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Journal of Integrative Agriculture 2020年第10期19卷 2597-2601页

作者： CUI Xue-jin ZENG Chun-hua LIU Ke-hong TENG Cai-ling ZHOU Chang-yong WANG Xue-feng National Citrus Engineering Research Center Citrus Research InstituteChinese Academy of Agricultural Sciences/Southwest UniversityChongqing 400712P.R.China

An active miniature inverted-repeat transposable element(MITE),MCL as-A,was previously identified from'Candidatus Liberibacter asiaticus'known to be associated with citrus Huanglongbing(HL B,yellow shoot disease).To explore the recent transposition status of MCLas-A,389'Ca.L.asiaticus'strains collected from nine regions in China were amplifed using a specific primer set and three representative'Ca.L.asiaticus'strains were analyzed by next-generation sequencing(NGS)approach.PCR and genomic analysis showed that the entire MCLas A was only present in 1.80%(7/389)and the jumping-out type of the MITE was predominant(8123%)in samples tested,suggesting high frequency transposition occurred in'Ca.L.asiaticus'strains recently collected from China.Biological roles of transposition of the active MITE remain to be determined.

关键词： Huanglongbing Candidatus Liberibacter asiaticus' miniature inverted-repeat transposable element MITE

Mapping the distributions of blood-sucking MITEs and MITE-borne agents in China:a modeling study

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Infectious Diseases of Poverty 2022年第2期11卷 99-99页

作者： Tao Wang Fanfei Meng Tianle Che Jinjin Chen Haiyang Zhang Yang Ji Zhengwei Fan Guoping Zhao Wenhui Zhang Baogui Jiang Qiang Xu Chenlong Lv Taoxing Shi Shiman Ruan Lanzheng Liu Wei Liu Yang Yang Liqun Fang State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology20 Dong-Da StreetFengtai DistrictBeijing 100071People’s Republic of China 不详 Department of Biostatistics College of Public Health and Health Professions and Emerging Pathogens InstituteUniversity of FloridaGainesvilleFLUSA

Background:Emerging MITE-borne pathogens and associated disease burdens in recent decades are raising serious public health concerns,yet their distributions and ecology remain under-investigated.We aim to describe the geographical distributions of blood-sucking MITEs and MITE-borne agents and to assess their ecological niches in China.Methods:We mapped 549 species of blood-sucking MITEs belonging to 100 genera at the county level and eight MITE-associated agents detected from 36 species of blood-sucking MITEs in China during 1978–2020.Impacts of climatic and environmental factors on the ecology of 21 predominant vector MITEs and a leading pathogen,Orientia tsutsugamushi,were assessed using boosted regression tree(BRT)models,and model-predicted risks were mapped.We also estimated the model-predicted number,area and population size of afected counties for each of the 21 MITE species in China.Results:Laelaps echidninus is the leading MITE species that potentially afects 744 million people,followed by La.jettmari(517 million)and Eulaelaps stabularis(452 million).Leptotrombidium scutellare is the MITE species harboring the highest variety of MITE-borne agents including four Rickettsia species and two viruses,followed by Eu.stabularis(2 agents),L.palpale(2)and La.echidninus(2).The top two agents that parasitize the largest number of MITE species are O.tsutsugamushi(28 species)and hantavirus(8).Mammalian richness,annual mean temperature and precipitation of the driest quarter jointly determine the ecology of the MITEs,forming four clusters of major MITE species with distinct geographic distributions.High-risk areas of O.tsutsugamushi are mainly distributed in southern and eastern coastal provinces where 71.5 million people live.Conclusions:Ecological niches of major MITE species and MITE-borne pathogens are much more extensive than what have been observed,necessitating expansion of current fled surveillance.

关键词： MITE MITE-borne pathogen MITE-borne disease Distribution Risk determinant China

Loss-of-Function of a Ubiquitin-Related Modifier Promotes the Mobilization of the Active MITE mPing

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Molecular Plant 2013年第3期6卷 790-801页

作者： Takuji Tsukiyama Shota Teramoto KanakoYasuda Akira Horibata Nanako Mori Yutaka Okumoto Masayoshi Teraishi Hiroki Saito Akiko Onishi Kanako Tamura Takatoshi Tanisaka Division of Agronomy and Horticulture Science Graduate School of Agriculture Kyoto University Kitashirakawa Sakyo Kyoto 606-8502 Japan Faculty of Biology-Oriented Science and Technology Kinki University Kinokawa Wakayama 649-6433 Japan

Miniature inverted-repeat transposable elements （MITEs） are widespread in both prokaryotic and eukaryotic genomes, where their copy numbers can attain several thousands. Little is known, however, about the genetic factor（s） affecting their transpositions. Here, we show that disruption of a gene encoding ubiquitin-like protein markedly enhances the transposition activity of a MITE roPing in intact rice plants without any exogenous stresses. We found that the transposition activity of roPing is far higher in the lines harboring a non-functional allele at the Rurml （Rice ubiquitin-related modifier-I） locus than in the wild-type line. Although the alteration of cytosine methylation pattern triggers the activation of transposable elements under exogenous stress conditions, the methylation degrees in the whole genome, the roPing-body region, and the roPing-flanking regions of the non-functional Rurml line were unchanged. This study provides experimental evidence for one of the models of genome shock theory that genetic accidents within cells enhance the transposition activities of transposable elements.

关键词： Oryza sativa MITE mPing ubiquitin-related modifier methylation genome shock theory.

山核桃MITE序列识别和MITE-PCR反应体系的建立

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分子植物育种 2020年第19期18卷 6424-6432页

作者：夏晓庆董昂曾燕如浙江农林大学省部共建亚热带森林培育国家重点实验室杭州311300

微型反向重复转座元件(miniature inverted repeat transposable elements,MITEs)在植物中广泛分布,可产生适应性的遗传变异,尤其可在非重组无性繁殖物种中产生变异,可用于遗传多样性分析。为促进山核桃的遗传学研究,本研究运用MITE Dig... 详细信息

微型反向重复转座元件(miniature inverted repeat transposable elements,MITEs)在植物中广泛分布,可产生适应性的遗传变异,尤其可在非重组无性繁殖物种中产生变异,可用于遗传多样性分析。为促进山核桃的遗传学研究,本研究运用MITE Digger软件对山核桃基因组测序数据进行扫描,共识别得到739条山核桃MITE序列。基于MITE序列,使用Primer-BLAST在线设计引物680对,占MITE序列的92.02%。基于小麦的MITE-PCR体系,通过降低退火温度5℃优化了山核桃的反应体系。随机挑选300对引物进行筛选,其中108对引物具有稳定的扩增产物。108对引物中100对引物在不同单株山核桃个体中扩增呈现单条带,占92.6%,8对引物扩增呈现双条带,占7.4%。PCR产物测序结果发现,呈现单条带的PCR扩增产物均包含MITE序列,但呈现双条带的PCR扩增产物中大多不含有MITE序列。研究结果为山核桃遗传多样性研究提供了科学依据。

关键词：山核桃(Carya cathayensis) 转座子 MITE

Quantitative impact of mating duration on reproduction and offspring sex ratio of Phytoseiulus persimilis(Acari: Phytoseiidae)

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Journal of Integrative Agriculture 2019年第4期18卷 884-892页

作者： Lü Jia-le ZHANG Bao-he JIANG Xiao-huan WANG En-dong XU Xue-nong Lab of Predatory MITEs Institute of Plant Protection Chinese Academy of Agricultural Sciences

Phytoseiulus persimilis is an important biological control agent, commercially produced worldwide. To increase understandings of its reproduction, herein we provided quantitative descriptions of the inflation and deflation of its spermathecae as a function of time and copulation duration, and the quantitative impact of copulation termination on volume of spermatheca vesicle, fecundity, and offspring sex ratio. After mating started, at least one spermatheca started to inflate immediately, but no egg was produced until spermatheca volume reached 1 021 μm3. Beyond this size, cumulative fecundity was linearly correlated with vesicle volume. Producing one egg required 36 μm3 volume increase of the vesicle. Each spermatheca vesicle reached its peak size at the end of mating duration(ca. 2.38 h after mating started), and started to shrink immediately. In 24 h, ca. 71% individuals had one completely shriveled spermatheca, while in 72 h all individuals had at least one shriveled spermatheca. The estimated maximum cumulative fecundity per female after a single mating is(69.4±7.7) eggs. No significant impact of mating termination on offspring sex ratio was observed after mating duration reached 60 min, while higher proportion of male offspring was observed when mating duration is 15 or 30 min. This study is the first step to investigate possible male impact on offspring sex ratio in P. persimilis. Our results suggested the number of sperms received during mating be a restriction factor of P. persimilis offspring sex ratio regulation.

关键词： predatory MITE pseudoarrhe no toky spermathecae fecun dity sex determi nation

A MITE insertion into the 3′-UTR regulates the transcription of TaHSP16.9 in common wheat

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The Crop Journal 2014年第6期2卷 381-387页

作者： Jingting Li Zhenzhong Wang Huiru Peng Zhiyong Liu College of Resources and Environment Science Pingdingshan University State Key Laboratory for Agrobiotechnology/Department of Plant Genetics & Breeding China Agricultural University

Miniature inverted-repeat transposable elements(MITEs) are a type of DNA transposon frequently inserted into promoters, untranslated regions(UTR), introns, or coding sequences of genes. We found a 276-bp tourist-like MITE insertion in the 3′-UTR of a 16.9 k Da small heat shock protein gene(TaH SP16.9-3A) on chromosome 3A of common wheat. Haplotype analysis revealed two haplotypes, s HSP-W(wild type without MITE insertion) and s HSP-M(mutant with MITE insertion), present in wheat germplasm. Both semiquantitative PCR and quantitative real-time PCR analyses showed increased transcription levels of TaH SP16.9-3A in s HSP-M compared with those of s HSP-W after heat treatment at 42 °C. It appeared that the MITE insertion into the 3′-UTR enhances the transcription of TaH SP16.9-3A.

关键词： MITE sHSP 3′-UTR Gene transcription Common wheat

Differential levels of MITE infestation of wheat and barley in Czech grain stores

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Insect Science 2009年第3期16卷 255-262页

作者： Jan Hubert Zuzana Kucerova Radek Aulicky Marta Nesvorna Vaclav Stejskal Crop Research Institute Prague Czech Republic

While MITEs are able to utilize numerous food sources, the suitability of the food strongly influences population growth. The different suitabilities of various stored agricultural products will thus affect the level of infestation. In this study, we compared field MITE infestation rates in two stored cereals： wheat and barley. We analyzed MITE abundance, frequency and species composition in samples of grain obtained from 79 selected Czech grain stores. Stored barley seemed to be more vulnerable to MITE attack than wheat, as we consistently found more infested samples, more species and higher mean and median MITE abundance per sample in barley as compared to wheat. The mean MITE abundance per sam- ple were 55 and 506 individuals for wheat and barley, respectively. In barley, 10% of sampies exceeded allergen risk threshold （i.e., 1 000 individuals per kg of grain）. Altogether, 25 species were identified from approximately 35 000 individuals. The most frequently identified species were the same in wheat and barley, that is, Tydeus interruptus Sig Thor, Acarus siro L., Tarsonemus granarius Lindquist, Lepidoglyphus destructor （Schrank） and Tyrophagus putrescentiae （Schrank）. Based on principal components analysis, we found a closer association of T. interruptus, T. putrescentiae, L. destructor and Cheyletus eruditus （Schrank） with barley samples, corresponding to the high frequency and abundance values of these MITEs. The probable reasons for the higher infestation, especially MITE abundance in barley, are discussed in relation to the higher proportion of crushed parts, which may release favorable nutrient sources and amplify the abundance values.

关键词： barley food grain MITE storage wheat

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小麦MITE扩增体系的建立

华北农学报 2014年第2期29卷 110-115页

作者：谢欢杨文香彭巧慧赵丽娟刘力伟张娜刘大群河北农业大学植物病理学系河北省农作物病虫害生物防治工程技术研究中心国家北方山区农业工程技术研究中心河北保定071001 河北省农林科学院河北石家庄050011 邯郸市种子管理站河北邯郸056002

以Thatcher及23个以Thatcher为遗传背景的小麦抗叶锈病近等基因系为材料,开展了MITE扩增体系的建立研究,获得了适合小麦MITE分子标记的扩增体系及不同引物的退火温度,所用56对引物有27对获得稳定扩增。

关键词：小麦叶锈病抗病基因 MITE 分子标记

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转座事件引发的重组可以在不同的时空发生

转座事件引发的重组可以在不同的时空发生

作者：吴红跃浙江大学

学位级别：硕士

二倍体水稻基因组上存在大量的转座元件序列,本研究利用生物信息学方法,将已获得的日本晴、93-11水稻的三组水稻全基因组序列进行比对,利用RESite方法在三组数据的差异性片段中筛选出具有微小倒置重复序列元件(miniature inverted-repea... 详细信息

二倍体水稻基因组上存在大量的转座元件序列,本研究利用生物信息学方法,将已获得的日本晴、93-11水稻的三组水稻全基因组序列进行比对,利用RESite方法在三组数据的差异性片段中筛选出具有微小倒置重复序列元件(miniature inverted-repeat transposable element,MITE)序列结构的 334 候选序列,选用水稻花药培养的手段应用转座子显示技术证明了单拷贝序列UC23、UC30、UC35单拷贝序列具有潜在的转座活性。又通过放宽筛选生物信息学的方法,重计算了存在于三组水稻全基因组序列中基因区内的差异MITE序列群,得到了一个大容量的候选MITE序列集合;在籼稻、粳稻和野生稻中比较了同源基因座位上同一转座元件的序列组成结构的多态性,获取了转座后发生重组事件的时空特征信息,证明了转座事件引发的重组可以在不同的时空发生。

关键词：转座元件重组 MITE LA-PCR,RESite方法

柑橘砧木枳和枳雀根系中FRO2基因的表达差异研究

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柑橘砧木枳和枳雀根系中FRO2基因的表达差异研究

作者：赵柳莹华中农业大学

学位级别：硕士

在我国重庆、四川、陕南、丹江水库等主要柑橘产区,是以石灰岩为母质的碱性土壤,柑橘果园缺铁黄化现象十分普遍,严重影响了柑橘的产量和品质。目前生产上常用的缺铁矫正措施不仅作用微小,而且容易对环境造成污染。众所周知,柑橘是以嫁... 详细信息

在我国重庆、四川、陕南、丹江水库等主要柑橘产区,是以石灰岩为母质的碱性土壤,柑橘果园缺铁黄化现象十分普遍,严重影响了柑橘的产量和品质。目前生产上常用的缺铁矫正措施不仅作用微小,而且容易对环境造成污染。众所周知,柑橘是以嫁接苗的形式进行繁育,通过选用不同的砧木和接穗进行组合扩繁,不同的砧木对养分的吸收和利用均有较大差异,因此通过筛选或培育耐缺铁的砧木是解决柑橘缺铁黄化问题公认的最经济的策略之一。本课题组前期在陕西城固的果园中发现了以枳雀为砧木的温州蜜柑具有良好的耐缺铁特性,并通过前期温室中的生理实验证实了枳雀的这一优良特性,但是其在分子水平的机制还不清楚。柑橘是双子叶植物,在铁吸收机制上属于机理I植物。机理I植物的根系主要通过铁的酸化、还原和转运三个过程共同完成铁的吸收。本研究以耐缺铁的柑橘砧木枳雀和对缺铁敏感的枳为材料,探究柑橘高铁还原酶FRO2基因的功能,比较两种砧木在缺铁下FRO2基因的表达以及FRO2启动子活性差异。通过对FRO2的研究,了解柑橘根系铁吸收过程的还原机制,为铁高效砧木资源的筛选和培育提供理论依据。本课题主要研究结果如下:1.枳和枳雀FRO2基因的开放阅读框均为2157 bp,编码718个氨基酸,但有19个氨基酸序列的差异。2.拟南芥突变体互补实验证明,柑橘的FRO2基因在缺铁条件下使拟南芥突变体fro2-1的表型部分恢复至野生型水平,维持拟南芥的铁稳态。3.枳和枳雀的实生苗在水培缺铁处理的条件下,FRO2基因的表达趋势都是先升高后降低,均在处理的第10 d达到峰值,但枳和枳雀FRO2基因表达量有所差异,在缺铁第10 d时,枳FRO2基因的表达量显著高于枳雀,是枳雀的1.86倍,缺铁第15 d时,枳雀FRO2基因的表达量显著高于枳,是枳的1.63倍。4.在缺铁处理第7 d时,Promoter::GUS实验结果显示枳的FRO2启动子的活性比枳雀强,但高铁还原酶显色实验结果为枳雀根系的高铁还原酶分泌比枳强。有可能存在基因转录后水平的调控导致枳雀和枳的高铁还原酶活性的差异。5.枳和枳雀FRO2启动子共预测到25个相同类型的功能元件,主要涉及光反应、非生物胁迫、植物激素应答和分生组织特异表达等,每个元件的数量存在部分差异。此外枳有2个特有的功能元件,分别为抗氧化反应相关元件ARE和光响应元件TCT-motif。枳雀有8个特有的功能元件,其中涉及乙烯反应的元件ERE和光响应相关的元件chs-CMA1a位于枳雀启动子MITE中。启动子顺式作用元件的差异可能与枳和枳雀在转录水平上的差异有关。

关键词：枳枳雀耐缺铁 FRO2 MITE 铁还原