Comparative Study of Immunological Properties on Glutamine Synthetase Isozymes in Rice Plants

作     者：林清华 李常健 张楚富 彭进 PENGShao-Bing JohnBENNETT 

作者机构：武汉大学生命科学学院武汉430072 InternationalRiceResearchInstitute 

出 版 物：《Acta Botanica Sinica》 (Acta Botanica Sinica（植物学报：英文版）)

年 卷 期：2000年第42卷第5期

页      面：471-475页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：TheNationalNaturalScienceFoundationofChina!( 3 9870 5 2 7) CooperativeFoundationofInternationalRiceResearchInstitute 

主　　题：antigen antibody glutamine synthetase isozyme rice 

摘      要：In rice (Oryza sativa L.) roots two glutamine synthetase (GS) isozymes, GSra and GSrb, were identified recently in the author s experiments, but the homology of both GSra and GSrb as well as their localization in the rice roots are unclear. In the present study, the purified GSra and GSrb from rice roots were used to immunize rabbits to obtain the respective antibodies. The immunodiffusion and immunoblotting experiments showed that the antibody against GSra or GSrb was specific for GS and its isozymes. The immunoprecipitation test indicated that the antibody of GSra or GSrb not only recognized its respective antigen, but also well recognized each other s antigen. GSra or GSrb antibody recognized also better cytosolic GS1 of rice leaves, but the recognization for chloroplast GS2 from rice or spinach (Spinacia oleracea Mill.) leaves was weaker. Our results indicate that GSra and GSrb from rice roots are quite similar in antigenicity and are extremely similar proteins and that both GSra and GSrb may also be a form of cytosolic GS just as the cytosolic GS1 of rice leaves.