Erythropoietin upregulates growth associated protein-43 expression and promotes retinal ganglion cell axonal regeneration in vivo after optic nerve crush

作     者：Haibo Tan Xin Kang Yisheng Zhong Xi Shen Yu Cheng Qin Jiao Lianfu Deng 

作者机构：Department of Ophthalmology Ruijin Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200025 China Department of Clinical Pharmacology Changhai Hospital Affiliated to Second Mifitary Medical University Shanghai 200433 China Shanghai Institute of Traumatology and Orthopedics Shanghai 200025 China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2012年第7卷第4期

页      面：295-301页

核心收录：

学科分类：0905[农学-畜牧学] 08[工学] 09[农学] 0835[工学-软件工程] 081202[工学-计算机软件与理论] 0812[工学-计算机科学与技术（可授工学、理学学位）] 

基　　金：supported by the National Natural Science Foundation of China  No. 81070728 

主　　题：erythropoietin retinal ganglion cells axonal regeneration optic nerve crush neural regeneration 

摘      要：In this study, we established a rat model of optic nerve crush to explore the effects of erythropoietin on retinal ganglion cell axonal regeneration. At 15 days after injury in erythropoietin treated rats, retinal ganglion cell densities in regions corresponding to the 1/6, 3/6 and 5/6 ratios of the retinal radius were significantly increased. In addition, the number of growth associated protein-43 positive axons was significantly increased at different distances （50, 250 and 500 pm） from the crush site after erythropoietin treatment. Erythropoietin significantly increased growth associated protein-43 protein levels in the retina after crush injury, as determined by westem blot and immunofluorescence analysis. These results demonstrate that erythropoietin protects injured retinal ganglion cells and promotes axonal regeneration.