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Effects of progesterone on T-type-Ca^(2+)-channel expression in purkinje cells

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Neural Regeneration Research 2022年第11期17卷 2465-2471页

作者： Annika Eickhoff Jonas Tjaden Sarah Stahlke Matthias Vorgerd Verena Theis Veronika Matschke Carsten Theiss Department of Cytology Institute of AnatomyRuhr-University BochumBochumGermany Department of Neurology Neuromuscular Center RuhrgebietUniversity Hospital BergmannsheilRuhr-Universität BochumBochumGermany

Plasticity of cerebellar purkinje cells(PC)is influenced by progeste rone via the classical progeste rone receptors PR-A and PR-B by stimulating dendritogenesis,spinogenesis,and synaptogenesis in these *** PC cultures were used to analyze progeste rone effects at a molecular level on the voltage-gated T-type-Ca^(2+)-channels Ca_(v)3.1,Ca_(v)3.2,and Ca_(v)3.3 as they helped determine neuronal plasticity by regulating Ca^(2+)-influx in neuronal *** results showed direct effects of progesterone on the mRNA expression of T-type-Ca^(2+)-channels,as well as on the protein kinases A and C being involved in downstream signaling pathways that play an important role in neuronal *** the mRNA expression studies of T-type-Ca^(2+)-channels and protein kinases of the signaling cascade,laser microdissection and purified PC cultures of diffe rent maturation stages were *** staining was also performed to characte rize the localization of T-type-Ca^(2+)-channels in *** rimental progesterone treatment was performed on the purified PC culture for 24 and 48 *** results show that progesterone increases the expression of Ca_(v)3.1 and Ca_(v)3.3 and associated protein kinases A and Cin PC at the mRNA level within 48 hours after treatment at *** effects extend the current knowledge of the function of progesterone in the central nervous system and provide an explanatory approach for its influence on neuronal plasticity.

关键词： Ca_(v)3.1 Ca_(v)3.2 Ca_(v)3.3 neuronal plasticity progesterone protein kinase A protein kinase C purkinje cells rat cerebellum T-type-Ca^(2+)-channels

Role of 5-hydroxytryptamine expression in cerebellar purkinje cells in obstructive sleep apnea syndrome

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Neural Regeneration Research 2012年第8期7卷 606-610页

作者： Liping Peng Jinghua Wang Lihong Zhang Pan Liu Min Wang Min Huang Shengnan Liu Pingping He Li Cui Mingxian Li Shao Wang Department of Pneumology First Affiliated Hospital of Jilin University Changchun 130021 Jilin Province China Key Laboratory of Pathobiology Ministry of Education Jilin University Changchun 130021 Jilin Province China Department of Physiology Norman Bethune College of Medicine Jilin University Changchun 130021 Jilin Province China Department of Neurology First Affiliated Hospital of Jilin University Changchun 130021 Jilin Province China

In the present study, electrical stimulation to the rat insular cortex induced apnea or respiratory disturbance, reduced amplitude of genioglossal electromyogram, and decreased electromyogram integrals. In addition, arterial blood gas analysis showed arterial blood acidosis, reduced pH values, increased alkali reserve negative values, decreased peripheral blood 5-hydroxytryptamine content, and increased 5-hydroxytryptamine expression in cerebellar purkinje cells. Following lidocaine injection to block the habenular nucleus, abnormalities in breath, genioglossal electromyogram, and blood gas values disappeared, and peripheral blood 5-hydroxytryptamine content returned to levels prior to electric stimulation. However, 5-hydroxytryptamine expression in cerebellar purkinje cells remained high. The results suggested that 5-hydroxytryptamine expression in purkinje cells did not correlate with ventilation function involving insular cortex and habenular nucleus.

关键词： sleep apnea obstructive cerebellum 5-hydroxytryptamine insular cortex habenular nucleus purkinje cells

Coexistence of dopamine-beta-hydroxylase and activated protein-2 alpha in rat cerebellar purkinje cells

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Neural Regeneration Research 2009年第6期4卷 431-435页

作者： Kejian Wang Wei Li Shanquan Sun Zhongqin Ren Guiqiong He Department of Human Anatomy Coflege of Basic Medicine Chongqing Medical University Chongqing 400016 China Institute of Neuroscience Chongqing Medical University Chongqing 400016 China

BACKGROUND： Tyrosine hydroxylase and phenylethanolamine-n-methyl transferase expression coexist in purkinje cells of the rat cerebellum. Numerous reports have also been published addressing whether dopamine-beta-hydroxylase （DBH） expression exists in cerebellar purkinje cells. OBJECTIVE： To investigate the coexistence of DBH and activator protein-2α expression in rat cerebellar purkinje cells. DESIGN, TIME AND SETTING： A cell morphological study was performed at the Institute of Neuroscience, Chongqing Medical University, China in May 2007. MATERIALS： Ten healthy Wistar rats, of either gender, aged 14 weeks, served as experimental animals. Rabbit anti-mouse DBH, goat anti-mouse activator protein-2α and rabbit anti-mouse β-actin （Santa Cruz Biotechnology, Inc., USA）, horseradish peroxidase-labeled goat anti-rabbit IgG, FITC-labeled mouse anti-rabbit IgG, and Cy3-labeled mouse anti-goat IgG （Boster, Wuhan, China）, were used in this study. METHODS： Immunohistochemical staining was used to measure the expression of DBH or activator protein-2α, with double-label immunofluorescence being employed to determine coexpression of both, in the cerebellum of 5 randomly selected rats. Western blot assay was utilized to determine the expression of DBH and activator protein-2α in the cerebellum of the remaining 5 rats. MAIN OUTCOME MEASURES： Expression, localization and coexistence of DBH and activator protein-2α in the cerebellum were measured separately. RESULTS： Immunohistochemical staining demonstrated that cerebellar purkinje cells stained positive for DBH and activator protein-2α. Western blot assay also demonstrated DBH and activator protein-2α expression in the cerebellum. Double-labeling immunofluorescence showed the coexistence of DBH and activator protein-2α in cerebellar purkinje cells. CONCLUSION： Norepinephrine and activator protein-2α coexist in rat cerebellar purkinje cells.

关键词： purkinje cells cerebellum dopamine-beta-hydroxylase activator protein-2α

Effects of Maternal Marginal Iodine Deficiency on Interactions between Cerebellar Bergmann Glia cells and purkinje cells in Rat Offspring

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Biomedical and Environmental Sciences 2017年第12期30卷 932-937页

作者： YU Ye DONG Jing WANG Yuan WANG Yi MIN Hui SHAN Zhong Yan TENG Wei Ping CHEN Jie Department of Occupational and Environmental Health School of Public Health China Medical University Department of Endocrinology and Metabolism Liaoning Provincial Key Laboratory of Endocrine Diseases the First Hospital of China Medical University

Iodine deficiency （ID） during early pregnancy has an adverse effect on children＇s psychomotor and motor function but the mechanism has not been clarified. Therefore, our aim was to study the effect of maternal marginal ID on cerebellar neurodevelopment and the underlying mechanism. After obtaining marginal ID rats, we examined interactions between Bergmann gila cells （BGs） and purkinje cells （PCs） using immunofluorescence and expression of the glutamate transporter and receptor by western blot. Our results showed that marginal ID reduced the number of contacted points between BGs and PCs,

关键词： Effects of Maternal Marginal Iodine Deficiency Cerebellar Bergman purkinje cells

Curcumin alters motor coordination but not total number of purkinje cells in the cerebellum of adolescent male Wistar rats

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Journal of Integrative Medicine 2013年第1期11卷 32-38页

作者： Ginus Partadiredja Sutarman Taufik Nur Yahya Christiana Tri Nuryana Rina Susilowati Department of Physiology Faculty of Medicine Universitas Gadjah MadaYogyakarta 55281 Indonesia Department of Anatomy Embryology and Anthropology Faculty of Medicine Universitas Gadjah MadaYogyakarta 55281 Indonesia Department of Histology and Cell Biology Faculty of Medicine Universitas Gadjah MadaYogyakarta 55281 Indonesia

OBJECTIVE： The present study aimed at coordination and the estimate of the total investigating the effects of curcumin on the motor number of cerebellar purkinje cells of adolescent Wistar rats exposed to ethanol. METHODS： The total of 21 male Wistar rats aged 37 d old were divided into three groups, namely ethanol, ethanol-curcumin, and control groups. The ethanol group received 1.5 g/kg ethanol injected intraperitoneally and water given per oral; the ethanol-curcumin group received 1.5 g/kg ethanol injected intraperitoneally and curcumin extract given per oral; the control group received saline injection and oral water. The treatment was carried out daily for one month, after which the motor coordination performance of the rats was examined using revolving drum apparatus at test days 1, 8, and 15. The rats were finally sacrificed and the cerebellum of the rats was further processed for stereological analysis. The estimate of the total number of purkinje cells was calculated using physical fractionator method. RESULTS： The ethanol-curcumin group performed better than both ethanol and control groups in the motor coordination ability at day 8 of testing （P〈 0.01）. No purkinje cell loss was observed as a result of one month intraperitoneal injection of ethanol. CONCLUSION： Curcumin may exert beneficial effects on the motor coordination of adolescent rats exposed to ethanol via undetermined hormetic mechanisms.

关键词： curcumin purkinje cells antioxidants rats, Wistar

CD4 and CD8 T cells mediate distinct lethal meningoencephalitis in mice challenged with Tacaribe arenavirus

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Cellular & Molecular Immunology 2017年第1期14卷 90-106页

作者： Derek DC Ireland Cecilia Tami Joao Pedras-Vasconcelos Daniela Verthelyi Division of Biologics Review and Research-III Office of Biotechnology Center for Drug Evaluation and Research Food and Drug Administration Silver Spring MD 20993 USA

Neonates are at increased risk of viral encephalopathies that can result in neurological dysfunction, seizures, permanent disability and even death. The neurological damage results from the combined effect of the virus and the immune response it elicits, thus finding tools to facilitate viral clearance from central nervous system （CNS） while minimizing neuron damage remains a critical challenge. Neonatal mice inoculated intraperitoneally with Tacaribe virus （TCRV） develop seizures, hindlimb paralysis and death within 15 days of inoculation. TCRV localizes to the CNS within days of challenge, primarily infecting astrocytes in the cerebellum and brain stem. We show that infection leads to inflammation, T cell and monocyte infiltration into the cerebellar parenchyma, apoptosis of astrocytes, neuronal degeneration and loss of purkinje cells. Infiltrating antigen-specific T cells fail to clear the virus but drive the disease, as T-cell-deficient CD3ε KO mice survive TCRV infection with minimal inflammation or clinical manifestations despite no difference in CNS viral loads in comparison with T-cell sufficient mice. CD8＋ T cells drive the pathology, which even in the absence of CD4＋ T-cell help, infiltrate the parenchyma and mediate the apoptotic loss of cerebellar astrocytes, neurodegeneration and loss of purkinje cells resulting in loss of balance, paralysis and death. CD4＋ T cells are also pathogenic inducing gliosis and inflammation in the cerebellum and cerebrum that are associated with wasting and death several weeks after CD4＋ T-cell transfer. These data demonstrate distinct pathogenic effects of CD4＋ and CD8＋ T cells and identify them as possible therapeutic targets.

关键词： Astrocytes CD4 T cells CD8 T cells encephalitis purkinje cells virus

A bacterial artificial chromosome transgenic mouse model for visualization of neurite growth

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Science China(Life Sciences) 2015年第4期58卷 373-378页

作者： TAO Tao CHEN Chen SUN Jie PENG YaJing ZHU MinSheng Model Animal Research Center and MOE Key Laboratory of Model Animal for Disease Study Nanjing University

Class Ⅲ β-tubulin （Tubb3） is a component of the microtubules in neurons and contributes to microtubule dynamics that are required for axon outgrowth and guidance during neuronal development. We here report a novel bacterial artificial chromosome （BAC） transgenic mouse line that expresses Class Ⅲ β-tubulin fused to mCherry, an improved monomeric red fluorescent protein, for the visualization of microtubules during neuronal development. A BAC containing Tubb3 gene was modified by insertion of mCherry complementary DNA downstream of Tubb3 coding sequence via homologous recombination, mCherry fusion protein was expressed in the nervous system and testis of the transgenic animal, and the fluorescent signal was observed in the neurons that located in the olfactory bulb, cerebral cortex, hippocampal formation, cerebellum, as well as the retina. Besides, Tubb3-mCherry fusion protein mainly distributed in neurites and colocalized with endogenous Class Ⅲ β-tubulin The fusion protein labels purkinje cell dendrites during cerebellar circuit formation. Therefore, this transgenic line might be a novel tool for scientific community to study neuronal development both in vitro and in vivo.

关键词： Tubb3 mCherry BAC transgenic mouse neuronal development purkinje cells