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mouse

英语辅导（初二年级） 2002年第1期 8-8页

作者：蔡磊江苏兴化市徐扬乡兴倪学校

我们都知道mouse是“老鼠”的意思，其复数是mice。鼠属于啮齿类哺乳动物，种类很多，各有其名，但一般通称为鼠。在英美国家里，居民区内所见到的体形较小的老鼠叫mouse，体形较大的叫rat。

关键词： mouse mice rat 初二英语词汇

Towards estimating computer users＇ mood from interaction behaviour with keyboard and mouse

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Frontiers of Computer Science 2013年第6期7卷 943-954页

作者： Iflikhar Ahmed KHAN Willem-Paul BRINKMAN Robert HIERONS Department of Computer Software Engineering University of Engineering & Technology Mardan 23200 Pakistan Interactive Intelligence Group Delft University of Technology Delft 2628B the Netherlands Department of Information Systems and Computing Brunel University West London UB83PH UK

The purpose of this exploratory research was to study the relationship between the mood of computer users and their use of keyboard and mouse to examine the possibility of creating a generic or individualized mood measure. To examine this, a field study （n = 26） and a controlled study （n = 16） were conducted. In the field study, interaction data and self-reported mood measurements were collected during normal PC use over several days. In the controlled study, participants worked on a programming task while listening to high or low arousing background music. Besides subjective mood measurement, galvanic skin response （GSR） data was also collected. Results found no generic relationship between the interaction data and the mood data. However, the re- suits of the studies found significant average correlations be- tween mood measurement and personalized regression models based on keyboard and mouse interaction data. Together the results suggest that individualized mood prediction is pos- sible from interaction behaviour with keyboard and mouse.

关键词： keyboard mouse interaction mood measure computer users programming

The second polar body contributes to the fate asymmetry in the mouse embryo

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National Science Review 2022年第7期9卷 32-42页

作者： Hongbin Jin Yang Han Huasong Wang J.Xiao He Li Weimin Shen Lin Zhang Luxi Chen Shunji Jia Ping Yuan Hui Chen Anming Meng Laboratory of Molecular Developmental Biology State Key Laboratory of Membrane BiologyTsinghua-Peking Center for Life Sciences School of Life SciencesTsinghua University Center for Reproductive Genetics and Reproductive Medicine Sun Yat-sen Memorial HospitalSun Yat-sen University Guangzhou Laboratory

The polar bodies(PBs) are extruded microcells during oocyte meiosis and generally regarded as inessentials for embryonic ***,PBs have been widelyused as important materials for pre-implantation genetic diagnosis in *** we report that the second PB(PB2) in the mouse zygote may play roles in cell-fate specification and post-implantation development.A subset of mRNAs encoding pluripotency-related factors are enriched in *** proteins maybe synthesized in PB2 after fertilization and transport from PB2 to the zygote before the two-cell *** PB2-attached blastomere(pbB) at the two-cell stage,compared to the other blastomere(npbB),likely contributes more descendants to the inner cell mass(ICM) lineage in the *** of PB2 from the zygote or transient blockage of material exchange between PB2 and the zygote by nocodazole treatment appears to cause a loss of the ICM fate bias of ***2 removal or nocodazole treatment also results in abnormal post-implantation *** of PB2 lysate into pbB of PB2-removed two-cell-stage embryos may reset the cell-fate preference and rescue post-implantation *** data collectively suggest that PB2 would demarcate the earliest cell-fate asymmetry of the mouse zygote and be required for post-implantation development.

关键词： mouse embryo polar body cell fate asymmetry

mouse Karyotype Obtained by Combining DAPI Staining with Image Analysis

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Wuhan University Journal of Natural Sciences 2006年第2期11卷 441-446页

作者： DAI Xiaohua YANG Guangxu LIU Jingyu SONG Yunchun College of Life Science Henan University Kaifeng 475001 Henan China Department of Life Science Western Chongqing University Chongqing 402168 Siehuan China The Key l.aboratory of the Ministry of Education of Plant Developmental Biology Wuhan University Wuhan 430072 Hubei China

In this study, mitotic metaphase chromosomes in mouse were identified by a new chromosome fluorescence banding technique combining DAPI staining with image analysis. Clear 4＇, 6-diamidino-2-phenylindole （DAPI） multiple bands like （J-hands could be produced in mouse. The Meta- Morph software was then used to generate linescans of pixel intensity for the banded chromosomes from short arm to long arm. These linescans were sufficient not only to identify each individual chromosome but also analyze the physical sites of bands in chromosome. Based on the results, the clear and accurate karyotype of mouse metaphase chromosomes was established. The technique is therefore considered to he a new method for cytological studies of mouse.

关键词： mouse 4 ', 6 ditlmidino-2-phenylindole (DA-Pl) fluorescence staining image analysis karyotype

mouse models of colorectal cancer: Past, present and future perspectives

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World Journal of Gastroenterology 2020年第13期26卷 1394-1426页

作者： Florian Bürtin Christina S Mullins Michael Linnebacher Department of General VisceralVascular and Transplantation SurgeryUniversity Medical Center RostockUniversity of RostockRostock 18057Germany Department of Thoracic Surgery University Medical Center RostockUniversity of RostockRostock 18057Germany Molecular Oncology and Immunotherapy Department of GeneralVisceralVascular and Transplantation SurgeryUniversity Medical Center RostockRostock 18057Germany

Colorectal cancer(CRC)is the third most common diagnosed malignancy among both sexes in the United States as well as in the European *** the incidence and mortality rates in western,high developed countries are declining,reflecting the success of screening programs and improved treatment regimen,a rise of the overall global CRC burden can be observed due to lifestyle changes paralleling an increasing human development *** a growing insight into the biology of CRC and many therapeutic improvements in the recent decades,preclinical in vivo models are still indispensable for the development of new treatment *** the development of carcinogen-induced rodent models for CRC more than 80 years ago,a plethora of animal models has been established to study colon cancer *** tenuous invasiveness and metastatic behavior,these models are useful for chemoprevention studies and to evaluate colitis-related *** engineered mouse models(GEMM)mirror the pathogenesis of sporadic as well as inherited CRC depending on the specific molecular pathways activated or *** the vast majority of CRC GEMM lack invasiveness,metastasis and tumor heterogeneity,they still have proven useful for examination of the tumor microenvironment as well as systemic immune responses;thus,supporting development of new therapeutic *** of metastatic disease by orthotopic injection of CRC cell lines is possible,but the so generated models lack genetic diversity and the number of suited cell lines is very ***-derived xenografts,in contrast,maintain the pathological and molecular characteristics of the individual patient's CRC after subcutaneous implantation into immunodeficient mice and are therefore most reliable for preclinical drug development–even in comparison to GEMM or cell line-based ***,subcutaneous patient-derived xenograft models are less suitable for studying most aspects of the tumor micr

关键词： Colorectal cancer mouse models Patient-derived xenografts Carcinogen-induced models Genetically engineered mouse models Preclinical drug development

Differential expression of glial cell line-derived neurotrophic factor splice variants in the mouse brain

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Neural Regeneration Research 2020年第2期15卷 270-276页

作者： Xiao-He Gu Heng Li Lin Zhang Tao He Xiang Chai He Wei Dian-Shuai Gao Department of Anatomy and Neurobiology Xuzhou Medical UniversityXuzhouJiangsu ProvinceChina Department of Neurosurgery First Affiliated Hospital of Soochow UniversitySuzhouJiangsu ProvinceChina

Glial cell line-derived neurotrophic factor(GDNF) plays a critical role in neuronal survival and function. GDNF has two major splice variants in the brain,α-pro-GDNF and β-pro-GDNF, and both isoforms have strong neuroprotective effects on dopamine neurons. However, the expression of the GDNF splice variants in dopaminergic neurons in the brain remains unclear. Therefore, in this study, we investigated the mRNA and protein expression of α-and β-pro-GDNF in the mouse brain by real-time quantitative polymerase chain reaction, using splice variant-specific primers, and western blot analysis. At the mRNA level,β-pro-GDNF expression was significantly greater than that of α-pro-GDNF in the mouse brain. In contrast, at the protein level,α-pro-GDNF expression was markedly greater than that of β-pro-GDNF. To clarify the mechanism underlying this inverse relationship in mRNA and protein expression levels of the GDNF splice variants, we analyzed the expression of sorting protein-related receptor with A-type repeats(SorLA) by real-time quantitative polymerase chain reaction. At the mRNA level, SorLA was positively associated with β-pro-GDNF expression, but not with α-pro-GDNF expression. This suggests that the differential expression of α-and β-pro-GDNF in the mouse brain is related to SorLA expression. As a sorting protein, SorLA could contribute to the inverse relationship among the mRNA and protein levels of the GDNF isoforms. This study was approved by the Animal Ethics Committee of Xuzhou Medical University, China on July 14, 2016.

关键词： Δ78 locus brain region dopaminergic neurons glial cell line-derived neurotrophic factor mouse brain precursor protein α-pro-GDNF β-pro-GDNF sorting protein-related receptor with A-type repeats splice variants

The Schlager mouse as a model of altered retinal phenotype

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Neural Regeneration Research 2020年第3期15卷 512-518页

作者： Lakshini Y.Herat Aaron L.Magno Márcio G.Kiuchi Kristy L.Jackson Revathy Carnagarin Geoffrey A.Head Markus P.Schlaich Vance B.Matthews Dobney Hypertension Centre School of Biomedical Science-Royal Perth Hospital UnitUniversity of Western AustraliaPerthAustralia Research Centre Royal Perth HospitalPerthAustralia Dobney Hypertension Centre School of Medicine-Royal Perth Hospital UnitUniversity of Western AustraliaPerthAustralia Neuropharmacology Laboratory Baker Heart and Diabetes InstituteMelbourneAustralia Department of Cardiology and Department of Nephrology Royal Perth HospitalPerthAustralia

Hypertension is a risk factor for a large number of vision-threatening eye *** this study,we investigated for the first time the retinal neural structure of the hypertensive BPH/2J mouse(Schlager mouse)and compared it to its control counterpart,the normotensive BPN/3J *** BPH/2J mouse is a selectively inbred mouse strain that develops chronic hypertension due to elevated sympathetic nervous system *** compared to the BPN/3J strain,the hypertensive BPH/2J mice showed a complete loss of outer layers of the neural retina at 21 weeks of age,which was indicative of a severe vision-threatening disease potentially caused by *** elucidate whether the retinal neural phenotype in the BPH/2J strain was attributed to increased BP,we investigated the neural retina of both BPN/3J and BPH/2J mice at 4 weeks of *** preliminary results showed for the first time that the BPH/2J strain develops severe retinal neural damage at a young *** findings suggest that the retinal phenotype in the BPH/2J mouse is possibly due to elevated blood pressure and may be contributed by an early onset spontaneous mutation which is yet to be identified or a congenital defect occurring in this *** characterization of the BPH/2J mouse strain is likely to i)elucidate gene defects underlying retinal disease;ii)understand mechanisms leading to neural retinal disease and iii)permit testing of molecules for translational research to interfere with the progression of retinal *** animal experiments were performed with the approval of the Royal Perth Hospital Animal Ethics Committee(R535/17-18)on June 1,2017.

关键词： blood pressure eye hypertension mice neural regeneration retina Schlager mouse sympathetic nervous system

Comparative proteomes change and possible role in different pathways of micro RNA-21a-5p in a mouse model of spinal cord injury

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Neural Regeneration Research 2020年第6期15卷 1102-1110页

作者： Almaghalsa-Ziad Mohammed Hong-Xia Du Hong-Liang Song Wei-Ming Gong Bin Ning Tang-Hong Jia Department of Spinal Surgery Jinan Central Hospital Affiliated to Shandong University

Our previous study found that microRNA-21 a-5 p(miR-21 a-5 p)knockdown could improve the recovery of motor function after spinal cord injury in a mouse model,but the precise molecular mechanism remains poorly *** this study,a modified Allen's weight drop was used to establish a mouse model of spinal cord injury.A proteomics approach was used to understand the role of differential protein expression with miR-21 a-5 p knockdown,using a mouse model of spinal cord injury without gene knockout as a negative control *** found that after introducing miR-21 a-5 p knockdown,proteins that played an essential role in the regulation of inflammatory processes,cell protection against oxidative stress,cell redox homeostasis,and cell maintenance were upregulated compared with the negative control *** Encyclopedia of Genes and Genomes pathway enrichment analysis identified enriched pathways in both groups,such as the oxidative phosphorylation pathway,which is relevant to Parkinson's disease,Huntington's disease,Alzheimer's disease,and cardiac muscle *** also found that miR-21 a-5 p could be a potential biomarker for amyotrophic lateral sclerosis,as miR-21 a-5 p becomes deregulated in this *** results indicate successful detection of some important proteins that play potential roles in spinal cord *** the relationship between these proteins and the recovery of spinal cord injury will provide a reference for future research of spinal cord injury *** experimental procedures and protocols were approved by the Experimental Animal Ethics Committee of Shandong University of China on March 5,2014.

关键词： bioinformatics biomarker inflammation micro RNA mitochondria mouse pathway analysis proteomics spinal cord injury stathmin

Single-cell transcriptomic atlas of mouse cochlear aging

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Protein & Cell 2023年第3期14卷 180-201页

作者： Guoqiang Sun Yandong Zheng Xiaolong Fu Weiqi Zhang Jie Ren Shuai Ma Shuhui Sun Xiaojuan He QiaoranWang zhejun ji Fang Cheng KaowenYan Ziyi Liu Juan Carloszpisuaemonte Jing Qu Si Wang Renjie Chai Guang-Hui Liu State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China State Key Laboratory of Bioelectronics Jiangsu Province High-Tech Key Laboratory for Bio-Medical ResearchSchool of Life Sciences and TechnologySoutheast UniversityNanjing 211189China Advanced Innovation Center for Human Brain Protection National Clinical Research Center for Geriatric DisordersXuanwu Hospital Capital Medical University.Beijing100053China CAS Key Laboratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of SciencesBeijing 100101China National Laboratory of Biomacromolecules CAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China Aging Translational Medicine Center International Center for Aging and CancerBeijing Municipal Geriatric Medical Research CenterXuanwu HospitalCapital MedicalUniversityBeijing100053China University of Chinese Academy of Sciences Beijing 100049China Institute for Stem Cell and Regeneration CASBeijing 100101China China National Center for Bioinformation Beijing 100101China Beijing Institute for Stem Cell and Regenerative Medicine Beijing 100101China The Fifth People's Hospital of Chongqing Chongqing 400062China Gene Expression Laboratory Salk Institute for Biological StudiesLa JollaCA 92037USA Co-Innovation Center of Neuroregeneration Nantong UniversityNantong 226001China Shandong Provincial Hospital and School of Laboratory Animal Science Shandong First Medical UniversityJinan 250000China

Progressive functional deterioration in the cochlea is associated with age-related hearing loss(ARHL).However,the cellular and molecular basis underlying cochlear aging remains largely ***,we established a dynamic single-cell transcriptomic landscape of mouse cochlear aging,in which we characterized aging-associated transcriptomic changes in 27 different cochlear cell types across five different time ***,our analysis pinpoints loss of proteostasis and elevated apoptosis as the hallmark features of cochlear aging,highlights unexpected age-related transcriptional fluctuations in intermediate ceils localized in the stria vascularis(SV)and demonstrates that upregulation of endoplasmic reticulum(ER)chaperon protein HSP90AA1 mitigates ER stress-induced damages associated with *** work suggests that targeting unfolded protein response pathways may help alleviate aging-related sVatrophyand hencedelay theprogressionofARHL.

关键词： single-cell transcriptomic atlas mouse cochlea aging