LC-SWATH/MS Metabolomics Platform with Hyphenation of Extraction and Analysis of Polar and Non-polar Metabolites in Biological Samples
作者单位：Department of Inorganic and Analytical ChemistryLife Sciences Mass SpectrometryUniversity of Geneva
会议名称：《2018年中国质谱学术大会（CMSC2018）》
会议日期：2018年
学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学]
关 键 词：sample preparation lipid analysis metabolomics
摘 要：Sample preparation workflows for metabolomics studies of various sample types are often done on the basis of liquid-liquid extraction. Most commonly Bligh and Dyer extraction or one of its variants. This step is generally performed to separate polar endogenous metabolites from non-polar compounds such as lipids. We propose an integrated workflow using a UHPLC-SWATH/MS platform for the metabolomics analysis of plasma samples combined with an automated Bligh and Dyer extraction on a robotic sample preparation system. Lipids are separated into three fractions using a mixed mode μSPE. The resulting three fractions are analyzed on a hyphenated HILICSWATH/MS system. The solid phase extraction offers an attractive alternative to equalize the abundance of the different lipid classes in a sample. As a consequence injection volumes and therefore dynamic ranges can be optimized without saturating the system with higher abundant lipid classes or species.