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Single-wavelength-excited fluorogenic nanoprobe for accurate realtime ratiometric analysis of broad pH fluctuations in mitophagy

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Nano Research 2022年第7期15卷 6515-6521页

作者： Xin Zhang Juan Chen Jiwen Hu Anna du Rietz Xiongyu Wu Ruilong Zhang Zhongping Zhang Kajsa Uvdal Zhangjun Hu Department of Physics Chemistryand Biology(IFM)Linköping UniversityLinköping SE 58183Sweden School of Chemistry and Chemical Engineering Anhui UniversityHefei 230601China

Mitophagy has a critical role in maintaining cellular homeostasis through acidic lysosomes engulfing excess or impaired mitochondria,thereby pH fluctuation is one of the most significant indicators for tracking *** such precise pH tracking demands the fluorogenic probe that has tailored contemporaneous features,including mitochondrial-specificity,excellent biocompatibility,wide pH-sensitive range of 8.0–4.0,and especially quantitative ***,available molecular probes cannot simultaneously meet all the requirements since it is extremely difficult to integrate multiple functionalities into a single *** fully address this issue,we herein integrate two fluorogenic pH sensitive units,a mitochondria-specific block,cellpenetrating facilitator,and biocompatible segments into an elegant silica nano scaffold,which greatly ensures the applicability for real-time tracking of pH fluctuations in *** significantly,at a single wavelength excitation,the integrated pHsensitive units have spectra-distinguishable fluorescence towards alkaline and acidic pH in a broad range that covers mitochondrial and lysosomal pH,thus enabling a ratiometric analysis of pH variations during the whole *** work also provides constructive insights into the fabrication of advanced fluorescent nanoprobes for diverse biomedical applications.

关键词： mitochondrial pH mitophagy ratiometric fluorescence nanoprobe cell imaging

Synchronous detection of glutathione/hydrogen peroxide for monitoring redox status in vivo with a ratiometric upconverting nanoprobe

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Nano Research 2019年第4期12卷 931-938页

作者： Judun Zheng Yunxia Wu Da Xing Tao Zhang MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science South China Normal University Guangzhou 510631 China College of Biophotonics South China Normal University Guangzhou 510631 China

Cellular redox status presents broad implications with diverse physiological and pathological processes. Simultaneous detection of both the oxidative and reductive species of redox couples, especially the most representative pair glutathione/hydrogen peroxide (GSH/H2O2), is crucial to accurately map the cellular redox status. However, it still remains challenging to synchronously detect GSH/H2O2 in vivo due to lack of a reliable measuring tool. Herein, a ratiometric nanoprobe (UCNP-TB) possessing simultaneous delectability of GSH/H2O2 is established based on a multi-spectral upconverti ng nano phosphor (UCNP-OA) as the lumin esce nee res onance energy tran sfer (LRET) donor and two dye molecules as the acceptors, including a GSH-sensitive dye (TCG) and a H2O2-sensitive dye (BCH). With the as-prepared UCNP-TB, real-time and synchronous monitoring the variation of GSH and H2O2 in vitro and in living mice can be achieved using the ratio of the upcon versi on lumin esce nee (UCL) at 540 and 650 nm to that at 800 nm as the detecti on sign al, respectively, providi ng highly inhere nt reliability of the sensing results by self-calibrati on. Moreover, the nan oprobe is capable of mappi ng the redox status within the drug-resista nt tumor and the drug-induced hepatotoxic liver via ratiometric UCL imaging. Thus, this nan oprobe would provide a reliable tool to elucidate the redox state in vivo.

关键词： redox status glutathione hydrogen peroxide upconversion imaging nanoprobe

^(1)O_(2)-activatable Eu^(3+)-afterglow nanoprobe for highly sensitive detection of porphyria in whole blood

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Journal of Rare Earths 2022年第9期40卷 1382-1388,I0002页

作者： Jia Liu Jiamiao Yin Hang Yuan Yiyang Zhao Shihua Luo Fuyou Li Department of Chemistry State Key Laboratory of Molecular Engineering of PolymersFudan UniversityShanghai 200433China Hangzhou Foreign Languages School Hangzhou 310023China Department of Traumatology Ruijin HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200025China

High-sensitivity detection of porphyrin in blood is very important for the early diagnosis and treatment of *** on the advantages of longer luminescence lifetime and lower background interference,organic afterglow molecular porphyrin detection probes were developed,but these probes show poor water solubility and insufficient luminescence ***,we present an afterglow nanoprobe(Eu-NP)for porphyria detection in whole *** luminescent substance(europium complex)and the organic vinyl co mpound reacted with singlet oxygen(^(1)O_(2))were selected for encapsulation in polystyrene ***-NP can respond to^(1)O_(2)produced by porphyrins,and the detection signals can be obtained by the emission of the afterglow Eu^(3+)-emissive ***-NP can achieve high-sensitivity detection of porphyrin,and the lowest detection limit reaches 0.29μmol/*** porphyrin detection in whole blood samples is consistent with clinical diagnosis *** with the organic afterglow molecule,the Eu-NPs constructed in this work show a higher signal-to-noise ratio and sensitivity of porphyria detection.

关键词： Porphyria Afterglow nanoprobe Europium luminescence Whole blood detection Rare earths

Super-sensitive bifunctional nanoprobe: Self-assembly of peptide-driven nanoparticles demonstrating tumor fluorescence imaging and therapy

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Acta Pharmaceutica Sinica B 2022年第3期12卷 1473-1486页

作者： Han Xiao Rui Zhang Xiaobo Fan Xinglu Jiang Mingyuan Zou Xuejiao Yan Haiping Hao Guoqiu Wu Medical School Southeast UniversityNanjing 210009China Department of Clinical Laboratory the Fifth Affiliated Hospital of Sun Yat-sen UniversityNanjing 210009China Department of Cardiology the Affiliated Changzhou No.2 People’s Hospital of Nanjing Medical UniversityNanjing 210009China State Key Laboratory of Natural Medicines Key Lab of Drug Metabolism and PharmacokineticsChina Pharmaceutical UniversityZhuhai 519000China Center of Clinical Laboratory Medicine Zhongda HospitalSoutheast UniversityNanjing 21009China Jiangsu Provincial Key Laboratory of Critical Care Medicine Southeast UniversityChangzhou 213000China

The development of nanomedicine has recently achieved several breakthroughs in the field of cancer treatment;however,biocompatibility and targeted penetration of these nanomaterials remain as limitations,which lead to serious side effects and significantly narrow the scope of their *** self-assembly of intermediate filaments with arginine-glycine-aspartate(RGD)peptide(RGDIFP)was triggered by the hydrophobic cationic molecule 7-amino actinomycin D(7-AAD)to synthesize a bifunctional nanoparticle that could serve as a fluorescent imaging probe to visualize tumor *** designed RGD-IFP peptide possessed the ability to encapsulate 7-AAD molecules through the formation of hydrogen bonds and hydrophobic interactions by a one-step *** fluorescent nanoprobe with RGD peptide could be targeted for delivery into tumor cells and released in acidic environments such as endosomes/lysosomes,ultimately inducing cytotoxicity by arresting tumor cell cycling with inserted *** is noteworthy that the RGD-IFP/7-AAD nanoprobe tail-vein injection approach demonstrated not only high tumor-targeted imaging potential,but also potent antitumor therapeutic effects in *** proposed strategy may be used in peptide-driven bifunctional nanoparticles for precise imaging and cancer therapy.

关键词： nanoprobe 7-Amino actinomycin D Intermediate filament protein Tumor image Antitumor therapy Integrin avβ3

3,4-Dinitrobenzamide Functionalized CdTe/ZnTe Quantum Dots as a nanoprobe for Imaging Glutathione S-Transferase in Living Cells

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Chinese Journal of Chemistry 2013年第4期31卷 472-478页

作者： Jinxin Lu Yanchao Song Wen Shi Xiaohua Li Huimin Ma Beijing National Laboratory for Molecular Sciences Key Laboratory of Analytical Chemistry for Living Biosystems Institute of Chemistry Chinese Academy of Sciences Beijing 100190 China

A novel fluorescent nanoprobe for glutathione S-transferase （GST） has been developed by incorporating 3,4-dinitrobenzamide （a specific substrate of GST） onto CdTe/ZnTe quantum dots. The probe itself displays a low background signal due to the strong quenching effect of the electron-withdrawing unit of 3,4-dinitrobenzamide on the quantum dots. However, GST can efficiently catalyze the nucleophilic substitution of reduced glutathione on the p-nitro group of the nanoprobe, leading to a large fluorescence enhancement. Most notably, this enhancement shows high selectivity and sensitivity towards GST instead of the other biological substances. With this nanoprobe, a simple fluorescence imaging method for intracellular GST has been established, and its applicability has been successfully demonstrated for imaging GST in different living cells, which reveals that A549 cells express GST about 3 times higher than NIH-3T3 and Hela cells.

关键词： glutathione S-transferase fluorescence nanoprobe quantum dots bioimaging

A nitroreductase-responsive nanoprobe with homogeneous composition and high loading for preoperative non-invasive tumor imaging and intraoperative guidance

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Chinese Chemical Letters 2024年第7期35卷 313-318页

作者： Jingqi Xin Shupeng Han Meichen Zheng Chenfeng Xu Zhongxi Huang Bin Wang Changmin Yu Feifei An Yu Ren Institute of Medical Engineering Department of BiophysicsSchool of Basic Medical ScienceHealth Science CenterXi'an Jiaotong UniversityXi'an 710061China School of Public Health Health Science CenterXi'an Jiaotong UniversityXi'an 710061China Key Laboratory of Flexible Electronics(KLOFE)&Institute of Advanced Materials(IAM) Nanjing Tech UniversityNanjing 211816China Department of Breast Surgery the First Affiliated Hospital of Xi'an Jiaotong UniversityXi'an 710061China

Tumor microenvironment(TME)-activatable probes have been proven to effectively increase signal-tobackground ratios(SBRs)and improve the success rate of complete tumor ***,many fluorescence probes have to be loaded into a nanocarrier for tumor targeted delivery,which consequently encounters poor drug loading,heterogeneous composition and non-encapsulated drug aggregates occurred during nanoformulation ***,a nitroreductase(NTR)-activated“OFF-ON”near-infrared fluorescence nanoprobe,named Nano Bodipy,was synthesized by the spontaneous self-assembling of NTRresponsive dye-polyethylene glycol(PEG)amphiphilic polymer in *** NTR-responsive dye acted as the hydrophobic segment in the amphiphilic polymer,yielding a homogeneous composition and a high loading of 12.2 wt%(according to calculation)in the synthesized Nano *** synthesized Nano Bodipy can efficiently accumulate in tumors via the enhanced permeability and retention(EPR)effect,enabling non-invasive tumor-targeted fluorescence imaging and guiding complete tumor *** the synthesized Nano Bodipy entered the tumor cells,they dissociated and were activated by overexpressed *** the real-time fluorescence guide of Nano Bodipy,complete tumor resection surgery was performed successfully.

关键词： Nitroreductase Tumor diagnosis Intraoperative guidance Self-assembly nanoprobe

FRET-based ratiometric fluorescent detection of arginine in mitochondrion with a hybrid nanoprobe

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Chinese Chemical Letters 2020年第2期31卷 443-446页

作者： Yueyue Li Yanan Ban Ruihui Wang Zheng Wang Zhanxian Li Chenjie Fang Mingming Yu College of Chemistry and Molecular Engineering Zhengzhou UniversityZhengzhou 450001China School of Pharmaceutical Sciences Capital Medical UniversityBeijing 100069China

A ratiometric fluorescent hybrid nanoprobe CDs-1 for arginine(Arg),exhibiting high sensitivity(the limit of detection,LOD,being 6.5×10^-8 mol/L) and excellent selectivity and anti-interference ability,was fabricated through fluorescence resonance energy transfer(FRET) and the electrostatic attraction between positively-charged hemicyanine molecules and negatively-charged carbon dots(CDs).Arg can be quantitatively detected in the concentration range from 6.0×10^-5 mol/L to 2.7×10^-4 mol/***,due to its ability to target mitochondrion and low cytotoxicity,intracellular Arg was succes s fully tracked through ratiometric fluorescence imaging.

关键词： FRET Ratiometric Arg Mitochondrion nanoprobe Cell imaging

A Near-Infrared BODIPY-Loaded nanoprobe for Targeted Imaging of Hypoxia Cardiomyocytes in Myocardial Ischemia

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A Near-Infrared BODIPY-Loaded Nanoprobe for Targeted Imaging...

FRET Spectral Unmixing:A Ratiometric Fluorescent nanoprobe for Hypochlorite

第十届全国化学生物学学术会议

作者： Yunshi Fan Bo-Yang Yu Jiangwei Tian Jiangsu Key Laboratory of TCM Evaluation and Translational Research Department of Complex Prescription of TCMChina Pharmaceutical University

Hypoxia, which results from an inadequate oxygen supply in the body, has been associated with various diseases, such as tumors, cardiac ischemia, stroke and vascular diseases.1 Therefore, the development of efficient diagnostic agents with the capabilities like the accurate detection of hypoxic level will be helpful to estimate the degree of ischemia and subsequently guide more effective personalized treatment. Based on the researches that the nitro functional group has excellent properties and as one of the candidates for detecting hypoxia,2 we developed a near-infrared BODIPY-loaded nanoprobe functionalized with targeting ligand in which a large fluorescence increase is triggered by the reduction of the nitro. After myocardial infarction（MI）, the nanoprobe could specifically recognize myocardial cells and were selectively taken up by myocardial cells, where they can release hypoxia-sensitive probe and get ―turn-on‖ fluorescent signal. The nanoprobe can be used for fluorescence imaging of hypoxic cardiomyocytes and real-time monitoring of ischemia in the heart. This work presents a simple but novelty protocol with great potential in myocardial hypoxia and ischemia monitoring.

关键词： Myocardial ischemia Hypoxia BODIPY nanoprobe

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FRET Spectral Unmixing:A Ratiometric Fluorescent Nanoprobe f...