3,4-Dinitrobenzamide Functionalized CdTe/ZnTe Quantum Dots as a Nanoprobe for Imaging Glutathione S-Transferase in Living Cells

作     者：Jinxin Lu Yanchao Song Wen Shi Xiaohua Li Huimin Ma 

作者机构：Beijing National Laboratory for Molecular Sciences Key Laboratory of Analytical Chemistry for Living Biosystems Institute of Chemistry Chinese Academy of Sciences Beijing 100190 China 

出 版 物：《Chinese Journal of Chemistry》 (中国化学（英文版）)

年 卷 期：2013年第31卷第4期

页      面：472-478页

核心收录：

学科分类：080903[工学-微电子学与固体电子学] 0809[工学-电子科学与技术（可授工学、理学学位）] 07[理学] 070205[理学-凝聚态物理] 08[工学] 09[农学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0904[农学-植物保护] 090402[农学-农业昆虫与害虫防治] 0702[理学-物理学] 

基　　金：中国科学院项目 国家科技部项目 国家自然科学基金 

主　　题：glutathione S-transferase fluorescence nanoprobe quantum dots bioimaging 

摘      要：A novel fluorescent nanoprobe for glutathione S-transferase （GST） has been developed by incorporating 3,4-dinitrobenzamide （a specific substrate of GST） onto CdTe/ZnTe quantum dots. The probe itself displays a low background signal due to the strong quenching effect of the electron-withdrawing unit of 3,4-dinitrobenzamide on the quantum dots. However, GST can efficiently catalyze the nucleophilic substitution of reduced glutathione on the p-nitro group of the nanoprobe, leading to a large fluorescence enhancement. Most notably, this enhancement shows high selectivity and sensitivity towards GST instead of the other biological substances. With this nanoprobe, a simple fluorescence imaging method for intracellular GST has been established, and its applicability has been successfully demonstrated for imaging GST in different living cells, which reveals that A549 cells express GST about 3 times higher than NIH-3T3 and Hela cells.