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In vitro and in vivo anticancer activity of indigofera cassioides ***. DC.

Asian Pacific Journal of Tropical Medicine 2011年第5期4卷 379-385页

作者： Raju Senthil Kumar Balasubramanian Rajkapoor Perumal Perumal Natural Products Laboratory Swamy Vivekanandha College of PharmacyTiruchengoduTamilnaduIndia Department of Pharmacology Dayanandha Sagar College of PharmacyBangaloreIndia Department of Pharmaceutical Chemistry JKK Nataraja College of PharmacyKomarapalayamTamilnaduIndia

Objective:To evaluate the antitumor,cytotoxic and antioxidant activities of methanolic leaf extract of indigofera cassioides(MEIC) against transplantable tumors and human cancer cell ***:MEIC was investigated for its short term cytotoxicity on EAC and DLA cells by trypan blue dye exclusion method and in vitro cytotoxicity on HeLa,HEp-2,HEpG-2,MCF-7, HT-29,Vero and NIH 3T3 cells by MTT *** vivo antitumor activity was studied on EAC and DLA tumor bearing *** was assessed by monitoring the mean survival time,effect on hematological parameters,antioxidant enzyme levels and solid tumor ***:MEIC exhibit potent in vitro cytotoxicity against all the tested cancer cell lines,but it was found to be safe on normal *** extract significantly(P<0.001) increase the mean survival time and also have a protective effect on the hemopoietic system at the tested dose levels(200 and 400 mg/kg). The extract prevented lipid peroxidation and restored the antioxidant enzymes catalase, superoxide dismutase,glutathione peroxidase and glutathione-s-transferase in the liver of tumor control *** also significandy(P<0.01) reduce the solid tumor ***:The results strongly support that MEIC shows potent antitumor and cytotoxic effects against EAC,DLA and human cancer cell *** extract prevents lipid peroxidation and promotes the enzymatic antioxidant defense system in tumor bearing animals which might be due to activities like scavenging of free radicals by the phytochemicals in MEIC.

关键词： indigofera cassioides Cytotoxicity Antitumor Antioxidant Mean survival time Hematological parameters Solid tumor volume

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Antioxidant activities of indigofera cassioides *** various in vitro assay models

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Asian Pacific Journal of Tropical Biomedicine 2012年第4期2卷 256-261页

作者： R Senthil Kumar B Rajkapoor P Perumal Natural Products Laboratory Swamy Vivekanandha College of Pharmacy Department of Pharmacology Dayanandha Sagar College of Pharmacy Department of Pharmaceutical Chemistry JKK Nataraja College of Pharmacy

Objective:To evaluate the antioxidant potential of methanolic leaf extract of indigofera cassioides(MEIC) using various in vilro antioxidant assay ***:Antioxidant and free radical scavenging activity of MEIC was assayed by using different in vitro models like ABTS, DPPH,nitric oxide,superoxide,hydrogen peroxide and hydroxyl *** ability of the extract was tested by the complex formation with potassium *** total phenol and flavonoid contents of the crude extract were also *** and ascorbic acid were used as ***:MEIC exhibited potent and concentration dependent free radical scavenging activity in all the tested *** ability was also found to increase with increase in MEIC *** phenol and flavonoid content determination showed that the extract is rich in phenols and ***:All the results of the in vitro antioxidant assays reveal potent antioxidant and free radical scavenging activity of the leaves of indigofera cassioides,equivalent to that of standard ascorbic acid and *** potent antioxidant activity may be attributed to its high phenolic and flavonoid contents.

关键词： indigofera cassioides Antioxidant Free radicals Oxidative stress Total reducing power Free radical scavenging activity

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Evaluation of antimicrobial activity of a lectin isolated and purified from <i>indigofera heterantha</i>

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Advances in Bioscience and Biotechnology 2013年第11期4卷 999-1006页

作者： Sakeena Qadir Ishfak Hussain Wani Shaista Rafiq Showkat Ahmad Ganie Akbar Masood Rabia Hamid Department of Biochemistry University of Kashmir Srinagar India

indigofera heterantha commonly called indigo bush is a member of leguminoseae family found in the Himalayan region of Kashmir. A lectin has been isolated from the seeds of indigofera heterantha by the purification procedure involving anion exchange chromatography on DEAE-cellulose followed by gel filtration chromatography on Sephadex G 100. Molecular characterization of the lectin was done by gel filtration and SDS-PAGE. Activity of the lectin was checked by hemagglutination assay and the sugar specificity by sugar inhibition tests. The antimicrobial activity of the purified lectin was carried out by Agar disc diffusion using appropriate standards. On the ion exchange column, the bound protein when eluted with 0-0.5 M NaCl gradient emerged as three peaks—peak I, peak II and peak III out of which only peak II showed the hemagglutinating activity. The lectin further resolved into two peaks G1 and G2 on gel filtration, with the lectin activity residing in G1, corresponding to a molecular weight of 70 KDa. The purified lectin named as indigofera heterantha Lectin (IHL) produced a single band on SDS PAGE (18 KDa), revealing the tetrameric nature of the lectin. It agglutinated human erythrocytes (A, B, AB, and O). Hemagglutination was inhibited by D-galactose, Dmannose and D-arabinose. The lectin is reasonably thermostable showing full activity within a temperature range of 30°C to 90°C. pH stability of the lectin falls in the range of 2-9. IHL demonstrated a remarkable antibacterial activity against the pathogenic bacteria Klebsiella pneumoniae, Staphylococcus aureus, Escherichia coli, and Bacillus subtilis. IHL also inhibited the growth of phytopathogenic fungi Aspergillus niger, Aspergillus oryzae and Fusarium oxysporum.

关键词： indigofera heterantha Lectin Hemagglutination Antimicrobial

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Seed Morphology of Some Species of <i>indigofera</i>(Fabaceae) from Saudi Arabia (Identification of Species and Systematic Significance)

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American Journal of Plant Sciences 2011年第3期2卷 484-495页

作者： F. A. Al-Ghamdi 不详

Seeds of 18 species of indigofera L. were examined with the scanning electron microscope and the light microscope. Macro- and micromorphological characters, including seed shape, colour, size, surface, epidermal cell shape, anticlinal boundaries, outer periclinal cell wall and relief of outer cell walls, are presented. Two types of basic anticlinal cell wall boundaries and two types of relief outer cell walls are recognized and two different shapes of the outer periclinal cell wall are described. A key for the identification of the investigated taxa based on seed characters is provided.

关键词： Seeds Morphology indigofera Fabaceae Saudi Arabia

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