Identification of differentially expressed proteins in poplar leaves in-duced by Marssonina brunnea f. sp. Multigermtubi

作     者：Kun Yuan Bo Zhang Yanmei Zhang Qiang Cheng Mingxiu Wang Minren Huang 

作者机构：Key Laboratory of Forest Genetics and Gene Engineering Nanjing Forestry University Nanjing 210037 China Rubber Research Institute Chinese Academy of Tropical Agricultural Sciences Danzhou 571737 China South Subtropical Crops Research Institute Chinese Academy of Tropical Agricultural Sciences Zhanjiang 524091 China 

出 版 物：《Journal of Genetics and Genomics》 (遗传学报（英文版）)

年 卷 期：2008年第35卷第1期

页      面：49-60页

核心收录：

学科分类：0907[农学-林学] 08[工学] 0829[工学-林业工程] 09[农学] 

基　　金：This work was supported by the National Natural Science Foundation of China (No. 30230300) 

主　　题：black spot disease defense response two-dimensional gel electrophoresis (2-DE) matrix assisted laser desorption/ionization time of flightmass spectrometry (MALDI-TOF MS) poplar 

摘      要：Black spot disease in poplar is a disease of the leaf caused by fungus. The major pathogen is Marssonina brunnea f. sp. multigermtubi. To date, little is known about the molecular mechanism of poplar （M. brunnea） interaction. In order to identify the proteins related to disease resistance and understand its molecular basis, the clone ＂NL895＂ （P. euramericana CL＂NL895＂）, which is highly resistant to M. brunnea f. sp. multigermtubi, was used in this study. We used two-dimensional gel electrophoresis （2-DE） and mass spectrometry （MS） to identify the proteins in poplar leaves that were differentially expressed in response to black spot disease pathogen, M. brunnea f. sp. multigermtubi. Proteins extracted from poplar leaves at 0, 12, 24, 48, and 72 h after pathogen-inoculation were separated by 2-DE, About 500 reproducible protein spots were detected, of which 40 protein spots displayed differential expression in levels and were subjected to Matrix assisted laser desorption/ionization time of flight mass spectrometry （MALDI-TOF MS） followed by database searching. According to the function, the identified proteins were sorted into five categories, that is, protein synthesis, metabolism, defense response and unclassified proteins.