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Differential regulation of glutathione S-transferase Yb1mRNA levels in rat prostate, liver and brain by androgen

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Cell Research 1995年第2期5卷 235-242页

作者： ZHANG YUAN YOUDUAN ZHANG CHAWNSHANG CHANG YONGLIAN ZHANG(Shanghai Institute of Biochemistry, Academia Sinica, 320Yueyang Road, Shanghai 200031, China.)(Department of Human Oncology, University of Wisconsin, 600 Highland Avenue, Madison, WI 53792, USA.) Shanghai Institute of Biochemistry Academia Sinica Shanghai China Department of Human Oncology University of Wiscon-sin USA

Northern blot analysis of glutathione S-transferase (GST) Yb1 mRNA in different tissues of male and female rats revealed that its tissue-specific transcription patterns were highly sex hormone related. Although the GST Yb1 mRNA could be detected in most of the tissues examined at various levels, the highest abundance was observed in the ventral prostate, uterus and liver, which were the main target tissue for androgen, estrogen and glucocorticoid respectively The effect of androgen on the transcription of GST Yb1 was also tissue-specific. Since androgen withdrawal by castration caused the up-regulation of GST Yb1 mRNA in the ventral prostate but down-regulation in the liver and no effect in the brain, evaluation of this system for studying the regulation mechanisms of gene expression by which androgen exerts its differential effects has been discussed.

关键词： GST Yb1 mRNA androgen regulation rat prostate rat liver rat brain

MicroRNA expression profile and lipid metabolism characteristics in liver of rat undergoing high-fat diet

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BIOCELL 2019年第3期43卷 129-137页

作者： WEN JING CHUANFEN LI YINGLI LU LIANSHI FENG College of Physical Education Shandong Normal UniversityJinan250014China College of Life Science Shandong Normal UniversityJinan250014China Department of Neurology The 960th Hospital of the Joint Logistic Support Force of the Chinese People’s Liberation ArmyJinan250031China Biology Center China Institute of Sport ScienceBeijing100061China

This study aimed to investigate the microRNA expression profile and the characteristics of lipid metabolism in the livers of rats undergoing a high-fat *** male Sprague-Dawley(SD)rats were divided into a standard chow group(C group,N=10)and a high-fat diet group(H group,N=40).After 12 weeks,the rat body weight,body length,fat mass,and serum lipid concentration were *** expression profile of microRNAs and the gene and protein expression levels involved in lipid metabolism in rat liver were *** fat and serum lipid concentrations were all significantly higher in the H group than those in the C group(pliver(prat liver(prate metabolism and increase ectopic fat accumulation through let-7 family *** high-fat diet for 12 weeks decreased lipid metabolism level in the liver,thereby decreasing fatty acid synthesis,oxidation,and transport by down-regulating the PPARα,FAS,CPT1A,FAT/CD36,and ApoAI protein levels.

关键词： High-fat diet Lipid metabolism MicroRNA expression rat liver Serum lipid concentration

Protective Effects of Salubrinal on liver Injury in rat Models of Brain Death

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Chinese Medical Journal 2015年第11期128卷 1523-1528页

作者： Tao Wang Shui-Jun Zhang Sheng-Li Cao Wen-Zhi Guo Bing Yan Hong-Bo Fang Department of Hepatobiliary and Pancreatic Surgery the First Affiliated Hospital of Zhengzhou University Zhengzhou Henan 450001 China Henan Key Laboratory of Digestive Organ Transplantation Zhengzhou Henan 450001 China

Background： Previous studies have indicated that endoplasmic reticulum stress participates in and mediates liver injury and apoptosis in brain-dead （BD） rats. In this study, we observed the effect ofsalubrinal （Sal, Sigma, USA） on liver cells in BD rats and explored its relevant mechanisms. Methods： Thirty Sprague-Dawley rats were equally randomized into three groups： BD group, Sal group, and DMSO group. The BD models were established by increasing intracranial pressure in a modified, slow, and intermittent way. In the drug groups, Sal was administered I h before the induction of BD. After modeling was completed, the blood and liver samples were harvested. CHOP and Caspase-12 mRNA expression was detected using quantitative polymerase chain reaction. PKR-like ER kinase （PERK）, P-eukaryotic translation initiation factor 2a （elF2a）, elF2a, CHOP and caspase-12 expression was detected using western blotting （WB）. CHOP and caspase-12 distribution and expression in liver tissues were determined using immunohistochemistry （IHC）. Alanine aminotransferase and aspartate aminotransferase level were detected using an automatic biochemical analyzer. Hepatic cell apoptosis was detected using TUNEL. The results were analyzed using Quantity-one v4.62 software （Bio-Rad, USA）. Results： CHOP and caspase-12 expression and PERK, elF2a, and P-elF2a protein expression showed no significant difference between BD group and DMSO group. Compared with BD group, Sal group had a significantly higher P-elF2C level and a lower P-PERK level 2 h and 6 h alter BD （P 〈 0.05）. However, eIF2a expression showed no significant difference （P 〉 0.05）. After the Sal treatment, CHOP and caspase- 12 mRNA expression significantly decreased 4 hatter BD （P 〈 0.05）. WB and IHC indicated that CHOP and caspase- 12 expression also significantly decreased after Sal treatment. Sal was associated with improved liver function and decreased hepatic cell apoptosis. Conclusions： Sal can significantly reduce apoptosis in hepa

关键词： Apoptosis Brain Death Endoplasmic Reticulum Stress rat liver Salubrinal

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Damage to Wistar rat livers after hypo-fractionated radiation and oxaliplatin

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Oncology and Translational Medicine 2015年第3期1卷 130-134页

作者： Mingxuan Chen Yongheng An Hongsheng Yu Yifan Li Xiaoman Chen Department of Oncology The Affiliated Hospital of Qingdao University Department of Breast center The Affiliated Hospital of Qingdao University

Objective The purpose of this study was to clarify whether hypo-fractionated radiation therapy combined with oxaliplatin can aggravate liver damage, in order to determine its safety for clinical application. Methods Eighty Wistar rats were randomly divided into four groups： the control group, the chemotherapy treatment group, the radiation treatment group, and the concurrent chemoradiotherapy group. The rats＇ liver tissues were then collected for histological evaluation at the first, second, fourth, sixth, and eight week after irradiation. The tissues were histologically evaluated using hematoxylin and eosin staining, and immunohis-tochemistry to analyze the expression of Bcl-2 and Bax. Results Histological examination revealed swollen hepatocellular cells in the experimental groups, with visible liver degeneration and necrosis. Alanine aminotransferase and aspartate aminotransferase levels were significantly different between the groups （F = 85.869 and 214.663; P 〈 0.001）. The intra-group expressions of Bcl-2 and Bax were also significantly different between each time point （F = 6.047 and 43.344; P 〈 0.05）. Bax expression was significantly different between each group （F = 8.122; P 〈 0.05）, although no inter-group differences were observed for Bol-2 expression （F = 0.808; P 〉 0.05）. Conclusion Chemoradiotherapy may aggravate liver injury, possible via overexpression of Bcl-2 and reduced expression of Bax. Therefore, this treatment should be used carefully in the clinic.

关键词： radiation effects concurrent chemoradiotherapy rat liver Bcl-2 Bax

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Transcriptome profiling analysis reveals multiple modulatory effects of Ginkgo biloba extract in the liver of rats on a high-fat diet

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Transcriptome profiling analysis reveals multiple modulatory...

ALTEratIONS　IN　THE　PROTEIN　SYNTHESIS　AND　CONTENTS　OF　RIBOSOME　AND　POLYSOME　IN　liverOF　SELENIUM－DEFICIENT　ratS

中药药效提高与中药饮片质量控制交流研讨会

作者： Xiaomei Gu~(1,*),Zuoquan Xie~(2,*),Qi Wang~(1,*),Gang Liu~1,Yi Qu~1,Lu Zhang~(1,2),Jiahu Pan~3,Guoping Zhao~(1,4) and Qinghua Zhang~(1,2,4) 1 National Engineering Center for Biochip at Shanghai,China 2 State Key Laboratory of Medical Genomics and Shanghai Institute of Hematology,Ruijin Hospital,Shanghai Jiaotong University School of Medicine,China 3 School of Pharmacy,Fudan University,Shanghai,China 4 MOST-Shanghai Key Laboratory of Disease and Health Related Genomics,China

Leaf extract of Ginkgo biloba(GBE) is increasingly used as a herbal medicine for the treatment of neurodegenerative,cardiovascular and cerebrovascular *** studies have demonstrated many protective effects of GBE in neurons,the endothelium and *** this study,we investigated the molecular mechanisms underlying the effects of GBE in disorders induced by long-term exposure to a high-fat diet(HFD).rats were fed an HFD with or without the GBE product GBE50 for 19 *** found that GBE50 reduced the development of fatty liver induced by an HFD and inhibited the commonly observed elevation of serum cholesterol and lactate dehydrogenase *** profiling analysis showed that several genes were modulated by GBE50 in liver,including those involved in lipid metabolism,carbohydrate metabolism,vascular constriction,ion transportation,neuronal systems and drug ***,a number of genes coding for proteins involved in cholesterol metabolism were repressed,and some were *** acid biosynthesis appeared to be repressed,whereas fatty acid metabolism appeared to be *** conclusion,using transcriptome profiling analysis,we demonstrated the molecular basis for the pleiotropic effects of GBE50,particularly those involved in lipid *** study provided new clues for further pharmacological study of GBEs.

关键词： Ginkgo biloba extract high-fat diet rat liver regulation transcriptome

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Journal of Pharmaceutical Analysis 1996年第1期10卷 17-21页

作者：毕红李同良贾锡安赵君庸

In the present experiments the changes in levels of ribosome,polysome and 3H-leucine incorporation rate in liver post-mitochondrial supernatant (PM-supernatant) were investigated in Sedericient and Se-supplented *** results demonstrated that the amounts of ribosome and polysome as well as the ratio of polysome to ribosome in liver PM-supernatant from the Se-deficient rats were all remarkahly *** the meantime,the rate of protein synthesis expressed as radioactivity or 3H-leucine incorporated into protein in the PM-supernatant system also decreased *** results suggest that the decreases of ribosomes and proportion of ribosomal aggregates in PM-supernatant may be responsible for the decrease of the protein synthesis activity in liver of the Se-deficient animals.

关键词： selenium ribosomes polysomes protein synthesis rat liver

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聚焦超声对鼠肝细胞超微结构的影响

陕西师大学报(自然科学版) 1989年第2期 93-94页

作者：袁仕取闵俐刘全宏任耀辉陕西师范大学生物系陕西师范大学生物系

超声诊断、超声理疗和超声外科等方面已有广泛的研究.但是,超声对肝细胞的作用除国外有少数报导以外,国内关于这一方面的研究甚少.为了研究超声作用下肝细胞坏死前的超微结构变化,作者进行了聚焦超声辐照在体大白鼠肝的实验. 1 材料和... 详细信息

超声诊断、超声理疗和超声外科等方面已有广泛的研究.但是,超声对肝细胞的作用除国外有少数报导以外,国内关于这一方面的研究甚少.为了研究超声作用下肝细胞坏死前的超微结构变化,作者进行了聚焦超声辐照在体大白鼠肝的实验. 1 材料和方法实验用健康雄性大白鼠24只,为同一品系,体重200～250克,随机分为四组.组一、三为实验组,组二、四为对照组.四组动物均以乙醚麻醉,常规外科手术切开腹壁,暴露肝脏.实验组以强度5W/cm~2,频率1.06MHz的聚焦超声辐照肝的中叶尖端3分钟.对照组作同样处理,唯独不启动超声源,也称为“假”照射.组一、二和三、四“真”“假”照射分别在宰杀

关键词： focused ultrasound rat liver nucleous