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Mobilization of hematopoietic progenitor cells in patients with liver cirrhosis

World Journal of Gastroenterology 2010年第2期16卷 217-224页

作者： Ursula M Gehling Marc Willems Kathleen Schlagner Ralf A Benndorf Maura Dandri Jrg Petersen Martina Sterneck Joerg-Matthias Pollok Dieter K Hossfeld Xavier Rogiers Department of Hepatobiliary Surgery and Transplant Surgery University Hospital Hamburg-Eppendorf20246 HamburgGermany Department of Medicine University Hospital Hamburg-Eppendorf20246 HamburgGermany Institute of Experimental and Clinical Pharmacology and Toxicology University Hospital Hamburg-Eppendorf20246 HamburgGermany Gent University Hospital and Medical School 9000 GentBelgium

AIM:To test the hypothesis that liver cirrhosis is associated with mobilization of hematopoietic progenitor cells. METHODS:Peripheral blood samples from 72 patients with liver cirrhosis of varying etiology were analyzed by flow *** progenitor cell subsets were immunoselected and used for functional assays in vitro. Plasma levels of stromal cell-derived factor-1(SDF-1) were measured using an enzyme linked immunosorbent ***:Progenitor cells with a CD133 + /CD45 + CD14 + phenotype were observed in 61%of th *** 1%and 26%of the peripheral bloo mononuclear cells(MNCs)displayed this phenotype Furthermore,a distinct population of c-kit + progenito cells(between 1%and 38%of the MNCs)could b detected in 91%of the ***,18% of the patients showed a population of progenito cells(between 1%and 68%of the MNCs)that wa characterized by expression of breast cancer resistanc *** phenotypic analysis disclosed tha the circulating precursors expressed CXC chemokin receptor 4,the receptor for *** line with thi finding,elevated plasma levels of SDF-1 were presen in all patients and were found to correlate with th number of mobilized CD133 + progenitor cells.

关键词： CD133 antigen CD14 antigen c-kit protein Breast cancer resistance protein-1 protein Progenitor cells CXC chemokine receptor 4 stromal cell-derived factor-1 Liver cirrhosis

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C-X-C chemokine receptor type 7 antibody enhances neural plasticity after ischemic stroke

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Neural Regeneration Research 2023年第9期18卷 1976-1982页

作者： Xiao-Qian Zhang Xiao-Yin Wang Bing-Chao Dong Mei-Xuan Li Yu Wang Ting Xiao Shan-Shan Zhao Department of Neurology The First Hospital of China Medical UniversityShenyangLiaoning ProvinceChina Key Laboratory of Immunodermatology Ministry of HealthMinistry of EducationShenyangLiaoning ProvinceChina

stromal cell-derived factor-1 and its receptor C-X-C chemokine receptor 4(CXCR4) have been shown to regulate neural regeneration after *** r,whether stromal cell-derived factor-1 receptor CXCR7,which is widely distributed in the develo ping and adult central nervous system,participates in neural regeneration remains poorly unde *** this study,we established rat models of focal cerebral ischemia by injecting endothelin-1 into the cerebral co rtex and *** on day 7 after injury,CXCR7-neutralizing antibody was injected into the lateral ventricle using a micro drug delivery system for 6 consecutive *** results showed that CXCR7-neutralizing antibody increased the total length and number of sprouting co rticospinal tra ct fibers in rats with cerebral ischemia,increased the expression of vesicular glutamate transporter 1 and growth-related protein 43,marke rs of the denervated spinal cord synapses,and promoted the differentiation and maturation of oligodendrocyte progenitor cells in the *** addition,CXCR7 antibody increased the expression of CXCR4 in the striatum,increased the protein expression of RAS and ERK1/2 associated with the RAS/ERK signaling pathway,and im proved rat motor *** findings suggest that CXCR7 improved neural functional recovery after ischemic stroke by promoting axonal regeneration,synaptogenesis,and myelin regeneration,which may be achieved by activation of CXCR4 and the RAS/ERK1/2 signaling pathway.

关键词： axonal regeneration cerebral ischemia C-X-C chemokine receptor 4 CXCR7 antibody neural plasticity RAS/ERK pathway remyelination stroke stromal cell-derived factor-1 synaptogenesis

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Dedifferentiated human umbilical cord mesenchymal stem cell reprogramming of endogenous hSDF-1a expression participates in neural restoration in hypoxic-ischemic brain damagerats

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Genes & Diseases 2021年第3期8卷 331-343页

作者： Zhou Xiaoqin Liu Jia Dai Mengjie Gu Jialu Bi Yang Wang Yuting Hu Huajian Liu Bo Zhang Xiaojun Li Zhongyue Chen Jie Li Tingyu Zhan Xue Department of Gastroenterology Ministry of Education Key Laboratory of Child Development and DisordersNational Clinical Research Center for Child Health and Disorders(Chongqing)401122PR China International Science and Technology Cooperation Base of Child Development and Critical Disorders Children’sHospital of Chongqing Medical UniversityChongqing401122PR China Department of Pediatric Research Institute Chongqing Key Laboratory of Child Health and NutritionChildren’s Hospital of Chongqing Medical UniversityChongqing401122PR China Department of Neonatology Chongqing Health Center for Womenand Children400021PR China Child Health Centre of Northwest Women and Children’s Hospital USA

The transplantation of human umbilical cord mesenchymal stem cells(hUC-MSCs)can promote hypoxic-ischemic brain damage(HIBD)nerve repair,but finding suitable seed cells to optimize transplantation and improve treatment efficiency is an urgent problem to be *** this study,we induced hUC-MSCs into dedifferentiated hUC-MSCs(De-hUC-MSCs),and the morphology,stem cell surface markers,proliferation and tri-directional differentiation ability of the De-hUC-MSCs and hUC-MSCs were detected.A whole-gene chip was utilized for genome cluster,gene ontology and KEGG pathway analyses of differentially expressed ***-hUC-MSCs were transplanted into HIBD rats,and behavioral experiments and immunofluorescence assays were used to assess the therapeutic effect.A lentivirus vector for human stromal cell-derived factor-1(hSDF-1a)was constructed,and the role of hSDF-1a in the neuroprotective effect and mechanism of De-hUC-MSCs was ***-hUC-MSCs displayed similar cell morphology,stem cell surface marker expression,cell proliferation and even three-dimensional differentiation ability as hUC-MSCs but exhibited greater treatment potential in *** reprogramming mechanism of hSDF-1a participated in the dedifferentiation *** successfully constructing a stable hSDF-1a cell line,we found that De-hUC-MSCs might participate in nerve repair through the hSDF-1a/CXCR4/PI3K/Akt ***-hUC-MSCs reprogramming of endogenous hSDF-1a expression may mediate the hSDF-1a/CXCR4/PI3K/Akt pathway involved in nerve repair in HIBD rats.

关键词： Dediffer entiation Human umbilicalcord mesenchymal stem cells Hypoxic-ischemic brain damage Neurorestoration Reprogramming stromal cell-derived factor-1

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Effect of integrin α5β1 inhibition on SDF-l/CXCR4-mediated choroidal neovascularization

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International Journal of Ophthalmology(English edition) 2018年第5期11卷 726-735页

作者： Yang Lyu Wen-Qin Xu Li-Juan Sun Xiao-Yan Pan Jian Zhang Yu-Sheng Wang Department of Ophthalmology Eye Institute of China PLAXijing Hospitalthe Fourth Military Medical University Department of Ophthalmology General Hospital of Lanzhou Military Command Department of Biochemistry and Molecular Biology the Fourth Military Medical University

AIM：To investigate the roles of integrins in choroidal neovascularization（CNV） and their associations with the stromal cell-derived factor-1（SDF-1）/CXCR4 ***：CNV lesions were induced in mice using laser *** CNV induction,all animals were randomly assigned to：control,SDF-1,SDF-1＋age-related macular degeneration（AMD） 3100（CXCR4 inhibitor）,and SDF-1＋ATN161（integrin α5β1 inhibitor） groups;their effects on CNV progression were observed using hematoxylin eosin（HE） staining,fundus fluorescein angiography（FFA） grading and optical coherence tomography（OCT）,and their effects on CXCR4/integrin α5 expression were evaluated using Western blot and double immunofluorescence ***-exposed endothelial cells（ECs） were used to simulate CNV in vitro,they were treated with SDF-1,combined with CXCR4 siRNA/AMD3100 or ATN161,and expression of integrin α5,cell migration and tube formation were ***：Integrin subunit α5 increased at 3^ rd and 7^ th day and decreased at 14 ^th day in CNV mice,with no significant change of β***4 expression in CNV mice had persistent increase within 14 d after ***-1 treatment significantly promoted the CNV progression during 3-14 *** mean CNV length in AMD3100 andATN161 group at day 7 was 270.13 and 264.23 μm in HE images,significantly lower than the mean length in SDF-1（345.70 μm） ***3100 and ATN161 also significantly reduced thickness and leakage of CNV induced by *** integrin α5 positive area in SDF-1 group reached 2.31×104 μm^2,significantly higher than control（1.25×104 μm^2）,which decreased to 1.78×104 μm^2 after AMD3100 *** 61.36% of ECs in CNV lesions expressed α5 in SDF-1 group,which significantly decreased to 43.12% after AMD3100 *** vitro,integrin α5 peaked by 6 folds after 6 h of hypoxia exposure and CXCR4 gradually increased by up to 2.3 folds after 24 h of *** 25.12% of ECs expressed integrin α5 after SDF-1 stimulation,which

关键词： choroidal neovascularization endothelial cells stromal cell-derived factor-1 CXCR4 integrin α5β1 hypoxia

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Functions and mechanisms of chemokine receptor 7 in tumors of the digestive system

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World Journal of Clinical Cases 2020年第12期8卷 2448-2463页

作者： Qi Xin Quan Sun Chuan-Shan Zhang Qin Zhang Chun-Jun Li Department of Pathology Tianjin Third Central HospitalThe Third Central Clinical College of Tianjin Medical UniversityTianjin Key Laboratory of Extracorporeal Life Support for Critical DiseasesTianjin 300170China Tianjin Key Laboratory of Brain Science and Neural Engineering Academy of Medical Engineering and Translational MedicineTianjin UniversityTianjin 300072China Department of Hepatobiliary Surgery Tianjin Third Central HospitalThe Third Central Clinical College of Tianjin Medical UniversityTianjin Key Laboratory of Extracorporeal Life Support for Critical DiseasesTianjin 300170China Department of Endocrinology Health Management CenterTianjin Union Medical CenterNankai University Affiliated HospitalTianjin 300121China

Chemokine(C-X-C motif)receptor 7(CXCR7),recently termed ACKR3,belongs to the G protein-coupled cell surface receptor family,binds to stromal cellderived factor-1[SDF-1,or chemokine(C-X-C motif)ligand 12]or chemokine(CX-C motif)ligand 11,and is the most common chemokine receptor expressed in a variety of cancer ***-1 binds to its receptor chemokine(C-X-C motif)receptor 4(CXCR4)and regulates cell proliferation,survival,angiogenesis and *** recent years,another new receptor for SDF-1,CXCR7,has been discovered,and CXCR7 has also been found to be expressed in a variety of tumor cells and tumor-related vascular endothelial *** studies have shown that CXCR7 can promote the growth and metastasis of a variety of malignant tumor *** CXCR4,CXCR7 exhibits a slight modification in the DRYLAIV motif and does not induce intracellular Ca^2+release following ligand binding,which is essential for recruiting and activating G ***7 is generally thought to work in three ways:(1)Recruitingβ-arrestin 2;(2)Heterodimerizing with CXCR4;and(3)Acting as a“scavenger”of SDF-1,thus lowering the level of SDF-1 to weaken the activity of *** the present review,the expression and role of CXCR7,as well as its prognosis in cancers of the digestive system,were investigated.

关键词： stromal cell-derived factor-1 Chemokine(C-X-C motif)receptor 7 Chemokine(C-X-C motif)receptor 4 Carcinoma Digestive system

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CXCR4/SDF-1 axis is involved in lymph node metastasis of gastric carcinoma

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World Journal of Gastroenterology 2011年第19期17卷 2389-2396页

作者： Bao-Cheng Zhao Zhen-Jun Wang Wei-Zheng Mao Hua-Chong Ma Jia-Gang Han Bo Zhao Hui-Min Xu Department of General Surgery Beijing Chaoy- ang Hospital Capital Medical University Beijing 100020 China Department of General Surgery Qingdao Mu- nicipal Hospital Qingdao University Medical College Qingdao 266071 Shandong Province China Department of General Surgery Weifang People's Hospital Weifang 261041 Shangdong Province China

AIM:To investigate the role of CXC chemokine receptor-4 (CXCR4) and stromal cell-derived factor-1 (SDF-1) in lymph node metastasis of gastric ***:In 40 cases of gastric cancer,expression of CXCR4 mRNA in cancer and normal mucous membrane and SDF-1 mRNA in lymph nodes around the stomach was detected using quantitative polymerase chain reaction (PCR) (TaqMan) and immunohistochemistric ***-7901 and MGC80-3 cancer cells were used to investigate the effect of SDF-1 on cell proliferation and ***:Quantitative reverse transcription PCR and immunohistochemistry revealed that the expression level of CXCR4 in gastric cancer was significantly higher than that in normal mucous membrane (1.6244 ± 1.3801 vs 1.0715 ± 0.5243,P cell lines expressed CXCR4 and showed vigorous proliferation and migratory responses to ***3100 (a specific CXCR4 antagonist) was also found to effectively reduce the migration of gastric cancer ***:The CXCR4/SDF-1 axis is involved in the lymph node metastasis of gastric ***4 is considered as a potential therapeutic target in the treatment of gastric cancer.

关键词： Gastric carcinoma Chemokines stromal cell-derived factor-1 CXC chemokine receptor-4 Lymph node metastasis

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Inhibitory effect of triptolide on lymph node metastasis in patients with non-Hodgkin lymphoma by regulating SDF-1/CXCR4 axis in vitro

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Acta Pharmacologica Sinica 2006年第11期27卷 1438-1446页

作者： Chun ZHANG Guo-hui CUI Fang LIU Qiu-ling WU Yan CHEN~2 Department of Hematology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China DepartmentofHematology UnionHospitalTongjiMedicalCollegeHuazhongUniversityofScienceandTechnologyWuhan430022China

Aim:To investigate the antiproliferative effect of triptolide on B-NHL cell line Rajicells,to study its effect on lymph node metastasis in patients with non-Hodgkin’slymphoma(NHL)in vitro,and to explore the underlying mechanism regulatingSDF-1/CXCR4 ***:The effects of triptolide on the growth of Raji cellswere studied by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium(MTT)*** effects of triptolide on SDF-1 mRNA expression in lymph node stromalcells from patients with NHL were determined by reverse transcriptase-polymerasechain reaction(RT-PCR).The effects of triptolide on CXCR4 expression on lym-phoma cells freshly isolated from the lymph nodes of these patients were studiedby flow cytometric *** assays were performed to observe theeffects of triptolide on migration of primary lymphoma cells towards recombinanthuman SDF-1α(rhSDF-1α)or cultured lymph node stromal cells in ***:Triptolide inhibited the proliferation of B-NHL cell line Raji cells in a dose-andtime-dependent manner with a 24-h IC50value of 43.06 nmol/L and a 36-h IC50valueof 25.08 nmol/*** expression of SDF-1α mRNA in lymph node stromal cellsobtained from patients with NHL was decreased after treatment by triptolide atconcentrations of 25 and 50 nrnol/L for 24 *** cytometry analysis showed thatthe CXCR4 expression on primary lymphoma cells were downregulated graduallyin a dose-dependent manner following triptolide *** assaysrevealed that the migration of freshly isolated lymphoma cells towards either rhSDF-1or cultured lymph node stromal cells was markedly inhibited by the addition oftriptolide in vitro,and the inhibition was ***:Triptolidecan inhibit the proliferation of B-NHL cell line Raji ***,triptolide isable to inhibit the migration of lymphoma cells via lymph nodes in *** antitumor mechanisms of triptolide are related to the antiproliferativeeffect and the blockage of SDF-1/CXCR4

关键词： triptolide Raji metastasis non-Hodgkin’s lymphoma stromal cell-derived factor-1 CXCR4

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Effect of Fufang Xueshuantong Capsule(复方血栓通胶囊) on A Rat Model of Retinal Vein Occlusion

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Chinese Journal of Integrative Medicine 2011年第4期17卷 296-301页

作者：袁源智袁非徐庆玥俞笳黎蕾张菊莉 Department of Ophthalmology Zhongshan HospitalFudan University Department of Ophthalmology EyeEarNose and Throat HospitalFudan University Nursing Department Zhongshan HospitalFudan University

Objective：To establish a retinal vein occlusion（RVO） animal model and observe the therapeutic effect of a Chinese herbal composition（Fufang Xueshuantong Capsule,复方血栓通胶囊,FXC） in ischemic retinal ***：Fifteen adult male Sprague-Dawley rats underwent laser photothrombosis to induce RVO on their right eyes and were subsequently randomized to receive FXC（the intervention group,n=7） or placebo treatment（the control group,n=8）.Fundus fluorescein angiography was performed after 2,4 and 8 weeks of ***-time reverse transcription-PCR was used to quantify the mRNA expression of vascular endothelial growth factor（VEGF） and stromal cell-derived factor-1（SDF-1）.The main outcomes were the mRNA copies of VEGF and SDF-1 and the counts of RVO ***：Laser photothrombosis procedure induced typical lesions of RVO,including hemorrhage,leakage,retinal detachment,capillary non-perfusion,filling defect of retinal vessels,and lateral circulation/dilation of small *** retinal lesions were associated with an increased expression of VEGF（P0.05）.No significant change of SDF-1 expression was *** with the control group,the intervention group had numerically fewer RVO lesions at week 2（1.71±0.76 vs.3.50±1.51,t=-2.82,P0.05）.The benefit of intervention remained at weeks 4 and ***：A rat model of laser photothrombosis-induced RVO was established and an increase in the VEGF expression was observed in the retinal *** FXC had therapeutic benefit in improving retinal lesions in the rat model of RVO.

关键词： Fufang Xueshuantong Capsule retinal vein occlusion animal model stromal cell-derived factor-1 vascular endothelial growth factor

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The expressions of SDF-1αmRNA and protein are up-regulated by electroacupuncture and contribute to revascularization in rats brain of focal cerebral ischemia/reperfusion

The expressions of SDF-1αmRNA and protein are up-regulated ...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Wang ZHAO Yong LUO~* Department of Neurology The First Affiliated Hospital Chongqing Medical University Chongqing Key Laboratory of Neurology Chongqing 400016 China

＜正＞Objective Both angiogenesis and vasculogenesis are very important in revascularization of cerebral ischemic *** studies have demonstrated that BM-derived endothelial progenitor cells（EPCs） functionally contribute to vasculogenesis during cerebral *** cell-derived factor-1α（SDF-1α） and its receptor play a crucial role in mobilization,migration,homing of ***,few studies have shown about electroacupuncture（EA） promoting revascularization in the field of vasculogenesis of cerebral *** study focused on the mechanism of EA promoting revascularization in the rat brain of focal cerebral ischemia/*** The SD rat model of focal cerebral ischemia /reperfusion was made by filament *** were randomly divided into control group,model group,and EA *** group and EA group were divided into 5 subgroups according to accept reperfusion 1 d,3 d,7 d,14 d,21 d after 1h *** was given at bilateral "Hegu" point（LI 4） in the EA *** expression of SDF-1αmRNA was detected with RT-PCR in the 3 d,7 d,14 d *** immunohistochemical method was employed to detect the expression of stromal cell-derived factor -la（SDF-1α） protein,microvessel *** Compared with the control group,expression of SDF-1αprotein increased in the model group and EA group（P ＜ 0.05）;Compared with the model group,the expression of SDF-1αmRNA increased in the 3d,7d,14d subgroups（P ＜ 0.05）.The SDF-1αprotein expression and microvessel count increased but without statistical significance in the 1d subgroup（P ＞ 0.05）,however,it had statistical significance in the 3 d,7 d,14 d,21 d subgroups（P ＜ 0.05）,the date show a peak of SDF-1αexpression in 7d. Conclusion EA may promote vasculogenesis by increaseing the expression of SDF-1αmRNA and protein in the rats brain of focal cerebral ischemia /reperfusion.

关键词： electroacupuncture focal cerebral ischemia/reperfusion stromal cell-derived factor-1 vascular regeneration "Hegu" point

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