Xoryp_08180 of Xanthomonas oryzae ***,Encoding a Hypothetical Protein,is Regulated by HrpG and HrpX and Required for Full Virulence in Rice

作     者：SHEN Yi-ping ZOU Li-fang LI Yu-rong ZOU Hua-song LIU Xi-ling CHEN Gong-you 

作者机构：School of Agriculture and Biology Shanghai Jiao Tong University/Key Laboratory of Urban （South） Ministry of Agriculture Shanghai 200240 P.R. China Department of Plant Protection Nanjing Agricultural University/Key Laboratory of Integrated Management of Crop Diseases and Pests Ministry of Education Nanjing 210095 P.R. China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2012年第11卷第4期

页      面：600-610页

核心收录：

学科分类：0906[农学-兽医学] 09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治] 

基　　金：supported by the National Natural Science Foundation of China(31071656,31000071) the National Transgenic Major Program,China(2008ZX08001-002) the Special Fund for Agro-scientific Research in the Public Interest,China(NYHYZX07-056) 

主　　题：Xanthomonas oryzae pv.oryzicola Xoryp_08180 hypothetical protein virulence hypersensitive response extracellular protease 

摘      要：Xanthomonas oryzae ***（Xoc） causes a destructive bacterial leaf streak disease in *** of the gene products annotated as hypothetical proteins in the genome of Xoc may contribute to its virulence in rice.A mutant,Mxoc1679,screened from our previous Tn5-tagged mutant library for Xoc strain RS105,showed reduced virulence in *** this mutant,a gene named as Xoryp_08180 was disrupted by Tn5 ***_08180 encodes a 1 306-aa hypothetical protein which is highly conserved in Xanthomonas ***-polar mutation of Xoryp_08180 in RS105 strain led to a significant reduction in bacterial virulence and growth in rice,a delayed hypersensitive response（HR） in non-host tobacco,and a decrease in extracellular protease *** deficiencies above were restored to wild-type level in the complementary strain by expressing Xoryp_08180 in *** addition,the expression of Xoryp_08180 was repressed in hrpG and hrpX mutants in planta but not in a nutrient-rich *** results suggested that Xoryp_08180 is a virulence factor required for extracellular protease production,HR induction and full virulence of Xoc.