Molecular identification of sex in Hippophae rhamnoides L. using isozyme and RAPD markers

作     者：Amit SHARMA Gaurav ZINTA Satender RANA Poonam SHIRKOT 

作者机构：Department of BiotechnologyDr. Yashwant Singh Parmar University of Horticulture & Forestry 

出 版 物：《Forestry Studies in China》 (中国林学（英文版）)

年 卷 期：2010年第12卷第2期

页      面：62-66页

学科分类：0907[农学-林学] 08[工学] 0829[工学-林业工程] 09[农学] 

基　　金：The first author gratefully acknowledges the help rendered by Dr. S. K. Pandey  Director CPRI  Shimla (HP) for providing space and technical guidance 

主　　题：Hippophae rhamnoides L. sex markers isozyme RAPD dioecism 

摘      要：In many dioecious plants, gender affects economic value, breeding schemes and opportunities for commercial harvests. Hippophae rhamnoides L. is a dioecious plant species in which female genotypes are commercially preferred over male genotypes. Its berries have rich medicinal, nutritional and pharmaceutical properties because of their large amounts of vitamins, essential oils, proteins, fatty acids, free amino acids and flavanoids. Primary limitation for breeding H. rhamnoides L. is its dioecious nature, since gender cannot be identified by traditional methods. Therefore, some reliable and quick methods need to be developed. This commu- nication deals with the development of isozyme and RAPD markers for early sex identification in this dioecious tree. The isozyme analysis was conducted with four enzyme systems, viz. peroxidase, esterase, malate dehydrogenase and catalase. The peroxidase enzyme system produced a female specific sex marker, which successfully differentiated between the staminate and pistillate geno- types ofH. rhamnoides L. Thirty five random decamer primers were used in our study and one male sex linked marker was identified. OPD-20 （5＇-ACTTCGCCAC-3＇） displayed a band at 911 bp that expressed polymorphism between male and female genotypes. The staminate and pistillate genotypes could be distinguished using RAPD marker OPD-209n. These results revealed the immense poten- tial of peroxidase isozyme patterns and RAPD as genetic markers for sex identification in H. rhamnoides L.