Molecular characteristics and antimicrobial susceptibility of Staphylococcus aureus among children with respiratory tract infections in southwest China
作者机构：Pediatric Intensive Care UnitBeijing Children's HospitalCapital Medical UniversityNational Center for Children's HealthNanLiShi Road 56Xicheng DistrictBeijing 100045China Key Laboratory of Major Diseases in ChildrenNational Key Discipline of Pediatrics(Capital Medical University)National Clinical Research Center for Respiratory DiseasesBeijing Key Laboratory of Pediatric Respiratory Infection DiseasesBeijing Pediatric Research InstituteBeijing Children's HospitalCapital Medical UniversityNational Center for Children's HealthBeijing 100045China Respiratory DepartmentQilu Children's HospitalShandong UniversityJinan 250022China Department of PediatricsPeople's Hospital of Zhongjiang CountyNo.96 Da-bei StreetDeyang 618100China Youyang HospitalThe First Affiliated Hospital of Chongqing Medical UniversityPeople's Hospital of Chongqing Youyang CountyChongqing 409899China
出 版 物：《World Journal of Pediatrics》 (世界儿科杂志（英文版）)
年 卷 期：2020年第16卷第3期
页 面：284-292页
核心收录：
学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 100202[医学-儿科学] 10[医学]
基 金：We are very grateful to the local clinical laboratory staff Ping Tang Hai-Ling Zeng in People's Hospital of Zhongjiang County and Xiao-Ping Cheng in Youyang Hospital respectively who kindly helped us in treating and storing the clinical isolates.We also thank pediatricians in the two hospitals involved in collecting the samples
主 题：Antimicrobial susceptibility Chinese children Molecular characteristics Staphylococcus aureus
摘 要：Background The molecular characteristics and antimicrobial susceptibility of Staphylococcus aureus (***) in general pediatric wards and county-level hospitals were rarely reported in *** Staphylococcus aureus was isolated from children hospitalized with respiratory tract infection (RTI) in Zhongjiang and Youyang counties in *** isolates were typed by multilocus sequence,staphylococcal protein A,accessory gene regulator (agr),and staphylococcal cassette chromosome mec [SCCmec,for methicillin-resistant *** (MRSA) only].Polymerase chain reaction was used to screen 21 super-antigen (SAg) genes and panton-valentine leukocidin (pvl).Antimicrobial susceptibility testing was performed by E *** A total of 2136 children were ***,125 (5.9%) children carried ***,among which MRSA accounted for 42.4%.ST59-SCCmec type Ⅳ-t437-agr group Ⅰ (58.5%) was the most prevalent genotype in MRSA,and ST188-t189-agr group Ⅰ (22.2%) was the top genotype in methicillin-sensitive *** (MSSA).The pvl carriage rate in MRSA and MSSA was 15.1% and 9.7%,respectively (P =0.4112).About 96.8% of *** isolates were positive for at least one SAg *** most common SAg gene profile in the dominant ST59 clone was seb-sek-seq (42.8%).All *** isolates were resistant to penicillin and erythromycin (minimum inhibitory concentration 90 was 32 and 256 mg/L to penicillin and erythromycin,respectively),but usually susceptible to other tested non-β-lactam *** Staphylococcus aureus and MRSA were detected with a high frequency in children with RTI in county-level hospitals of ***59-SCCmec type Ⅳ-t437-agr group Ⅰ was the dominant MRSA *** *** isolates exhibited high resistance to penicillin and erythromycin.
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