In vitro and in vivo anticancer activity of Indigofera cassioides ***. DC.

作     者：Raju Senthil Kumar Balasubramanian Rajkapoor Perumal Perumal 

作者机构：Natural Products LaboratorySwamy Vivekanandha College of PharmacyTiruchengoduTamilnaduIndia Department of PharmacologyDayanandha Sagar College of PharmacyBangaloreIndia Department of Pharmaceutical ChemistryJKK Nataraja College of PharmacyKomarapalayamTamilnaduIndia 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2011年第4卷第5期

页      面：379-385页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 1002[医学-临床医学] 100602[医学-中西医结合临床] 10[医学] 

主　　题：Indigofera cassioides Cytotoxicity Antitumor Antioxidant Mean survival time Hematological parameters Solid tumor volume 

摘      要：Objective:To evaluate the antitumor,cytotoxic and antioxidant activities of methanolic leaf extract of Indigofera cassioides(MEIC) against transplantable tumors and human cancer cell ***:MEIC was investigated for its short term cytotoxicity on EAC and DLA cells by trypan blue dye exclusion method and in vitro cytotoxicity on HeLa,HEp-2,HEpG-2,MCF-7, HT-29,Vero and NIH 3T3 cells by MTT *** vivo antitumor activity was studied on EAC and DLA tumor bearing *** was assessed by monitoring the mean survival time,effect on hematological parameters,antioxidant enzyme levels and solid tumor ***:MEIC exhibit potent in vitro cytotoxicity against all the tested cancer cell lines,but it was found to be safe on normal *** extract significantly(P0.001) increase the mean survival time and also have a protective effect on the hemopoietic system at the tested dose levels(200 and 400 mg/kg). The extract prevented lipid peroxidation and restored the antioxidant enzymes catalase, superoxide dismutase,glutathione peroxidase and glutathione-s-transferase in the liver of tumor control *** also significandy(P0.01) reduce the solid tumor ***:The results strongly support that MEIC shows potent antitumor and cytotoxic effects against EAC,DLA and human cancer cell *** extract prevents lipid peroxidation and promotes the enzymatic antioxidant defense system in tumor bearing animals which might be due to activities like scavenging of free radicals by the phytochemicals in MEIC.