Effect of homocysteine on intestinal permeability in rats with experimental colitis,and its mechanism

作     者：Hao Ding Qiao Mei Hui-Zhong Gan Li-Yu Cao Xiao-Chang Liu Jian-Ming Xu 

作者机构：Department of GastroenterologyThe First Affiliated Hospital of Anhui Medical UniversityThe Key Laboratory of Digestive Diseases of Anhui ProvinceHefeiChina Department of PathologyThe First Affiliated Hospital of Anhui Medical UniversityHefeiChina 

出 版 物：《Gastroenterology Report》 (胃肠病学报道（英文）)

年 卷 期：2014年第2卷第3期

页      面：215-220页

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：supported by The Natural Science Foundation of Anhui Province(1308085MH146) Fund of Yang Sen Science Research Council China(2012JRCC Digest 02) 

主　　题：homocysteine colitis trinitrobenzene sulfonic acid intestinal permeability 

摘      要：Objective:To investigate the effect of homocysteine(Hcy)on intestinal permeability in rats with TNBS/ethanol-induced colitis and elucidate its ***:Sprague-Dawley rats were divided into four groups:normal,normal+Hcy injection,TNBS model,and TNBS model+Hcy *** colitis was induced by trinitrobenzene sulfonic acid(TNBS)in 50%ethanol;rats were injected subcutaneously with Hcy from the first day after the induction of experimental colitis on 30 consecutive *** determine the severity of colitis,the disease activity index(DAI)was evaluated;colon tissues were collected for the detection of the activity of myeloperoxidase(MPO)and the contents of MDA,IL-1β,IL-6,TNF-α,MMP-2,and *** epithelial permeability was assessed with Evans blue(EB)*** levels of Hcy in plasma and colon mucosa were measured by high-performance liquid chromatography-fluorescence detection(HPLC-FD).Results:Compared with the normal group,the DAI scoring and MPO activity,contents of MDA,IL-1β,IL-6,TNF-α,MMP-2,and MMP-9in the colon and EB in the small intestine were significantly increased in the TNBS group(P0.01).Compared with the TNBS model group,the DAI scoring,plasma and colonic mucosa Hcy levels,MPO activity and contents of MDA,IL-1β,IL-6,TNF-α,MMP-2,and MMP-9 in colon and EB in small intestine were significantly increased in the TNBS-induced colitis rats with simultaneous Hcy injection(P0.01).Conclusion:Hcy can increase intestinal permeability and aggravate inflammatory damage in rats with TNBS-induced colitis,the underlying mechanisms of which may be attributed to its effects of promoting the expression of MMP-2 and MMP-9,leading to injury of the intestinal barrier.