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Effect of leukemia inhibitory factor on embryonic stem cell differentiation:implications for supporting neuronal differentiation

Effect of leukemia inhibitory factor on embryonic stem cell differentiation:implications for supporting neuronal differentiation

作     者:Zhao HE~(2,3) Jing-jing LI~(2,3) Chang-hong ZHEN~(3,4) Lin-ying FENG~3 Xiao-yan DING~(2,5)2 Laboratory of Molecular and Cell Biology and Laboratory of Stem Cell Biology,Institute of Biochemistry and Cell Biology:Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,China 3 Shanghai Institute of Materia Medica.Chinese Academy of Sciences.Shanghai 201203,China 4 Graduate School of the Chinese Academy of Sciences,Shanghai 200031,China. 

作者机构:LaboratoryofMolecularandCellBiologyandLaboratoryofStemCellBiologyInstituteofBiochemistryandCellBiology ShanghaiInstitutesforBiologicalSciencesChineseAcademyofSciencesShanghai200031China 

出 版 物:《Acta Pharmacologica Sinica》 (中国药理学报(英文版))

年 卷 期:2006年第27卷第1期

页      面:80-90页

核心收录:

学科分类:1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学] 

基  金:Project supported by the National Key Project for Basic Science Research of China(2001CB509901) the Shanghai Key Project for Basic Science Research(03DJ14019) 

主  题:leukemia inhibitory factor embryonic stem cells neural fate embryoid bodies 

摘      要:Aim:Leukemia inhibitory factor (LIF),a pleiotropic cytokine,has been used ex-tensively in the maintenance of mouse embryonic stem cell *** thiscurrent work,we examined the effect of the LIF signaling pathway in embryonicstem (ES) cell differentiation to a neural ***:In the presence of LIF(1000U/mL),the production of neuronal cells derived from embryoid bodies (EB)was tested under various culture *** of the LIF pathway wasexamined with specific *** effects of cell apoptosis and proliferationon neural differentiation were *** cell differentiation into three-germlayers was ***:Under various culture conditions,neuronal differ-entiation was increased in the presence of *** the LIF-activated STAT3signaling pathway with specific inhibitors abolished the neuronal differentiationof ES cells,whereas inhibition of the LIF-activated MEK signaling pathway im-paired the differentiation of ES cells toward a glial *** suppressed cellapoptosis and promoted cell proliferation during ES cell *** inhib-ited the differentiation of ES cells to both mesoderm and extraembryonic endo-derm fates,but enhanced the determination of neural ***:These results suggest that LIF plays a positive role during the differentiation ofES cells into neuronal cells.

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