检索结果-南通市图书馆

Identif ication of a hypersensitive response core peptide of HrpZ and its role in increasing grape downy mildew resistance

引用

Horticultural Plant Journal 2024年第5期10卷 1112-1120页

作者： Zongbao Fan Xueqiang Guan Zhichang Zhang Yushuai Sun Fei Wang Huiru Chi Yuxin Yao State Key Laboratory of Crop Biology Collaborative Innovation Center of Fruit&Vegetable Quality and Efficient ProductionCollege of Horticulture Science and EngineeringShandong Agricultural UniversityTai'anShandong 271018China Shandong Academy of Grape/Shandong Technology Innovation Center of Wine Grape and Wine JinanShandong 250100China Zhichang Agricultural Science and Technology Development Co. Ltd.RizhaoShandong 276511China

Harpins play a key role in inducing disease resistance in crops,and identifying their core functional regions and establishing a system for their efficient expression would be very *** this study,large amounts of soluble fusion proteins of harpin HrpZ and its subpeptides were obtained via the optimized induction conditions(28℃ with 0.5 mmol·L^(-1) IPTG for 6 h)in Escherichia coli BL21(DE3).hypersensitive response(HR)assays demonstrated that the C-terminal 66 aa of HrpZ(HrpZ_C_2_2)elicited a strong HR in tobacco(Nicotiana benthamiana)and grape(Flame Seedless)***,treatment with HrpZ,and particularly HrpZ_C_2_2,significantly reduced the disease incidence and severity index of field vine leaves and those inoculated with downy *** determination of the physiological parameters indicated that HrpZ,and especially HrpZ_C_2_2,improved the photosynthesis-and chlorophyll fluorescence-related parameters,enhanced the activity of defense-related enzymes,including SOD,POD,CAT and PAL,and increased the H_(2)O_(2) ***,we efficiently expressed a core peptide of HrpZ and elucidated its strong ability to elicit a HR and resistance to downy *** research provides insight into understanding the structure and function of HrpZ and will advance the application of HrpZ_C_2_2 to increase the resistance of grapevine to downy mildew.

关键词： Grapevine Harpin HrpZ Prokaryotic expression Downy mildew hypersensitive response

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Induction of hypersensitive response and Nonhost Resistance by a Cladosporium fulvum Elicitor CfHNNI1 is Dose-Dependent and Negatively Regulated by Salicylic Acid

引用

Journal of Integrative Agriculture 2012年第10期11卷 1665-1674页

作者： XU You-ping CHEN Hui-ying ZHOU Xin CAI Xin-zhong Institute of Biotechnology College of Agriculture and Biotechnology Zhejiang University Center of Analysis and Measurement Zhejiang University Key Laboratory of Molecular Biology of Crop Pathogens and Insects Ministry of Agriculture/Zhejiang University

Nonhost resistance is a phenomenon that enables plants to protect themselves against the majority of potential pathogens, and thus has a great potential for application in plant protection. We recently found that CfHNNI1 （for Cladosporium fulvum host and nonhost plant necrosis inducer 1） is an inducer of plant hypersensitive response （HR） and nonhost resistance. In this study, its functional mechanism was analyzed. CfHNN11 was a single copy gene in C. fulvum genome. The functional ORF of the CfHNN11 cDNA was ATG3-TAG780, which showed homology with genes encoding bZIP transcription factors. The functional ORF included in frame an inner one ATG273-TAG780, which was sufficient to induce HR in plants. CfIINN11 induced plant HR in a dose-dependent manner. CfHNNIl-induced necrosis in NahG transgenic tomato plants was significantly stronger than that in their wild type controls. However, the necrosis in Nr and defl tomato mutants was similar to that in their corresponding wild type plants. These data demonstrate that induction of HR and nonhost resistance by CfHNNI1 is negatively regulated by salicylic acid signalling pathway but independent of ethylene and jasmonic acid signalling pathways.

关键词： CfHNNI1 Cladosporium fulvum ethylene hypersensitive response jasmonic acid nonhost resistance salicylic acid

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Exchangeability of Two hrp Gene Fragments from Xanthomonas oryzae *** and *** for hypersensitive response on Tobacco and Pathogenicity on Rice

引用

Agricultural Sciences in China 2003年第9期2卷 975-981页

作者： CHENGong-you 夏欣 KeyLaboratoryforMonitoringandManagementofPlantDiseasesandInsects MinistryofAgriculture~DepartmentofPlantPathologyNanjingAgriculturalUniversityNanjing210095P.R.China

hrp mutants were produced from strain JXOIII of Xanthomonas oryzae pv. oryzae (Xoo) and strain RS105 of X.o. pv. oryzicola (Xooc), respectively, by using diethyl sulfate (DES) as a mutagenic che mical. All the hrp mutants lost their pathogenicity on a susceptible host plant, rice (Shanyou63), and elicitation of the hypersensitive response (HR) on a nonhost plant, tobacco (NC89). Extracellular enzyme (amy lase, pectate lyase, proteinase, cellulase and lipase) activities of all the hrp mutants were similar to those of the corresponding wild type strains. The response of tobacco to cell sonicated integrations of the wild type strains and the hrp mutants demonstrated that there existed an HR eliciting substance which was heat stable and sensitive to protease. No HR appeared on tobacco after infiltration of the lipopolysaccharide (LPS) of both the wild strains and hrp mutants into tobacco leaves. The ability of the Xooc hrp mutants to induce HR on tobacco and cause streak disease on rice was restored by complementation with pUHRX245 from JXOIII genomic DNA library and by pUHRS138 from RS105 genomic DNA library, respectively. Subcloning of a 38.6 kb hrp fragment insert in pUHRX245 and a 39.3 kb insert in pUHRS138 revealed that a 3.3 kb Sac Ⅰ fragment from pUHRX245 and a 4.5 kb Bam HⅠ Kpn Ⅰ fragment from pUHRS138 were the minimal functional portions required for restoration of the ability of Xooc hrp mutants to induce HR on tobacco and cause disease on rice. The disease symptom caused by the conjugant (M1005 plus 3.3 kb) on rice was similar to that caused by the wild type of Xooc. It suggests that the two fragments contain the same hrp gene(s) and are responsible reciprocally for HR induction on tobacco and pathogenicity on rice.

关键词： Xanthomonas oryzae pv. oryzae X.o. pv. oryzicola hypersensitive response Pathogenicity hrp gene

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Induction of hypersensitive response and Nonhost Resistance by a Cladosporium fulvum elicitor CfHNNI1 is Dose-dependent and Negatively Regulated by Salicylic Acid

Induction of Hypersensitive Response and Nonhost Resistance ...

引用

中国植物病理学会2012年学术年会

作者： XU You-Ping~(1.2) CHEN Hui-Ying~1 LU Lin-Hui~1 ZHOU Xin~1 CAI Xin-Zhong~(1**) (1.Institute of Biotechnology,College of Agriculture and Biotechnology, Zhejiang University,Hangzhou 310058,.China 2.Center of Analysis and measurement,Zhejiang University,Hangzhou 310058,.China)

＜正＞Nonhost resistance is a phenomenon that enables plants to protect themselves against the majority of potential pathogens,and thus has a great potential for application in plant *** recently found that CfHNNI1（for Cladosporium fulvum host and nonhost plant necrosis inducer 1 ） is an inducer of plant hypersensitive response（HR） and nonhost *** this study,its functional mechanism was ***1 was a single copy gene in *** *** functional ORF of the CfHNNI1 cDNA was ATG3～TAG780,which shown homology with genes encoding bZIP transcription factors. The functional ORF included in frame an inner one ATG273～TAG780,which was sufficient to induce HR in ***1 induced plant HR in a dose-dependent ***1-induced necrosis in NahG transgenic tomato plants was significantly stronger than in their wild type ***,that in Nr and defl tomato mutants was similar to that in their corresponding wild type *** data demonstrate that induction of HR and nonhost resistance by CfHNNI1 is negatively regulated by salicylic acid signalling pathway but independent of ethylene and jasmonic acid signalling pathways.

关键词： CfHNNI1 Cladosporium fulvum ethylene hypersensitive response jasmonic acid nonhost resistance salicylic acid

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Identification of Genes Required for Cf-dependent hypersensitive response

Identification of Genes Required for Cf-dependent hypersensi...

引用

中国植物病理学会2012年学术年会

作者： XU Qiu-Fang~1 CHENG Wei-Shun~1 LI Shuang-Sheng~1 LI Wen~1 ZHANG Zhi-Xin~1 XU You-Ping~2 CAI Xin-Zhong~(1**) (1.Institute of Biotechnology,College of Agriculture and Biotechnology,Zhejiang University, 866 Yu Hang Tang Road,Hangzhou 310058,China 2.Center of Analysis and measurement, Zhejiang University,866 Yu Hang Tang Road,Hangzhou 310058,China

＜正＞Identification of hypersensitive response（HR） regulators is essential to dissect the molecular mechanisms underlying plant disease *** this study,combined proteomics and RNA interfering analyses were employed to identify genes required for the HR conferred by the tomato resistance gene Cf-4 and the Cladosporium fulvum avirulence gene *** nine proteins differentially expressed in the tomato seedlings mounting and those not mounting the Cf-4/Avr4-dependent HR were identified through proteomics *** them were a variety of defence-related proteins including a cysteine protease Pipl,an operative target of another *** effector ***,glutathione-mediated antioxidation is a major response to the Cf-4/Avr4-dependent *** analysis through Tobacco rattle virus-induced gene silencing and transient RNAi assays of the chosen sixteen differentially expressed proteins revealed that seven genes,which encode Pipl homolog NbPipl,a SIPK type MAP kinase Nbf4,an asparagine synthetase NbAsn,a trypsin inhibitor LeMir-like protein NbMir,a small GTP- binding protein,a late embryogenesis-like protein and an ASR4-like protein,were required for the Cf- 4/Avr4-dependent ***,the former four genes were essential for the Cf-9/Avr9-dependent HR;NbPipl,NbAsn and NbMir but not NbfA affected a nonadaptive bacterial pathogen Xanthomonas oryzae ***-induced HR as well in Nicotiana *** data demonstrate that Pipl and LeMir may play a general role in HR and plant immunity,and application of combined proteomics and RNA interfering analyses is an efficient strategy to identify genes required for HR,disease resistance and probably other biological processes in plants.

关键词： Cf Cladosporium fulvum hypersensitive response defence gene silencing proteomics regulation resistance RNAi

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Maize homologs of COMT,a key enzyme in lignin biosynthesis,modulate the hypersensitive response mediated by resistance protein Rp1-D21

Maize homologs of COMT,a key enzyme in lignin biosynthesis,m...

引用

中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者：刘孟洁 Sun Yang Luan Qingling Liu Xiaoying Wang Mei Zhu Yuxiu Mao Weichao 王官锋 The Key Laboratory of Plant Cell Engineering and Germplasm Innovation Ministry of EducationCollege of Life SciencesShandong University

Rp1-D21,a naturally occurring mutant in maize,derives from an intragenic recombination event between two CC-NB-LRR genes,Rp1-D and ***-D21 induces an auto-active hypersensitive response(HR) in maize and Nicotiana benthamiana in the absence of pathogen infection,which has been used as a powerful tool to investigate the genes function in modulating the HR mediated by *** plays significant roles in plant development and defense and multiple key enzymes in lignin biosynthesis pathway have been reported to be involved in response to pathogen *** our previous studies,hydroxycinnamoyltransferase(HCT) and caffeoyl-CoA O-methyltransferase(CCoAOMT),two key enzymes in lignin biosynthesis,have been proved to suppress Rpl-D21-mediated HR through physical *** acid O-methyltransferase(COMT) is another key enzyme in lignin biosynthesis and the well-known bm3(brown midrib 3) mutant in maize is due to the disruption of *** investigate the function of COMT in Rpl-D21-mediated HR,two homologs of COMT(COMT1 and COMT2) were transient co-expression with Rpl-D21 or its functional signaling domain coiled cob(CC)in *** results showed that COMT2,but not COMT1 suppressed both Rpl-D21 and CC mediated ***-IP assay showed that COMT2 interacts with CC in ***,COMT2,but not COMT1 interacts with HCT and CCoAOMT,suggesting these three proteins might form a complex in *** results indicate that some homologs of key enzymes in lignin biosynthesis may be synergetic to regulate Rp1-D21-mediated *** study will clarify the regulatory function of maize COMT homologs in defense response and it is potential to use COMT1 as a new gene resource for maize genetic improvement.

关键词： Maize hypersensitive response Rp1-D21 COMT

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Maize metacaspase inhibits the hypersensitive response induced by autoactive NLR protein Rp1-D21

Maize metacaspase inhibits the hypersensitive response induc...

引用

中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者：栾庆玲 Liu Mengjie Sun Yang Wang Mei Liu Xiaoying Zhu Yuxiu Mao Weichao 王官锋 The Key Laboratory of Plant Cell Engineering and Germplasm Innovation Ministry of EducationCollege of Life ScienceShandong University

Programmed cell death(PCD) plays important roles in plant innate *** disease resistance(R) genes encode nucleotide binding leucine-rich-repeat(NLR) proteins that trigger a rapid localized PCD termed the hypersensitive response(HR) upon pathogen *** maize NLR protein Rpl-D21 derives from an intragenic recombination between two NLRs,Rpl-D and Rpl-dp2,and confers an autoactive HR in the absence of pathogen ***(MC),the distant homolog of caspase in the caspase-like domain(CD) cysteine protease superfamily,is widely distributed in different species and plays important roles in *** maize,MC can be divided into two types,type Ⅰ and type Ⅱ.Phylogenetic analysis showed that eight genes encode type Ⅰ(ZmMC 1-8) and three genes encode type Ⅱ(ZmMC9-11).Both types of MC contain two conserved caspase-like catalytic domains,P20(20 kDa) and P10(10 kDa).Two types of MC differ in their N-terminal pro-domains and the length of the linker between P20 and *** our RNA-seq data,we found that several ZmMC genes are differentially expressed in Rp1-D21 mutant compared to wild *** transiently co-expressed ZmMC and Rpl-D21 in *** and found that type Ⅰ ZmMC1 and ZmMC2 suppress Rp1-D21-mediated HR,while type Ⅱ ZmMC9 has no obvious *** suppression function of ZmMC1 and ZmMC2 is independent of the putative catalytic residues and the N-terminal *** results suggest that two types of MC have different function in regulating NLR-mediated *** molecular mechanism will be further explored.

关键词： NLR Rp1-D21 Metacaspase hypersensitive response

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

ANNEXIN 8 negatively regulates RPW8.1-mediated cell death and disease resistance in Arabidopsis

引用

Journal of Integrative Plant Biology 2021年第2期63卷 378-392页

作者： Zhi-Xue Zhao Yong-Ju Xu Yang Lei Qin Li Ji-Qun Zhao Yan Li Jing Fan Shunyuan Xiao Wen-Ming Wang Rice Research Institute and Key Laboratory for Major Crop Diseases Sichuan Agricultural UniversityChengdu 611130China Institute for Bioscience and Biotechnology Research&Department of Plant Science and Landscape Architecture University of MarylandRockvilleMaryland 20850USA

Study on the regulation of broad-spectrum resistance is an active area in plant biology. RESISTANCE TO POWDERY MILDEW 8.1(RPW8.1)is one of a few broad-spectrum resistance genes triggering the hypersensitive response(HR) to restrict multiple pathogenic infections. To address the question how RPW8.1 signaling is regulated,we performed a genetic screen and tried to identify mutations enhancing RPW8.1-mediated ***, we provided evidence to connect an annexin protein with RPW8.1-mediated resistance in Arabidopsis against powdery mildew. We isolated and characterized Arabidopsis b7-6 mutant.A point mutation in b7-6 at the At5 g12380 locus resulted in an amino acid substitution in ANNEXIN8(AtANN8). Loss-of-function or RNA-silencing of AtANN8 led to enhanced expression of RPW8.1,RPW8.1-dependent necrotic lesions in leaves, and defense against powdery mildew. Conversely,over-expression of AtANN8 compromised RPW8.1-mediated disease resistance and cell death. Interestingly, the mutation in AtANN8 enhanced RPW8.1-triggered H2O2. In addition, mutation in AtANN8 led to hypersensitivity to salt stress. Together, our data indicate that AtANN8 is involved in multiple stress signaling pathways and negatively regulates RPW8.1-mediated resistance against powdery mildew and cell death, thus linking ANNEXIN’s function with plant immunity.

关键词： AtANN8 cell death disease resistance H2O2 hypersensitive response powdery mildew RPW8.1

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

OsbZIP53 Negatively Regulates Immunity response by Involving in Reactive Oxygen Species and Salicylic Acid Metabolism in Rice

引用

Rice science 2024年第2期31卷 190-202,I0022-I0028页

作者： WU Lijuan HAN Cong WANG Huimei HE Yuchang LIN Hai WANG Lei CHEN Chen E Zhiguo State Key Laboratory of Rice Biology and Breeding China National Rice Research InstituteHangzhou 310006China Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops Agricultural College of Yangzhou UniversityYangzhou 225009China

The basic region/leucine zipper(bZIP)transcription factors play important roles in plant development and responses to abiotic and biotic ***53 regulates resistance to Magnaporthe oryzae in rice by analyzing APIP5-RNAi transgenic *** further investigate the biological functions of OsbZIP53,we generated osbzip53 mutants using CRISPR/Cas9 editing and also constructed OsbZIP53 over-expression transgenic *** analysis of phenotypical,physiological,and transcriptional data showed that knocking-out OsbZIP53 not only improved disease resistance by inducing a hypersensitivity response in plants,but also regulated the immune response through the salicylic acid ***,disrupting OsbZIP53 increased H2O2 accumulation by promoting reactive oxygen species generation through up-regulation of several respiratory burst oxidase homologs(Osrboh genes)and weakened H2O2 degradation by directly targeting *** addition,the growth of osbzip53 mutants was seriously impaired,while OsbZIP53 over-expression lines displayed a similar phenotype to the wild type,suggesting that OsbZIP53 has a balancing effect on rice immune response and growth.

关键词： OsbZIP53 hypersensitive response reactive oxygen species metabolism rice immunity salicylic acid transcription factor

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Xoryp_08180 of Xanthomonas oryzae ***,Encoding a Hypothetical Protein,is Regulated by HrpG and HrpX and Required for Full Virulence in Rice

引用

Journal of Integrative Agriculture 2012年第4期11卷 600-610页

作者： SHEN Yi-ping ZOU Li-fang LI Yu-rong ZOU Hua-song LIU Xi-ling CHEN Gong-you School of Agriculture and Biology Shanghai Jiao Tong University/Key Laboratory of Urban （South） Ministry of Agriculture Shanghai 200240 P.R. China Department of Plant Protection Nanjing Agricultural University/Key Laboratory of Integrated Management of Crop Diseases and Pests Ministry of Education Nanjing 210095 P.R. China

Xanthomonas oryzae ***（Xoc） causes a destructive bacterial leaf streak disease in *** of the gene products annotated as hypothetical proteins in the genome of Xoc may contribute to its virulence in rice.A mutant,Mxoc1679,screened from our previous Tn5-tagged mutant library for Xoc strain RS105,showed reduced virulence in *** this mutant,a gene named as Xoryp_08180 was disrupted by Tn5 ***_08180 encodes a 1 306-aa hypothetical protein which is highly conserved in Xanthomonas ***-polar mutation of Xoryp_08180 in RS105 strain led to a significant reduction in bacterial virulence and growth in rice,a delayed hypersensitive response（HR） in non-host tobacco,and a decrease in extracellular protease *** deficiencies above were restored to wild-type level in the complementary strain by expressing Xoryp_08180 in *** addition,the expression of Xoryp_08180 was repressed in hrpG and hrpX mutants in planta but not in a nutrient-rich *** results suggested that Xoryp_08180 is a virulence factor required for extracellular protease production,HR induction and full virulence of Xoc.

关键词： Xanthomonas oryzae pv.oryzicola Xoryp_08180 hypothetical protein virulence hypersensitive response extracellular protease

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：