores(P=0.001)were significantly higher in saline group than *** scoring was comparable between all colitis groups(P=0.350).There was no significant difference in TNF-αlevels and IL-6 levels(P=0.150).CONCLUSION:The significant improvement in macroscopic scores suggests that levobupivacaine may have topical anti-inflammatory effects in an experimental colitis model;however,this finding was not supported by microscopic findings.

关键词： trinitrobenzene sulfonic acid Colitis Levobupivacaine Budesonide

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Effect of homocysteine on intestinal permeability in rats with experimental colitis,and its mechanism

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Gastroenterology Report 2014年第3期2卷 215-220页

作者： Hao Ding Qiao Mei Hui-Zhong Gan Li-Yu Cao Xiao-Chang Liu Jian-Ming Xu Department of Gastroenterology The First Affiliated Hospital of Anhui Medical UniversityThe Key Laboratory of Digestive Diseases of Anhui ProvinceHefeiChina Department of Pathology The First Affiliated Hospital of Anhui Medical UniversityHefeiChina

Objective:To investigate the effect of homocysteine(Hcy)on intestinal permeability in rats with TNBS/ethanol-induced colitis and elucidate its ***:Sprague-Dawley rats were divided into four groups:normal,normal+Hcy injection,TNBS model,and TNBS model+Hcy *** colitis was induced by trinitrobenzene sulfonic acid(TNBS)in 50%ethanol;rats were injected subcutaneously with Hcy from the first day after the induction of experimental colitis on 30 consecutive *** determine the severity of colitis,the disease activity index(DAI)was evaluated;colon tissues were collected for the detection of the activity of myeloperoxidase(MPO)and the contents of MDA,IL-1β,IL-6,TNF-α,MMP-2,and *** epithelial permeability was assessed with Evans blue(EB)*** levels of Hcy in plasma and colon mucosa were measured by high-performance liquid chromatography-fluorescence detection(HPLC-FD).Results:Compared with the normal group,the DAI scoring and MPO activity,contents of MDA,IL-1β,IL-6,TNF-α,MMP-2,and MMP-9in the colon and EB in the small intestine were significantly increased in the TNBS group(P<0.01).Compared with the TNBS model group,the DAI scoring,plasma and colonic mucosa Hcy levels,MPO activity and contents of MDA,IL-1β,IL-6,TNF-α,MMP-2,and MMP-9 in colon and EB in small intestine were significantly increased in the TNBS-induced colitis rats with simultaneous Hcy injection(P<0.01).Conclusion:Hcy can increase intestinal permeability and aggravate inflammatory damage in rats with TNBS-induced colitis,the underlying mechanisms of which may be attributed to its effects of promoting the expression of MMP-2 and MMP-9,leading to injury of the intestinal barrier.

关键词： homocysteine colitis trinitrobenzene sulfonic acid intestinal permeability

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Effect of Baicalin on TNBS-Induced Colonic Inflammatory Injury in Rats

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Health 2023年第9期15卷 938-943页

作者： Yu Feng Min Guo Department of Gastroenterology The Fourth People’s Hospital of Haikou Haikou China

Objective: The aim is to observe the protective effect of baicalin on trinitrobenzene sulfonic acid (TNBS)-induced experimental colitis in mice and explore its mechanism. Methods: The mice were divided into 4 groups: ethanol control group, TNBS model group, baicalin low-dose group and baicalin high-dose group. The model of experimental colitis in mice was induced by TNBS enema. After 2 hours of TNBS enema, baicalin was given by gavage, QD × 7D. The animals were sacrificed on the 8th day to observe the extent of colonic mucosal damage, and the Peroxidase activity, Malondialdehyde (MDA) and glutathione (GSH) contents were measured. Results: Compared with the TNBS model group, the body weight, gross injury score and histological changes were significantly improved;MPO enzyme activity and MDA content were significantly decreased in the low and high-dose baicalin groups;and the content of glutathione increased. Conclusion: Baicalin can alleviate TNBS-induced colitis in mice, and the mechanism is related to the antioxidation of baicalin.

关键词： Baicalin trinitrobenzene sulfonic acid Experimental Colitis Mouse

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Role of β2-Adrenoceptor-β-Arrestin2-Nuclear Factor- k B Signal Transduction Pathway and Intervention Effects of Oxymatrine in Ulcerative Colitis

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Chinese Journal of Integrative Medicine 2012年第7期18卷 514-521页

作者：范恒廖奕唐庆陈小艳张丽娟刘星星钟敏 Department of Integrated Chinese and Western Medicine Union HospitalTongji Medical CollegeHuazhong University of Science and Tecnology

Objective： To investigate the β2-adrenoceptor （ β 2AR）-β-arrestin2-nuclear factor- K B （NF- κ B） signal transduction pathway and the intervention effects of oxymatrine in a rat model of ulcerative colitis. Methods： Forty SD rats were randomly divided into four groups, which included the normal control group, the model group, the mesalazine group and the oxymatrine treatment group, with 10 rats per group. Experimental colitis induced with trinitrobenzene sulfonic acid （TNBS） was established in each group except the normal control group, The rats in the oxymatrine treatment group were treated with intramuscular injection of oxymatrine 63 mg/（kg.d） for 15 days and the rats in the mesalazine group were treated with mesalazine solution 0.5 g/（kg.d） by gastric lavage for 15 days. The rats in the normal control group and model group were treated with 3 mL water by gastric lavage for 15 days. Diarrhea and bloody stool were carefully observed. Histological changes in colonic tissue were examined on day 7 in 2 rats per group that were randomly selected. The expression of β 2AR, β -arrestin2 and NF- κ B p65 in colon tissue and spleen lymphocytes were detected with immunohistochemistry and Western immunoblotting techniques on day 16 after fasting for 24 h. Six rats died of lavage with 2 each in the normal control, the model group and the mesalazine group; and were not included in the analysis. Results： The rats in the model group suffered from looser stool and bloody purulent stool after modeling. But in the oxymatrine and mesalazine groups, looser stool and bloody purulent stool reduced after treatment. And the colonic wall in the model group was thickened and the colon length shortened. The colon mucosa was congested in multiple areas with edema, erosion, superficial or linear ulcer and scar formation, while the intestinal mucosa injury reduced in the mesalazine and oxymatrine groups （P〈0.01）. In colonic mucosa and in spleen lymphocytes, compared with the normal control group

关键词： β 2-adrenoceptor β-arrestin2 nuclear factor- κB signal transduction trinitrobenzene sulfonic acid ulcerative colitis oxymatdne

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