Molecular Cloning and Distribution of a Plasma Membrane Calcium ATPase Homolog from the Pearl Oyster Pinctada fucata

作     者：王雪 范为民 谢莉萍 张荣庆 

作者机构：Institute of Marine Biotechnology Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China Institute of Marine Biotechnology Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China Protein Science Laboratory of the Ministry of Education Tsinghua University Beijing 100084 China 

出 版 物：《Tsinghua Science and Technology》 (清华大学学报（自然科学版（英文版）)

年 卷 期：2008年第13卷第4期

页      面：439-446页

核心收录：

学科分类：0908[农学-水产] 09[农学] 

基　　金：the National Natural Science Foundation of China (Nos. 30530600  30371092  and 30221003) 

主　　题：biomineralization Ca^2+ homeostasis pearl oyster plasma membrane calcium ATPase Pinctada fucata 

摘      要：Plasma membrane calcium ATPase （PMCA） plays a critical role in transporting Ca^2＋ out of the cytosol across the plasma membrane which is essential both in keeping intracellular Ca^2＋ homeostasis and in biomineralization. In this paper we cloned and localized a gene encoding PMCA from the pearl oyster Pinctada fucata. This PMCA shares similarity with other published PMCAs within the functional domains. Reverse transcription-polymerase chain reaction analysis shows that it is expressed ubiquitously. Furthermore, in situ hybridization reveals that it is expressed in the inner epithelial cells of the outer fold and in the outer epithelial cells of the middle fold, as well as the edge near the shell, which suggests that PMCA may be involved in calcified layer formation. The identification and characterization of oyster PMCA can help to further understand the structural and functional properties of molluscan PMCA, as well as the mechanism of maintaining Ca^2＋ homeostasis and the mechanism of mineralization in pearl oyster.