检索结果-南通市图书馆

erythropoietin ameliorates early ischemia-reperfusion injury following the Pringle maneuver

World Journal of Gastroenterology 2010年第38期16卷 4838-4845页

作者： Masato Kato Tokihiko Sawada Junji Kita Mitsugi Shimoda Keiichi Kubota Second Department of Surgery Dokkyo Medical UniversityKitakobayash 880

AIM:To investigate the protective effect of erythropoietin (Epo) against ischemia-reperfusion injury (IR/I) following the Pringle maneuver (PM),in comparison with conventional steroid administration in a prospective randomized trial. METHODS:Patients were randomized by age, sex, diagnosis, and surgical method, and assigned to three groups:(1) A steroid group (STRD, n= 9) who received 100 mg of hydrocortisone before PM, and on postoperative days 1, 2 and 3, followed by tapering until postoperative day 7; (2) An EPO1 group (n=10) who received 30 000 U of Epo before the PM and at the end of surgery; and (3) An EPO2 group (n=8) who received 60 000 U of Epo before the PM. Hemoglobin (Hb), hematocrit (Ht), aspartate aminotransferase (AST), alanine transaminase (ALT),lactate dehydrogenase (LDH), lactate, interleukin-6 (IL-6),and tumor necrosis factor(TNF)-α were measured before and just after (Day 0) surgery, and on postoperative days 1, 3, 7 and 14. RESULTS: There were no increases in Hb and Ht in the EPO1 and EPO2 groups. AST was signif icantly lower in EPO1 than in STRD on Day 0 (P=0.041), and lower in EPO1 than in STRD and EPO2 on Day 1 (P=0.018). ALT was signif icantly lower in EPO1 than in STRD and EPO2 on Day 0 (P=0.020) and Day 1 (P=0.004). There were no signif icant inter-group differences in the levels of LDH and lactate. IL-6 was signif icantly lower in EPO1 than in STRD and EPO2 on Day 0 (P=0.0036) and Day 1 (P=0.0451). TNF-α was signif icantly lower in EPO1 than in STRD and EPO2 on Day 0 (P=0.0006) and Day 1 (P<0.0001). Furthermore, hospitalization was signif icantly shorter in EPO1 and EPO2 than in ***:Epo has greater potential than steroids to ameliorate IR/I after the PM. Epo at a dose of 30000 U, administered before PM and just after surgery, yields better results.

关键词： erythropoietin Hepatic resection Pringle maneuver Steroid Prospective randomized study

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

erythropoietin upregulates growth associated protein-43 expression and promotes retinal ganglion cell axonal regeneration in vivo after optic nerve crush

引用

Neural Regeneration Research 2012年第4期7卷 295-301页

作者： Haibo Tan Xin Kang Yisheng Zhong Xi Shen Yu Cheng Qin Jiao Lianfu Deng Department of Ophthalmology Ruijin Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200025 China Department of Clinical Pharmacology Changhai Hospital Affiliated to Second Mifitary Medical University Shanghai 200433 China Shanghai Institute of Traumatology and Orthopedics Shanghai 200025 China

In this study, we established a rat model of optic nerve crush to explore the effects of erythropoietin on retinal ganglion cell axonal regeneration. At 15 days after injury in erythropoietin treated rats, retinal ganglion cell densities in regions corresponding to the 1/6, 3/6 and 5/6 ratios of the retinal radius were significantly increased. In addition, the number of growth associated protein-43 positive axons was significantly increased at different distances （50, 250 and 500 pm） from the crush site after erythropoietin treatment. erythropoietin significantly increased growth associated protein-43 protein levels in the retina after crush injury, as determined by westem blot and immunofluorescence analysis. These results demonstrate that erythropoietin protects injured retinal ganglion cells and promotes axonal regeneration.

关键词： erythropoietin retinal ganglion cells axonal regeneration optic nerve crush neural regeneration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

erythropoietin -induced proliferation of gastric mucosal cells

引用

World Journal of Gastroenterology 2006年第2期12卷 234-239页

作者： Kazuro Itoh Yoshio Sawasaki Kyoko Takeuchi Shingo Kato Nobuhiro Imai Yoichiro Kato Noriyuki Shibata Makio Kobayashi Yoshiyuki Moriguchi Masato Higuchi Fumio Ishihata Yushi Sudoh Soichiro Miura Second Department of Internal Medicine National Defense Medical CollegeTokorozawaSaitamaJapan First Department of Anatomy National Defense Medical CollegeTokorozawaSaitamaJapan Department of Pathology Tokyo Women's Medical CollegeShinjuku-kuTokyoJapan Chugai Pharmaceutical Co. Ltd.GotenbaShizuokaJapan Kisen Hospital Katsushika-kuTokyoJapan

AIM： To analyze the localization of erythropoietin receptor on gastric specimens and characterize the effects of erythropoietin on the normal gastric epithelial proliferation using a porcine gastric epithelial cell culture model. METHODS： erythropoietin receptor was detected by RT-PCR, Western blotting and immunohistochermistry. Growth stimulation effects of erythropoietin on cultured gastric mucosal cells were determined by ELISA using bromodeoxyuridine （BrdU）. RESULTS： erythropoietin receptor was detected on cultured porcine gastric mucosal epithelial cells. erythropoietin receptor was also detected histochemically at the base of gastric mucosal epithelium. BrdU assay demonstrated a dose-dependent increase in growth potential of cultured porcine gastric mucosal epithelial cells by administration of erythropoietin, as well as these effects were inhibited by administration of antierythropoietin antibody （P〈 0.01）. CONCLUSION： These findings indicate that erythropoietin has a potential to proliferate gastric mucosal epithelium via erythropoietin receptor.

关键词： erythropoietin erythropoietin receptor Gastric epithelial cell proliferation Porcine gastric mucosal epithelial cells

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

erythropoietin gene transfer into rat testes by in vivo electropo-ration may reduce the risk of germ cell loss caused by cryptorchidism

引用

Asian Journal of Andrology 2005年第4期7卷 369-373,页

作者： Masaki Dobashi Kazumasa Goda Hiroki Maruyama Masato Fujisawa Division of Urology Department of Organs Therapeutics Faculty of Medicine Kobe University Graduate School of Medicine Kobe 650-0017 Japan Division of Nephrology and Rheumatology Niigata University Graduate School of Medical and Dental Sciences Niigata 951-8120 Japan

Aim： To investigate the effects of rat erythropoietin （Epo） on spermatogenesis by transferring rat Epo gene into cryptorchid testes by means of in vivo electroporation. Methods： Sprague-Dawley rats with surgically-induced unilateral cryptorchidism were divided into three groups： the first group was given intratesticular injections of pCAGGS-Epo （pCAGGS-Epo group）, the second group was given intratesticular injections of pCAGGS （pCAGGS group）, and the third group were given intratesticular injections of phosphate-buffered saline （PBS group）. At the same time, square electric pulses of 30 V were applied six times with a time constant of 100 ms. One or two weeks after injection, each testis was weighed and the ratio of the total number of germ cells to that of Sertoli cells （G/S ratio） was calculated to evaluate the impairment of spermatogenesis. Ten testes taken from each of the three groups were examined at each time point. Results： The testicular weight after the injection of pCAGGS-Epo or pCAGGS control plasmid was （0.85 ± 0.08） g and （0.83 ± 0.03） g, respectively, at week 1 （P = 0.788） and （0.62 ± 0.06） g and （0.52 ± 0.02） g, respectively, at week 2 （P = 0.047）. At week 1, spermatids and sperm were more abundant in testes with pCAGGS-Epo than those in the control testes. At week 2, spermatids and sperm were hardly detected in either group. The G/S ratio was 23.27 ± 6.80 vs. 18.63 ± 5.30 at week 1 （P = 0.0078） and 7.16 ± 3.06 vs. 6.05 ± 1.58 at week 2 （P = 0.1471）, respectively. Conclusion： The transfer of Epo to rat testes by in vivo electroporation may reduce the risk of the germ cell loss caused by cryptorchidism.

关键词： electroporation gene transfer techniques erythropoietin spermatogenesis Epo

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

erythropoietin reduces apoptosis of brain tissue cells in rats after cerebral ischemia/reperfusion injury:a characteristic analysis using magnetic resonance imaging

引用

Neural Regeneration Research 2016年第9期11卷 1450-1455页

作者： Chun-juan Jiang Zhong-juan Wang Yan-jun Zhao Zhui-yang Zhang Jing-jing Tao Jian-yong Ma Department of Radiology Wuxi Second People's Hospital Affiliated to Nanjing Medical University

Some in vitro experiments have shown that erythropoietin （EPO） increases resistance to apoptosis and facilitates neuronal survival follow- ing cerebral ischemia. However, results from in vivo studies are rarely reported. Perfusion-weighted imaging （PWI） and diffusion-weighted imaging （DWI） have been applied successfully to distinguish acute cerebral ischemic necrosis and penumbra in living animals; therefore, we hypothesized that PWI and DWI could be used to provide imaging evidence in vivo for the conclusion that EPO could reduce apoptosis in brain areas injured by cerebral ischemia/reperfusion. To validate this hypothesis, we established a rat model of focal cerebral ischemia/ reperfusion injury, and treated with intra-cerebroventricular injection of EPO （5,000 U/kg） 20 minutes before injury. Brain tissue in the ischemic injury zone was sampled using MRI-guided localization. The relative area of abnormal tissue, changes in PWI and DWI in the ischemic injury zone, and the number of apoptotic cells based on TdT-mediated dUTP-biotin nick end-labeling （TUNEL） were assessed. Our findings demonstrate that EPO reduces the relative area of abnormally high signal in PWI and DWI, increases cerebral blood volume, and decreases the number of apoptotic cells positive for TUNEL in the area injured by cerebral ischemia/reperfusion. The experiment pro- vides imaging evidence in vivo for EPO treating cerebral ischemia/reperfusion injury.

关键词： nerve regeneration nerve protection cerebral ischemia/reperfusion erythropoietin magnetic resonance imaging diffusion-weightedimaging apparent diffusion coefficient perfusion-weighted imaging cerebral blood volume mean transit time apoptosis neural regeneration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

erythropoietin inhibits ferroptosis and ameliorates neurological function after spinal cord injury

引用

Neural Regeneration Research 2023年第4期18卷 881-888页

作者： Yu Kang Rui Zhu Shuang Li Kun-Peng Qin Hao Tang Wen-Shan Shan Zong-Sheng Yin Department of Orthopedics The First Affiliated Hospital of Anhui Medical UniversityHefeiAnhui ProvinceChina

Ferroptosis is one of the critical pathological events in spinal cord *** has been reported to improve the recovery of spinal cord ***,whether ferroptosis is involved in the neuroprotective effects of erythropoietin on spinal cord injury has not been *** this study,we established rat models of spinal cord injury by modified Allen’s method and intraperitoneally administered 1000 and 5000 IU/kg erythropoietin once a week for 2 successive *** low and high doses of erythropoietin promoted recovery of hindlimb function,and the high dose of erythropoietin led to better *** dose of erythropoietin exhibited a stronger suppressive effect on ferroptosis relative to the low dose of *** effects of erythropoietin on inhibiting ferroptosis-related protein expression and restoring mitochondrial morphology were similar to those of Fer-1(a ferroptosis suppressor),and the effects of erythropoietin were largely diminished by RSL3(ferroptosis activator).In vitro experiments showed that erythropoietin inhibited RSL3-induced ferroptosis in PC12 cells and increased the expression of xCT and *** suggests that xCT and Gpx4 are involved in the neuroprotective effects of erythropoietin on spinal cord *** findings reveal the underlying anti-ferroptosis role of erythropoietin and provide a potential therapeutic strategy for treating spinal cord injury.

关键词： erythropoietin ferroptosis Gpx4 iron overload lipid peroxidation mechanism neurological function recovery spinal cord injury spinal neuron xCT

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

erythropoietin prevents PC12 cells from betaamyloid-induced apoptosis via PI3K⁄Akt pathway

引用

Translational Neurodegeneration 2012年第1期1卷 36-44页

作者： Sun Zhi-Kun Yang Hong-Qi Wang Zhi-Quan Pan Jing Hong Zhen Chen Sheng-Di Department of Neurology&Institute of Neurology Ruijin HospitalShanghai Jiaotong University School of MedicineShanghai 200025People’s Republic of China Lab of Neurodegenerative Diseases Institute of Health ScienceShanghai Institutes for Biological Sciences(SIBS)Chinese Academy of Sciences(CAS)&Shanghai Jiaotong University School of MedicineShanghai 200025People’s Republic of China Department of Neurology&Institute of Neurology Henan Provincial HospitalZhengzhou CityHenan province 450000People’s Republic of China

Background:Several studies indicated that erythropoietin(Epo)may provide remarkable neuroprotection in some neurological *** also showed the significant decrease of Epo immunoreactivity in the cerebral cortex and hippocampus in aged rats,suggesting the role of Epo in the pathogenesis of age-related neurodegenerative diseases such as ***:The protective effect of Epo was studied in differentiated PC12 cells treated with *** viability of the cells,the apoptosis of the cells and the level of Bax,Bcl-2,cleaved caspase-3 and cleaved PARP expression were detected by MTT,Hoechst 33258 staining and Western blotting ***:20μM Abeta(25-35)could induce a decreased viability and a increased apoptosis in PC12 cell in a timedependent ***,20μM Abeta(35-25)had no effect on cell viability and *** blot analysis also showed that Abeta(25-35)treatment could decrease the expression of Bcl-2(P<0.05)and increase the expression of Bax(P<0.05),Cleaved casapase-3(P<0.05),and Cleaved PARP(P<0.05).The pretreatment of Epo could effectively reverse all the above changes induced by Abeta_((25-35))(P<0.05).Furthermore,the protective effect of Epo could be blocked by PI3K inhibitor LY294002(P<0.05).Conclusions:Epo prevented cell injuries in PC12 cells exposed to the Abeta(25-35)and this effect may depend on the PI3K⁄Akt *** study provided an important evidence for the potential application of Epo in the therapy of Alzheimer’s disease.

关键词： Beta-amyloid peptide Apoptosis erythropoietin Alzheimer’s disease

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neuroprotective effects of erythropoietin on neurodegenerative and ischemic brain diseases: the role of erythropoietin receptor

引用

Neural Regeneration Research 2017年第9期12卷 1381-1389页

作者： Carolina Castillo Hernández Carlos Felipe Burgos Angela Hidalgo Gajardo Tiare Silva-Grecchi Javiera Gavilan Jorge Roberto Toledo Jorge Fuentealba Laboratory of Screening of Neuroactive Compounds Department of Physiology School of Biological Sciences University of Concepción Laboratory of Biotechnology and Biopharmaceutical Department of Pathophysiology School of Biological Sciences University of Concepción

erythropoietin （Epo） is a fundamental hormone in the regulation of hematopoiesis, and other secondary roles mediated by the binding of the hormone to its specific receptor （EpoR）, which leads to an activation of key signaling pathways that induce an increase in cell differentiation, apoptosis control and neuroprotection. It has been suggested that their ftmction depends on final conformation of glycosylations, related with affinity to the receptor and its half-life. The presence of EpoR has been reported in different tissues including central nervous system, where it has been demonstrated to exert a neuroprotective function against oxidative stress conditions, such as ischemic injury and neurodegenerative diseases. There is also evidence of an increase in EpoR expression in brain cell lysates of Alzheimer＇s patients with respect to healthy patients. These results are related with extensive in vitro experimental data of neuroprotection obtained from cell lines, primary cell cultures and hippocampal slices. Additionally, this data is correlated with in vivo experiments （water maze test） in mouse models of Alzheimer＇s disease where Epo treatment improved cognitive function. These stud- ies support the idea that receptor activation induces a neuroprotective effect in neurodegenerative disorders including dementias, and especially Alzheimer＇s disease. Taken together, available evidence suggests that Epo appears to be a central element for EpoR activation and neuroprotective properties in the central nervous system. In this review, we will describe the mechanisms associated with neuroprotection and its relation with the activation of EpoR in order with identify new targets to develop pharmacological strategies.

关键词： erythropoietin erythropoietin receptor neuroprotection anti-apoptosis Alzheimer's disease

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of erythropoietin on the expression of tumor necrosis factor-alpha and Bax after facial nerve axotomy in rats

引用

Neural Regeneration Research 2011年第6期6卷 444-449页

作者： Wei Zhang Shengyu Lue Ziying Yu Ming Bi Bin Sun Department of Oral and Maxillofacial Surgery School of Stomatology Jilin University Changchun 130041 Jilin Province China

This study sought to evaluate the effect of high-dose erythropoietin （EPO; 5 000 IU/kg） on the expression of tumor necrosis factor-alpha （TNF-α） and Bax in the facial nucleus after facial nerve transection in rats. A total of 42 Wistar rats of both genders were used in this study, and 40 rats were randomly divided into 2 groups： EPO group and model group. The EPO group was treated with EPO once a day for 5 days at a dose of 5 000 IU/kg body weight. The model group was treated with saline of the same amount. At day 3 after EPO （or saline） treatment, the right facial nerves of the 40 rats were transected at the level of the stylomastoid foramen, with the left sides untreated. The remaining 2 rats that did not undergo axotomy served as the control group. The surviving motor neurons in operated rats were counted in coronal paraffin sections of the facial nucleus. The expression of TNF-a and Bax in the facial nucleus was detected by immunohistochemical staining at days 3, 7, 14, 21, and 28 after axotomy. At days 14, 21, and 28 after facial nerve axotomy, a significantly greater proportion of facial motor neurons survived in the EPO group than in the model group. After axotomy, the expression of TNF-a and Bax increased in motor neurons in both the EPO and the model groups. TNF-o expression reached its peak level at day 14 after axotomy, while Bax expression reached its peak level at day 21. TNF-α expression was much lower in the EPO group than in the model group at all time points. No significant difference in Bax expression was found between the EPO and the model groups. These results indicate that high-dose EPO treatment attenuates the increase in TNF-α expression in the facial nucleus and reduces the loss of motor neurons after facial nerve transection in rats. However, high-dose EPO treatment has little effect on Bax expression.

关键词： erythropoietin tumor necrosis factor-a Bcl-2-associated X protein facial motor neuron

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Transplantation of erythropoietin gene-modified neural stem cells improves the repair of injured spinal cord

引用

Neural Regeneration Research 2015年第9期10卷 1483-1490页

作者： Min-fei Wu Shu-quan Zhang Rui Gu Jia-bei Liu Ye Li Qing-san Zhu Department of Orthopedics the Second Hospital of Jilin University Department of Orthopedics Tianjin Nankai Hospital Department of Orthopedics China-Japan Union Hospital of Jilin University

The protective effects of erythropoietin on spinal Here, the eukaryotic expression plasmid pcDNA3.1 cord injury have not been well described. human erythropoietin was transfected into rat neural stem cells cultured in vitro. A rat model of spinal cord injury was established using a free falling object. In the human erythropoietin-neural stem cells group, transfected neural stem cells were injected into the rat subarachnoid cavity, while the neural stem cells group was inject- ed with non-transfected neural stem cells. Dulbecco＇s modified Eagle＇s medium/F12 medium was injected into the rats in the spinal cord injury group as a control. At 1-4 weeks post injury, the motor function in the rat lower limbs was best in the human erythropoietin-neural stem ceils group, followed by the neural stem cells group, and lastly the spinal cord injury group. At 72 hours, compared with the spinal cord injury group, the apoptotic index and Caspase-3 gene and protein expressions were apparently decreased, and the bd-2 gene and protein expressions were noticeably increased, in the tissues surrounding the injured region in the human erythro- poietin-neural stem cells group. At 4 weeks, the somatosensory evoked potential latencies were cavities were clearly smaller and the motor and remarkably shorter in the human erythropoi- etin-neural stem cells group and neural stem cells group than those in the spinal cord injury group. These differences were particularly obvious in the human erythropoietin-neural stem cells group. More CM-Dil-positive cells and horseradish peroxidase-positive nerve fibers and larger amplitude motor and somatosensory evoked potentials were found in the human erythro- poietin-neural stem cells group and neural stem cells group than in the spinal cord injury group. Again, these differences were particularly obvious in the human erythropoietin-neural stem cells group. These data indicate that transplantation of erythropoietin gene-modified neural stem cells into the su

关键词： nerve regeneration spinal cord injury neural stem cells erythropoietin motor function subarachnoid cavity transplantation injury recovery neural regeneration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：