Host Active Defense Responses Occur within 24 Hours after Pathogen Inoculation in the Rice Blast System
Host Active Defense Responses Occur within 24 Hours after Pathogen Inoculation in the Rice Blast System作者机构：Institute of Biotechnology Zhejiang Wanli University Ningbo 315100 China USDA-ARS Dale Bumpers National Rice Research Center P. O. Box 1090 Stuttgart AR 72160 USA Department of Plant Pathology University of Arkansas Fayetteville AR 72701 USA Department of Plant Pathology Kansas State University 4024 Throckmorton Plant Science Center Manhattan KS 66506-5502 USA
出 版 物：《Rice science》 (水稻科学（英文版）)
年 卷 期：2007年第14卷第4期
页 面：302-310页
基 金：Special Foundation of Education Bureau in Zhejiang Province, (20050218) Arkansas Rice Research and Promotion Board
主 题：rice (Oryza sativa) lesion mimic mutant disease resistance cytology defense response Magnaporthe grisea
摘 要：Phenotypical, cytological and molecular responses of rice to the fungus Magnaporthe grisea were studied using rice cultivars and lesion mimic plants. The cultivar Katy was susceptible to several virulent M. grisea isolates, and a Sekiguchi like-lesion mimic mutant of Katy （LmmKaty） showed enhanced resistance to these isolates. Lesion mimic phenotype of LmmKaty was rapidly induced by virulent M. grisea isolates or by avirulent ones only at high levels of inoculum. Autofluorescence （a sign of an active defense response） was visible under ultraviolet light 24 h after localized inoculation in the incompatible interaction, whereas, not evident in the compatible interaction. Autofluorescence was also observed in LmmKaty 20 h after pathogen inoculation, indicating that rapid cell death is a mechanism of LmmKaty to restrict pathogen invasion. Rapid accumulations of defense related （DR） gene transcripts, phenylalanine ammonia lyase and β-glucanase, were observed beginning at 6 h and were obvious at 16 h and 24 h after inoculation in an incompatible interaction. Rapid transcript accumulations of PR-1 and chitinase had occurred by 24 h after inoculation in an incompatible interaction. Accumulations of these transcripts were delayed in the compatible interaction. These results indicate that host active defense responses occur 24 h after pathogen inoculation and that LmmKaty exhibits enhanced resistance to M. grisea. It is suggested that the autofluorescence and expression of the DR genes after heavy inoculation are important cytological and molecular markers respectively for early determination of the host response to M. grisea in the rice blast system.
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