The RNA N^(6)-methyladenosine demethylase ALKBH9B modulates ABA responses in Arabidopsis

作     者：Jun Tang Junbo Yang Qiang Lu Qian Tang Shuyan Chen Guifang Jia Jun Tang;Junbo Yang;Qiang Lu;Qian Tang;Shuyan Chen;Guifang Jia

作者机构：Synthetic and Functional Biomolecules CenterBejing National Laboratory for Molecular SciencesKey Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of EducationDepartment of Chemical BiologyCollege of Chemistry and Molecular EngineeringPeking UniversityBeijing 100871China Peking-Tsinghua Center for Life SciencesBeijing 100871China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2022年第64卷第12期

页      面：2361-2373页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：the Postdoctoral Fellowship of Peking-Tsinghua Center for Life Sciences supported by the National Natural Science Foundation of China(nos.22225704,21820102008,92053109) the National Basic Research Program of China(2019YFA0802201) the Beijing Natural Science Foundation(Z200010) 

主　　题：ABA ALKBH9B Arabidopsis demethylase m^(6)A RNA modification 

摘      要：The mRNA modification N^(6)-methyladenosine(m^(6)A)plays vital roles in plant development and biotic and abiotic stress *** RNA m^(6)A demethylase ALKBH9 B can remove m^(6)A in alfalfa mosaic virus RNA and plays roles in alfalfa mosaic virus infection in ***,it is unknown whether ALKBH9 B also exhibits demethylation activity and has a biological role in endogenous plant *** demonstrated here that mRNA m^(6)A modification is induced by the phytohormone abscisic acid(ABA)and that ALKBH9 B has m^(6)A demethylation activity on endogenous *** out ALKBH9 B led to hypersensitivity to ABA treatment during seed germination and early seedling *** further showed that ALKBH9 B removes the m^(6)A modification in the ABA INSENSITIVE 1(ABI1)and BRI1-EMS-SUPPRESSOR 1(BES1)transcripts following ABA treatment,affecting the stability of these ***,we determined that ALKBH9 B acts genetically upstream of the transcription factors ABI3 and ABI5,and its regulatory function in ABA responses depended on ABI3 and *** findings reveal the important roles of the m^(6)A modification in ABA responses and highlight the role of ALKBH9 Bmediated m^(6)A demethylation in regulating ABA responses post-transcriptionally.