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The DUF579 proteins GhIRX15s regulate cotton fiber development by interacting with proteins involved in xylan synthesis

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The Crop Journal 2024年第4期12卷 1112-1125页

作者： Mengyun Li Feng Chen Jingwen Luo Yanan Gao Jinglong Cai Wei Zeng Monika S.Doblin Gengqing Huang Wenliang Xu Hubei Key Laboratory of Genetic Regulation and Integrative Biology School of Life SciencesCentral China Normal UniversityWuhan 430079HubeiChina Zhejiang Provincial Key Laboratory of Plant Evolutionary Ecology and Conservation School of Life SciencesTaizhou UniversityTaizhou 318000ZhejiangChina La Trobe Institute for Sustainable Agriculture&Food School of AgricultureBiomedicine and EnvironmentLa Trobe UniversityBundooraVIC 3086Australia

cotton provides the most abundant natural fiber for the textile *** mature cotton fiber largely consists of secondary cell walls with the highest proportion of cellulose and a small amount of hemicellulose and *** dissect the roles of hemicellulosic polysaccharides during fiber development,four IRREGULAR XYLEM 15(IRX15)genes,GhIRX15-1/-2/-3/-4,were functionally characterized in *** genes encode DUF579 domain-containing proteins,which are homologs of AtIRX15 involved in xylan *** four GhIRX15 genes were predominantly expressed during fiber secondary wall thickening,and the encoded proteins were localized to the Golgi *** GhIRX15 gene could restore the xylan deficient phenotype in the Arabidopsis irx15irx15l double *** of GhIRX15s in cotton resulted in shorter mature fibers with a thinner cell wall and reduced cellulose content as compared to the wild ***,GhIRX15-2 and GhIRX15-4 formed homodimers and *** addition,the GhIRX15s showed physical interaction with glycosyltransferases GhGT43C,GhGT47A and GhGT47B,which are responsible for synthesis of the xylan backbone and reducing end ***,the GhIRX15s can form heterocomplexes with enzymes involved in xylan modification and side chain synthesis,such as GhGUX1/2,GhGXM1/2 and *** findings suggest that GhIRX15s participate in fiber xylan biosynthesis and modulate fiber development via forming large multiprotein complexes.

关键词： cotton fiber Xylan biosynthesis GhIRX15s Protein-protein interaction Protein complexes

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Phytosterol content and the campesterol:sitosterol ratio influence cotton fiber development: role of phytosterols in cell elongation

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Science China(Life Sciences) 2016年第2期59卷 183-193页

作者： Shasha Deng Ting Wei Kunling Tan Mingyu Hu Fang Li Yunlan Zhai Shue Ye Yuehua Xiao Lei Hou Yan Pei Ming Luo Key Laboratory of Biotechnology and Crop Quality Improvement Ministry of AgricultureBiotechnology Research Center Southwest University

Phytosterols play an important role in plant growth and development, including cell division, cell elongation, embryogenesis, cellulose biosynthesis, and cell wall formation. cotton fiber, which undergoes synchronous cell elongation and a large amount of cellulose synthesis, is an ideal model for the study of plant cell elongation and cell wall biogenesis. The role of phytosterols in fiber growth was investigated by treating the fibers with tfidemorph, a sterol biosynthetic inhibitor. The inhibition of phy- tosterol biosynthesis resulted in an apparent suppression of fiber elongation in vitro or in planta. The determination of phy- tosterol quantity indicated that sitosterol and campesterol were the major phytosterols in cotton fibers; moreover, higher con- centrations of these phytosterols were observed during the period of rapid elongation of fibers. Furthermore, the decrease and increase in campesterol：sitosterol ratio was associated with the increase and decease in speed of elongation, respectively, dur- ing the elongation stage. The increase in the ratio was associated with the transition from cell elongation to secondary cell wall synthesis. In addition, a number of phytosterol biosynthetic genes were down-regulated in the short fibers of ligon lintless-1 mutant, compared to its near-isogenic wild-type TM-1. These results demonstrated that phytosterols play a crucial role in cot- ton fiber development, and particularly in fiber elongation.

关键词： cotton fiber phytosterols gene expression tridemorph ligon lintless-1

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Analysis of Five Differentially Expressed Gene Families in Fast Elongating cotton fiber

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Acta Biochimica et Biophysica Sinica 2004年第1期36卷 51-56页

作者： Jian-Xun FENG, Sheng-Jian JI, Yong-Hui SHI, Yu XU, Gang WEI, and Yu-Xian ZHU( National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China ) National Laboratory of Protein Engineering and Plant Genetic Engineering College of Life Sciences Peking University Beijing 100871 China

Using the suppression subtractive hybridization method, we isolated five gene families, including proline-rich proteins (PRPs), arabinogalactan proteins (AGPs), expansins, tubulins and lipid transfer proteins (LTPs), from fast elongating cotton fiber cells. Expression profile analysis using cDNA array technology showed that most of these gene families were highly expressed during early cotton fiber developmental stages (0-20 days post anthesis, DPA). Many transcripts accumulated over 50-fold in 10 DPA fiber cells than in 0 DPA samples. The entire gene family -AGP, together with 20 individual members in other 4 gene families, are reported in cotton for the first time. Accumulation of cell wall proteins, wall loosening enzymes, microtubules and lipid transfer proteins may contribute directly to the elongation and development of fiber cells.

关键词： cotton fiber cDNA array cell elongation arabinogalactan protein lipid transfer protein

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Gene expression and metabolite profiles of cotton fiber during cell elongation and secondary cell wall synthesis

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Cell Research 2007年第5期17卷 422-434页

作者： Jin-Ying Gou1,2,3 Ling-Jian Wang1,2 Shuang-Ping Chen4 Wen-Li Hu1,2 Xiao-Ya Chen1,2 1 National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China 2 CAS Center of Shanghai Chenshan Botanical Garden, 300 Fenglin Road, Shanghai 200032, China 3 Graduate School of Chinese Academy of Sciences, Shanghai 200032, China 4 Department of Computer Science and Technology, University of Science and Technology of China, Hefei 230026, China National Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China CAS Center of Shanghai Chenshan Botanical Garden Shanghai China Graduate School of Chinese Academy of Sciences Shanghai China Department of Computer Science and Technology University of Science and Technology of China Hefei China

cotton fibers elongate rapidly after initiation of elongation, eventually leading to the deposit of a large amount ofcellulose. To reveal features of cotton fiber cells at the fast elongation and the secondary cell wall synthesis stages, wecompared the respective transcriptomes and metabolite profiles. Comparative analysis of transcriptomes by cDNA arrayidentified 633 genes that were differentially regulated during fiber development. Principal component analysis (PCA)using expressed genes as variables divided fiber samples into four groups, which are diagnostic of developmental *** grouping results are also found if we use non-polar or polar metabolites as variables for PCA of developingfibers. Auxin signaling, wall-loosening and lipid metabolism are highly active during fiber elongation, whereas cellulosebiosynthesis is predominant and many other metabolic pathways are downregulated at the secondary cell wall synthesisstage. Transcript and metabolite profiles and enzyme activities are consistent in demonstrating a specialization process ofcotton fiber development toward cellulose synthesis. These data demonstrate that cotton fiber cell at a certain stage has itsown unique feature, and developmental stages of cotton fiber cells can be distinguished by their transcript and metaboliteprofiles. During the secondary cell wall synthesis stage, metabolic pathways are streamed into cellulose synthesis.

关键词： cotton fiber transcriptome metabolite profile auxin cell elongation cellulose synthesis

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The miR319-Targeted GhTCP4 Promotes the Transition from Cell Elongation to Wall Thickening in cotton fiber

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Molecular Plant 2020年第7期13卷 1063-1077页

作者： Jun-Feng Cao Bo Zhao Chao-Chen Huang Zhi-Wen Chen Ting Zhao Hong-Ru Liu Guan-Jing Hu Xiao-Xia Shangguan Chun-Min Shan- Ling-Jian Wang Tian-Zhen Zhang Jonathan F.Wendel Xue-Ying Guan Xiao-Ya Chen National Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology/CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200032China Plant Stress Biology Center Institute of Plant Physiology and Ecology/CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200032China University of Chinese Academy of Sciences Shanghai 200032China School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China Agronomy Department College of Agriculture and BiotechnologyZhejiang UniversityHangzhouZhejiang 310058China Department of Ecology Evolution and Organismal BiologyIowa State UniversityAmesIA 50011.USA Plant Science Research Center Shanghai Key Laboratory of Plant Functional Genomics and ResourcesShanghai Chenshan Botanical GardenShanghai 201602China

Plant cell growth involves a complex interplay among cell-wall expansion, biosynthesis, and, in specific tissues, secondary cell wall (SCW) deposition, yet the coordination of these processes remains elusive. cotton fiber cells are developmentally synchronous, highly elongated, and contain nearly pure cellulose when mature. Here, we report that the transcription factor GhTCP4 plays an important role in balancing cotton fiber cell elongation and wall synthesis. During fiber development the expression of miR319 declines while GhTCP4 transcript levels increase, with high levels of the latter promoting SCW deposition. GhTCP4 interacts with a homeobox-containing factor, GhHOX3, and repressing its transcriptional activity. GhTCP4 and GhHOX3 function antagonistically to regulate cell elongation, thereby establishing temporal control of fiber cell transition to the SCW stage. We found that overexpression of GhTCP4A upregulated and accelerated activation of the SCW biosynthetic pathway in fiber cells, as revealed by transcriptome and promoter activity analyses, resulting in shorter fibers with varied lengths and thicker walls. In contrast, GhTCP4 downregulation led to slightly longer fibers and thinner cell walls. The GhHOX3–GhTCP4 complex may represent a general mechanism of cellular development in plants since both are conserved factors in many species, thus providing us a potential molecular tool for the design of fiber traits.

关键词： cell elongation secondary cell wall cotton fiber Gossypium hirsutum TCP microRNA

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Effects of Increased Night Temperature on Cellulose Synthesis and the Activity of Sucrose Metabolism Enzymes in cotton fiber

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Journal of Integrative Agriculture 2013年第6期12卷 979-988页

作者： TIAN Jing-shan HU Yuan-yuan GAN Xiu-xia ZHANG Ya-li HU Xiao-bing GOU Ling LUO Hong-hai ZHANG Wang-feng Key Laboratory of Oasis Eco-Agriculture Xinjiang Production and Construction Group/College of Agronomy Shihezi University

Temperature is one of the key factors that influence cotton fiber synthesis at the late growth stage of cotton. In this paper, using two early-maturing cotton varieties as experimental materials, night temperature increase was stimulated in the field using far-infrared quartz tubes set in semi-mobile incubators and compared with the normal night temperatures （control） in order to investigate the effects of night temperature on the cotton fiber cellulose synthesis during secondary wall thickening. The results showed that the activity of sucrose synthase （SuSy） and sucrose phosphate synthase （SPS） quickly increased and remained constant during the development of cotton fiber, while the activity of acid invertase （AI） and alkaline invertase （NI） decreased, increased night temperatures prompted the rapid transformation of sugar, and all the available sucrose fully converted into cellulose. With night temperature increasing treatment, an increase in SuSy activity and concentration of sucrose indicate more sucrose converted into UDPG （uridin diphosphate-glucose） during the early and late stages of cotton fiber development. Furthermore, SPS activity and the increased concentration of fructose accelerated fructose degradation and reduced the inhibition of fructose to SuSy; maintaining higher value of allocation proportion of invertase and sucrose during the early development stages of cotton fiber, which was propitious to supply a greater carbon source and energy for cellulose synthesis. Therefore, the minimum temperature in the nightime was a major factor correlated with the activity of sucrose metabolism enzymes in cotton fiber. Consequently, soluble sugar transformation and cellulose accumulation were closely associated with the minimum night temperature.

关键词： cotton fiber night temperature sucrose metabolism enzyme activity

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Molecular Biological and Biochemical Studies Reveal New Pathways Important for cotton fiber Development

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Journal of Integrative Plant Biology 2007年第1期49卷 69-74页

作者： Yu Xu Hong-Bin Li Yu-Xian Zhu National Laboratory of Protein Engineering and Plant Genetic Engineering Peking University Beijing 100871 China

As one of the longest single-celled seed trichomes, fibers provide an excellent model for studying fundamental biological processes such as cell differentiation, cell expansion, and cell wall biosynthesis. In this review, we summarize recent progress in cotton functional genomic studies that characterize the dynamic changes in the transcriptomes of fiber cells. Extensive expression profilings of cotton fiber transcriptomes have provided comprehensive information, as quite a number of transcription factors and enzyme-coding genes have been shown to express preferentially during the fiber elongation period. Biosynthesis of the plant hormone ethylene is found significantly upregulated during the fiber growth period as revealed by both microarray analysis and by biochemical and physiological studies. It is suggested that genetic engineering of the ethylene pathway may improve the quality and the productivity of cotton lint. Many metabolic pathways, such as biosynthesis of cellulose and matrix polysaccharides are preferentially expressed in actively growing fiber cells. Five gene families, including proline-rich proteins （PRP）, arabinogalactan proteins （AGP）, expansins, tubulins and lipid transfer proteins （LTP） are activated during early fiber development, indicating that they may also be needed for cell elongation. In conclusion, we identify a few areas of future research for cotton functional genomic studies.

关键词： cotton fiber functional genomics plant hormone transcriptome transcription factors

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Boosting Seed Development as a New Strategy to Increase cotton fiber Yield and Quality

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Journal of Integrative Plant Biology 2013年第7期55卷 572-575页

作者： Yong-Ling Ruan Department of Biology School of Environmental and Life SciencesThe University of Newcastle Australia Australia-China Research Centre for Crop ImprovementThe University of Newcastle

cotton （Gossypium spp.） is the most important textile crop worldwide due to its cellulosic mature fibers, which are single-celled hairs initiated from the cotton ovule epidermis at anthesis. Research to improve cotton fiber yield and quality in recent years has been largely focused on identifying genes regulating fiber cell initiation, elonga- tion and cellulose synthesis. However, manipulating some of those candidate genes has yielded no effect or only a marginally positive effect on fiber yield or quality. On the other hand, evolutionary comparison and transgenic studies have clearly shown that cotton fiber growth is intimately controlled by seed development. Therefore,I propose that enhancing seed development could be a more effective and achievable strategy to increase fiber yield and quality.

关键词： Auxin cotton fiber seed development sugars seed maternal and filial tissues.

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Molecular studies of cellulose synthase supercomplex from cotton fiber reveal its unique biochemical properties

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Science China(Life Sciences) 2022年第9期65卷 1776-1793页

作者： Xingpeng Wen Yufeng Zhai Li Zhang Yanjun Chen Zhiyuan Zhu Gang Chen Kun Wang Yuxian Zhu Institute for Advanced Studies Wuhan UniversityWuhan 430072China Hubei Hongshan Laboratory Wuhan 430070China College of Life Sciences Wuhan UniversityWuhan 430072China School of Physical Science and Technology ShanghaiTech UniversityShanghai 201210China

cotton fiber is a highly elongated and thickened single cell that produces large quantities of cellulose,which is synthesized and assembled into cell wall microfibrils by the cellulose synthase complex(CSC).In this study,we report that in cotton(Gossypium hirsutum)fibers harvested during secondary cell wall(SCW)synthesis,GhCesA 4,7,and 8 assembled into heteromers in a previously uncharacterized 36-mer-like cellulose synthase supercomplex(CSS).This super CSC was observed in samples prepared using cotton fiber cells harvested during the SCW synthesis period but not from cotton stem tissue or any samples obtained from ***-out of any of GhCesA 4,7,and 8 resulted in the disappearance of the CSS and the production of fiber cells with no SCW *** fiber CSS showed significantly higher enzyme activity than samples prepared from knock-out cotton *** found that the microfibrils from the SCW of wild-type cotton fibers may contain 72 glucan chains in a bundle,unlike other plant materials ***4,7,and 8 restored both the dwarf and reduced vascular bundle phenotypes of their orthologous Arabidopsis mutants,potentially by reforming the CSC *** complementation was not observed when non-orthologous CesA genes were used,indicating that each of the three subunits is indispensable for CSC formation and for full cellulose synthase *** of cotton CSS will increase our understanding of the regulation of SCW biosynthesis.

关键词： cotton fiber cellulose synthase supercomplex(CSS) GhCesA 36-mer-like SCW 72 chain model microfibril

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Transcriptional regulation of phospholipid transport in cotton fiber elongation by GhMYB30D04–GhHD1 interaction complex

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Journal of Integrative Plant Biology 2024年第11期66卷 2431-2449页

作者： Qingwei Song Chuanhui Du Yiyang Xu Jin Wang Min Lin Kaijing Zuo Single Cell Research Center College of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China College of Agriculture Henan UniversityKaifeng 450046China

cotton fiber length is basically determined by wellcoordinated gene expression and phosphatidylinositol phosphates(PIPs) accumulation during fiber elongation but the regulatory mechanism governing PIPs transport remains ***,we report a MYB transcription factor GhMYB30D04 in Gossypium hirsutum that promotes fiber elongation through modulating the expression of PIP transporter gene *** of GhMYB30D04gene in cotton(KO) results in a reduction of GhLTPG1 transcripts with lower accumulation of PIPs,leading to shorter fibers and lower fiber ***,GhMYB30D04 overexpression(GhMYB30D04-OE) causes richer PIPs and longer cotton fibers,mimicking the effects of exogenously applying PIPs on the ovules of GhMYB30D04-KO and wild ***,GhMYB30D04 interacts with GhHD1,the crucial transcription factor of fiber initiation,to form an activation complex stabilized by PIPs,both of which upregulate GhLTPG1 *** omics-analysis revealed that higher and extended expressions of LTPG1 in fiber elongation mainly correlate with the variations of the GhMYB30D04 gene between two cotton allotetraploids,contributing to longer fiber in *** work clarifies a mechanism by which GhHD1–GhMYB30D04 form a regulatory module of fiber elongation to tightly control PIP *** work still has an implication that GhMYB30D04–GhHD1 associates with development transition from fiber initiation to elongation.

关键词： cotton fiber fiber cell elongation MYB–HD-ZIP transcription complex phospholipid transport transcriptional regulation

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