Maize metacaspase inhibits the hypersensitive response induced by autoactive NLR protein Rp1-D21
作者单位：The Key Laboratory of Plant Cell Engineering and Germplasm InnovationMinistry of EducationCollege of Life ScienceShandong University
会议名称：《中国植物病理学会第十一届全国会员代表大会暨2018年学术年会》
会议日期：2018年
关 键 词：NLR Rp1-D21 Metacaspase Hypersensitive response
摘 要：Programmed cell death(PCD) plays important roles in plant innate *** disease resistance(R) genes encode nucleotide binding leucine-rich-repeat(NLR) proteins that trigger a rapid localized PCD termed the hypersensitive response(HR) upon pathogen *** maize NLR protein Rpl-D21 derives from an intragenic recombination between two NLRs,Rpl-D and Rpl-dp2,and confers an autoactive HR in the absence of pathogen ***(MC),the distant homolog of caspase in the caspase-like domain(CD) cysteine protease superfamily,is widely distributed in different species and plays important roles in *** maize,MC can be divided into two types,type Ⅰ and type Ⅱ.Phylogenetic analysis showed that eight genes encode type Ⅰ(ZmMC 1-8) and three genes encode type Ⅱ(ZmMC9-11).Both types of MC contain two conserved caspase-like catalytic domains,P20(20 kDa) and P10(10 kDa).Two types of MC differ in their N-terminal pro-domains and the length of the linker between P20 and *** our RNA-seq data,we found that several ZmMC genes are differentially expressed in Rp1-D21 mutant compared to wild *** transiently co-expressed ZmMC and Rpl-D21 in *** and found that type Ⅰ ZmMC1 and ZmMC2 suppress Rp1-D21-mediated HR,while type Ⅱ ZmMC9 has no obvious *** suppression function of ZmMC1 and ZmMC2 is independent of the putative catalytic residues and the N-terminal *** results suggest that two types of MC have different function in regulating NLR-mediated *** molecular mechanism will be further explored.