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Nano Research 2024年第9期17卷 8209-8219页

作者： Zhongwei Guo Shiqiang Zhang Yilin Guo Jingjing Xia Xiao Wu Hao Hu Rongcheng Hu Fangli Huang Qiulei Gao Chun Liu Jingjiang Qiu Wei Sun School of Mechanics and Safety engineering Zhengzhou UniversityZhengzhou 450001China Department of Mechanical engineering and Mechanics Tsinghua UniversityBeijing 100084China Precision Medicine Institute Guangdong Provincial Key Laboratory of Orthopedics and TraumatologyThe First Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510080China Department of Mechanical engineering Drexel UniversityPhiladelphiaPA 19104USA

engineering hydrogels that resemble biological tissues of various lengths via conventional fabrication techniques remains ***-dimensional(3D)bioprinting has emerged as an advanced approach for constructing complex biomimetic 3D architectures,which are currently restricted by the limited number of available bioinks with high printability,biomimicry,biocompatibility,and proper mechanical *** by ubiquitous coacervation phenomena in biology,we present a unique mineral-biopolymer coacervation strategy that enables the hierarchical assembly of nanoclay and recombinant human collagen(RHC).This system was observed to undergo a coacervation transition(liquid‒liquid phase separation)*** formed dense phase separated from its supernatant is the coacervate of clay-RHC-rich complexes,where polymer chains are sandwiched between silicate *** dynamics simulation was first used to verify and explore the coacervation ***,the coacervates were demonstrated to be potential bioinks that exhibited excellent self-supporting and shear-thinning viscoelastic *** extrusion-based printing,the versatility of the bioink was demonstrated by reconstructing the key features of several biological tissues,including multilayered lattice,vascular,nose,and ear-like structures,without the need for precrosslinking operations or support ***,the printed scaffolds were cytocompatible,elicited minimal inflammatory responses,and promoted bone regeneration in calvarial defects.

关键词： bioinks coacervation mineral-biopolymer nanoclay tissue engineering scaffold

Efficient calculation of fluid-induced wall shear stress within tissue engineering scaffolds by an empirical model

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Medicine in Novel Technology and Devices 2023年第2期18卷 267-271页

作者： Husham Ahmed Matthew Bedding-Tyrrell Davide Deganello Zhidao Xia Yi Xiong Feihu Zhao Zienkiewicz Centre for Computational engineering and Department of Biomedical engineering Faculty of Science&EngineeringSwansea UniversitySwanseaSA18ENUK Welsh Centre for Printing and Coating Faculty of Science and EngineeringSwansea UniversitySwanseaSA18ENUK Centre for Nanohealth Swansea University Medical School and Faculty of MedicineHealth and Life ScienceSwansea UniversitySwanseaSA35AUUK School of System Design and Intelligent Manufacturing Southern University of Science and TechnologyShenzhen518055China

Mechanical stimulation,such as fluid-induced wall shear stress(WSS),is known that can influence the cellular ***,in some tissue engineering experiments in vitro,mechanical stimulation is applied via bioreactors to the cells in cell culturing to study cell physiology and *** 3D cell culturing,porous scaffolds are used for housing the *** is known that the scaffold porous geometries can influence the scaffold permeability and internal WSS in a bioreactor(such as perfusion bioreactor).To calculate the WSS generated on cells within scaffolds,usually computational fluid dynamics(CFD)simulation is ***,the limitations of the computational method for WSS calculation are:(i)the high time cost of the CFD simulation(in particular for the highly irregular geometries);(ii)accessibility to the CFD model for some cell culturing experimentalists due to the knowledge *** address these limitations,this study aims to develop an empirical model for calculating the WSS based on scaffold *** model can allow the tissue engineers to efficiently calculate the WSS generated within the scaffold and/or determine the bioreactor loading without performing the computational simulations.

关键词： Wall shear stress Permeability Empirical model tissue engineering scaffold Bioreactor

Characterization on Modification and Biocompatibility of PCL scaffold Prepared with Near-field Direct-writing Melt Electrospinning

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Chemical Research in Chinese Universities 2021年第3期37卷 578-583页

作者： CHEN Zhijun HAO Ming QIAN Xiaoming CHEN Wenyang ZENG Ming HUANG Juan LI Ruixin FAN Jintu LIU Yanbo School of Textile Science and engineering State Key Laboratory of Separation Membranes and Membrane ProcessesTiangong UniversityTianjin300387P.R.China School of Textile Science and engineering Key Laboratory of Textile Fiber and ProductMinistry of EducationWuhan Textile UniversityWuhan430200P.R.China Central Laboratory Tianjin300041P.R.China Tianjin Stomatological Hospital Tianjin Key Laboratory of Oral and Maxillofacial Function ReconstructionTianjin300041P.R.China

In this study,orthogonal experiments were designed to explore the optimal process parameters for preparing polycaprolactone(PCL)scaffolds by the near-field direct-writing melt electrospinning(NFDWMES)*** on the optimal process parameters,the PCL scaffolds with different thicknesses,gaps and structures were manufactured and the corresponding hydrophilicities were *** PCL scaffolds were modified by chitosan(CS)and hyaluronic acid(HA)to improve biocompatibility and *** Fourier transform infrared spectroscopy(FTIR)analysis and antibacterial experimental results show that the chitosan and hyaluronic acid adhere to the surface of PCL scaffolds,suggesting that the modification plays a positive role in biocompatibility and antibacterial *** PCL scaffolds were then employed as a carrier to culture *** morphology and distribution of the cells observed by a fluorescence microscope demonstrate that the modified PCL scaffolds have good biocompatibility,and the porous structure of the scaffolds is conducive to adhesion and deep growth of cells.

关键词： Melt electrospinning Near-field direct-writing tissue engineering scaffold Polycaprolactone Modification

Aligned Fibrous scaffold Induced Aligned Growth of Corneal Stroma Cells in vitro Culture

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Chemical Research in Chinese Universities 2012年第6期28卷 1022-1025页

作者： GAO Yan YAN Jing CUI Xue-jun WANG Hong-yan WANG Qing College of Chemistry Jilin University Changchun 130012 P. R. China China-Japan Union Hospital Jilin University Changchun 130033 P. R. China

To investigate the contribution of fibre arrangement to guiding the aligned growth of corneal stroma cells,aligned and randomly oriented fibrous scaffolds of gelatin and poly-L-lactic acid（PLLA） were fabricated by electrospinning.A comparative study of two different systems with corneal stroma cells on randomly organized and aligned fibres were *** efficiency of the scaffolds for inducing the aligned growth of cells was assessed by morphological observation and 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl-tetrazolium bromide（MTT） *** show that the cells cultured on both randomly oriented and aligned scaffolds maintained normal morphology and well spreading as well as long term ***,corneal stroma cells grew high orderly on the aligned scaffold,while the cells grew disordered on the randomly oriented ***,the cells exhibited higher viability in aligned scaffold than that in randomly oriented *** results indcate that electrospinng to prepare aligned fibrous scaffolds has provided an effective approach to the aligned growth of corneal stroma cells in *** findings that fiber arrangement plays a crucial role in guiding the aligned growth of cells may be helpful to the development of better biomaterials for tissue engineered cornea.

关键词： Electrospnning Fiber alignment Stroma cells proliferation tissue engineering scaffold

Three-dimensionally Perforated Calcium Phosphate Ceramics

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Journal of Wuhan University of Technology(Materials Science) 2005年第B12期20卷 223-225页

作者： Atsuo Ito T Sakurai Y Sogo M Ikeuchi H Ohgushi T Mutsuzaki Institute for Human Science and Biomedical engineering National Institute of Advanced Industrial Science and Technology Central 6 1-1-1 Higashi Tsukuba 305-8566 Japan Research Institute for Cell engineering National Institute of Advanced Industrial Science and TechnologyAmagasaki Site 3-11-46 Nakouji Amagasaki Hyogo 661-0974 Japan Department of Orthopedic Surgery University of Tsukuba 1-1-1 Tennodai Tsukuba 305-8571 Japan

Porous calcium phosphate ceramics were produced by compression molding using a special mold followed by sintering. The porous calcium phosphate ceramics have three-dimensional and penetrated open pores 380-400μm in diacneter spaced at intervals of 200μm. The layers of the linear penetration pores alternately lay perpendicular to pore direction. The porosity was 59%-65% . The Ca/ P molar ratios of the porous calcium phos phate ceramics range from 1.5 to 1.85. A binder cantaining methyl cellulose was most effective for preparing the powder compact among vinyl acetate, polyvinyl alcohol, starch, stearic acid, methyl cellulose and their mixtures . Stainless steel, polystyrene, nylon and bamboo were used as the long columnar dies for the penetrated open pores. When polystyrene, nylon and bamboo were used as the long columnar male dies, the dies were burned oat during the sintering process. Using stainless steel as the male dies with the removal of the dies before heat treatment resulted in a higher level of densification of the calcium phosphate ceramic.

关键词： calcium phosphate porous ceramic three-dimensionally perforated tissue engineering scaffold tricalcium phosphate

Electrospinning and Electrospun Nanofibers

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World Journal of Nano Science and engineering 2016年第2期6卷 45-50页

作者： Lefayet Sultan Lipol Md. Moshiur Rahman University of Borås Bor&arings Sweden

Electro-spinning is a very modern process which can be used in various purposes. We did this experimental work at Swerea IVF in Sweden during M. Sc in Textile Technology programme at University of Bor?s. We should especially thank our supervisor—Anna Thorvaldsson and course teacher—Ioannis S. Chronakis. In this report, we have tried to explain the basic manufacturing techniques of the electrospun nanofiber by the electro-spinning, how one can characterize it by SEM (Scanning Electron Microscopy) and its various applications in the practical field, e.g wound healing, tissue engineering scaffold. The experimental work helped us a lot to gather sufficient knowledge about the electro-spinning process which we wanted to share with all.

关键词： Nano-Fibers tissue engineering scaffold Nano-Collagen Cartilage and Bones Bladder and Kidney SEM (Scanning Electron Microscopy)

In vitro Mineralization Behavior of the Sol-gel Derived Bioglass/Collegen Composite Porous scaffold

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Journal of Wuhan University of Technology(Materials Science) 2005年第B12期20卷 22-23,34页

作者：陈晓峰 The Key Laboratory of Specially Functional Materials and Advanced Manufacturing Technology of Ministry of Education South China University of Technology Guangzhou 510640 China

The porous scaffold of the sol-gel derived bioactive glass （BG） in the system CaO-P2O5-SiO2 was treated with the type Ⅰ collagen solution. The pore walls of the scaffold were covered by the collagenous network. The in vitro mineralization behavior of the sol- gel derived bioglassl collegen composite porous scaffold was investigated by immersion in supersaturated calcification solution （ SCS ） at 37℃ for different times, XRD , FTIR, SEM/ EDAX techniques were applied to analyze the crystalline phases, morphology and composition of the minerals formed on the pore walls of the scaffold. It was found that with increasing of immersion time, the morphology of reaction products on the pore walls changed from the spherical particles of calcium phosphate to the flake-like HCA crystals.

关键词： collagen sol-gel bioactive glass tissue engineering scaffold hydroxyl-carbonateapatite ( HCA )

The Construction of Heparin Sulfate-collagen Protein Based scaffolds and Its Effects on the Neural Stem Cells

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Journal of Wuhan University of Technology(Materials Science) 2009年第S1期24卷 103-107页

作者：周昌伟王岩松 Department of Orthopedic Surgery Second Affiliated Hospital of Harbin Medical University

The objective of this study was to construct the heparin sulfate-collagen pro-tein-based scaffolds and to explore its effects on the proliferation and differentiation abilities of neural stem cells(NSCs).The heparin sulfate-collagen protein-based scaffolds were produced by injection molding/freezing/cross-linking *** proliferation ability was detected by MTT and the dif-ferentiation ability was detected by immunofluorescence *** results indicate that the heparin sulfate-collagen protein-based scaffolds with porous and longitudinal parallel structures are successfully *** NSCs grow well in the pores of scaffold and still maintain the prolif-eration and differentiation *** is concluded that the scaffolds possess stable porous and longi-tudinal parallel structures and great biocompatibility to *** combination of scaffold and NSCs may suggest a possible treatment strategy for spinal cord injury

关键词： neural stem cell nerve regeneration tissue engineering scaffold

Structural Assessment of Microsphere-based tissue Engineered scaffold from Poly（lactic acid）/β-Tricalcium Phosphate

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Structural Assessment of Microsphere-based Tissue Engineered...