Gene expression and metabolite profiles of cotton fiber during cell elongation and secondary cell wall synthesis

作     者：Jin-Ying Gou1,2,3 Ling-Jian Wang1,2 Shuang-Ping Chen4 Wen-Li Hu1,2 Xiao-Ya Chen1,2 1 National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China 2 CAS Center of Shanghai Chenshan Botanical Garden, 300 Fenglin Road, Shanghai 200032, China 3 Graduate School of Chinese Academy of Sciences, Shanghai 200032, China 4 Department of Computer Science and Technology, University of Science and Technology of China, Hefei 230026, China 

作者机构：National Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China CAS Center of Shanghai Chenshan Botanical Garden Shanghai China Graduate School of Chinese Academy of Sciences Shanghai China Department of Computer Science and Technology University of Science and Technology of China Hefei China 

出 版 物：《Cell Research》 (细胞研究(英文版))

年 卷 期：2007年第17卷第5期

页      面：422-434页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：国家863计划 国家973计划 国家植物转基因与产业化专项 

主　　题：cotton fiber transcriptome metabolite profile auxin cell elongation cellulose synthesis 

摘      要：Cotton fibers elongate rapidly after initiation of elongation, eventually leading to the deposit of a large amount ofcellulose. To reveal features of cotton fiber cells at the fast elongation and the secondary cell wall synthesis stages, wecompared the respective transcriptomes and metabolite profiles. Comparative analysis of transcriptomes by cDNA arrayidentified 633 genes that were differentially regulated during fiber development. Principal component analysis (PCA)using expressed genes as variables divided fiber samples into four groups, which are diagnostic of developmental *** grouping results are also found if we use non-polar or polar metabolites as variables for PCA of developingfibers. Auxin signaling, wall-loosening and lipid metabolism are highly active during fiber elongation, whereas cellulosebiosynthesis is predominant and many other metabolic pathways are downregulated at the secondary cell wall synthesisstage. Transcript and metabolite profiles and enzyme activities are consistent in demonstrating a specialization process ofcotton fiber development toward cellulose synthesis. These data demonstrate that cotton fiber cell at a certain stage has itsown unique feature, and developmental stages of cotton fiber cells can be distinguished by their transcript and metaboliteprofiles. During the secondary cell wall synthesis stage, metabolic pathways are streamed into cellulose synthesis.