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Research on the developmental Venation of Calligraphy Seal Cutting Art Form from the Perspectives of Chinese Traditional Culture and Art Form

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International Journal of Technology Management 2016年第5期 40-43页

作者： Junhua Liu Hebei Academy Of Fine Arts ShijiazhuangHebei050700 China

In this paper, we conduct research on the developmental venation of the calligraphy seal cutting art form from the perspectives of Chinese traditional culture and art form. Seal cutting art of composition is a word or group of words arranged on the surface of the printing art, complex and changeable, is one of the most important part of the seal cutting art. Complete the creation of the group, must all have change, should show more creators on the composition of work force, therefore when designing should be repeated according to the text specifi c stroke, handwriting, the form and the relationship between word and word to design the structure of the right. Cultivating must practice the socialist core values and should be based on the basis of the Chinese excellent traditional culture, in carrying forward and greater China outstanding traditional culture in the process of makes it obtain nourishes the spirit and the value of the national identity. Our research provides the countermeasure for further modifi cation and optimization that is meaningful and necessary.

关键词： Art Form Traditional Culture developmental Venation Calligraphy Seal Cutting Art

Effects of β-mercaptoethanol Concentrations on developmental Ability of Oocytes in 6-week-old Dorper Sheep

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Agricultural Biotechnology 2019年第4期8卷 92-95页

作者： Yangyi MAO Huidi LUO Zhiwu WANG Peng ZHAO Guanwu ZHANG Jun LI Hongyu GUO Li ZHANG Lina XUE Wenqing DANG Institute of Animal Husbandry and Veterinary Shanxi Academy of Agricultural Sciences

This study was conducted to investigate the effects of different concentrations ofβ-mercaptoethanol on the development ability of oocytes in Dorper sheep at the age of 6 weeks.In this study,6 Dorper sheep of 6 weeks old were chosen to induce oocyte collection in vivo by gonadotropin,and A and B grade cumulus oocyte complexes were obtained(COCs)with different concentrations of(0,50,70 and 100μmol/L)ofβ-mercaptoethanol in the mature liquid.The cleavage rate and blastocyst rate were counted after matured oocytes and capacitated sperm were co-incubated and fertilized eggs were cultivated.The results showed that compared with the cleavage rate(59.13%)and the blastocyst rate(12.17%)of the control group,the cleavage rates(60.87%,63.48%and 65.22%)and blastocyst rates(14.78%,17.39%and 21.74%)of the test groups I,II and III were higher(P>0.05),and the order was test group III>test group II>test group I>control group.The cleavage rate and blastocyst rate of the 100μmol/Lβ-mercaptoethanol group increased by 6.09 percentage points and 9.57 percentage points,respectively.It indicated that the addition of a certain amount ofβ-mercaptoethanol in the mature liquid could improve the developmental ability of oocytes and the quality of fertilized eggs of the 6-week-old Doper sheep.When theβ-mercaptoethanol concentration was within 100μmol/L,the cleavage rate and the blastocyst rate of oocytes were on the rise.The optimal concentration ofβ-mercaptoethanol in the mature liquid still needs further study.

关键词： β-mercaptoethanol 6-week-old Dorper sheep Oocyte developmental ability

developmental programming of the metabolic syndrome critical windows for intervention

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World Journal of Diabetes 2011年第9期2卷 137-148页

作者： Mark H Vickers Liggins Institute and the National Research Centre for Growth and Development University of AucklandAuckland 1023New Zealand

Metabolic disease results from a complex interaction of many factors,including genetic,physiological,behavioral and environmental influences.The recent rate at which these diseases have increased suggests that environmental and behavioral influences,rather than genetic causes,are fuelling the present epidemic.In this context,the developmental origins of health and disease hypothesis has highlighted the link between the periconceptual,fetal and early infant phases of life and the subsequent development of adult obesity and the metabolic syndrome.Although the mechanisms are yet to be fully elucidated,this programming was generally considered an irreversible change in developmental trajectory.Recent work in animal models suggests that developmental programming of metabolic disorders is potentially reversible by nutritional or targeted therapeutic interventions during the period of developmental plasticity.This review will discuss critical windows of developmental plasticity and possible avenues to ameliorate the development of postnatal metabolic disorders following an adverse early life environment.

关键词： developmental programming Metabolic syndrome Obesity Type 2 diabetes Leptin Animal models Predictive adaptive responses

The short-chain perfluorinated compounds PFBS,PFHxS, PFBA and PFHxA, disrupt human mesenchymal stem cell self-renewal and adipogenic differentiation

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Journal of Environmental Sciences 2020年第2期32卷 187-199页

作者： Shuyu Liu Renjun Yang Nuoya Yin Francesco Faiola State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China College of Resources and Environment University of Chinese Academy of SciencesBeijing 100049China Wellcome Trust/CRUK Gurdon Institute Department of PathologyUniversity of CambridgeCambridge CB21QNUK

Per-and polyfluorinated alkyl substances(PFASs) are commonly used in industrial processes and daily life products.Because they are persistent, they accumulate in the environment, wildlife and humans.Although many studies have focused on two of the most representative PFASs, PFOS and PFOA, the potential toxicity of short-chain PFASs has not yet been given sufficient attention.We used a battery of assays to evaluate the toxicity of several four-carbon and six-carbon perfluorinated sulfonates and carboxyl acids(PFBS,PFHxS, PFBA and PFHxA), with a human mesenchymal stem cell(hMSC) system.Our results demonstrate significant cyto-and potential developmental toxicity for all the compounds analyzed, with shared but also distinct mechanisms of toxicity.Moreover, the effects of PFBS and PFHxS were stronger than those of PFBA and PFHxA, but occurred at higher doses compared to PFOS or PFOA.

关键词： Short-chain perfluorinated compounds Human mesenchymal stem cells(hMSCs) Adipogenesis/osteogenesis developmental toxicity PFBS/PFBA/PFHxS/PFHxA

Effects of mild intrauterine hypoperfusion in the second trimester on memory and learning function in rat offspring

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Neural Regeneration Research 2020年第11期15卷 2082-2088页

作者： Shao-Wei Yin Yuan Wang Yi-Lin Meng Cai-Xia Liu Department of Gynecology and Obstetrics Shengjing Hospital of China Medical UniversityShenyangLiaoning ProvinceChina Key Laboratory of Maternal-Fetal Medicine of Liaoning Province ShenyangLiaoning ProvinceChina Department of Anesthesiology Shengjing Hospital of China Medical UniversityShenyangLiaoning ProvinceChina

Mild intrauterine hypoperfusion(MIUH)is a serious pathological event that affects the growth and development of fetuses and offspring.MIUH can lead to growth restriction,low birth weight,neurodevelopmental disorders,and other adverse clinical outcomes.To study the effects of MIUH on learning and memory function in offspring,a model of MIUH was established by placing a coil(length 2.5 mm,diameter 0.24 mm)on the uterine artery and ovarian uterine artery of Sprague-Dawley rats in the second trimester of pregnancy(day 17).Next,120 mg/kg lithium chloride(the MIUH+Li group)or normal saline(the MIUH group)was injected intraperitoneally into these rats.In addition,120 mg/kg lithium chloride(the Li group)or normal saline(the SHAM group)was injected intraperitoneally into pregnant rats without coil placement.The Morris water maze was used to detect changes in learning and memory ability in the offspring at 4 weeks after birth.In the MIUH group,the escape latency and journey length before reaching the platform were both increased,and the number of times that the platform was crossed and the activity time in the target quadrant within 90 seconds were both decreased compared with the SHAM group.Immunofluorescence double staining and western blot assays demonstrated that hippocampal nestin and Ki67(both cell-proliferation-related proteins)expression was significantly downregulated in the MIUH group compared with the SHAM group.Furthermore,western blot assays were conducted to investigate changes in related signaling pathway proteins in the brains of offspring rats,and revealed that glycogen synthase kinase 3β(GSK3β)expression was upregulated andβ-catenin expression was downregulated in the MIUH group compared with the SHAM group.In addition,compared with the MIUH group,the expression levels of p-GSK3βandβ-catenin were upregulated in the MIUH+Li group.These results suggest that MIUH may affect learning and memory function in rat offspring by regulating the GSK3βsignaling pathway.The experimental procedures were approved by Anim

关键词： developmental neurobiology glycogen synthase kinase 3 intrauterine learning lithium memory offspring placenta diseases signal pathways β-catenin

ROS and oxidative burst:Roots in plant development

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Plant Diversity 2020年第1期42卷 33-43页

作者： Anuj Choudhary Antul Kumar Nirmaljit Kaur Department of Botany College of Basic Sciences and HumanitiesPunjab Agricultural UniversityLudhiana141004PunjabIndia

Reactive oxygen species(ROS)are widely generated in various redox reactions in plants.In earlier studies.ROS were considered toxic byproducts of aerobic metabolism.In recent years,it has become clear that ROS act as plant signaling molecules that participate in various processes such as growth and development.Several studies have elucidated the roles of ROS from seed germination to senescence.However,there is much to discover about the diverse roles of ROS as signaling molecules and their mechanisms of sensing and response.ROS may provide possible benefits to plant physiological processes by supporting cellular proliferation in cells that maintain basal levels prior to oxidative effects.Although ROS are largely perceived as either negative by-products of aerobic metabolism or makers for plant stress,elucidating the range of functions that ROS play in growth and development still require attention.

关键词： Oxidative signaling developmental processes Plant ROS Functional range

The Systems Biology of Lateral Root Formation: Connecting the Dots

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Molecular Plant 2019年第6期12卷 784-803页

作者： J.A.Santos Teixeira K.H.ten Tusscher Computational developmental Biology Group Department of Biology Utrecht University Utrecht the Netherlands

The root system is a major determinant of a plant's access to water and nutrients.The architecture of the root system to a large extent depends on the repeated formation of new lateral roots.In this review,we discuss lateral root development from a systems biology perspective.We focus on studies combining experiments with computational modeling that have advanced our understanding of how the auxin-centered regulatory modules involved in different stages of lateral root development exert their specific functions.Moreover,we discuss how these regulatory networks may enable robust transitions from one developmental stage to the next,a subject that thus far has received limited attention.In addition,we analyze how environmental factors impinge on these modules,and the different manners in which these environmental signals are being integrated to enable coordinated developmental decision making.Finally,we provide some suggestions for extending current models of lateral root development to incorporate multiple processes and stages.Only through more comprehensive models we can fully elucidate the cooperative effects of multiple processes on later root formation,and how one stage drives the transition to the next.

关键词： Arabidopsis lateral root developmental stages auxin signaling modules systems biology

Neonatal ketamine exposure-induced hippocampal neuroapoptosis in the developing brain impairs adult spatial learning ability

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Neural Regeneration Research 2020年第5期15卷 880-886页

作者： Dan Lyu Ning Tang Andrew W. Womack Yong-Jin He Qing Lin Department of Pain Management Tianjin First Center HospitalTianjinChina Department of Psychology College of ScienceUniversity of Texas at ArlingtonArlingtonTXUSA Reproductive Medicine Center the 960^th Hospital of the PLA Joint Logistics Support ForceJinanShandong ProvinceChina

Ketamine exposure can lead to selective neuroapoptosis in the developing brain.p66ShcA,the cellular adapter protein expressed selectively in immature neurons,is a known pro-apoptotic molecule that triggers neuroapoptosis when activated.Sprague-Dawley rats at postnatal day 7 were subcutaneously injected in the neck with ketamine 20 mg/kg,six times at 2-hour intervals.At 0,1,3,and 6 hours after final injection,western blot assay was used to detect the expression of cleaved caspase-3,p66ShcA,and phosphorylated p66ShcA.We found that the expression of activated p66ShcA and caspase-3 increased after ketamine exposure and peaked at 3 hours.The same procedure was performed on a different group of rats.At the age of 4 weeks,spatial learning and memory abilities were tested with the Morris water maze.Latency to find the hidden platform for these rats was longer than it was for control rats,although the residence time in the target quadrant was similar.These findings indicate that ketamine exposure resulted in p66ShcA being activated in the course of an apoptotic cascade during the neonatal period.This may have contributed to the deficit in spatial learning and memory that persisted into adulthood.The experimental protocol was approved by the Institutional Animal Care and Use Committee at the University of Texas at Arlington,USA (approval No.A13.008) on January 22,2013.

关键词： caspase-3 developmental neuroapoptosis hippocampus ketamine Morris water maze N-methyl-D-aspartate acid receptors p66ShcA spatial learning

BmHpo mutation induces smaller body size and late stage larval lethality in the silkworm,Bombyx mori

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Insect Science 2018年第6期25卷 1006-1016页

作者： Xia Xu Hong-Lun Bi Zhong-Jie Zhang Yang Yang Kai Li Yong-Ping Huang Yong Zhang Lin He School of Life Science East China Normal UniversityShanghaiChina Key Laboratory of Insect developmental and Evolutionary Biology Institute of Plant Physiology and EcologyShanghai Institutes for Biological SciencesChinese Academy of SciencesShanghaiChina

As a core member of the Hippo signaling pathway,Hpo plays a critical role in regulating growth and development.Previous studies reported that loss of function of Hpo results in increased proliferation,reduced apoptosis and induction of tissue overgrowth in Drosophila.In this study,we used CRISPR/Cas9(Clustered Regularly Interspaced Short Palindromic Repeats/Casg)to study Hpo gene (BmHpo)function in the lepidopteran insect Bombyx mori,known commonly as the silkworm.Sequence analysis of BmHpo revealed an array of deletions in mutants.We found that BmHpo knockout resulted in defects in body size regnlation,in developmental defects and pigment accumulation and early death. Our data show that BmHpo is essential for regulation of insect growth and development and that CRISPR/Cas9technology can serve as a basis for functional analysis of target genes in lepidopteran insects.

关键词： Bombyx mori CRISPR/Cas9 developmental defects Hippo signaling path- way Hpo