检索结果-南通市图书馆

Neural Regeneration Research 2011年第19期6卷 1452-1456页

作者： Yufeng Lin Yadan Li Dawei Zang Graduate School of Tianjin Medical University Tianjin 300070 China Department of Neurology Tianjin First Center Hospital Tianjin 300192 China

leukemia inhibitory factor （LIF） has been shown to promote proliferation of endogenous neural stem cells. In this study, we treated mice with cerebral infarction using LIF to investigate whether the LIF receptor subunit glycoprotein （gp）130 is involved in neuroprotection. After LIF treatment, the motor function of model mice was significantly improved. Immunofluorescence histochemistry showed increased numbers of endogenous neural stem cells surrounding the infarct foci. Western blot analysis revealed that gp130 expression was significantly decreased surrounding the infarcted foci. Results demonstrated that LIF promoted the proliferation of endogenous neural stem cells by inhibiting gp130 protein expression.

关键词： leukemia inhibitory factor endogenous neural stem cell glycoprotein-130 cerebral infarction proliferation neural regeneration

Effect of leukemia inhibitory factor on embryonic stem cell differentiation:implications for supporting neuronal differentiation

在线全文

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2006年第1期27卷 80-90页

作者： Zhao HE~(2,3) Jing-jing LI~(2,3) Chang-hong ZHEN~(3,4) Lin-ying FENG~3 Xiao-yan DING~(2,5)2 Laboratory of Molecular and Cell Biology and Laboratory of Stem Cell Biology,Institute of Biochemistry and Cell Biology:Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,China 3 Shanghai Institute of Materia Medica.Chinese Academy of Sciences.Shanghai 201203,China 4 Graduate School of the Chinese Academy of Sciences,Shanghai 200031,China. Laboratory of Molecular and Cell Biology and Laboratory of Stem Cell Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China Shanghai Institute of Materia Medica Chinese Academy of Sciences Shanghai China Graduate School of the Chinese Academy of Sciences Shanghai China

Aim:leukemia inhibitory factor (LIF),a pleiotropic cytokine,has been used ex-tensively in the maintenance of mouse embryonic stem cell *** thiscurrent work,we examined the effect of the LIF signaling pathway in embryonicstem (ES) cell differentiation to a neural ***:In the presence of LIF(1000U/mL),the production of neuronal cells derived from embryoid bodies (EB)was tested under various culture *** of the LIF pathway wasexamined with specific *** effects of cell apoptosis and proliferationon neural differentiation were *** cell differentiation into three-germlayers was ***:Under various culture conditions,neuronal differ-entiation was increased in the presence of *** the LIF-activated STAT3signaling pathway with specific inhibitors abolished the neuronal differentiationof ES cells,whereas inhibition of the LIF-activated MEK signaling pathway im-paired the differentiation of ES cells toward a glial *** suppressed cellapoptosis and promoted cell proliferation during ES cell *** inhib-ited the differentiation of ES cells to both mesoderm and extraembryonic endo-derm fates,but enhanced the determination of neural ***:These results suggest that LIF plays a positive role during the differentiation ofES cells into neuronal cells.

关键词： leukemia inhibitory factor embryonic stem cells neural fate embryoid bodies

Effects of leukemia inhibitory factor and basic fibroblast growth factor on free radicals and endogenous stem cell proliferation in a mouse model of cerebral infarction

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2012年第19期7卷 1469-1474页

作者： Weihui Huang Yadan Li Yufeng Lin Xue Ye Dawei Zang Department of Neurology Tianjin First Center HospitalTianjin Medical UniversityTianjin 300192China

The present study established a mouse model of cerebral infarction by middle cerebral artery occlusion, and monitored the effect of 25 tJg/kg leukemia inhibitory factor and （or） basic fibroblast growth factor administration 2 hours after model establishment. Results showed that following administration, the number of endogenous neural stem cells in the infarct area significantly increased, malondialdehyde content in brain tissue homogenates significantly decreased, nitric oxide content, glutathione peroxidase and superoxide dismutase activity significantly elevated, and mouse motor function significantly improved as confirmed by the rotarod and bar grab tests. In particular, the effect of leukemia inhibitory factor in combination with basic fibroblast growth factor was the most significant. Results indicate that leukemia inhibitory factor and basic fibroblast growth factor can improve the microenvironment after cerebral infarction by altering free radical levels, improving the quantity of endogenous neural stem cells, and promoting neurological function of mice with cerebral infarction.

关键词： leukemia inhibitory factor basic fibroblast growth factor endogenous neural stem cells free radical malondialdehyde nitric oxide glutathione peroxidase superoxide dismutase neuroprotection

leukemia inhibitory factor Enhanced the Developmental and Implantation Compatibility of Mouse Embryos in Co-culture with Human Endometrial Epithelial Cells

在线全文

学校读者我要写书评

暂无评论

Reproductive and Developmental Medicine 2021年第4期5卷 199-205页

作者： Ali Hosseini Bahar Movaghar Showra Amani Abkenari Hassan Nazari Mehrdad Bakhtiyari Department of Anatomy Faculty of MedicineIran University of Medical SciencesTehranIran Cellular and Molecular Research Center Iran University of Medical SciencesTehranIran Department of Embryology Reproductive Biomedicine Research CenterRoyan Institute for Reproductive BiomedicineACECRTehranIran Department of Anatomy School of MedicineTehran University of Medical SciencesTehranIran Research Institute of Animal Embryo Technology Shahrekord UniversityShahrekordIran

Objective:Among the variousin vitro embryo culture systems,co-culture has demonstrated remarkable effects in pre-implantation embryo development owing to the production of embryo-nourishing ***,little is known about the secretion of these ***,in this study,the effect of leukemia inhibitory factor(LIF),one of the most important nourishing factors in the early development of mouse embryos,in human endometrial epithelial cells(hEECs)was ***:Two-cell stage embryos were collected from the oviducts of hyper-stimulated and mated mice and cultivated in a co-culture with an hEEC monolayer with or without *** quality and developmental and attachment potential rates of cultured embryos were evaluated by determining the levels of octamer-binding transcription factor 4(Oct4)and caudal type homeobox 2(Cdx2)***:LIF significantly increased the developmental rate(82.67%vs.61.04%,respectively)and attachment rate(64%vs.45.45%,respectively)of mouse embryos co-cultured with hEECs compared to those in untreated *** expression levels ofOct4 andCdx2 in blastocysts cultured in the presence of LIF were higher than those in blastocysts cultured without ***:Despite the secretion of LIF by hEECs during co-culture with embryos,the amount of this factor was insufficient,and its addition to the culture media could increase the developmental potential of embryos.

关键词： Caudal Type Homeobox 2 Embryo Development leukemia inhibitory factor Octamer-Binding Transcription factor 4

Expression of leukemia inhibitory factor in Airway Epithelial Tissue of Asthmatic Rats

在线全文

学校读者我要写书评

暂无评论

Journal of Huazhong University of Science and Technology(Medical Sciences) 2007年第4期27卷 372-374页

作者：熊维宁曾大雄徐永健熊盛道方慧娟曹勇宋青凤曹超 Department of Respiratory Diseases Tongji HospitalTongji Medical CollegeHuazhong University of Science and Technology

In order to investigate the expression of leukemia inhibitory factor （LIF） in airway epithelial tissues of normal and asthmatic rats, the influence of dexamethasone and the role of LIF in pathogenesis of asthma, 30 Sprague-Dawley （SD） rats were randomly divided into 3 groups （10 for each group）： normal group, asthma model group, and dexamethasone-interfered group. In asthma model group and dexamethasone-interfered group, asthma rat models were established by intraperitoneal （i.p.） injection of 10% ovalbumin （OVA） and challenge with 1% OVA via inhalation. Rats in dexamethasone-interfered group were pretreated with dexamethasone （2 mg/kg, i.p） 30 min before each challenge. The expression of LIF protein in lung was detected by immunohistochemistry. The results showed that LIF protein was mainly expressed in cytoplasm of bronchial epithelial cells. The expression of LIF protein in the airway epithelial tissue of asthma model group was significantly higher than that in normal group and dexamethasone-interfered group （P〈0.01）, but there was no significant difference between normal group and dexamethasone-interfered group （P〉0.05）. It was concluded that the expression of LIF was increased significantly in the airway epithelial tissue of the asthma rats, and dexamethasone could down-regulate the expression of LIF. It was suggested that LIF might play an important role in the pathogenesis of asthma as an inflammation regulator.

关键词： bronchial asthma leukemia inhibitory factor immunohistochemistry

Glycemic control predicts the risk of hepatic fibrosis in biopsy-proven NAFLD:a possible mediating role for leukemia inhibitory factor?

在线全文

学校读者我要写书评

暂无评论

iLIVER 2022年第1期1卷 30-34页

作者： Feng Gong Kenneth I.Zheng Liang-Jie Tang Gang Li Rafael S.Rios Ou-Yang Huang Yang-Yang Li Christopher D.Byrne Giovanni Targher Man Mi Na He Hong-Lei Ma Ming-Hua Zheng Xi'an Medical University China NAFLD Research Center Department of HepatologyThe First Affiliated Hospital of Wenzhou Medical UniversityWenzhouChina Department of Pathology The First Affiliated Hospital of Wenzhou Medical UniversityWenzhouChina Southampton National Institute for Health Research Biomedical Research Centre University Hospital SouthamptonSouthampton General HospitalSouthamptonUK Section of Endocrinology Diabetes and MetabolismDepartment of MedicineUniversity and Azienda Ospedaliera Universitaria Integrata of VeronaVeronaItaly The First Affiliated Hospital of Xi'an Medical University Xi'anChina Institute of Hepatology Wenzhou Medical UniversityWenzhouChina Key Laboratory of Diagnosis and Treatment for the Development of Chronic Liver Disease in Zhejiang Province WenzhouChina

Background:Despite the clear link between nonalcoholic fatty liver disease(NAFLD)and type 2 diabetes mellitus,little is understood about how glycemic control impacts histological *** aimed to investigate the deeper association between hemoglobin A1c(HbA1c)and the histologic severity of liver ***:A total of 568 adults with biopsy-proven NAFLD from the PERSONS cohort in Wenzhou were *** association between mean HbA1c and hepatic histological features was investigated with ordinal logistic *** additive models were used to identify the non-linear relationship between HbA1c and increased fibrosis stage(stage F3).Causal mediation analysis was performed to calculate the indirect effect of the relationship between HbA1c and hepatic fibrosis mediated by ***:Every 1%increase in mean HbA1c was associated with 16%higher odds of increased fibrosis stage(odds ratio 1.16,95%CI 1.04–1.30),even after adjustment for confounding *** was a non-linear association between HbA1c and increased fibrosis stage(stage F3),and the HbA1c inflection point was 9.2%.In particular,the ORs on the left and right sides of this inflection point were 2.1(95%CI 1.4–3.3)and 0.1(95%CI 0–4.8),respectively.7%of the association(OR 1.07,95%CI 1.01–1.12)between HbA1c and liver fibrosis was mediated by leukemia inhibitory ***:Glycemic control predicts severity of hepatic fibrosis and leukemia inhibitory factor plays an intermediary role between them,and thus optimizing glycemic control may be a means of modifying the risk of fibrosis progression in NAFLD.

关键词： NAFLD leukemia inhibitory factor Hepatic fibrosis

BMPRⅡ^(+)neural precursor cells isolated and characterized from organotypic neurospheres:an in vitro model of human fetal spinal cord development

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2024年第2期19卷 447-457页

作者： Michael W.Weible II Michael D.Lovelace Hamish D.Mundell Tsz Wai Rosita Pang Tailoi Chan-Ling Bosch Institute Discipline of Anatomy and Histology(F13)University of SydneySydneyNSWAustralia School of Environment and Science Griffith UniversityNathanQLDAustralia Discipline of Medicine Nepean Clinical SchoolFaculty of Medicine and HealthUniversity of SydneyKingswoodNSWAustralia New South Wales Brain Tissue Resource Centre School of Medical SciencesFaculty of Medicine and HealthUniversity of SydneyCharles Perkins Centre(D17)SydneyNSWAustralia

Roof plate secretion of bone morphogenetic proteins(BMPs)directs the cellular fate of sensory neurons during spinal cord development,including the formation of the ascending sensory columns,though their biology is not well ***-ⅡBMP receptor(BMPRⅡ),the cognate receptor,is expressed by neural precursor cells during embryogenesis;however,an in vitro method of enriching BMPRⅡ^(+)human neural precursor cells(hNPCs)from the fetal spinal cord is *** was undertaken on intact second-trimester human fetal spinal cord using antibodies to BMPRⅡand leukemia inhibitory factor(LIF).Regions of highest BMPRⅡ^(+)immunofluorescence localized to sensory *** and meningeal-associated BMPRⅡ^(+)vascular cells were identified in both intact fetal spinal cord and cortex by co-positivity with vascular lineage markers,CD34/*** immunostaining identified a population of somas concentrated in dorsal and ventral horn interneurons,mirroring the expression of LIF receptor/CD118.A combination of LIF supplementation and high-density culture maintained culture growth beyond 10 passages,while synergistically increasing the proportion of neurospheres with a stratified,*** neurospheres were characterized by BMPRⅡ^(+)/MAP2ab^(+/–)/βⅢ-tubulin^(+)/nestin^(–)/vimentin^(–)/GFAP^(–)/NeuN^(–)surface hNPCs surrounding a heterogeneous core ofβⅢ-tubulin^(+)/nestin^(+)/vimentin^(+)/GFAP^(+)/MAP2ab^(–)/NeuN^(–)multipotent *** cultures from tripotential neurospheres contained neuronal(βⅢ-tubulin^(+)),astrocytic(GFAP+),and oligodendrocytic(O4+)lineage ***-activated cell sorting-sorted BMPRⅡ^(+)hNPCs were MAP2ab^(+/–)/βⅢ-tubulin^(+)/GFAP^(–)/O4^(–)in *** is the first isolation of BMPRⅡ^(+)hNPCs identified and characterized in human fetal spinal *** data show that LIF combines synergistically with high-density reaggregate cultures to support the organotypic reorganization of neurospheres,characterize

关键词： BMPRⅡ bone morphogenetic protein histotypic human spinal cord development leukemia inhibitory factor neurosphere organotypic reaggregate sensory columns

The neuroprotective role of Wnt signaling in the retina

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2021年第8期16卷 1524-1528页

作者： Stefan Kassumeh Gregor R.Weber Matthias Nobl Siegfried G.Priglinger Andreas Ohlmann Department of Ophthalmology University HospitalLMU MunichMathildenstrasse 880336 MunichGermany

The canonical Wnt/β-catenin signaling pathway has been shown to play a major role during embryonic development and maturation of the central nervous system including the retina. It has a significant impact on retinal vessel formation and maturation, as well as on the establishment of synaptic structures and neuronal function in the central nervous system. Mutations in components of the Wnt/β-catenin signaling cascade may lead to severe retinal diseases, while dysregulation of Wnt signaling can contribute to disease progression. Apart from the angiogenic role of Wnt/β-catenin signaling, research in the last decades leads to the theory of a protective effect of Wnt/β-catenin signaling on damaged neurons. In this review, we focus on the neuroprotective properties of the Wnt/β-catenin pathway as well as its downstream signaling in the retina.

关键词： apoptosis β-catenin leukemia inhibitory factor Müller cells neurodegeneration neuroprotection Norrin photoreceptors retina retinal ganglion cells Wnt

Optical coherence tomography and T cell gene expression analysis in patients with benign multiple sclerosis

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2017年第8期12卷 1352-1356页

作者： John Soltys Qin Wang Yang Mao-Draayer University of Colorado Medical Scientist Training Program(MSTP) AuroraCOUSA Department of Neurology University of Michigan Medical SchoolAnn ArborMIUSA

Benign multiple sclerosis is a retrospective diagnosis based primarily on a lack of motor symptom progression. Recent findings that suggest patients with benign multiple sclerosis experience non-motor symptoms highlight the need for a more prospective means to diagnose benign multiple sclerosis early in order to help direct patient care. In this study, we present optical coherence tomography and T cell neurotrophin gene analysis findings in a small number of patients with benign multiple sclerosis. Our results demonstrated that retinal nerve fiber layer was mildly thinned, and T cells had a distinct gene expression profile that included upregulation of interleukin 10 and leukemia inhibitory factor, downregulation of interleukin 6 and neurotensin high affinity receptor 1（a novel neurotrophin receptor）. These findings add evidence for further investigation into optical coherence tomography and m RNA profiling in larger cohorts as a potential means to diagnose benign multiple sclerosis in a more prospective manner.

关键词： neurotensin high affinity receptor 1 benign multiple sclerosis optical coherence tomography interleukin 10 T cell leukemia inhibitory factor optic neuritis neural regeneration

Synchronous gastric cancer complicated with chronic myeloid leukemia (multiple primary cancers):A case report

在线全文

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2022年第30期10卷 11146-11154页

作者： Yong-Xun Zhao Ze Yang Li-Bin Ma Jia-Yao Dang Hui-Ying Wang The Seventh Department of General Surgery The First Hospital of Lanzhou UniversityLanzhou 730000Gansu ProvinceChina The First Clinical Medical School The First Clinical Medical School of Lanzhou UniversityLanzhou 730000Gansu ProvinceChina

BACKGROUND With the advancement of medical technology and improvement in living standards,the incidence of multiple primary cancers has gradually *** particular,tumors of the digestive system account for a large proportion of multiple primary *** diagnosis and treatment of chronic myeloid leukemia,particularly with synchronous gastric cancer,at the first consultation is relatively *** SUMMARY Herein,we present the case of a middle-aged man who was referred to the Department of Hematology owing to an elevated white blood cell *** the examination,he was diagnosed with chronic myeloid leukemia and was administered *** months after the initial diagnosis,he visited our hospital again for abdominal pain,and further examination revealed gastric *** discussion with a multidisciplinary team,S-1(Tegafur,Gimeracil,and Oteracil Potassium Capsules) combined with oxaliplatin—SOX regimen—was ***,the patient’s condition rapidly *** developed colonic obstruction and underwent an ostomy;however,he died less than 6 months after the initial *** Multiple primary cancers are influenced by environmental and genetic factors;a standardized multidisciplinary discussion plays a key role in treatment.

关键词： Multiple primary cancers Gastric cancer leukemia leukemia inhibitory factor Case report