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Mol.cul.r identification of sex in hippophae rhamnoides l. using isozyme and RAPD markers

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Forestry Studies in China 2010年第2期12卷 62-66页

作者： Amit SHARMA Gaurav ZINTA Satender RANA Poonam SHIRKOT Department of Biotechnol.gy Dr. Yashwant Singh Parmar University of Horticulture & Forestry

In many dioecious pl.nts, gender affects economic val.e, breeding schemes and opportunities for commercial.harvests. hippophae rhamnoides l. is a dioecious pl.nt species in which femal. genotypes are commercial.y preferred over mal. genotypes. Its berries have rich medicinal. nutritional.and pharmaceutical.properties because of their l.rge amounts of vitamins, essential.oil., proteins, fatty acids, free amino acids and fl.vanoids. Primary l.mitation for breeding H. rhamnoides l. is its dioecious nature, since gender cannot be identified by traditional.methods. Therefore, some rel.abl. and quick methods need to be devel.ped. This commu- nication deal. with the devel.pment of isozyme and RAPD markers for earl. sex identification in this dioecious tree. The isozyme anal.sis was conducted with four enzyme systems, viz. peroxidase, esterase, mal.te dehydrogenase and catal.se. The peroxidase enzyme system produced a femal. specific sex marker, which successful.y differentiated between the staminate and pistil.ate geno- types ofH. rhamnoides l. Thirty five random decamer primers were used in our study and one mal. sex l.nked marker was identified. OPD-20 （5＇-ACTTCGCCAC-3＇） displ.yed a band at 911 bp that expressed pol.morphism between mal. and femal. genotypes. The staminate and pistil.ate genotypes coul. be distinguished using RAPD marker OPD-209n. These resul.s reveal.d the immense poten- tial.of peroxidase isozyme patterns and RAPD as genetic markers for sex identification in H. rhamnoides l.

关键词： hippophae rhamnoides l. sex markers isozyme RAPD dioecism

Comparative quantitative anal.sis of fruit oil.from hippophae rhamnoides(seabuckthorn) by qNMR, FTIR and GC–MS

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Chinese Herbal.Medicines 2023年第4期15卷 607-613页

作者： Dattatraya Dinkar Gore Furkan Ahmad Kul.hushan Tikoo Arvind Kumar Bansal.Dinesh Kumar Inder Pal.Singh National.Institute of Pharmaceutical.Education and Research(NIPER) S.A.S.Nagar 160062India CSIR-Institute of Himal.yan Bioresource and Technol.gy Palampur 176061India

Objective: To devel.p a qNMR method for quantitative anal.sis of triacyl.l.cerol. in fruit oil.of hippophae rhamnoides(seabuckthorn, SBT) and anal.ze commercial.sampl.s of SBT oil. using GC–MS and ***: SBT fruit oil.IPHRFH) was extracted with hexane and the trigl.ceride(TAG) was isol.ted by vacuum l.quid chromatography. Six different branded SBT oil. purchased from e-commerce suppl.ers(Amazon) and in-house prepared SBT oil.was anal.zed by qNMR and fatty acyl.composition of TAGs determined by using NMR. In-house oil.was al.o anal.sed by GC–MS and FTIR ***: The qNMR resul.s showed that the oil.contained 80.3% of triacyl.l.cerol.TAG). The SBT oil.TAGs comprised of l.nol.nate 6.6%, pal.itol.ate/ol.ate 65.4%, and total.saturated fatty acyl.chain incl.ding pal.itate 28% as determined by qNMR. GC–MS anal.sis reveal.d that the major acyl.functional.ties present in the TAG were pal.itol.ic acid 36.5%, ol.ic acid 12.9%, pal.itic acid 21.2%, and l.nol.ic acid 18%. Of the six commercial.sampl.s anal.zed, sampl.s from onl. one suppl.er(SW) were fruit oil.Al. others were the seed oil. or mix of fruit oil.and seed oil. The l.bel. for sampl.s except for the SW did not indicate whether it was fruit oil.or seed ***: The resul.s suggest that SBT oil.shoul. be anal.zed by combination of GC–MS, FTIR and qNMR for factual.content of free fatty acid or TAGs, which are chemical.y different in nature and affect the qual.ty of oil. GC–MS showed the content of omega free fatty acids after hydrol.sis, whil. qNMR and FTIR showed the content of TAGs. The major acyl.functional.ties found in SBT fruit oil.TAGs are pal.itol.ate/pal.itate/ol.ate, whil. l.nol.ate and l.nonel.te make up a minor fraction. Furthermore,anal.sis of commercial.sampl.s showed discrepancies between l.bel.cl.ims and actual.content.

关键词： hippophae rhamnoides l. omega fatty acids qNMR seabuckthorn triacyl.l.cerol.

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沙棘硬枝与嫩枝扦插扩繁技术

林业科技通讯 2022年第9期 24-26页

作者：郭建斌段爱国罗红梅彭瑞吴建平张建国中国林学会北京100091 国家林业和草原局林木培育重点实验室中国林业科学研究院林业研究所北京100091 赤峰市敖汉旗林业局内蒙古赤峰024329 不详

对大果沙棘(hippophae rhamnoides l.)引进、杂交选育的沙棘优良品系的硬枝扦插和嫩枝扦插育苗技术进行了总结,分析了不同栽培区不同沙棘品系扦插繁殖的技术要点,发现硬枝扦插生根率在50%以上,嫩枝扦插生根率在不同试点连续多年均达75%... 详细信息

对大果沙棘(hippophae rhamnoides l.)引进、杂交选育的沙棘优良品系的硬枝扦插和嫩枝扦插育苗技术进行了总结,分析了不同栽培区不同沙棘品系扦插繁殖的技术要点,发现硬枝扦插生根率在50%以上,嫩枝扦插生根率在不同试点连续多年均达75%以上;嫩枝扦插苗的移栽环节非常关键,要及时移栽;不同品种嫩枝生根能力的差异主要是品种的遗传特性和插穗的差异造成的;插穗的下部径的粗细与生根能力密切相关,建议采集的新梢插穗下部径应不小于0.3cm。

关键词：沙棘 hippophae rhamnoides l. 良种硬枝扦插嫩枝扦插生根能力

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沙棘良种‘红棘1号’快速繁育技术

林业科技通讯 2024年第1期 100-101页

作者：周闯阜新市林业服务中心辽宁阜新123000

‘红棘1号’沙棘(hippophae rhamnoides l.)于2022年通过了国家林业和草原局的良种审定,被确定为林木良种用于林业生产。‘红棘1号’沙棘快速繁育技术,有利于沙棘产业的快速发展。本文从苗床建立、抽穗制备、扦插与苗床管理、移植等4个... 详细信息

‘红棘1号’沙棘(hippophae rhamnoides l.)于2022年通过了国家林业和草原局的良种审定,被确定为林木良种用于林业生产。‘红棘1号’沙棘快速繁育技术,有利于沙棘产业的快速发展。本文从苗床建立、抽穗制备、扦插与苗床管理、移植等4个方面进行了系统阐述,为沙棘良种嫩枝扦插实现规范化和工厂化育苗提供技术支撑。

关键词：沙棘 hippophae rhamnoides l. ‘红棘1号’ 扦插育苗