Isolation of Chloroplasts from Marine Microalga Isochrysis galbana Parke for Their Lipid Composition Analysis

作     者：ZHONG Ye LI Yanrong XU Jilin CAO Jiayi ZHOU Chengxu YAN Xiaojun ZHONG Ye;LI Yanrong;XU Jilin;CAO Jiayi;ZHOU Chengxu;YAN Xiaojun

作者机构：Key Laboratory of Applied Marine BiotechnologyNingbo UniversityNingbo 315211China Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy AquacultureNingbo UniversityNingbo 315211China Fujian Baozhi Aquatic Science and Technology Co.Ltd.Zhangzhou 363503China Ningbo Institute of OceanographyNingbo 315211China 

出 版 物：《Journal of Ocean University of China》 (中国海洋大学学报（英文版）)

年 卷 期：2022年第21卷第1期

页      面：225-235页

核心收录：

学科分类：090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 09[农学] 

基　　金：supported by the Ningbo Science and Technology Research Projects(No.2019B10006) the Na-tional Key Research and Development Program of China(No.2019YFD0900400) the Zhejiang Major Science Pro-ject(No.2019C02057) the China Agriculture Research System of MOF and MARA,the Natural Science Founda-tion of Ningbo(No.2019A610416) the Ningbo Science and Technology Research Projects(No.2019C10023) the National Natural Science Foundation of China(No.31801724) 

主　　题：Isochrysis galbana chloroplast isolation method lipid analysis chloroplast lipid 

摘      要：Marine microalga Isochrysis galbana is an important feed species with a high nutritional *** from other uni-cellular algae,its cell contains two chloroplasts which are the major sites for lipid ***,we optimized a chloroplast isola-tion approach suitable for the isolation of *** chloroplasts and determined the purity and integrity of the isolated chloroplasts through microscopic observations and enzyme activity *** chloroplast lipids were analyzed with a ultrahigh-performance li-quid chromatography-Q Exactive Orbitrap-mass *** newly developed isolation approach is simple and reliable to isolate chloroplasts with high integrity and *** average yield of intact chloroplasts was 15.3%±0.1%.Glycolipids and acyl-glycerols were the main chloroplast *** accounted for 56.6%of chloroplast ***(DGDG),monogalactosyldiacylglycerol(MGDG)and sulfoquinovosyldiacylglycerol(SQDG)were the main *** fatty acyl R1/R2 were mostly 18:4/16:1,18:3/16:1 and 18:4/18:5 in DGDGs,14:0/18:4,18:4/18:5,18:4/18:4 and 18:3/18:4 in MGDGs and 16:0/14:0,16:0/18:3,and 18:4/18:3 in *** addition,diacylglycerol(DAG)was the most abundant acylglycerols;the content of 22:6/18:4-DAG was the *** was a little amount of glycosphingolipid(GSL)in ***(DGMG),monogalactosylmonoglyceride(MGMG),sulfoquinovosylmonoacylglycerol(SQMG),monoglyceride(MAG),phospholi-pids(PLs),ceramide(Cer)and betaine lipids were nearly undetectable in *** fatty acid proportions of DGDGs,MGDGs,SQDGs,DAGs,triglycerides(TAGs)and GSLs were either higher or lower than or similar to those of ***,our isolation approach is applicable to many aspects of chloroplast biology,and may offer a reference for the isolation of chloroplasts from other marine microalgae.