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Journal of Integrative Agriculture 2021年第5期20卷 1314-1326页

作者： CHEN Chang-long YUAN Fang LI Xiao-ying MA Rong-cai XIE Hua Beijing Agro-Biotechnology Research Center Beijing Academy of Agriculture and Forestry SciencesBeijing 100097P.R.China Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology Beijing 100097P.R.China

Chinese cabbage(Brassica rapa ***)suffers from soft rot disease caused by Pectobacterium carotovorum(Pc).To uncover the mechanisms underlying the defense response of Chinese cabbage to Pc,we constructed a suppression subtractive hybridization(SSH)library from Pc-infected cabbage and obtained 1919 non-redundant expressed sequence tags(ESTs),which were used for cDNA *** detected 800 differentially expressed genes(DEGs)in cabbage at different time points post-Pc inoculation,which were further confirmed by quantitative real-time *** quarter of these DEGs were involved in the biotic stress pathways visualized by *** them,8,8,1,3,and 2 DEGs were related to jasmonic acid(JA),ethylene(ET),JA+ET,auxin,and abscisic acid(ABA)signaling pathways,respectively,while no DEG was detected for salicylic acid(SA)*** of phytohormone production in the Pc-infected leaves showed that JA and ET production was increased,while SA production was *** with JA,methyl jasmonate(MeJA),the ET precursor 1-aminocyclopropane-1-carboxylate(ACC),or combinations thereof,reduced the disease severity,and the JA and JA+ACC treatments were superior and performed equally *** findings suggest that JA and ET may act synergistically against Pc infection in Chinese cabbage,and JA-mediated signaling might be the most significant.

关键词： Brassica rapa Pectobacterium carotovorum gene expression defense response hormone signaling

ZmDRR206 functions in maintaining cell wall integrity during maize seedling growth and defense response to external stresses

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The Crop Journal 2023年第6期11卷 1649-1664页

作者： Tao Zhong Suining Deng Mang Zhu Xingming Fan Mingliang Xu Jianrong Ye National Maize Improvement Center/Center for Crop Functional Genomics and Molecular Breeding China Agricultural UniversityBeijing 100193China Institute Food Crops Yunnan Academy of Agricultural SciencesKunming 650205YunnanChina

Plants adaptively change their cell wall composition and structure during their growth,development,and interactions with environmental *** proteins(DIRs)contribute to environmental adaptations by dynamically reorganizing the cell wall and/or by generating defense compounds.A maize DIR,ZmDRR206,was previously reported to play a dominant role in regulation of storage nutrient accumulation in endosperm during maize kernel *** we show that ZmDRR206 mediates maize seedling growth and disease resistance by coordinately regulating biosynthesis of cell wall components for cell-wall integrity(CWI)*** of ZmDRR206 was induced in maize seedlings upon pathogen ***206 overexpression in maize resulted in reduced seedling growth and photosynthetic activity but increased disease resistance and drought tolerance,revealing a tradeoff between growth and ***,ZmDRR206 overexpression reduced the contents of primary metabolites and down-regulated genes involved in photosynthesis,while increasing the contents of major cell wall components,defense phytohormones,and defense metabolites,and up-regulated genes involved in defense and cell-wall biosynthesis in ***206-overexpressing seedlings were resistant to cell-wall stress imposed by isoxaben,and ZmDRR206 physically interacted with ZmCesA10,which is a cellulose synthase *** findings suggest a mechanism by which ZmDRR206 coordinately regulates biosynthesis of cell-wall components for CWI maintenance during maize seedling growth,and might be exploited for breeding strong disease resistance in maize.

关键词： Cell-wall integrity Cell-wall biosynthesis Dirigent protein ZmDRR206 defense response Seedling growth

Lignin metabolism involves Botrytis cinerea BcGsl-induced defense response in tomato

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Lignin metabolism involves Botrytis cinerea BcGsl-induced de...

iTRAQ-based quantitative proteomics analysis of defense responses triggered by the pathogen Rhizoctonia solani infection in rice

中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者： Yang Chenyu Liang Yingbo Qiu Dewen Zeng Hongmei Yuan Jingjing Yang Xiufen Key Laboratory of Control of Biological Hazard Factors(Plant Origin) for Agri-product Quality and Safety Ministry of AgricultureInstitute of Plant Protection

BcGs1,a cell wall degration enzyme(CWDE) is originally derived from Botrytis *** previous study revealed BcGs1 could trigger defense response and protect plants against various *** this study,two domans of Glyco-hydro 15(GH15) and CBM20lucoamylase(CBM20) of BcGs1 were transiently expressed in Nicotiana benthamiana and the results revealed that the two domains were required for BcGs1 full necrosis *** best understand the defense response mechanism underlying this BcGs1 elicitation in tomato,the differential protein expression profiles between BcGs1 and TrisHCl buffer treatment plants was performed using the iTRAQ-based quantitative proteome *** 71 up-regulated with ratio >1.3 and 38 down-regulated proteins with ratio defense response in ***,the experimental evidence showed BcGsl triggered ROS burst,increased level of phenylalanine-ammonia lyase(PAL) and peroxidase(POD)enzyme activity and lignin ***,histochemical analysis revealed that BcGsl infiltration tomato leaves exhibited cell wall thickness compared with untreated ***,BcGs1 activated basal defense response and lignin metabolism contributed to BcGs1-induced resistance to *** infection in tomato.

关键词： Fungal protein elicitor defense response Phenylpropanoid Lignin Botrytis cinerea

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Journal of Integrative Agriculture 2022年第1期21卷 139-152页

作者： FENG Zhi-ming GAO Peng ZHAO Jian-hua WANG Guang-da ZHANG Hui-min CAO Wen-lei XUE Xiang ZHANG Ya-fang MA Yu-yin HUA Rong CHEN Zong-xiang CHEN Xi-jun HU Ke-ming ZUO Shi-min Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding Agricultural CollegeYangzhou UniversityYangzhou 225009P.R.China Co-Innovation Center for Modern Production Technology of Grain Crops of Jiangsu Province/Key Laboratory of Crop Genetics and Physiology of Jiangsu Province Yangzhou UniversityYangzhou 225009P.R.China Joint International Research Laboratory of Agriculture and Agri-Product Safety Ministry of Education of China/Institutes of Agricultural Science and Technology DevelopmentYangzhou UniversityYangzhou 225009P.R.China Yangzhou Polytechnic College Yangzhou 225000P.R.China Jiangsu Hongqi Seed Stock Co. Ltd.Taizhou 225311P.R.China

The soil-borne necrotrophic fungus Rhizoctonia solani is one of destructive fungi causing severe yield losses in various important crops. However, the host defense mechanisms against the invasion of this pathogen are poorly understood. In this study, we employed an i TRAQ-based quantitative proteomic approach to investigate host proteins responsive to R. solani using the resistant rice cultivar YSBR1. As a whole, we identified 319 differentially accumulated proteins(DAPs) after inoculation of rice plants with R. solani. Functional categorization analysis indicates that these DAPs cover a broad range of functions. Notably, a substantial portion of the DAPs are involved in cell redox homeostasis, carbohydrate metabolism, and phenylpropanoid biosynthesis, or belong to pathogenesis-related proteins, indicating that these processes/proteins play important roles in host defense against R. solani. Interestingly, all of the DAPs involved in photosynthesis and chlorophyll biosynthetic processes, and part of the DAPs involved in phenylpropanoid biosynthesis, show reduced accumulation after R. solani infection, suggesting that R. solani probably inhibits host photosynthetic system and phenylpropanoid biosynthesis to facilitate infection and colonization. In conclusion, our results provide both valuable resources and new insights into the molecular mechanisms underlying rice and R. solani interaction.

关键词： rice sheath blight Rhizoctonia solani proteomic defense response

PlaD： A Transcriptomics Database for Plant defense responses to Pathogens, Providing New Insights into Plant Immune System

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Genomics, Proteomics & Bioinformatics 2018年第4期16卷 283-293页

作者： Huan Qi Zhenhong Jiang Kang Zhang Shiping Yang Fei He Ziding Zhang State Key Laboratory of Agrobiotechnology College of Biological Sciences China Agricultural UniversityBeijing 100193China Jiangxi Key Laboratory of Molecular Medicine The Second Affiliated Hospital of Nanchang UniversityNanchang 330006China Department of Plant Pathology and the Ministry of Agriculture Key Laboratory for Plant Pathology China Agricultural UniversityBeijing 100193China Biology Department Brookhaven National LabUptonNY 11967USA

High-throughput transcriptomics technologies have been widely used to study plant transcriptional reprogramming during the process of plant defense responses, and a large quantity of gene expression data have been accumulated in public repositories. However, utilization of these data is often hampered by the lack of standard metadata annotation. In this study, we curated2444 public pathogenesis-related gene expression samples from the model plant Arabidopsis and three major crops （maize, rice, and wheat）. We organized the data into a user-friendly database termed as PlaD. Currently, PlaD contains three key features. First, it provides large-scale curated data related to plant defense responses, including gene expression and gene functional annotation ***, it provides the visualization of condition-specific expression profiles. Third, it allows users to search co-regulated genes under the infections of various pathogens. Using PlaD, we conducted a large-scale transcriptome analysis to explore the global landscape of gene expression in the curated data. We found that only a small fraction of genes were differentially expressed under multiple conditions, which might be explained by their tendency of having more network connections and shorter network distances in gene networks. Collectively, we hope that PlaD can serve as an important and comprehensive knowledgebase to the community of plant sciences, providing insightful clues to better understand the molecular mechanisms underlying plant immune responses. PlaD is freely available at http：//***/plad/*** or http：//***/plad/***.

关键词： PlaD Plant defense response Transcriptomics databaseGene expression analysis

Host Active defense responses Occur within 24 Hours after Pathogen Inoculation in the Rice Blast System

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Rice science 2007年第4期14卷 302-310页

作者： WANG Zhong-hua JIA Yu-lin LIN Hui Adair INTERN Barbara VALENT J. Neil RUTGER Institute of Biotechnology Zhejiang Wanli University Ningbo 315100 China USDA-ARS Dale Bumpers National Rice Research Center P. O. Box 1090 Stuttgart AR 72160 USA Department of Plant Pathology University of Arkansas Fayetteville AR 72701 USA Department of Plant Pathology Kansas State University 4024 Throckmorton Plant Science Center Manhattan KS 66506-5502 USA

Phenotypical, cytological and molecular responses of rice to the fungus Magnaporthe grisea were studied using rice cultivars and lesion mimic plants. The cultivar Katy was susceptible to several virulent M. grisea isolates, and a Sekiguchi like-lesion mimic mutant of Katy （LmmKaty） showed enhanced resistance to these isolates. Lesion mimic phenotype of LmmKaty was rapidly induced by virulent M. grisea isolates or by avirulent ones only at high levels of inoculum. Autofluorescence （a sign of an active defense response） was visible under ultraviolet light 24 h after localized inoculation in the incompatible interaction, whereas, not evident in the compatible interaction. Autofluorescence was also observed in LmmKaty 20 h after pathogen inoculation, indicating that rapid cell death is a mechanism of LmmKaty to restrict pathogen invasion. Rapid accumulations of defense related （DR） gene transcripts, phenylalanine ammonia lyase and β-glucanase, were observed beginning at 6 h and were obvious at 16 h and 24 h after inoculation in an incompatible interaction. Rapid transcript accumulations of PR-1 and chitinase had occurred by 24 h after inoculation in an incompatible interaction. Accumulations of these transcripts were delayed in the compatible interaction. These results indicate that host active defense responses occur 24 h after pathogen inoculation and that LmmKaty exhibits enhanced resistance to M. grisea. It is suggested that the autofluorescence and expression of the DR genes after heavy inoculation are important cytological and molecular markers respectively for early determination of the host response to M. grisea in the rice blast system.

关键词： rice (Oryza sativa) lesion mimic mutant disease resistance cytology defense response Magnaporthe grisea

GhWRKY33 negatively regulates jasmonate-mediated plant defense to Verticillium dahliae

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Plant Diversity 2023年第3期45卷 337-346页

作者： Yunrui Ji Minghui Mou Huimin Zhang Ruling Wang Songguo Wu Yifen Jing Haiyan Zhang Lanxin Li Zhifang Li Ligang Chen CAS Key Laboratory of Tropical Plant Resources and Sustainable Use Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesMenglunMenglaYunnan 666303China Center of Economic Botany Core Botanical GardensChinese Academy of SciencesMenglunMenglaYunnan 666303China University of Chinese Academy of Sciences Beijing 100049China School of Life Sciences Henan UniversityKaifeng 475004China

Verticillium wilt,caused by Verticillium dahliae,seriously restricts the yield and quality improvement of *** studies have revealed the involvement of WRKY members in plant defense against ***,but the underlying mechanisms involved need to be further ***,we demonstrated that Gossypium hirsutum WRKY DNA-binding protein 33(GhWRKY33) functions as a negative regulator in plant defense against ***33 expression is induced rapidly by *** and methyl jasmonate,and overexpression of GhWRKY33 reduces plant tolerance to *** in *** RT-PCR analysis revealed that expression of several JA-associated genes was significantly repressed in GhWRKY33 overexpressing transgenic *** one-hybrid analysis revealed that GhWRKY33 may repress the transcription of both AtERF1 and GhERF2 through its binding to their ***-protein interaction analysis suggested that GhWRKY33 interacts with *** JASMONATE ZIM-domain protein 3(GhJAZ3).Similarly,overexpression of GhJAZ3 also decreases plant tolerance to ***,GhJAZ3 acts synergistically with GhWRKY33 to suppress both AtERF1 and GhERF2 *** results imply that GhWRKY33 may negatively regulate plant tolerance to *** via the JA-mediated signaling pathway.

关键词： GhWRKY33 Verticillium dahliae Jasmonate acid GhJAZ3 defense response

A signaling cascade mediating fruit trait development via phosphorylation-modulated nuclear accumulation of JAZ repressor

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Journal of Integrative Plant Biology 2024年第6期66卷 1106-1125页

作者： Wei Wang Jinyao Ouyang Yating Li Changsheng Zhai Bing He Huahan Si Kunsong Chen Jocelyn K.C.Rose Wensuo Jia College of Horticulture China Agricultural UniversityBeijing 100193China College of Agriculture and Biotechnology Zhejiang UniversityZijingang CampusHangzhou 310058China Plant Biology Section School of Integrative Plant ScienceCornell UniversityIthaca14853NYUSA Institute of Horticulture Crops Xinjiang Academy of Agricultural SciencesUrumqi 830000China

It is generally accepted that jasmonate-ZIM domain(JAZ)repressors act to mediate jasmonate(JA)signaling via CORONATINE-INSENSITIVE1(COI1)-mediated ***,we report a cryptic signaling cascade where a JAZ repressor,FvJAZ12,mediates multiple signaling inputs via phosphorylation-modulated subcellular translocation rather than the COI1-mediated degradation mechanism in strawberry(Fragaria vesca).FvJAZ12 acts to regulate flavor metabolism and defense response,and was found to be the target of Fv MPK6,a mitogen-activated protein kinase that is capable of responding to multiple signal ***6 phosphorylates FvJAZ12 at the amino acid residues S179 and T183 adjacent to the PY residues,thereby attenuating its nuclear accumulation and relieving its repression for FvMYC2,which acts to control the expression of lipoxygenase 3(FvLOX3),an important gene involved in JA biosynthesis and a diverse array of cellular *** data reveal a previously unreported mechanism for JA signaling and decipher a signaling cascade that links multiple signaling inputs with fruit trait development.

关键词： defense response JAZ repressor phosphorylation strawberry subcellular translocation

Nuclear Trafficking During Plant Innate Immunity

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Molecular Plant 2008年第3期1卷 411-422页

作者： Jun Liu Gitta Coaker Department of Plant Pathology The University of California Davis CA USA

Land plants possess innate immune systems that can control resistance against pathogen infection. Conceptually, there are two branches of the plant innate immune system. One branch recognizes conserved features of microbial pathogens, while a second branch specifically detects the presence of pathogen effector proteins by plant resistance （R） genes. Innate immunity controlled by plant R genes is called effector-triggered immunity. Although R genes can recognize all classes of plant pathogens, the majority can be grouped into one large family, encoding proteins with a nucleotide binding site and C-terminal leucine rich repeat domains. Despite the importance and number of R genes present in plants, we are just beginning to decipher the signaling events required to initiate defense responses. Recent exciting discoveries have implicated dynamic nuclear trafficking of plant R proteins to achieve effector-triggered immunity. Furthermore, there are several additional lines of evidence implicating nucleo-cyctoplasmic trafficking in plant disease resistance, as mutations in nucleoporins and importins can compromise resistance signaling. Taken together, these data illustrate the importance of nuclear trafficking in the manifestation of disease resistance mediated by R genes.

关键词： DISEASE-RESISTANCE GENES POWDERY MILDEW FUNGUS POTATO-VIRUS-X IMPORTIN-ALPHA defense response EXPORT RECEPTOR PROTEIN-KINASES

Identification of differentially expressed proteins in poplar leaves in-duced by Marssonina brunnea f. sp. Multigermtubi

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Journal of Genetics and Genomics 2008年第1期35卷 49-60页

作者： Kun Yuan Bo Zhang Yanmei Zhang Qiang Cheng Mingxiu Wang Minren Huang Key Laboratory of Forest Genetics and Gene Engineering Nanjing Forestry University Nanjing 210037 China Rubber Research Institute Chinese Academy of Tropical Agricultural Sciences Danzhou 571737 China South Subtropical Crops Research Institute Chinese Academy of Tropical Agricultural Sciences Zhanjiang 524091 China

Black spot disease in poplar is a disease of the leaf caused by fungus. The major pathogen is Marssonina brunnea f. sp. multigermtubi. To date, little is known about the molecular mechanism of poplar （M. brunnea） interaction. In order to identify the proteins related to disease resistance and understand its molecular basis, the clone ＂NL895＂（P. euramericana CL＂NL895＂）, which is highly resistant to M. brunnea f. sp. multigermtubi, was used in this study. We used two-dimensional gel electrophoresis （2-DE） and mass spectrometry （MS） to identify the proteins in poplar leaves that were differentially expressed in response to black spot disease pathogen, M. brunnea f. sp. multigermtubi. Proteins extracted from poplar leaves at 0, 12, 24, 48, and 72 h after pathogen-inoculation were separated by 2-DE, About 500 reproducible protein spots were detected, of which 40 protein spots displayed differential expression in levels and were subjected to Matrix assisted laser desorption/ionization time of flight mass spectrometry （MALDI-TOF MS） followed by database searching. According to the function, the identified proteins were sorted into five categories, that is, protein synthesis, metabolism, defense response and unclassified proteins.

关键词： black spot disease defense response two-dimensional gel electrophoresis (2-DE) matrix assisted laser desorption/ionization time of flightmass spectrometry (MALDI-TOF MS) poplar