Isozyme Analysis of Jin Silver Carp (Hypophthalmichthys molitrix Var Jin)

作     者：杨蔷 郝君 鲍迪 梁爱军 金万昆 李崇文 张兴华 董仕 

作者机构：天津师范大学生命科学学院天津市细胞遗传与分子调控重点实验室天津300387 国家级天津市换新水产良种场天津301500 天津市天祥水产有限责任公司天津301500 

出 版 物：《Agricultural Science & Technology》 (农业科学与技术（英文版）)

年 卷 期：2012年第13卷第7期

页      面：1571-1574页

学科分类：0908[农学-水产] 09[农学] 

基　　金：天津市细胞遗传与分子调控重点实验室开放研究基金资助项目 天津市科技支撑计划项目(09zckfnc02100)~~ 

主　　题：Silver carp (Hypophthalmichthys molitrix) Isozyme Genetic diversity 

摘      要：[Objective] The aim was to carry out isozyme analysis of jin silver carp (Hypophthalmichthys molitrix Var Jing). [Method] The isozyme of AAT, EST, α-GPD, GPI, IDH, LDH, MDH, ME, PGM and PROT of muscles and liver in two populations of the silver carp (Hypophthalmichthys molitrix): Jin silver carp (a breed through selective breeding) and artificially propagated population bought from Jingzhou city, Hubei Province were examined by horizontal starch gel electrophoresis. [Result] Eighteen loci were observed in two populations. Two loci of GPI* and PGM* in Jing silver carp population and the locus of GPI* in Jingzhou population were polymorphic. The proportions of polymorphic loci (maximum allele frequency≤0.99) of Jing silver carp and Jingzhou populations were 11.11% and 5.56% respectively, expected heterozygosity were 0.015 0 and 0.001 1 respectively. The Nei’s genetic distances were 0.000 59 between two populations. The result of chi-square test of the GPI* gene in two populations showed that their genetic structure has very significant difference. [Conclusion] This study provided a theoretical basis for large-scale extension of Jing silver carp.