Cloning and characterization of 37 kDa laminin receptor precursor in pearl oyster,Pinctada fucata

作     者：Yaopeng Fu a,Liping Xie a,b,,Rongqing Zhang a,b,a Institute of Marine Biotechnology,Department of Biological Sciences and Biotechnology,Tsinghua University,Beijing 100084,China b Protein Science Laboratory of the Ministry of Education,Tsinghua University,Beijing 100084,China 

作者机构：Institute of Marine Biotechnology Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China Protein Science Laboratory of the Ministry of Education Tsinghua University Beijing 100084 China 

出 版 物：《Progress in Natural Science:Materials International》 (自然科学进展·国际材料(英文))

年 卷 期：2008年第18卷第2期

页      面：233-238页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：2006AA09Z413,2006AA09Z441 National Natural Science Foundation of China,NSFC: 30530600 

主　　题：37 kDa laminin receptor precursor Mantle epithelial cell Pearl oyster 

摘      要：A 1063 bp cDNA clone encoding a putative 37 kDa laminin receptor precursor (37 kDa LRP) is isolated from the mantle tissue of pearl oyster,Pinctada fucata. The amino acid sequence predicted from the cDNA sequence is 301 residues long,with a calculated molec-ular mass of 33.5 kDa. RT-PCR analysis shows that 37 kDa LRP mRNA is especially highly expressed in the mantle while widely expressed in several tissues. In situ hybridization analysis reveals that 37 kDa LRP is expressed in the outer epithelial cells of the mantle edge,suggesting its involvement in cell proliferation and secretion in P. fucata. The identification and characterization of 37 kDa LRP in the pearl oyster will help us to further understand the signal transduction in the processes of mantle epithelial cell proliferation and tissue formation.