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molecular characteristics of New Wheat Starch and Its Digestion Behaviours

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Journal of Integrative Agriculture 2014年第5期13卷 1146-1153页

作者： ZHOU Zhong-kai HUA Ze-tian YANG Yan ZHENG Pai-yun ZHANG Yan CHEN Xiao-shan Key Laboratory of Food Nutrition and Safety Ministry of Education/Tianjin University of Science and Technology China National Japonica Rice R&D Center

In order to understand the effect of starch molecular characteristics on the gel structure, which subsequently influence the gel digestion behaviours, three wheat starches, control （conventional wheat starch）, two new wheat cultivars with different genetic backgrounds （by knocking out SBE IIb and SBE IIa, respectively） were used in this study. In comparison with control, slight differences in the morphology of the starch granules of new wheat 1 were observed, whereas the starch granules of new wheat 2 had irregular shapes both for A-type granules and B-type granules. Starch molecular weight size was determined by SE-HPLC, and the results indicate that there was a subtle increase in the amylose content in the starch of new wheat 1 compared to that of control. The starch of new wheat 2 had the highest amylose content, and the molecular weight （MW） of its amylopectin was the lowest among the three starches. Fourier transform infrared spectroscopy （FTIR） was employed to investigate starch gel structure and the results suggest that the molecules of starch gel from new wheat 2 are more likely to re-associate to form an organized conformation. The digestion behaviours of the three starch gels were measured using a mixture of pancreatin a-amylase and amyloglucosidase. The results indicated that the starch gels of control and new wheat 1 had very high digestibility of 91.7 and 91.9%, respectively, whereas the digestibility of wheat 2 starch gel was only 36.2%. In comparison with the digestion curve pattems of control and new wheat 1 starch gels, the new wheat 2 exhibited a much lower initial velocity. These results indicated that the molecules in the starch of new wheat 2 are more readily to re-associate to form an organized structure during gel formation because of its unique molecular characteristics.

关键词： wheat starch starch gels molecular characteristics digestion

molecular characteristics and antimicrobial susceptibility of Staphylococcus aureus among children with respiratory tract infections in southwest China

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World Journal of Pediatrics 2020年第3期16卷 284-292页

作者： Chen Sun Qing Wang Wen-Ting Li De-Nian Wen Chang-Hui Chen Xin Yang Wei Shi Qing-Hong Meng Kai-Hu Yao Su-Yun Qian Pediatric Intensive Care Unit Beijing Children's HospitalCapital Medical UniversityNational Center for Children's HealthNanLiShi Road 56Xicheng DistrictBeijing 100045China Key Laboratory of Major Diseases in Children National Key Discipline of Pediatrics(Capital Medical University)National Clinical Research Center for Respiratory DiseasesBeijing Key Laboratory of Pediatric Respiratory Infection DiseasesBeijing Pediatric Research InstituteBeijing Children's HospitalCapital Medical UniversityNational Center for Children's HealthBeijing 100045China Respiratory Department Qilu Children's HospitalShandong UniversityJinan 250022China Department of Pediatrics People's Hospital of Zhongjiang CountyNo.96 Da-bei StreetDeyang 618100China Youyang Hospital The First Affiliated Hospital of Chongqing Medical UniversityPeople's Hospital of Chongqing Youyang CountyChongqing 409899China

Background The molecular characteristics and antimicrobial susceptibility of Staphylococcus aureus (***) in general pediatric wards and county-level hospitals were rarely reported in *** Staphylococcus aureus was isolated from children hospitalized with respiratory tract infection (RTI) in Zhongjiang and Youyang counties in *** isolates were typed by multilocus sequence,staphylococcal protein A,accessory gene regulator (agr),and staphylococcal cassette chromosome mec [SCCmec,for methicillin-resistant *** (MRSA) only].Polymerase chain reaction was used to screen 21 super-antigen (SAg) genes and panton-valentine leukocidin (pvl).Antimicrobial susceptibility testing was performed by E *** A total of 2136 children were ***,125 (5.9%) children carried ***,among which MRSA accounted for 42.4%.ST59-SCCmec type Ⅳ-t437-agr group Ⅰ (58.5%) was the most prevalent genotype in MRSA,and ST188-t189-agr group Ⅰ (22.2%) was the top genotype in methicillin-sensitive *** (MSSA).The pvl carriage rate in MRSA and MSSA was 15.1% and 9.7%,respectively (P =0.4112).About 96.8% of *** isolates were positive for at least one SAg *** most common SAg gene profile in the dominant ST59 clone was seb-sek-seq (42.8%).All *** isolates were resistant to penicillin and erythromycin (minimum inhibitory concentration 90 was > 32 and 256 mg/L to penicillin and erythromycin,respectively),but usually susceptible to other tested non-β-lactam *** Staphylococcus aureus and MRSA were detected with a high frequency in children with RTI in county-level hospitals of ***59-SCCmec type Ⅳ-t437-agr group Ⅰ was the dominant MRSA *** *** isolates exhibited high resistance to penicillin and erythromycin.

关键词： Antimicrobial susceptibility Chinese children molecular characteristics Staphylococcus aureus

molecular characteristics and structure–activity relationships of food-derived bioactive peptides

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Journal of Integrative Agriculture 2021年第9期20卷 2313-2332页

作者： YANG Fu-jia CHEN Xu HUANG Mu-chen YANG Qian CAI Xi-xi CHEN Xuan DU Ming HUANG Jian-lian WANG Shao-yun Key Laboratory of Refrigeration and Conditioning Aquatic Products Processing of Ministry of Agriculture and Rural Affairs Xiamen 361022P.R.China College of Biological Science and Technology Fuzhou UniversityFuzhou 350108P.R.China College of Chemical Engineering Fuzhou UniversityFuzhou 350108P.R.China School of Food Science and Technology Dalian Polytechnic UniversityDalian 116034P.R.China Fujian Anjoy Foods Co. Ltd.Xiamen 361022P.R.China

Peptides are functional active fragments of proteins which can provide nutrients needed for human growth and development,and they also have unique physiological activity characteristics relative to *** peptides contain a great deal of development *** specifically,food-derived bioactive peptides have the advantages of a wide variety of sources,unique structures,high efficiency and safety,so they have broad development *** review provides an overview of the current advances regarding the preparation,functional characteristics,and structure–activity relationships of food-derived bioactive ***,the prospects for the future development and application of food-derived bioactive peptides are *** review may provide a better understanding of foodderived bioactive peptides,and some constructive inspirations for further research and applications in the food industry.

关键词： food-derived proteins bioactive peptides molecular characteristics structure–activity relationship

molecular characteristics of S1 Gene of Infectious Bronchitis Virus Isolated from Chicken Proventriculus

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Agricultural Sciences in China 2003年第1期2卷 107-112页

作者： CHENG Li-qin, ZHOU Ji-yong, John Dikki, SHEN Xing-yan, CHEN Ji-gang and ZHANG De-yongInstitute of Preventive Veterinary Medicine , College of Animal Sciences , Zhejiang University , Hangzhou 310029 , P.R.China Institute of Preventive Veterinary Medicine College of Animal SciencesZhejiang University Hangzhou 310029 P. R. China

Infectious bronchitis virus was isolated from swollen proventriculi of clinically ill chicken. The suspected virus samples (2/97, 3/97, 1/98) were adapted in SPF chicken embryos for virus isolation and identification. All the virus isolates were able to agglutinate chicken erythrocytes after treatment with trypsin, and interfer with the reproduction of Newcastle disease virus in chicken embryos, and have low antigenic relat-edness values with reference positive IBV. The isolates 2/97, 3/97, 1/98 RNAs extracted from the allantoic fluid of inoculated embryonated eggs were converted to cDNA by reverse transcription with 3'-primer of S1 gene of (IBV). Polymerase chain reaction (PCR) was performed with two primers which span the S1 gene. Amplified product of 1. 93 kb was subjected to EcoR I and BamH I digestion and the fragments obtained were the same as expected size. The PCR product was ligated to pBlueScript-SK ( + ) vector, and its nucleotide sequence was determined by the dideoxy-mediated chain termination method. Nucleotide sequence analysis showed 73. 6 - 99. 7% homology between the isolated IBV and the IBV strains in GenBank. The homology of amino acid was 71. 4 - 99.4%.

关键词： Chicken Infectious bronchitis virus S1 gene molecular characteristics

molecular characteristics and Phylogenetic Analysis of N Gene of Human Derived Rabies Virus

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Biomedical and Environmental Sciences 2011年第4期24卷 431-437页

作者： CAI Liang TAO XiaoYan LIU YunZhi ZHANG Hong GAO LiDong HU ShiXiong LIU FuQiang LI Hao SHEN XinXin LIU JiaHui WANG ShiQing TANG Qing Hunan Center for Disease Control and Prevention Key Laboratory of Microbial Molecular Biology of Hunan Province Changsha 410005 Hunan China State Key Laboratory for molecular Virology and Genetic Engineering Institute for Viral Disease Control and Prevention China CDC Beijing 102206 China

Objective To investigate the relationship between the molecular characteristics and phylogenetic evolution of rabies N *** Saliva samples were collected from rabies cases,and RT-PCR was used to amplify the N gene of rabies virus with the specific *** amplifying product of RT-PCR was cloned to pUCm-T vector and transformed into *** XL1-Blue and then the blue-white selection,PCR screening and gene sequencing were carried out to identify the positive ***,ExPASy and other bioinformatics softwares were used to analyze and predict the structure and biological characteristics of the N *** The amplification product of RT-PCR was 1 353 bp,the recombinant plasmid pUCm-T/N was constructed,the whole length of the N gene open reading frame was composed of 1 353 nucleotide residues to code 450 amino acids （20 kinds）,the accession number submitted to the Genbank was HM756692,its sequence homology of nucleotides and amino acids compared with the vaccine strain CTN-1-V was 90% and 99% *** evolutionary analysis showed that the isolated strain belonged to genotype I with certain geographic *** The characteristics investigation and bioinformatics analysis of Hunan0806 N gene will provide fundament data to reveal the significance of the N gene characteristics for rabies epidemiology and its prevention control.

关键词： Rabies N gene molecular characteristics Phylogenetic

Distribution and molecular characteristics of Vibrio cholerae O1 El Tor isolates recovered in Guangdong Province,China,1961-2013

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Distribution and molecular characteristics of Vibrio cholera...

Three anti-inflammatory polysaccharides from Lonicera japonica Thunb.:insights into the structure-function relationships

2016新发传染病研究热点研讨会

作者： Baisheng Li Rongfeng Chen Duochun Wang Department of Nutrition and Food Hygiene School of Public Health and Tropical MedicineSouthern Medical University

China’s Guangdong Province is located along the same latitude as Kolkata,India and Dhaka,Bangladesh,and is also considered a source of epidemic cholera. However,molecular description and the genetic relationships between Vibrio cholerae O1 El Tor isolates in Guangdong remain unclear. In this study,381 clinical V. cholerae O1 isolates recovered from cholera cases presenting in Guangdong between 1961 and 2013 were investigated by PCR,amplicon sequencing and pulsed-field gel electrophoresis(PFGE). During this time frame,four distinct epidemic periods(1-4) were observed based on the different dominant serotype leading its epidemic,correspond to years;or time periods from/to 1961-1969,1978-1989, 1990-2000, 2001-2013, respectively. molecular analysis of representative isolates indicated that a single dominating clone was associated with each epidemic stage. All isolates from period 1 and 2 carried the typical El Tor ctx B;this allele was displaced by classical ctx B beginning in 1993. However all isolated carried the El Tor-specific toxin-coregulated pili subunit A(tcp A). Isolates were grouped into five clusters on the basis of Not I enzyme digested PFGE,and the first four clusters were associated with specific periods,cluster Ⅰ(period 1),Ⅱ(period 3),Ⅲ(period 2) and Ⅳ(period 4),respectively. While cluster V consisted of isolates from all four epidemic periods, but was most heterogeneous in appearance. Our data indicate genetic variations that shape the relationship among emerging isolates of V. cholerae O1 in Guangdong Province contribute to the 7 global pandemic.

关键词： molecular characteristics Vibrio cholerae O1 El Tor Guangdong Province China

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Food Science and Human Wellness 2024年第4期13卷 2197-2207页

作者： Yu Liu Hongjing Dong Dongxiao Sun-Waterhouse Wenwen Li Bin Zhang Jinqian Yu Zhichang Qiu Zhenjia Zheng Key Laboratory of Food Nutrition and Health in Universities of Shandong College of Food Science and EngineeringShandong Agricultural UniversityTai’an 271018China Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province Shandong Analysis and Test CenterQilu University of Technology(Shandong Academy of Sciences)Jinan 250014China School of Chemical Sciences The University of AucklandPrivate Bag 92019New Zealand

This study demonstrates the feasibility of producing three polysaccharides(neutral LJP-1,acidic LJP-2 and acidic LJP-3)with significant in vitro and in vivo anti-inflammatory activities from the flowers of Lonicera *** three polysaccharides differed in chemical composition,molecular weight(Mw)distribution,glycosidic linkage pattern,functional groups and *** exhibited excellent protective effects(in a dose-dependent manner)in lipopolysaccharide-injured RAW264.7 macrophages and Cu SO4-damaged zebrafish via reducing NO production and inhibiting the overexpressions of inflammation-related transcription factors,inflammatory proteins and cytokines in the NF-κB/MAPK signaling *** antiinflammatory effects varied owing to their different molecular characteristics and chemical ***,LJP-2 at 400μg/m L was the most ***-2 consisted mainly of→5)-α-L-Araf(1→,→4)-α-LGalp A(1→and→2)-α-L-Rhap(1→residues with terminal T-β-***,honeysuckle flowers are good sources of anti-inflammatory polysaccharides,and precise fractionation enables the production of potent antiinflammatory agents for the development of functional foods and healthcare products.

关键词： Honeysuckle polysaccharides Fractionation molecular characteristics Anti-inflammatory properties Structure-function relationship

FT-ICR mass spectrometry for molecular characterization of water-insoluble organic compounds in winter atmospheric fine particulate matters

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Journal of Environmental Sciences 2022年第1期34卷 51-60页

作者： Cuiping Ning Yuan Gao Haoran Yu Haijun Zhang Ningbo Geng Rong Cao Jiping Chen CAS Key Laboratory of Separation Sciences for Analytical Chemistry Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalianLiaoning 116023China University of Chinese Academy of Sciences Beijing 100049China

Water-insoluble organic compounds(WIOCs)are an important fraction of atmospheric fine particulate matters(PM_(2.5)),which could affect the climate system and threaten human health *** this study,molecular characterization of WIOCs in PM_(2.5) were investigated by 15 T Fourier transform ion cyclotron resonance mass spectrometry(FT-ICR MS)with atmospheric pressure photoionization(APPI)source in positive ion mode.A total of 2573 and1875 molecular formulas were identified in WIOCs extracted by dichloromethane,which were collected in hazy and normal days,*** identified molecular formulas were further classified into four major subgroups,including CH,CHN,CHO and CHNO *** compounds predominated in WIOCs,accounting for more than 60%in both *** compounds(26.6%)and CH compounds(16.1%)were the second highest subgroups in WIOCs from the hazy days and normal days,*** relative abundance and number of nitro-substituted aromatic compounds were significantly higher in hazy days than in normal *** molecular composition of WIOCs was more complex in hazy days while more aromatic compounds were identified in normal days.

关键词： PM_(2.5) WIOCs FT-ICR MS molecular characteristics Polycyclic aromatic hydrocarbon

Biochar-derived dissolved organic matters influencing bacterium characteristics during biodegradation of sulfamethoxazole and chloramphenicol under alternation of visible and avoiding light

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Biochar 2023年第1期5卷 160-174页

作者： Fang Yang Yanan Xue Yue Gao Qing Zhu Cuiping Wang Hongwen Sun Key Laboratory of Pollution Processes and Environmental Criteria Ministry of EducationTianjin Key Laboratory of Environmental Remediation and Pollution ControlCollege of Environmental Science and EngineeringNankai UniversityTianjin 300071People’s Republic of China Tianjin Engineering Center of Environmental Diagnosis and Contamination Remediation Tianjin 300071People’s Republic of China

The influence of biochar-released dissolved organic matter(BDOM)on the transcription of gene(DEG)in Pseudomonas stutzeri and Shewanella putrefacien during sulfamethoxazole(SMX)and chloramphenicol(CAP)biodegradation under visible light was investigated in this *** results indicated that BDOM components would be nutrients for bacterial amplification and growth under the culture conditions of xenon lamp irradiation and avoiding light,especially BDOM from low ***,visible light irradiation would improve the saturated fatty acid by stimulating the cell membrane of the microorganism,thus promoting the biodegradation of antibiotics through altering *** and *** reoxidative and catabolism processes and significantly inhabiting the copy number of their ***,the upregulated genes and enzymes related to SMX and CAP-metabolic and catabolic processes were enriched,which were involved in the pathways of biodegradation,further improving biodegradation *** particular,interaction network analysis between the top 100 dominant functional genes from *** and *** and the molecular types of BDOM,e.g.,CHO,CHON,and CHOS(pmolecular function showed a high positive or negative correlation with the CHO type of *** results revealed that the CHO type of BDOM affected the functional genes of molecular function,cellular component,and biological process from *** and ***,influencing the biodegradation of SMX and *** study provided an basis for BDOM playing a role in antibiotic removal from the aqueous solution using biochar combined with photobiodegradation.

关键词： Biochar-derived dissolved organic matters molecular characteristics Antibiotics degradation Transcriptome Functional genes