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The GATA transcription factor TaGATA1 recruits demethylase TaELF6-A1 and enhances seed dormancy in wheat by directly regulating TaABI5

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Journal of Integrative Plant Biology 2023年第5期65卷 1262-1276页

作者： Xuening Wei Yuyan Li Xiuliang Zhu Xin Liu Xingguo Ye Miaoping Zhou Zengyan Zhang The National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Biology and Genetic Improvement of Triticeae Crops Ministry of AgricultureInstitute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China JiangsuAcademyofAgricultural Sciences Nanjing210014China

Seed dormancy is an important agronomic trait in crops, and plants with low dormancy are prone to preharvest sprouting(PHS) under high-temperature and humid conditions. In this study,we report that the GATA transcription factor TaGATA1 is a positive regulator of seed dormancy by regulating TaABI5 expression in *** results demonstrate that TaGATA1 overexpression significantly enhances seed dormancy and increases resistance to PHS in wheat. Gene expression patterns, abscisic acid(ABA) response assay, and transcriptome analysis all indicate that TaGATA1 functions through the ABA signaling pathway. The transcript abundance of TaABI5, an essential regulator in the ABA signaling pathway,is significantly elevated in plants overexpressing TaGATA1. Chromatin immunoprecipitation assay(ChIP) and transient expression analysis showed that TaGATA1 binds to the GATA motifs at the promoter of TaABI5 and induces its *** also demonstrate that TaGATA1 physically interacts with the putative demethylase TaELF6-A1, the wheat orthologue of Arabidopsis ***–qPCR analysis showed that H3K27me3 levels significantly decline at the TaABI5 promoter in the TaGATA1-overexpression wheat line and that transient expression of TaELF6-A1 reduces methylation levels at the TaABI5 promoter, increasing TaABI5 expression. These findings reveal a new transcription module, including TaGATA1–TaELF6-A1–TaABI5, which contributes to seed dormancy through the ABA signaling pathway and epigenetic reprogramming at the target site. TaGATA1 could be a candidate gene for improving PHS resistance.

关键词： ABSCISIC ACID INSENSITIVE5(ABI5) common wheat demethylase GATA transcription factor preharvest sprouting seed dormancy

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The RNA N^(6)-methyladenosine demethylase ALKBH9B modulates ABA responses in Arabidopsis

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Journal of Integrative Plant Biology 2022年第12期64卷 2361-2373页

作者： Jun Tang Junbo Yang Qiang Lu Qian Tang Shuyan Chen Guifang Jia Synthetic and Functional Biomolecules Center Bejing National Laboratory for Molecular SciencesKey Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of EducationDepartment of Chemical BiologyCollege of Chemistry and Molecular EngineeringPeking UniversityBeijing 100871China Peking-Tsinghua Center for Life Sciences Beijing 100871China

The mRNA modification N^(6)-methyladenosine(m^(6)A)plays vital roles in plant development and biotic and abiotic stress *** RNA m^(6)A demethylase ALKBH9 B can remove m^(6)A in alfalfa mosaic virus RNA and plays roles in alfalfa mosaic virus infection in ***,it is unknown whether ALKBH9 B also exhibits demethylation activity and has a biological role in endogenous plant *** demonstrated here that mRNA m^(6)A modification is induced by the phytohormone abscisic acid(ABA)and that ALKBH9 B has m^(6)A demethylation activity on endogenous *** out ALKBH9 B led to hypersensitivity to ABA treatment during seed germination and early seedling *** further showed that ALKBH9 B removes the m^(6)A modification in the ABA INSENSITIVE 1(ABI1)and BRI1-EMS-SUPPRESSOR 1(BES1)transcripts following ABA treatment,affecting the stability of these ***,we determined that ALKBH9 B acts genetically upstream of the transcription factors ABI3 and ABI5,and its regulatory function in ABA responses depended on ABI3 and *** findings reveal the important roles of the m^(6)A modification in ABA responses and highlight the role of ALKBH9 Bmediated m^(6)A demethylation in regulating ABA responses post-transcriptionally.

关键词： ABA ALKBH9B Arabidopsis demethylase m^(6)A RNA modification

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Phylogenetic diversity of dimethylsulfoniopropionate-dependent demethylase gene dmdA in distantly related bacteria isolated from Arctic and Antarctic marine environments

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Acta Oceanologica Sinica 2019年第8期38卷 64-71页

作者： Yinxin Zeng Key Laboratory for Polar Science of State Oceanic Administration Polar Research Institute of China

Dimethylsulfoniopropionate(DMSP) is mainly produced by marine phytoplankton as an osmolyte, antioxidant,predator deterrent, or cryoprotectant. DMSP is also an important carbon and sulfur source for marine *** may metabolize DMSP via the demethylation pathway involving the DMSP demethylase gene(dmdA) or the cleavage pathway involving several different DMSP lyase genes. Most DMSP released into seawater is degraded by bacteria via demethylation. To test a hypothesis that the high gene frequency of dmdA among major marine taxa results in part from horizontal gene transfer(HGT) events, a total of thirty-one bacterial strains were isolated from Arctic Kongsfjorden seawater in this study. Analysis of 16S rRNA gene sequences showed that,except for strains BSw22118, BSw22131 and BSw22132 belonging to the genera Colwellia, Pseudomonas and Glaciecola, respectively, all bacteria fell into the genus Pseudoalteromonas. DmdA genes were detected in five distantly related bacterial strains, including four Arctic strains(Pseudoalteromonas sp. BSw22112, Colwellia ***22118, Pseudomonas sp. BSw22131 and Glaciecola sp. BSw22132) and one Antarctic strain(Roseicitreum antarcticum ZS2–28). Their dmdA genes showed significant similarities(97.7%–98.3%) to that of Ruegeria pomeroyi DSS–3, which was originally isolated from temperate coastal seawater. In addition, the sequence of the gene transfer agent(GTA) capsid protein gene(g5) detected in Antarctic strain ZS2–28 exhibited a genetically closely related to that of Ruegeria pomeroyi DSS–3. Among the five tested strains, only Pseudomonas sp. BSw22131 could grow using DMSP as the sole carbon source. The results of this study support the hypothesis of HGT for dmdA among taxonomically heterogeneous bacterioplankton, and suggest a wide distribution of functional gene(i.e., dmdA) in global marine environments.

关键词： dimethylsulfoniopropionate-dependent demethylase gene(dmdA) horizontal gene transfer marine bacteria Arctic Antarctic

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Development of the triazole-fused pyrimidine derivatives as highly potent and reversible inhibitors of histone lysine specific demethylase1(LSD1/KDM1A)

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Acta Pharmaceutica Sinica B 2019年第4期9卷 794-808页

作者： Zhonghua Li Lina Ding Zhongrui Li Zhizheng Wang Fengzhi Suo Dandan Shen Taoqian Zhao Xudong Sun Junwei Wang Ying Liu Liying Ma Bing Zhao Pengfei Geng Bin Yu Yichao Zheng Hongmin Liu School of Pharmaceutical Sciences Zhengzhou UniversityZhengzhou 450001China State Key Laboratory of Pharmaceutical Biotechnology Nanjing UniversityNanjing 210023China Co-Innovation Center of Henan Province for New Drug R&D and Preclinical Safety Zhengzhou 450001China Key Laboratory of Advanced Drug Preparation Technologies Ministry of Education of ChinaZhengzhou 450001China

Histone lysine specific demethylase 1(LSD1) has been recognized as an important modulator in post-translational process in epigenetics. Dysregulation of LSD1 has been implicated in the development of various cancers. Herein, we report the discovery of the hit compound 8 a(IC50=3.93 μmol/L) and further medicinal chemistry efforts, leading to the generation of compound 15 u(IC50=49 nmol/L, and Ki= 16 nmol/L), which inhibited LSD1 reversibly and competitively with H3 K4 me2, and was selective to LSD1 over MAO-A/B. Docking studies were performed to rationalize the potency ofcompound 15 u. Compound 15 u also showed strong antiproliferative activity against four leukemia cell lines(OCL-AML3, K562, THP-1 and U937) as well as the lymphoma cell line Raji with the IC50 values of 1.79, 1.30, 0.45, 1.22 and 1.40 μmol/L, respectively. In THP-1 cell line, 15 u significantly inhibited colony formation and caused remarkable morphological changes. Compound 15 u induced expression of CD86 and CD11 b in THP-1 cells, confirming its cellular activity and ability of inducing *** findings further indicate that targeting LSD1 is a promising strategy for AML treatment, the triazolefused pyrimidine derivatives are new scaffolds for the development of LSD1/KDM1 A inhibitors.

关键词： Epigenetic regulation Histone demethylase LSD1 Pyrimidine-triazole Mercapto heterocycles Antiproliferative ability AML treatment Structure–activity relationships(SARs)

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Crystallization and Preliminary Crystallographic Analysis of Histone demethylase PHF8

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生物物理学报 2009年第S1期25卷 320-321页

作者： Lin Yu, Yang Wang, Zhongzhou Chen State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China

The reversible process of histone methylation and demethylation plays important roles of epigenetic regulation1. For example, Truncating mutations in human PHF8 gene

关键词： Histone demethylase PHF8 Structure Protein Crystallography

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Genome-wide identification and expression analysis of DNA demethylase family in cotton

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Journal of Cotton Research 2019年第3期2卷 142-150页

作者： YANG Xiaomin LU Xuke CHEN Xiugui WANG Delong WANG Junjuan WANG Shuai GUO Lixue CHEN Chao WANG Xiaoge WANG Xinlei YE Wuwei Institute of Cotton Research of Chinese Academy of Agricultural Sciences/State Key Laboratory of Cotton Biology Key Laboratory for Cotton Genetic ImprovementMOAA

Background:DNA methylation is an important epigenetic factor that maintains and regulates gene *** mode and level of DNA methylation depend on the roles of DNA methyltransferase and demethylase,while DNA demethylase plays a key role in the process of DNA *** results showed that the plant’s DNA demethylase all contained conserved DNA glycosidase *** study identified the cotton DNA demethylase gene family and analyzed it using bioinformatics methods to lay the foundation for further study of cotton demethylase gene ***:This study used genomic information from diploid Gossypium raimondii JGI(D),Gossypium arboreum ***(A),Gossypium hirsutum ***(AD1) and Gossypium barbadebse L NAU(AD2) to Arabidopsis *** DNA demethylase genes sequence of Arabidopsis as reference,25 DNA demethylase genes were identified in cotton by BLAST *** are 4 genes in the genome D,5 genes in the genome A,10 genes in the genome AD1,and 6 genes in the genome *** gene structure and evolution were analyzed by bioinformatics,and the expression patterns of DNA demethylase gene family in Gossypium hirsutum L were *** the phylogenetic tree analysis,the DNA demethylase gene family of cotton can be divided into four subfamilies:REPRESSOR of SILENCING 1(ROS1),DEMETER(DME),DEMETER-LIKE 2(DML2),and DEMETER-LIKE3(DML3).The sequence similarity of DNA demethylase genes in the same species was higher,and the genetic relationship was also relatively *** of the gene structure revealed that the DNA demethylase gene family members of the four subfamilies varied *** them,the number of introns of ROS1 and DME subfamily was larger,and the gene structure was more *** the analysis of the conserved domain,it was known that the DNA demethylase family gene member has an endonuclease Ⅲ(END03 c) ***:The genes of the DNA demethylase family are distributed differently in different cotton species,and the ge

关键词： DNA demethylase Gossypium arboreum L. Gossypium barbadebse L. Gossypium hirsutum L. Gossypium raimondii

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Small-molecule targeting of oncogenic FTO demethylase in acute myeloid leukemia

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Science Foundation in China 2019年第2期27卷 45-45页

With the support of the National Natural Science Foundation of China and the Chinese Academy of Sciences,the laboratory led by Yang CaiGuang(杨财广)from Shanghai Institute of Materia Medica,Chinese Academy of Sciences,has identified a potential therapeutic small molecule for treating the acute myeloid leukemia(AML)by targeting the oncogenic RNA demethylase FTO,which was published in Figure Schematic diagram of small-molecular targeting of the oncogenic FTO *** Cell(2019,35:677—691).

关键词： FTO Small-molecule targeting of oncogenic FTO demethylase in acute myeloid leukemia AML

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Comparative Analysis of JmjC Domain-containing Proteins Reveals the Potential Histone demethylases in Arabidopsis and Rice

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Journal of Integrative Plant Biology 2008年第7期50卷 886-896页

作者： Falong Lu Guanglin Li Xia Cui Chunyan Liu Xiu-Jie Wang Xiaofeng Cao State Key Laboratory of Plant Genomics and National Center for Plant Gene Research Institute of Genetics and Developmental Biology the Chinese Academy of Sciences Beijing 100101 China Graduate University the Chinese Academy of Sciences Beijing 100039 China

Histone methylation homeostasis is achieved by controlling the balance between methylation and demethylation to maintain chromatin function and developmental regulation. In animals, a conserved Jumonji C （JmjC） domain was found in a large group of histone demethylases. However, it is still unclear whether plants also contain the JmjC domain- containing active histone demethylases. Here we performed genome-wide screen and phylogenetic analysis of JmjC domain-containing proteins in the dicot plant, Arabidopsis, and monocot plant rice, and found 21 and 20 JmjC domain-containing, respectively. We also examined the expression of JmjC domain-containing proteins and compared them to human JmjC counterparts for potential enzymatic activity. The spatial expression patterns of the Arabidopsis JmjC domain-containing genes revealed that they are all actively transcribed genes. These active plant JmjC domain-containing genes could possibly function in epigenetic regulation to antagonize the activity of the large number of putative SET domain-containing histone methyltransferase activity to dynamically regulate histone methylation homeostasis.

关键词： At JMJ demethylase demethylation histone Jumonji C methylation Os JMJ.

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Current Perspectives on Histone demethylases

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Acta Biochimica et Biophysica Sinica 2007年第2期39卷 81-88页

作者： Xiaoqing TIAN and Jingyuan FANG Shanghai Jiaotong University School of Medicine,Renji Hospital,shanghai Instituteof Digestive Disease,Shanghai 200001,China ShanghaiJiaotongUniversitySchoolofMedicine RenjiHospitalShanghaiInstituteofDigestiveDiseaseShanghai200001China

The posttranslational modification of histones plays an important role in chromatin *** methylation influences constitutive heterochromatin,genomic imprinting,X-chromosome inactivationand gene *** demethylase catalyzes the removal of methyl groups on lysine or arginineresidues of *** kinds of histone lysine demethylases have been identified, including lysine specificdemethylase 1 and Jumonji C (JmjC) domain family *** histone demethylases are involved in theregulation of gene *** modification is a dynamic process,and the imbalance of histonemethylation has been linked to ***,histone demethylases may represent a new target foranti-cancer therapy.

关键词： epigenetic modification histone methylation demethylase lysine specific demethylase 1 Jumonji C(JmjC)protein

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The long non-coding RNA DANA2 positively regulates drought tolerance by recruitingERF84 to promote JMJ29-mediated histone demethylation

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Molecular Plant 2023年第8期16卷 1339-1353页

作者： Pengxiang Zhang Reqing He Jun Yang Jingjing Cai Zhipeng Qu Rongxin Yang Jinbao Gu Zhen-Yu Wang David LAdelson Youlin Zhu Xiaofeng Cao Dong Wang Key Laboratory of Molecular Biology and Gene Engineering in Jiangxi Province College of Life ScienceNanchang UniversityJiangxi 330031China State Key Laboratory of Plant Genomics and National Center for Plant Gene Research Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100039China Department of Molecular and Biomedical Science School of Biological SciencesThe University of AdelaideAdelaideSA 5005Australia Institute of Nanfan&Seed Industry Guangdong Academy of SciencesGuangdong 510316China

Tens of thousands of long non-coding RNAs have been uncovered in plants,but few of them have been comprehensively studied for their biological function and molecular mechanism of their mode of ***,we show that the Arabidopsis long non-coding RNA DANA2 interacts with an AP2/ERF transcription factor ERF84 in the cell nucleus and then affects the transcription of JMJ29 that encodes a Jumonji C domain-containing histone H3K9 *** RNA sequencing(RNA-seq)and genetic analyses demonstrate that DANA2 positively regulates drought stress responses through ***29 positively regulates the expression of ERF15 and GOLS2 by modulation of H3K9me2 ***,mutation of JMJ29 causes decreased ERF15 and GOLS2 expression,resulting in impaired drought tolerance,in agreement with drought-sensitive phenotypes of dana2 and erf84 *** together,these results demonstrate that DANA2 is a positive regulator of drought response and works jointly with the transcriptional activator ERF84 to modulate JMJ29 expression in plant response to drought.

关键词： long non-coding RNA H3K9 demethylase transcription factor drought response

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