The DUF579 proteins GhIRX15s regulate cotton fiber development by interacting with proteins involved in xylan synthesis

作     者：Mengyun Li Feng Chen Jingwen Luo Yanan Gao Jinglong Cai Wei Zeng Monika S.Doblin Gengqing Huang Wenliang Xu Mengyun Li;Feng Chen;Jingwen Luo;Yanan Gao;Jinglong Cai;Wei Zeng;Monika S.Doblin;Gengqing Huang;Wenliang Xu

作者机构：Hubei Key Laboratory of Genetic Regulation and Integrative BiologySchool of Life SciencesCentral China Normal UniversityWuhan 430079HubeiChina Zhejiang Provincial Key Laboratory of Plant Evolutionary Ecology and ConservationSchool of Life SciencesTaizhou UniversityTaizhou 318000ZhejiangChina La Trobe Institute for Sustainable Agriculture&FoodSchool of AgricultureBiomedicine and EnvironmentLa Trobe UniversityBundooraVIC 3086Australia 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2024年第12卷第4期

页      面：1112-1125页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0901[农学-作物学] 

基　　金：supported by the National Natural Science Foundation of China(31970516 and 32372104) the Foundation of Hubei Hongshan Laboratory(2021hszd014) 

主　　题：Cotton fiber Xylan biosynthesis GhIRX15s Protein-protein interaction Protein complexes 

摘      要：Cotton provides the most abundant natural fiber for the textile *** mature cotton fiber largely consists of secondary cell walls with the highest proportion of cellulose and a small amount of hemicellulose and *** dissect the roles of hemicellulosic polysaccharides during fiber development,four IRREGULAR XYLEM 15(IRX15)genes,GhIRX15-1/-2/-3/-4,were functionally characterized in *** genes encode DUF579 domain-containing proteins,which are homologs of AtIRX15 involved in xylan *** four GhIRX15 genes were predominantly expressed during fiber secondary wall thickening,and the encoded proteins were localized to the Golgi *** GhIRX15 gene could restore the xylan deficient phenotype in the Arabidopsis irx15irx15l double *** of GhIRX15s in cotton resulted in shorter mature fibers with a thinner cell wall and reduced cellulose content as compared to the wild ***,GhIRX15-2 and GhIRX15-4 formed homodimers and *** addition,the GhIRX15s showed physical interaction with glycosyltransferases GhGT43C,GhGT47A and GhGT47B,which are responsible for synthesis of the xylan backbone and reducing end ***,the GhIRX15s can form heterocomplexes with enzymes involved in xylan modification and side chain synthesis,such as GhGUX1/2,GhGXM1/2 and *** findings suggest that GhIRX15s participate in fiber xylan biosynthesis and modulate fiber development via forming large multiprotein complexes.