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lipid analysis of three special nervonic acid resources in China

Oil Crop Science 2020年第4期5卷 180-186页

作者： Xinghao Tu Jingya Wan Ya Xie Fang Wei Siewyoung Quek Xin Lv Liqing Du Hong Chen Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences Key Laboratory of Oilseeds Processing of Ministry of Agriculture and Rural AffairsKey Laboratory of Biology and Genetic Improvement of Oil Crops of Ministry of Agriculture and Rural AffairsHubei Key Laboratory of Lipid Chemistry and NutritionWuhanChina South Subtropical Crop Research Institute Chinese Academy of Tropical Agricultural Science/Key Laboratory of Tropical Fruit BiologyMinistry of Agriculture and Rural AffairsZhanjiangChina Faculty of Life Science Hubei UniversityWuhanChina School of Chemical Sciences University of AucklandAuckland1142New Zealand

Nervonic acid plays an important role in nutrition and function of the human *** oleifera,Acer truncatum and Xanthoceras sorbifolium are China’s unique woody plant rich in nervonic acid in seed *** study aims to investigate the lipid composition of these 3 special *** fatty acids were detected by gas chromatography coupled with flame detector(GC-FID).Triglycerides(TAGs)and phospholipids(PLs)were detected by shotgun-mass spectrometry(shotgun-MS).Results showed that *** oil presented the highest level of nervonic acid(46.20±0.22%)among the 3 *** oil of *** and *** had 3.53±0.20%and 1.83±0.21%nervonic acid respectively.53 species of TAGs and 15 species of PLs were identified in *** oil,with PLs content of 499.94±22.34μg/*** *** oil,PL and TAG species were twice more than those in *** oil,and its’content of PLs was 76.27±3.21μg/*** *** oil,75 TAGs and 34 PLs were detected,with the lowest PLs at 23.84±0.17μg/*** results demonstrated that these 3 vegetable oils have great potential to become nervonic acid supplements for human health.

关键词： Nervonic acid Malania oleifera Acer truncatum Xanthoceras sorbifolium lipid analysis

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Isolation of Chloroplasts from Marine Microalga Isochrysis galbana Parke for Their lipid Composition analysis

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Journal of Ocean University of China 2022年第1期21卷 225-235页

作者： ZHONG Ye LI Yanrong XU Jilin CAO Jiayi ZHOU Chengxu YAN Xiaojun Key Laboratory of Applied Marine Biotechnology Ningbo UniversityNingbo 315211China Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture Ningbo UniversityNingbo 315211China Fujian Baozhi Aquatic Science and Technology Co. Ltd.Zhangzhou 363503China Ningbo Institute of Oceanography Ningbo 315211China

Marine microalga Isochrysis galbana is an important feed species with a high nutritional *** from other uni-cellular algae,its cell contains two chloroplasts which are the major sites for lipid ***,we optimized a chloroplast isola-tion approach suitable for the isolation of *** chloroplasts and determined the purity and integrity of the isolated chloroplasts through microscopic observations and enzyme activity *** chloroplast lipids were analyzed with a ultrahigh-performance li-quid chromatography-Q Exactive Orbitrap-mass *** newly developed isolation approach is simple and reliable to isolate chloroplasts with high integrity and *** average yield of intact chloroplasts was 15.3%±0.1%.Glycolipids and acyl-glycerols were the main chloroplast *** accounted for 56.6%of chloroplast ***(DGDG),monogalactosyldiacylglycerol(MGDG)and sulfoquinovosyldiacylglycerol(SQDG)were the main *** fatty acyl R1/R2 were mostly 18:4/16:1,18:3/16:1 and 18:4/18:5 in DGDGs,14:0/18:4,18:4/18:5,18:4/18:4 and 18:3/18:4 in MGDGs and 16:0/14:0,16:0/18:3,and 18:4/18:3 in *** addition,diacylglycerol(DAG)was the most abundant acylglycerols;the content of 22:6/18:4-DAG was the *** was a little amount of glycosphingolipid(GSL)in ***(DGMG),monogalactosylmonoglyceride(MGMG),sulfoquinovosylmonoacylglycerol(SQMG),monoglyceride(MAG),phospholi-pids(PLs),ceramide(Cer)and betaine lipids were nearly undetectable in *** fatty acid proportions of DGDGs,MGDGs,SQDGs,DAGs,triglycerides(TAGs)and GSLs were either higher or lower than or similar to those of ***,our isolation approach is applicable to many aspects of chloroplast biology,and may offer a reference for the isolation of chloroplasts from other marine microalgae.

关键词： Isochrysis galbana chloroplast isolation method lipid analysis chloroplast lipid

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Linking microbial community analysis and ecosystem studies: A rapid lipid analysis protocol for high throughput

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Soil Ecology Letters 2019年第1期1卷 22-32页

作者： Teri C.Balser Chao Liang Jessica L.M.Gutknecht Department of Soil Science University of Wisconsin-Madison1525 Observatory DriveMadisonWI 53706USA Faculty of Science and Engineering Curtin UniversityPerthWestern Australia6845 Australia Institute of Applied Ecology Chinese Academy of SciencesShenyang 110016China Department of Soil Waterand ClimateUniversity of Minnesota439 Borlaug Hall1991 Upper Buford CircleSt.PaulMN 55108USA

During the past two decades interest in linking soil microbial community composition and activity with ecosystem scale field studies of nutrient cycling or plant community response to disturbances has *** its importance there are challenges in making this *** is the question of analytical *** general,microbiological community-level methodologies have not been readily adaptable to the large sample sizes necessary for ecosystem-scale *** a result,it has been difficult to generate compatible microbial and ecosystem data *** lipid analysis shows potential as a middle ground between simple biomass measures and molecular ***,the two protocols that have most often been followed are either rapid but indiscriminate(total lipid analysis or fatty acid methyl ester analysis;FAME),or precise but time consuming(phospholipid fatty acid analysis;PLFA).In this paper we report results from a standardized soil used test a modified extraction method(the‘hybrid’method)developed to balance the speed of FAME and the precision of PLFA in order to increase sample *** comparing the three methods,we find that FAME and PLFA are qualitatively and quantitatively *** FAME method yielded the highest fatty acid abundance,but also had high variance resulting in low *** PLFA method had precision,but low ***‘hybrid’method fell midway between FAME and PLFA for quantitative fatty acid *** addition,the hybrid extraction can be completed in a fraction of the time it takes for *** hybrid protocol appears to provide an optimal balance between effort and accuracy and therefore is a good choice for large-scale ecosystem studies.

关键词： Microbial community composition lipid analysis PLFA MIDI-FAME Ecosystem studies

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Ozone-induced dissociation implemented with a dual-trap mass spectrometer for lipid analysis

Ozone-induced dissociation implemented with a dual-trap mass...

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2018年中国质谱学术大会（CMSC2018）

作者： Xinwei Liu Xiaoxiao Ma Stephen J.Blanksby Xia Yu Zheng Ouyang State Key Laboratory of Precision Measurement Technology and Instruments Department of Precision InstrumentTsinghua Universityand Department of Chemistry Central Analytical Research Facility Queensland University of Technology

With discontinuous atmospheric pressure interface(DAPI), lipids in biological samples can now be readily sampled, ionized, and analyzed using miniature systems with ambient ionization methods. Recently, dual-trap analyzer system was developed for Mini β instrument to enable versatile scan functions, including beam-type CID that was a unique addition to a trap instrument. Real time pressure control with gas leaking was implemented to facilitate the ion transfer as well as the coupling of gas-phase reactions for lipid analysis. Ozone-induced dissociation(OzID) was implemented with high efficiency for lipid structure analysis or confirmation. A modified Mini β instrument was used for this experiment, with a DAPI for ion introduction, two linear ion traps(LITs) installed for MS analysis and a nanoESI for ionization. lipid ions were trapped in LIT I, and sequentially, mass-selectively transferred to LIT II for MS/MS analysis. An ozone generator was built using dielectric barrier discharge system. A pulse valve was used to introduce ozone into LIT II for reaction with lipid ions trapped. The OzID efficiency was observed to be high when the manifold pressure was higher than 5 m Torr. Phosphatidylcholines(PCs) and fatty acids(FAs) were used to test the performance of the OzID effectiveness. As a demonstration, sodiated FA 18:2(10 E, 12 Z)(m/z 325) was mass-selectively transferred from LIT I to LIT II with minimum kinetic energy, while the pulse valve connected to the ozone generator was opened for 10 ms. Fragment ions characteristic to OzID at m/z 231, 257, and 271,· due to cleavage at each C=C location, were clearly observed and the OzID efficiency was estimated to be higher than 50%. Ozone reaction with sodiated PC(16:0/18:1(9 Z))(m/z 782) also produced characteristic OzID fragments at m/z 672 and 688, with about an OzID efficiency of 10% for 100 ms ozone injection. OzID was utilized to resolve the structure of a unique fragment ion(m/z 419) discovered from CID of bicarbo

关键词： OzID lipid analysis lipid C=C location isomers miniature mass spectrometer ion trap

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LC-SWATH/MS Metabolomics Platform with Hyphenation of Extraction and analysis of Polar and Non-polar Metabolites in Biological Samples

LC-SWATH/MS Metabolomics Platform with Hyphenation of Extrac...

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2018年中国质谱学术大会（CMSC2018）

作者： Michel Raetz Renzo Picenon Guenter Boehm Gérard Hopfgartner Department of Inorganic and Analytical Chemistry Life Sciences Mass SpectrometryUniversity of Geneva

Sample preparation workflows for metabolomics studies of various sample types are often done on the basis of liquid-liquid extraction. Most commonly Bligh and Dyer extraction or one of its variants. This step is generally performed to separate polar endogenous metabolites from non-polar compounds such as lipids. We propose an integrated workflow using a UHPLC-SWATH/MS platform for the metabolomics analysis of plasma samples combined with an automated Bligh and Dyer extraction on a robotic sample preparation system. lipids are separated into three fractions using a mixed mode μSPE. The resulting three fractions are analyzed on a hyphenated HILICSWATH/MS system. The solid phase extraction offers an attractive alternative to equalize the abundance of the different lipid classes in a sample. As a consequence injection volumes and therefore dynamic ranges can be optimized without saturating the system with higher abundant lipid classes or species.

关键词： sample preparation lipid analysis metabolomics

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Extraction of Fat and Fatty Acid Composition from Slaughterhouse Waste by Evaluating Conventional Analytical Methods

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American Journal of Analytical Chemistry 2021年第5期12卷 202-225页

作者： Ariba Khan Farah Naz Talpur Muhammad Iqbal Bhanger Syed Ghulam Musharraf Hassan Imran Afridi National Centre of Excellence in Analytical Chemistry University of Sindh Jamshoro Pakistan Hussain Ebrahim Jamal Research Institute of Chemistry International Centre for Chemical and Biological Sciences University of Karachi Karachi Pakistan

To attain maximum recovery of useful compounds from slaughterhouse waste (Suet, Tongue, Pancreas) of selected ruminant (cow, goat, lamb, and bull), the fat extraction efficiency of popular methods was compared along with fatty acid (FAs) composition. Four selected methods including Soxhlet (SOX), acid hydrolysis, Bligh & dyer (B&D), and Folch (FOL) were assessed. After methylation, extracted lipids were analyzed by Gas chromatography for FA composition. Data indicated that all selected methods were significantly (p lipid-containing products (Tongue, Pancreas) as well as their respective FA Composition. Based on analysis of Variance and Principal component analysis, the effective method for lipid and FA Composition analysis was the FOL method. The Soxhlet method was only effective for samples with high-fat content i.e., suet, while the B&D method gave comparatively low lipid content in analyzed samples. Hence based on the results, excellent fat and fatty acid extraction was achieved with the FOL method.

关键词： Fatty Acid Slaughterhouse Waste Animal by-Products Extraction Methods lipid analysis Gas Chromatography

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