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Aquaculture and Fisheries 2023年第4期8卷 451-456页

作者： John A.Theodorou Maria Makri Xanthi Douvi Alexis Ramfos Efthimios Spinos Department of Animal Production Fisheries&AquacultureUniversity of PatrasMesolongi30200Greece Fisheries Department of Directorate of Agricultural Economy Region of Western GreecePatras26443Greece

The average values of the seasonal flesh biochemical composition(%)of the pearl oyster Pinctada imbricata radiata originated form 2 sampling sites,the gulfs of Evoikos(E)and Saronikos(S)in the Western Aegean Sea,showed that is rich in proteins(64.00±1.86-(E),64.67±2.95-(S))with low fat content(10.96±1.04-(E),11.86±1.13-(S))and carbohydrates(13.29±2.48-(E),9.94±4.32(S)).The condition index ranged from 26.16%±5.04 in the autumn in(E),to 44.73%±7.50 in the summer in(S).The meat yield varied from 20.49%±3.20%in the summer in(E)to 30.73%±3.47%in the summer in(S).Both results demonstrate the high nutritional profile of the pearl oyster,supporting its suitability as a potential new Mediterranean seafood source for human consumption.

关键词： pearl oyster Condition index Meat yield Biochemical composition Aegean

Cloning and characterization of 37 kDa laminin receptor precursor in pearl oyster,Pinctada fucata

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Progress in Natural Science:Materials International 2008年第2期18卷 233-238页

作者： Yaopeng Fu a,Liping Xie a,b,,Rongqing Zhang a,b,a Institute of Marine Biotechnology,Department of Biological Sciences and Biotechnology,Tsinghua University,Beijing 100084,China b Protein Science Laboratory of the Ministry of Education,Tsinghua University,Beijing 100084,China Institute of Marine Biotechnology Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China Protein Science Laboratory of the Ministry of Education Tsinghua University Beijing 100084 China

A 1063 bp cDNA clone encoding a putative 37 kDa laminin receptor precursor (37 kDa LRP) is isolated from the mantle tissue of pearl oyster,Pinctada fucata. The amino acid sequence predicted from the cDNA sequence is 301 residues long,with a calculated molec-ular mass of 33.5 kDa. RT-PCR analysis shows that 37 kDa LRP mRNA is especially highly expressed in the mantle while widely expressed in several tissues. In situ hybridization analysis reveals that 37 kDa LRP is expressed in the outer epithelial cells of the mantle edge,suggesting its involvement in cell proliferation and secretion in P. fucata. The identification and characterization of 37 kDa LRP in the pearl oyster will help us to further understand the signal transduction in the processes of mantle epithelial cell proliferation and tissue formation.

关键词： 37 kDa laminin receptor precursor Mantle epithelial cell pearl oyster

Cloning and Characterization of a Homologous Ca^(2+)/Calmodulin-Dependent Protein Kinase PSKH1 from pearl oyster Pinctada fucata

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Tsinghua Science and Technology 2005年第4期10卷 504-511页

作者：戴益平谢莉萍熊训浩陈蕾范为民张荣庆 Institute of Marine Biotechnology Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China Protein Science Laboratory of the Ministry of Education Tsinghua University Beijing 100084 China

Many of the effects of Ca^2＋ signaling are mediated through the Ca^2＋/calmodulin complex and its acceptors, the Ca^2＋/calmodulin-dependent protein kinases, including PSKHI. Studies of the proteins involved in the calcium metabolism in oysters will help elucidate the pearl formation mechanism. This paper describes a full-length PSKH1 cDNA isolated from pearl oyster Pinctada fucata. oyster PSKH1 shares 65% homology with human PSKH1 and 48% similarity with rat CaM kinase I in the amino acid sequence, and contains a calmodulin-binding domain. The results of semi-quantitative reverse transcription-polymerase chain reaction and in situ hybridization revealed that oyster PSKH1 mRNA is highly expressed in the outer epithelial cells of the mantle pallial and in the gill epithelial cells. These studies provide important information describing the complex Ca^2＋ signaling mechanism in oyster calcium metabolism.

关键词： PSKH1 calcium metabolism biomineralization pearl oyster Pinctada fucata Ca^2+/calmodulin-dependent protein kinases (CaMKs)

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Molecular Cloning and Distribution of a Plasma Membrane Calcium ATPase Homolog from the pearl oyster Pinctada fucata

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Tsinghua Science and Technology 2008年第4期13卷 439-446页

作者：王雪范为民谢莉萍张荣庆 Institute of Marine Biotechnology Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China Institute of Marine Biotechnology Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China Protein Science Laboratory of the Ministry of Education Tsinghua University Beijing 100084 China

Plasma membrane calcium ATPase （PMCA） plays a critical role in transporting Ca^2＋ out of the cytosol across the plasma membrane which is essential both in keeping intracellular Ca^2＋ homeostasis and in biomineralization. In this paper we cloned and localized a gene encoding PMCA from the pearl oyster Pinctada fucata. This PMCA shares similarity with other published PMCAs within the functional domains. Reverse transcription-polymerase chain reaction analysis shows that it is expressed ubiquitously. Furthermore, in situ hybridization reveals that it is expressed in the inner epithelial cells of the outer fold and in the outer epithelial cells of the middle fold, as well as the edge near the shell, which suggests that PMCA may be involved in calcified layer formation. The identification and characterization of oyster PMCA can help to further understand the structural and functional properties of molluscan PMCA, as well as the mechanism of maintaining Ca^2＋ homeostasis and the mechanism of mineralization in pearl oyster.

关键词： biomineralization Ca^2+ homeostasis pearl oyster plasma membrane calcium ATPase Pinctada fucata

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Partial Purification and Properties of an Acid Phosphatase from pearl oyster Pinctada Fucata

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Tsinghua Science and Technology 2003年第4期8卷 476-480页

作者：柴云峰谢莉萍张荣庆 Center for Ocean Science and Engineering Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China

Acid phosphatases (ACPs) are marker enzymes for the detection of lysosomes in cell fractions. However, ACPs in sea creatures are less studied than those on land. An acid phosphatase was partially purified from pearl oyster Pinctada fucata by chromatography on Sephadex G 150 and Con A Sepharose 4B. The specific activity was 1719 U·mg -1 and with optimum pH (5.0) and temperature (60℃). The enzyme was strongly inhibited competitively by product analog WO 3- 4 and MoO 3- 4, but less inhibited by product analog AsO 3- 4. The enzyme could also be strongly inhibited by heavy metal ions, such as Ag + and Cu 2+ , but was not affected by Pb 2+ . High concentrations of ethanol (64%) and NaF (10 -3 mol·L -1 ) could inhibit the enzyme while low concentration of NaF (<10 -4 mol·L -1 ) could slightly activate the enzyme. Other haloids (Cl -, Br -, I -) and EDTA did not have any effect on this enzyme, while tartrate and some chemical modification reagents (bromoacetic acid, formaldehyde and dithiothreitol) could inhibit the enzyme. It is concluded that the properties of the enzyme are different from many fresh water mollusks.

关键词： acid phosphatase pearl oyster Pinctada fucata kinetics

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Development of Expressed Sequence Tags from the pearl oyster,Pinctada martensii Dunker

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Development of Expressed Sequence Tags from the pearl oyster...

Comparison of food sources of Pinctada fucata,***,*** and Pteria penguin using lipid and fatty acid biomarkers

中国动物学会、中国海洋湖沼学会贝类学会分会第十四次学会研讨会

作者：王爱民 The Ocean College Hainan UniversityKey Laboratory of Tropic Biological Resources Ministry of EducationHainan Key Laboratory of Tropical Hydrobiological Technology

The pearl oyster,Pinctada martensii,is the primary species used for the aquaculture production of marine pearls in China and *** tools and resources are needed to study the genome of this species and to understand the molecular basis of development,growth, host-defense response,pearl formation and other important *** this study,we developed a set of expressed sequence tags(ESTs)for *** constructed a cDNA from adult tissues and sequenced 7120 *** analysis identified 788 contigs(covering 5769 ESTs)and 1351 singletons,yielding a total of 2139 unique *** these unique genes,only 935 had significant (E-value≤0.005)hits in GenBank and the remaining 1204(56.29%?)were *** of the known genes are related to cellular structure,protein binding and metabolic *** host-defense genes(108)were identified including C-type lectin,ferritin,polyubiquitin,proteases, protease inhibitors,scavenger receptors,heat shock proteins and RAS *** EST sequences developed in this study provide a valuable resource for future efforts on gene identification,marker development and studies on molecular mechanism of host-defense in pearl oysters.

关键词： expressed sequence tags(ESTs) genetic markers host-defense pearl oyster Pinctada martensii aquaculture

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Comparison of food sources of Pinctada fucata,***,*** and Pt...