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Regenerative Biomaterials 2021年第2期8卷 43-49页

作者： Shijie Qiu Lijin Liang Peng Zou Qi Chen Department of Otorhinolaryngology-Head and Neck Surgery The First Affiliated Hospital of Zhejiang University79 Qingchun RoadHangzhou310003People’s Republic of China Department of Otorhinolaryngology-Head and Neck Surgery Lihuili Hospital of Ningbo University57 Xingning RoadNingbo315041People’s Republic of China Ningbo Regen Biotech Co. Ltd199 East Hexiao RoadNingbo315157People’s Republic of China

There has been an increase in the incidence of hypopharyngeal and cervical esophageal cancer worldwide,and hence growing needs for hypopharyngeal and cervical esophageal tissue *** work produced a bi-layer composite scaffold with decellularized small intestine submucosa and polylactic-co-glycolic acid,which resembled the layered architectures of its intended *** decellularized small intestine submucosa contained minimal residual DNA(52.5±61.2 ng/mg)and the composite scaffold exhibited satisfactory mechanical properties(a tensile modulus of 21.1±64.8 MPa,an ultimate tensile strength of 14.0±62.9MPa and a failure strain of 26.9±65.1%).The interactions between cells and the respective layers of the scaffold were characterized by CCK-8 assays,immunostaining and Western *** cell proliferation and phenotypic behaviors were *** results have provided an important basis for the next-step in vivo studies of the scaffold,and bode well for its future clinical applications.

关键词： small intestine submucosa polylactic-co-glycolic acid hypopharyngeal and cervical esophageal cancer tissue repair composite scaffold

Osteogenic and antibacterial dual functions of a novel levofloxacin loaded mesoporous silica microspheres/nano-hydroxyapatite/ polyurethane composite scaffold

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Genes & Diseases 2021年第2期8卷 193-202页

作者： Zhiping Kuang Guangming Dai Ruijie Wan Dongli Zhang Chen Zhao Cheng Chen Jidong Li Hongchen Gu Wei Huang Department of Orthopedic Surgery The First Affiliated Hospital of Chongqing Medical UniversityChongqing400016PR China Department of Orthopaedic Surgery Chongqing Traditional Chinese Medicine HospitalChongqing400011PR China Research Center for Nano-Biomaterials Analytical&Testing CenterSichuan UniversityChengduSichuan Province610065PR China State Key Laboratory of Oncogenes and Related Genes School of Biomedical EngineeringMed-X Research InstituteShanghai Jiaotong UniversityShanghai200240PR China

Lev/MSNs/n-HA/PU has been proved to be a novel scaffold material to treat bone defect caused by chronic *** have previously identified that this material can effectively treat chronic osteomyelitis caused by Staphylococcus aureus in ***,the potential mechanisms of antibacterial and osteogenic induction properties remain ***,for osteogenesis property,immunohistochemistry,PCR,and Western blot were performed to detect the expression of osteogenic ***,flow cytometry and TUNEL were applied to analyze MC3T3-E1 proliferation and *** antibacterial property,the material was co-cultivated with bacteria,bacterial colony forming units was counted and the release time of the effective levofloxacin was assayed by agar disc-diffusion ***,scanning electron microscope was applied to observe adhesion of *** terms of osteogenic induction,we found BMSCs adherently grew more prominently on Lev/MSNs/n-HA/***/MSNs/n-HA/PU also enhanced the expression of osteogenic markers including OCN and COL1a1,as well as effectively promoted the transition from G1 phase to G2 ***,Lev/MSNs/n-HA/PU could reduce apoptosis of ***,both Lev/MSNs/n-HA/PU and n-HA/PU materials could inhibit bacterial colonies,while Lev/MSNs/n-HA/PU possessed a stronger antibacterial activities,and lower bacterial adhesion than n-HA/*** results illustrated that Lev/MSNs/n-HA/PU composite scaffold possess favorable compatibility in vitro,which induce osteogenic differentiation of MSCs,promote proliferation and differentiation of MC3T3-E1,and inhibit ***,clear in vitro antibacterial effect of Lev/MSNs/n-HA/PU was also *** summary,this study replenishes the potential of Lev/MSNs/n-HA/PU composite scaffold possess dual functions of anti-infection and enhanced osteogenesis for future clinical application.

关键词： Antibacterial composite scaffold Levofloxacin Nanobiomaterial Osteogenesis

Dexamethasone-Loaded PLGA Microspheres Incorporated PLLA/PLGA/PCL composite scaffold for Bone Tissue Engineering

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Journal of Donghua University(English Edition) 2017年第1期34卷 159-163页

作者：苗莹珂聂伟王伟忠周小军何创龙 College of Chemistry Chemical Engineering and Biotechnology Donghua University Shanghai 201620 China

The combination of micro-carriers and polymer scaffolds as promising bone grafts have attracted considerable interest in recent *** poly(L-lactic acid)/poly(lactic-co-glycolic acid)/polycaprolactone(PLLA/PLGA/PCL)composite scaffold with porous structure was fabricated by thermally induced phase separation(TIPS).Dexamethasone(DEX)was incorporated into PLGA microspheres and then loaded on the PLLA/PLGA/PCL *** physicochemical properties of the prepared composite scaffold were *** morphology of rat bone marrow mesenchymal stem cells(BMSCs)grown on scaffolds was observed using scanning electron microscope(SEM)and fluorescence *** resultsshowedthatthePLLA/PLGA/PCLscaffoldhad interconnected macropores and biomimetic nanofibrous *** addition,DEX can be released from scaffold in a sustained *** importantly,DEX loaded composite scaffold can effectively support the proliferation of BMSCs as indicated by fluorescence observation and cell proliferation *** results suggested that the prepared PLLA/PLGA/PCL composite scaffold incorporating drug-loaded PLGA microspheres could hold great potential for bone tissue engineering applications.

关键词： composite scaffold poly(lactic-co-glycolic acid)(PLG A) microsphere dexamethasone bone tissue engineering

3D-printed GelMA/CaSiO_(3)composite hydrogel scaffold for vascularized adipose tissue restoration

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Regenerative Biomaterials 2023年第1期10卷 1073-1084页

作者： Jupei Zhang Zhen Zeng Yanxin Chen Li Deng Yanxin Zhang Yumei Que Yiren Jiao Jiang Chang Zhihong Dong Chen Yang School of Mechanical Engineering Chengdu UniversityChengdu 610106China Joint Centre of Translational Medicine The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325000China Wenzhou Institute University of Chinese Academy of SciencesWenzhou 325000China College of Materials Science and Opto-electronic Technology University of Chinese Academy of SciencesBeijing 100049China Shanghai Institute of Ceramics Chinese Academy of SciencesShanghai 200050China

The increased number of mastectomies,combined with rising patient expectations for cosmetic and psychosocial outcomes,has necessitated the use of adipose tissue restoration ***,the therapeutic effect of current clinical strategies is not satisfying due to the high demand of personalized customization and the timely vascularization in the process of adipose ***,a composite hydrogel scaffold was prepared by three-dimensional(3D)printing technology,applying gelatin methacrylate anhydride(GelMA)as printing ink and calcium silicate(CS)bioceramic as an active ingredient for breast adipose tissue *** in vitro experiments showed that the composite hydrogel scaffolds could not only be customized with controllable architectures,but also significantly stimulated both 3T3-L1 preadipocytes and human umbilical vein endothelial cells in multiple cell behaviors,including cell adhesion,proliferation,migration and ***,the composite scaffold promoted vascularized adipose tissue restoration under the skin of nude mice in *** findings suggest that 3D-printed GelMA/CS composite scaffolds might be a good candidate for adipose tissue engineering.

关键词： 3D-printing bioceramic composite scaffold adipogenesis angiogenesis

Personalized composite scaffolds for accelerated cell-and growth factor-free craniofacial bone regeneration

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Bioactive Materials 2024年第11期41卷 427-439页

作者： Mirae Kim Xinlong Wang Yiming Li Zitong Lin Caralyn P.Collins Yugang Liu Yujin Ahn Hsiu-Ming Tsal Joseph W.Song Chongwen Duan Yi Zhu Cheng Sun Tong-Chuan He Yuan Luo Russell R.Reid Guillermo A.Ameer Department of Biomedical Engineering Northwestern UniversityEvanstonIL60208USA Center for Advanced Regenerative Engineering Northwestern UniversityEvanstonIL60208USA Department of Preventive Medicine Feinberg School of MedicineNorthwestern UniversityChicagoIL60611USA Department of Mechanical Engineering Northwestern UniversityEvanstonIL60208 USA Department of Chemical and Biomolecular Engineering University of Illinois at Urbana-ChampaignUrbanaIL61820USA Department of Radiology The University of ChicagoChicagoIL60637USA Molecular Oncology Laboratory Department of Orthopedic Surgery and Rehabilitation MedicineThe University of Chicago Medical CenterChicagoIL60637USA Clinical and Translational Sciences Institute Northwestern UniversityChicagoIL60611USA Center for Collaborative AI in Healthcare Institute for AI in MedicineNorthwestern UniversityChicagoIL60611USA Laboratory of Craniofacial Biology and Development Section of Plastic and Reconstructive SurgeryDepartment of SurgeryThe University of Chicago Medical CenterChicagoIL60637USA Department of Surgery Feinberg School of MedicineNorthwestern UniversityChicagoIL60611USA Chemistry of Life Process Institute Northwestern UniversityChicagoIL60208USA International Institute for Nanotechnology Northwestern UniversityEvanstonIL60208USA

Approaches to regenerating bone often rely on integrating biomaterials and biological signals in the form of cells or ***,from a translational point of view,these approaches are challenging due to the sourcing and quality of the biologic,unpredictable immune responses,complex regulatory paths,and high *** describe a simple manufacturing process and a material-centric 3D-printed composite scaffold system(CSS)that offers distinct advantages for clinical *** CSS comprises a 3D-printed porous polydiolcitrate-hydroxyapatite composite elastomer infused with a polydiolcitrate-graphene oxide hydrogel *** a micro-continuous liquid interface production 3D printer,we fabricate a precise porous ceramic scaffold with 60 wt%hydroxyapatite resembling natural *** resulting scaffold integrates with a thermoresponsive hydrogel composite in situ to fit the defect,which is expected to enhance surface contact with surrounding tissue and facilitate *** antioxidative properties of citrate polymers prevent long-term inflammatory *** CSS stimulates osteogenesis in vitro and in *** 4 weeks in a calvarial critical-sized bone defect model,the CSS accelerated ECM deposition(8-fold)and mineralized osteoid(69-fold)compared to the *** spatial transcriptomics,we demonstrated the comprehensive biological processes of CSS for prompt *** material-centric approach delivers impressive osteogenic properties and streamlined manufacturing advantages,potentially expediting clinical application for bone reconstruction surgeries.

关键词： Craniofacial bone regeneration composite scaffold Material-centric approach3D printing Citrate biomaterial

Comparing the regeneration potential between PLLA/Aragonite and PLLA/ Vaterite pearl composite scaffolds in rabbit radius segmental bone defects

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Bioactive Materials 2020年第4期5卷 980-989页

作者： Qianli Huang Yuansheng Liu Zhengxiao Ouyang Qingling Feng State Key Laboratory of Powder Metallurgy Central South UniversityChangsha410083PR China State Key Laboratory of New Ceramics and Fine Processing School of Materials Science and EngineeringTsinghua UniversityBeijing100084PR China Public Security College Northwest University of Political Science and LawXi'an710122PR China Department of Orthopedics The Second Xiangya HospitalCentral South UniversityChangsha410083PR China

Mussel-derived nacre and pearl,which are natural composites composed CaCO3 platelets and interplatelet organic matrix,have recently gained interest due to their osteogenic *** crystal form of CaCO3 could be either aragonite or vaterite depending on the characteristics of mineralization template within *** far,little attention has been paid on the different osteogenic capacities between aragonite and vaterite *** the current work,aragonite or vaterite pearl powders were incorporated into poly-L-lactic acid(PLLA)scaffold as bio-functional fillers for enhanced *** intro results revealed that PLLA/aragonite scaffold possessed stronger stimulatory effect on SaOS-2 cell proliferation and differentiation,evidenced by the enhanced cell viability,alkaline phosphatase activity,collagen synthesis and gene expressions of osteogenic markers including osteocalcin,osteopotin and bone *** bone regeneration potential of various scaffolds was evaluated in vivo employing a rabbit critical-sized radial bone defect *** X-ray and micro-CT results showed that significant bone regeneration and bridging were achieved in defects implanted with composite scaffolds,while less bone formation and non-bridging were found for pure PLLA *** evaluation using Masson's trichrome and hematoxylin/eosin(H&E)staining indicated a typical endochondral bone formation process conducted at defect sites treated with composite *** three-point bending test,the limbs implanted with PLLA/aragonite scaffold were found to bear significantly higher bending load compared to other two ***,it is suggested that aragonite pearl has superior osteogenic capacity over vaterite pearl and PLLA/aragonite scaffold can be employed as a potential bone graft for bone regeneration.

关键词： composite scaffold Aragonite Vaterite Segmental bone defect Bone regeneration

Synthesis and characterization of collagen/PLGA biodegradable skin scaffold fibers

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Regenerative Biomaterials 2017年第5期4卷 309-314页

作者： Alireza Sadeghi-Avalshahr Samira Nokhasteh Amir Mahdi Molavi Mohammad Khorsand-Ghayeni Meysam Mahdavi-Shahri Materials Research Department Iranian Academic Center for EducationCulture and Research(ACECR)Khorasan Razavi BranchMashhadIran

The aim of this study is to investigate the applicability of poly(lactic-co-glycolic acid)(PLGA)/collagen composite scaffold for skin tissue *** and collagen were dissolved in HFIP as a common solvent and fibrous scaffolds were prepared by electrospinning *** scaffolds were characterized by scanning electron microscopy(SEM),FTIR spectroscopy,mercury porosimetry,tensile strength,biocompatibility assays and *** and cell adhesion were tested for two cell line groups,human dermal fibroblast(HDF)and human keratinocyte(HaCat).SEM images showed appropriate cell adhesion to the scaffold for both cell *** assays indicated that the cell viability of HDF cells increased with time,but the number of HaCat cells decreased after 14 *** ultimate tensile strength was suitable for skin substitute application,but its elongation at break was rather *** successful clinical application of the PLGA/collagen scaffold,some properties especially mechanical strain needs to be improved.

关键词： tissue engineering composite scaffold biocompatibility skin cells

Biomimetic 3D printing of composite structures with decreased cracking

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Nanotechnology and Precision Engineering 2024年第3期7卷 24-34页

作者： Fan Du Kai Li Mingzhen Li Junyang Fang Long Sun Chao Wang Yexin Wang Maiqi Liu Jinbang Li Xiaoying Wang School of Mechanical Engineering and Mechanics Ningbo UniversityNingbo 315211China School of Mechanical and Electrical Engineering China University of Mining and TechnologyXuzhou 221116China Institute of Advanced Energy Storage Technology and Equipment Ningbo UniversityNingbo 315211China School of Civil and Environmental Engineering and Geography Science Ningbo UniversityNingbo 315211China

The development of tissue engineering and regeneration research has created new platforms for bone ***,the preparation of scaffolds with good fiber integrity is challenging,because scaffolds prepared by traditional printing methods are prone to fiber cracking during solvent *** skin has an excellent natural heat-management system,which helps to maintain a constant body temperature through perspiration or blood-vessel *** this work,an electrohydrodynamic-jet 3D-printing method inspired by the thermal-management system of skin was *** this system,the evaporation of solvent in the printed fibers can be adjusted using the temperature-change rate of the substrate to prepare 3D structures with good structural *** investigate the solvent evaporation and the interlayer bonding of the fibers,finite-element analysis simulations of a three-layer microscale structure were carried *** results show that the solvent-evaporation path is from bottom to top,and the strain in the printed structure becomes smaller with a smaller temperaturechange *** results verified the accuracy of these simulation results,and a variety of complex 3D structures with high aspect ratios were *** cracks were reduced to the nanoscale by adjusting the temperature-change rate from 2.5 to 0.5℃*** process parameters were selected to prepare a tissue engineering scaffold with high *** was confirmed that this printed scaffold had good biocompatibility and could be used for bone-tissue *** simple and flexible 3D-printing method can also help with the preparation of a wide range of micro-and nanostructured sensors and actuators.

关键词： 3D printing Electrohydrodynamic jet Biomimetic Structural integrity composite scaffold

Titanium alloy composited with dual-cytokine releasing polysaccharide hydrogel to enhance osseointegration via osteogenic and macrophage polarization signaling pathways

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Regenerative Biomaterials 2022年第1期9卷 372-388页

作者： Yaping Wang Zujian Feng Xiang Liu Chunfang Yang Rui Gao Wenshuai Liu Wenbin Ou-Yang Anjie Dong Chuangnian Zhang Pingsheng Huang Weiwei Wang Department of Polymer Science and Engineering Key Laboratory of Systems Bioengineering(Ministry of Education)School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300072China Key Laboratory of Innovative Cardiovascular Devices Chinese Academy of Medical SciencesBeijing 100037China Tianjin Key Laboratory of Biomaterial Research Institute of Biomedical EngineeringChinese Academy of Medical Sciences and Peking Union Medical CollegeTianjin 300192China Structural Heart Disease Center National Center for Cardiovascular DiseaseChina and Fuwai HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100037China

Titanium alloy has been widely used in orthopedic surgeries as bone defect ***,the regeneration of high-quality new bones is limited due to the pro-inflammatory microenvironment around implants,resulting in a high occurrence rate of implant loosening or failure in osteological *** this study,extracellular matrix-mimetic polysaccharide hydrogel co-delivering BMP-2 and interleukin(IL)-4 was composited with 3D printed titanium alloy to promote the osseointegration and regulate macrophage response to create a pro-healing microenvironment in bone ***,it is discovered from the bioinformatics data that IL-4 and BMP-2 could affect each other through multiple signal pathways to achieve a synergistic effect toward *** composite scaffold significantly promoted the osteoblast differentiation and proliferation of human bone marrow mesenchyme stem cells(hBMSCs).The repair of large-scale femur defect in rat indicated that the dual-cytokinedelivered composite scaffold could manipulate a lower inflammatory level in situ by polarizing macrophages to M2 phenotype,resulting in superior efficacy of mature new bone regeneration over the treatment of native titanium alloy or that with an individual ***,this work highlights the importance of M2-type macrophages-enriched immune-environment in bone *** biomimetic hydrogel–metal implant composite is a versatile and advanced scaffold for accelerating in vivo bone regeneration,holding great promise in treating orthopedic diseases.

关键词： hydrogel composite scaffold bone regeneration cytokine delivery macrophage polarization

Novel magnetic silk fibroin scaffolds with delayed degradation for potential long-distance vascular repair

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Bioactive Materials 2022年第1期7卷 126-143页

作者： Xin Liu Yuxiang Sun Bo Chen Yan Li Peng Zhu Peng Wang Sen Yan Yao Li Fang Yang Ning Gu State Key Laboratory of Bioelectronics Jiangsu Key Laboratory for Biomaterials and DevicesSchool of Biological Science&Medical EngineeringSoutheast UniversityNanjing210096PR China Materials Science and Devices Institute Suzhou University of Science and Technology1 Kerui RoadSuzhou215009PR China State Key Laboratory of Quality Research in Chinese Medicines Macao Institute for Applied Research in Medicine and HealthMacao University of Science and TechnologyMacao999078PR China College of Social Sciences University of GlasgowGlasgowG128QQUnited Kingdom

Although with the good biological properties,silk fibroin(SF)is immensely restrained in long-distance vascular defect repair due to its relatively fast degradation and inferior mechanical *** is necessary to construct a multifunctional composite scaffold based on *** this study,a novel magnetic SF scaffold(MSFCs)was prepared by an improved infiltration *** with SF scaffold(SFC),MSFCs were found to have better crystallinity,magnetocaloric properties,and mechanical strength,which was ascribed to the rational introduction of iron-based magnetic nanoparticles(MNPs).Moreover,in vivo and in vitro experiments demonstrated that the degradation of MSFCs was significantly *** mechanism of delayed degradation was correlated with the dual effect that was the newly formed hydrogen bonds between SFC and MNPs and the complexing to tyrosine(Try)to inhibit hydrolase by internal iron ***,theβ-crystallization of protein in MSFCs was increased with the rise of iron concentration,proving the beneficial effect after MNPS ***,although macrophages could phagocytose the released MNPs,it did not affect their function,and even a reasonable level might cause some cytokines to be ***,in vitro and in vivo studies demonstrated that MSFCs showed excellent biocompatibility and the growth promotion effect on CD34-labeled vascular endothelial cells(VECs).In conclusion,we confirm that the doping of MNPs can significantly reduce the degradation of SFC and thus provide an innovative perspective of multifunctional biocomposites for tissue engineering.

关键词： Silk fibroin scaffold Iron based magnetic nanoparticles composite scaffold Extended degradation Vascular tissue engineering