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Genetic Analysis of Five Porphyra Species by isozyme Markers

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Marine Science Bulletin 2010年第1期12卷 65-78页

作者：沈颂东李艳燕许璞张美如袁昭兰王建伟朱建一苏州大学生命科学院江苏苏州215123 常熟理工食品工程与生物系江苏苏州215500 江苏省海产渔业研究所江苏南通226007

Vertical polyamide gel electrophoresis was used to investigate isozyme polymorphisms among different isolates （including wild and cultivated） of Porphyra katadai, Porphyra oligospermatangia, Porphyra yezoensis, Porphyra haitanensis, and a hybridize species （Porphyra yezoensis x Porphyra haitanensis） sampled from China. Whereafter, the analyses of probable minimum loci numbers, observed alleles sum, genetic diversity, and unweighted pair-group arithmetic average （UPGMA） cluster were carded out. After initial activity and resolution testing of bands of 23 enzymes, 6 of them （MDH, ME, LDH, GDH, IDH and G-6-PDH） were proved to be appropriate for analysis of the full sample set. The probable minimum numbers of loci and alleles analyses showed that the five species of Porphyra had an extraordinary consistent result in ME loci and alleles. However, P. katadai and P. oligospermatangia differed from other three species of Porphyra in LDH and GDH loci and alleles. P. katadai was independent in the analyses of MDH and P. oligosperTnatangia and P. haitanensis differed from other three species in IDH analyses. Moreover, P. yezoensis and P. haitanensis were apart from other three species in G-6-PDH analysis. Taking one with another, P. katadai was relatively separated in the probable minimum numbers of loci and alleles analyses. The results indicated that the genetic variation among the five Porphyra species was limited with a genetic identity of 0.7550. The hybridize species （P. yezoensis x P. haitanensis） seemed to be high homologue with P. oligospermatangia, unexpectedly got relatively lower average genetic identities with both P. yezoensis and P. haitanensis. The 4 strains of P. yezoensis were relatively divergent with an average genetic identity of 0.7428, and P. katadai presented the most differentiated, compared with other species, which consistented with the result summarized in the probable minimum numbers of loci and alleles analyses.

关键词： Porphyra isozyme allele gene locus genetic variety

Effect of Coconut Milk and Rare Earth on Proliferation, Rootage and isozymes of Dendrobium in Tissue Culture

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Journal of Rare Earths 2007年第S1期25卷 154-157页

作者：张子学胡能兵王伟传 Agricultural Department School of Plant Science Anhui Science and Technology University

In tissue culture, effects of coconut milk, rare earth and medium on proliferation, rootage and isozymes of Dendrobium were studied. The result indicated that coconut milk and RE could promote proliferation and rootage, with their combined treatment the most effective. In proliferation culture, coconut milk enhanced peroxidase (POD) and esterase(EST) activity and types, RE decreased POD, but increased EST activity and types and combined treatment did not show the same influence on POD and EST too. In rootage culture, adding coconut milk decreased POD activity, but improved EST activity in 1/2MS medium and declined EST activity, expression type in WPM medium. when using RE, POD and EST activity both reduced, types of the former decreased, types of the latter changed slightly with the basic medium. And combined treatment would decreased POD activity and types.

关键词： Dendrobium tissue culture coconut milk isozyme rare earths

The differenee in protein content,MDH,POD and COD isozymes during root different developmental stages in lowland rice and upland rice

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华北农学报 1993年第S2期8卷 21-27页

作者：祁忠占彭永康

The difference in protein content, MDH,POD and COD isozymes have been seenbetween lowland rice roots and upland rice roots during root growth and development. High pro-tein content has been examined in upland rice roots,but isozyme bands in lowland rice roots aremore than that in upland rice *** area of POD isozyme bands at seedling and tilleringstages of upland rice is larger than that of lowland *** POD isozymes zymogram in the rootof upland rice is more stable compared with that of lowland *** COD isozyme numbershave been showed in roots of upland rice,but the relative activity of COD isozyme in roots of up-land rice at the seedling and tillering stages is lower than that of lowland ***7 band can beshown in upland rice root at flowering stage,but it is absent in lowland ***,COD7,band can be taken as an index for resistance of the upland rice to drought environment.

关键词： upland rice lowland rice cytochrome oxidase isozyme polyacrylamide gel elec-trophoresis drought tolerance

Tissue Expression and Stock Variation of isozymes of Stone Flounder(Kareius bicoloratus)

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Journal of Ocean University of China 2007年第2期6卷 153-160页

作者： XU Jianpeng ZHANG Quanqi QI Jie WANG Zhigang Laboratory of Genetics and Breeding College of Marine Life Science Ocean University of China Qingdao 266003 P. R. China

Tissue expression and stock variation of isozymes of stone flounder （Kareius bicoloratus） were analyzed with horizontal starch gel electrophoresis. For the fourteen enzymes assayed, 31 loci were recorded. The results indicated that all the isozymes examined were obviously tissue-specific. The expressions of SOD＊, GDH＊, G3PDH-2＊ and ADH-2＊ were detected only in liver, SDH-1＊, MDH-1＊ and ADH-1＊ only in muscle, and LDH-B＊ and LDH-C＊ only in eyes. In comparison, MDH-2＊, GPI-3＊ and SDH-2＊ were detected in all tissues examined. Other loci examined were detected in a variety of tissues. Muscle and liver were selected to detect the isozyme variation of the two geographic stocks of Qingdao and Weihai, Shandong Province, China. The percentages of polymorphic loci （P0.99） were 29.17% and 25.00%, the observed heterozygosities （H0） were 0.028 ±0.014 and 0.040 ± 0.019, and the expected heterozygosities （He） were 0.039±0.017 and 0.052±0.022 in Qingdao and Wethai stock, respectively. The coefficient of gene differentiation （Fst） and genetic distance （D） between the two stocks was 0.012 and 0.0011, respectively, indicating that the genetic differentiation is low between them. Compared with other species of Pleuronectiformes, both the percentage of polymorphic loci and the mean heterozygosity ofK. Bicoloratus were at a middle level.

关键词： stone flounder Kareius bicoloratus isozyme tissue expression stock variation

Comparative Study of Immunological Properties on Glutamine Synthetase isozymes in Rice Plants

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Acta Botanica Sinica 2000年第5期42卷 471-475页

作者：林清华李常健张楚富彭进 PENGShao-Bing JohnBENNETT 武汉大学生命科学学院武汉430072 InternationalRiceResearchInstitute

In rice (Oryza sativa L.) roots two glutamine synthetase (GS) isozymes, GSra and GSrb, were identified recently in the author's experiments, but the homology of both GSra and GSrb as well as their localization in the rice roots are unclear. In the present study, the purified GSra and GSrb from rice roots were used to immunize rabbits to obtain the respective antibodies. The immunodiffusion and immunoblotting experiments showed that the antibody against GSra or GSrb was specific for GS and its isozymes. The immunoprecipitation test indicated that the antibody of GSra or GSrb not only recognized its respective antigen, but also well recognized each other's antigen. GSra or GSrb antibody recognized also better cytosolic GS1 of rice leaves, but the recognization for chloroplast GS2 from rice or spinach (Spinacia oleracea Mill.) leaves was weaker. Our results indicate that GSra and GSrb from rice roots are quite similar in antigenicity and are extremely similar proteins and that both GSra and GSrb may also be a form of cytosolic GS just as the cytosolic GS1 of rice leaves.

关键词： antigen antibody glutamine synthetase isozyme rice

THE STUDY ON ESTERASE AND PEROXIDASE isozymeS OF WHEAT LANDRACES FROM SHAANXI, CHINA

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四川农业大学学报 1992年第4期 640-645页

作者： Zuli Yang, Mingcheng Luo and Chi Yen (Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan City, Sichuan, China, Zip Code 611830)

The esterase and peroxidase isozymes of 63 landraces of wheat (Triticum aestivum L.) from Shaanxi province, China have been studied. The results showed as follows:Significant differences of either esterase or peroxidase isozymes were detected in both roots and shoots of seedling among landraces. Eand Ewere special for roots; E, E, E, Eand Eonly appeared in shoots. 2 types of isozyme patterns of esterase were involved in roots, but 4 types in shoots. No variation of peroxidase isozymes appeared in roots, but 3 variations arose in shoots. The roots showed more zymograms and more activity than the shoots in peroxidase isozyme.

关键词： isozyme Esterase peroxidase Wheat Electrophoresis

Glycolate oxidase isozymes are coordinately controlled by GLO1 and GLO4 in rice

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Glycolate oxidase isozymes are coordinately controlled by GL...

Effect of Rare Earth on Physiological-Chemical Characteristics and Its Growth of Aloe in Tissue Culture

从植物科学到农业发展——2012全国植物生物学大会

作者： Zhisheng Zhang,Yusheng Lu,Xinxiang Peng State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,College of Life Sciences,South China Agricultural University,Guangzhou 510642.

Glycolate oxidase(GLO) is a key enzyme in photorespiratory *** putative GLO genes were identified in the rice genome,but how each gene member contributes to GLO activities,particularly to its isozyme profile,is not well *** this study,we analyzed how each gene plays a role in isozyme formation and enzymatic activities in both yeast cells and rice *** GLO isozymes were detected in rice *** and GL04 are predominately expressed in rice leaves,while GL03 and GL05 are mainly expressed in the *** assays showed that all yeast-expressed GLO members except GL05 have enzymatic *** analyses suggested that GLOl,GL03 and GL04 interacted with each other,but no interactions were observed for GL05. GLO1/GL04 co-expressed in yeast exhibited the same isozyme pattern as that from rice *** either GLO1 or GLO4 was silenced,expressions of both genes were simultaneously suppressed and most of the GLO activities were lost,and consistent with this observation,little GLO isozyme protein was detected in the silenced *** contrast, no observable effect was detected when GL03 was *** analyses between the GLO isoforms expressed in yeast and the isozymes from rice leaves indicated that two of the five isozymes are homo-oligomers composed of either GLO1 or GLO4,and the other three are hetero-oligomers composed of both GLO1 and *** current data suggest that GLO isozymes are coordinately controlled by GLO1 and GLO4 in rice,and the existence of GLO isozymes and GLO molecular and compositional complexities implicate potential novel roles for GLO in plants.

关键词： Glycolate oxidase photorespiratory metabolism isozyme molecular characterization rice

来源： cnki会议评论

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Journal of Rare Earths 2005年第S1期23卷 522-525页

作者：张子学胡能兵刘侠许海平 Anhui Science and Technology University Fengyang 233100 China

In adopting tissue culture, effects of rare earth on Physiological and chemical characteristics, proliferation and rootage in aloe were studied. The results indicate that suitable concentration treatment of rare earth increases content of reducing sugar and protein, enhances activity intensity of superoxide dismutase (SOD), peroxidase (POD) isozymes and stripes of POD isozymes in the leaves. It also increases rate of proliferation and rootage respectively. The appropriate concentration of rare earth is 20～40 mg·L -1 in proliferation and rootage of aloe.

关键词： aloe reducing sugar soluble protein SOD POD isozyme tissue culture rare earths

Study of Biological Effects of Low Energy Ion Implantation on Tomato and Radish Breeding

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Plasma Science and Technology 2008年第2期10卷 254-259页

作者：梁秋霞黄群策曹刚强应芳卿刘艳波黄文 Henan Province Key Laboratory of Ion Beam Bioengineering Zhengzhou University Zhengzhou 450052 China Department of Bioengineering Zhengzhou 450001 China Zhengzhou City Institute of Vegetable Zhengzhou 450000 China

Biological effects of 30 keV low energy nitrogen ion implantation on the seeds of five types of tomato and one type of radish were investigated. Results showed that low energy ions have different effects on different vegetables. The whole dose-response curve of the germination ratio did not take on ＂the shape of saddle＂, but was a rising and falling waveform with the increase or decrease in ion implantation. In the vegetable of Solanaceae, two outstanding aberrant plants were selected from M1 of Henan No.4 tomato at a dose of 7x 1017 nitrogen ions/cm2, which had thin-leaves, long-petal and nipple tip fruit stably inherited to Mr. Furthermore the analysis of the isozyme showed that the activity of the mutant tomato seedling was distinct in quantity and color. In Raphanus sativus L., the aberrances were obvious in the mutant of radish 791 at a dose of 5×10^17 nitrogen ions/cm^2, and the weight of succulent root and the volume of growth were over twice the control＇s. At present, many species for breeding have been identified in the field and only stable species have been selected for the experiment of production. It is evident that the low energy ion implantation technology has clear effects on vegetables＇ genetic improvement.

关键词： low energy ion germination ratio dose isozyme