Role of β2-Adrenoceptor-β-Arrestin2-Nuclear Factor- k B Signal Transduction Pathway and Intervention Effects of Oxymatrine in Ulcerative Colitis

作     者：范恒 廖奕 唐庆 陈小艳 张丽娟 刘星星 钟敏 

作者机构：Department of Integrated Chinese and Western MedicineUnion HospitalTongji Medical CollegeHuazhong University of Science and Tecnology 

出 版 物：《Chinese Journal of Integrative Medicine》 (中国结合医学杂志（英文版）)

年 卷 期：2012年第18卷第7期

页      面：514-521页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by the National Natural Science Foundation of China (No.30772878) 

主　　题：β 2-adrenoceptor β-arrestin2 nuclear factor- κB signal transduction trinitrobenzene sulfonic acid ulcerative colitis oxymatdne 

摘      要：Objective： To investigate the β2-adrenoceptor （ β 2AR）-β-arrestin2-nuclear factor- K B （NF- κ B） signal transduction pathway and the intervention effects of oxymatrine in a rat model of ulcerative colitis. Methods： Forty SD rats were randomly divided into four groups, which included the normal control group, the model group, the mesalazine group and the oxymatrine treatment group, with 10 rats per group. Experimental colitis induced with trinitrobenzene sulfonic acid （TNBS） was established in each group except the normal control group, The rats in the oxymatrine treatment group were treated with intramuscular injection of oxymatrine 63 mg/（kg.d） for 15 days and the rats in the mesalazine group were treated with mesalazine solution 0.5 g/（kg.d） by gastric lavage for 15 days. The rats in the normal control group and model group were treated with 3 mL water by gastric lavage for 15 days. Diarrhea and bloody stool were carefully observed. Histological changes in colonic tissue were examined on day 7 in 2 rats per group that were randomly selected. The expression of β 2AR, β -arrestin2 and NF- κ B p65 in colon tissue and spleen lymphocytes were detected with immunohistochemistry and Western immunoblotting techniques on day 16 after fasting for 24 h. Six rats died of lavage with 2 each in the normal control, the model group and the mesalazine group; and were not included in the analysis. Results： The rats in the model group suffered from looser stool and bloody purulent stool after modeling. But in the oxymatrine and mesalazine groups, looser stool and bloody purulent stool reduced after treatment. And the colonic wall in the model group was thickened and the colon length shortened. The colon mucosa was congested in multiple areas with edema, erosion, superficial or linear ulcer and scar formation, while the intestinal mucosa injury reduced in the mesalazine and oxymatrine groups （P〈0.01）. In colonic mucosa and in spleen lymphocytes, compared with the normal control group