MicroRNA expression profile and lipid metabolism characteristics in liver of rat undergoing high-fat diet

作     者：WEN JING CHUANFEN LI YINGLI LU LIANSHI FENG 

作者机构：College of Physical EducationShandong Normal UniversityJinan250014China College of Life ScienceShandong Normal UniversityJinan250014China Department of NeurologyThe 960th Hospital of the Joint Logistic Support Force of the Chinese People’s Liberation ArmyJinan250031China Biology CenterChina Institute of Sport ScienceBeijing100061China 

出 版 物：《BIOCELL》 (生物细胞（英文）)

年 卷 期：2019年第43卷第3期

页      面：129-137页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by Natural Science Foundation Youth Project of Shandong Province,China(Grant No.ZR2014CQ026 to Dr.Wen Jing) The Science Foundation for the Youth of China Institute of Sport Science,China(Grant No.13-19 to Dr.Ying-li Lu) 

主　　题：High-fat diet Lipid metabolism MicroRNA expression Rat liver Serum lipid concentration 

摘      要：This study aimed to investigate the microRNA expression profile and the characteristics of lipid metabolism in the livers of rats undergoing a high-fat *** male Sprague-Dawley(SD)rats were divided into a standard chow group(C group,N=10)and a high-fat diet group(H group,N=40).After 12 weeks,the rat body weight,body length,fat mass,and serum lipid concentration were *** expression profile of microRNAs and the gene and protein expression levels involved in lipid metabolism in rat liver were *** fat and serum lipid concentrations were all significantly higher in the H group than those in the C group(p0.05 or p0.01).The expression of 10 microRNAs showed significant differences in the liver(p0.05).In particular,the let-7 family expression levels significantly increased(p0.05)in the H group compared with those in the C *** with the C group,the high-fat diet resulted in low FAS,CPT1A,and ApoAI mRNA expression levels(p0.05 or p0.01)and high PPARαand FAT/CD36 mRNA expression levels in the H group rat liver(p0.01).Meanwhile,the protein PPARα,FAS,CPT1A,FAT/CD36,and ApoAI expression levels were all significantly lower in the H group than those in the C group(p0.05 or p0.01).In conclusion,the high-fat diet increased the body fat and serum lipid levels and altered the 10 microRNA expression levels in the *** high-fat diet may affect hepatic carbohydrate metabolism and increase ectopic fat accumulation through let-7 family *** high-fat diet for 12 weeks decreased lipid metabolism level in the liver,thereby decreasing fatty acid synthesis,oxidation,and transport by down-regulating the PPARα,FAS,CPT1A,FAT/CD36,and ApoAI protein levels.